##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841117.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7276080
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.610593753779504	39.0	35.0	39.0	32.0	39.0
2	37.273715654583235	39.0	37.0	39.0	33.0	39.0
3	37.399909429253114	39.0	37.0	39.0	35.0	39.0
4	37.922863547404646	39.0	37.0	40.0	35.0	41.0
5	38.60253776758914	40.0	37.0	41.0	35.0	41.0
6	38.63413774999725	40.0	37.0	41.0	35.0	41.0
7	38.63990665303295	40.0	37.0	41.0	35.0	41.0
8	38.54184272300469	40.0	37.0	41.0	35.0	41.0
9	38.506959791536104	40.0	37.0	41.0	35.0	41.0
10	38.49648258402876	40.0	37.0	41.0	35.0	41.0
11	38.42916859077965	40.0	36.0	41.0	34.0	41.0
12	38.34647969785929	40.0	36.0	41.0	34.0	41.0
13	38.26070397796616	40.0	36.0	41.0	34.0	41.0
14	38.2496520104232	40.0	36.0	41.0	34.0	41.0
15	38.17573432397665	40.0	36.0	41.0	34.0	41.0
16	38.12636364635903	40.0	35.0	41.0	34.0	41.0
17	38.19031923783136	40.0	35.0	41.0	34.0	41.0
18	38.194753905949355	40.0	35.0	41.0	35.0	41.0
19	38.20555189057844	40.0	35.0	41.0	35.0	41.0
20	38.20016052599752	40.0	35.0	41.0	34.0	41.0
21	38.219064111444624	40.0	35.0	41.0	34.0	41.0
22	38.2108359171422	40.0	35.0	41.0	34.0	41.0
23	38.17732460335785	40.0	35.0	41.0	34.0	41.0
24	38.10261033413596	40.0	35.0	41.0	34.0	41.0
25	38.024833479690045	40.0	35.0	41.0	34.0	41.0
26	37.93535820531223	40.0	35.0	41.0	34.0	41.0
27	37.929359784156226	40.0	35.0	41.0	34.0	41.0
28	37.91587835605263	40.0	35.0	41.0	34.0	41.0
29	37.87556755573121	40.0	35.0	41.0	34.0	41.0
30	37.881165023637365	40.0	35.0	41.0	34.0	41.0
31	37.8450477017062	39.0	35.0	41.0	34.0	41.0
32	37.72558537957302	39.0	35.0	41.0	34.0	41.0
33	37.71637103567689	39.0	35.0	41.0	34.0	41.0
34	37.698149972272	39.0	35.0	41.0	34.0	41.0
35	37.62480829811939	39.0	35.0	41.0	34.0	41.0
36	37.62430616645544	39.0	35.0	41.0	34.0	41.0
37	37.55784268162883	39.0	35.0	41.0	34.0	41.0
38	37.49306530965094	39.0	35.0	41.0	34.0	41.0
39	37.49331249495101	39.0	35.0	41.0	34.0	41.0
40	37.40796416027703	38.0	35.0	41.0	34.0	41.0
41	37.36168485152187	38.0	35.0	41.0	34.0	41.0
42	37.25784752368458	38.0	35.0	41.0	34.0	41.0
43	37.130018188399674	37.0	35.0	40.0	34.0	41.0
44	37.00153339427453	37.0	35.0	40.0	33.0	41.0
45	36.79459382993529	37.0	35.0	40.0	33.0	41.0
46	36.55951326123623	37.0	35.0	40.0	32.0	41.0
47	36.26030580800463	36.0	35.0	40.0	32.0	41.0
48	36.32400516971979	37.0	35.0	40.0	32.0	41.0
49	36.275158406533194	37.0	34.0	40.0	31.0	41.0
50	36.316369936365334	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	48.0
22	119.0
23	310.0
24	856.0
25	1879.0
26	3973.0
27	8280.0
28	16024.0
29	30201.0
30	53403.0
31	94187.0
32	159798.0
33	310753.0
34	857672.0
35	353125.0
36	486739.0
37	801823.0
38	988659.0
39	1345245.0
40	1762971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.62767314268123	17.63538883574672	31.736938021572055
2	23.829672021198228	22.267319765588063	31.73664940462447	22.16635880858924
3	21.086821475299885	23.47772426911194	29.593723543446472	25.841730712141704
4	21.15632318501171	23.847923607217073	34.101851546437096	20.893901661334123
5	15.76215489659267	26.15680421325769	32.69679552726193	25.384245362887707
6	21.487545491528408	28.692688920407694	29.211003727281724	20.60876186078218
7	24.532839111169753	27.607571659464984	24.281962265395652	23.57762696396961
8	26.007094479444977	25.18281822080021	27.233098041802727	21.576989257952086
9	23.2271497839496	27.83617002561819	26.00315004782795	22.93353014260426
10	23.517319765588063	24.70003078580774	28.5197523941463	23.262897054457895
11	25.544510230783608	25.15886301415048	26.120603401831755	23.17602335323416
12	23.638827500522257	27.908406724500008	26.98997262262097	21.462793152356763
13	25.106692064958054	25.65571021758969	26.557500742157863	22.68009697529439
14	21.34903684401491	28.249565700212205	26.135790150740508	24.26560730503238
15	21.687941858803093	29.621224615452274	27.877249837824763	20.813583687919866
16	24.11105980143154	30.84034260206045	23.718100955459533	21.330496641048477
17	22.945390924783673	33.43900836714275	23.12524051412299	20.49036019395059
18	22.540502578311397	28.291937416850832	24.78139327769898	24.38616672713879
19	24.52779518641906	25.75737210146123	25.251811414937713	24.463021297182
20	25.156554078569783	27.180734681311915	25.380974370815057	22.281736869303252
21	23.843608096667438	27.858462248903255	24.517831029895216	23.78009862453409
22	26.8011071895856	28.755387516354958	23.077055227540104	21.366450066519334
23	27.534853932337196	28.726704489230464	22.846532748402986	20.891908830029358
24	25.162876164088356	28.77963133995228	22.365504502424383	23.69198799353498
25	24.779879460455764	27.459655364935387	26.67950042036271	21.080964754246136
26	25.54600474501958	29.398450087163702	25.11687605735674	19.93866911045998
27	26.172623378550984	28.23681356442037	25.89435331733665	19.696209739691994
28	24.45603007059617	27.395937302032202	26.100658221559858	22.047374405811766
29	25.06542204249135	27.984247203746303	25.7972971981425	21.15303355561985
30	26.589244705661464	29.48362288577961	24.085386158680635	19.841746249878298
31	26.415814795664254	27.33118233072842	26.051223216443287	20.201779657164042
32	24.081587335433802	27.53546100594479	27.675121230280546	20.707830428340866
33	24.69033281416037	25.412701117934056	29.248170835447695	20.648795232457886
34	23.57589694445731	27.60892592289122	26.429105377094892	22.386071755556586
35	21.04892236463087	25.429104535987207	28.65416600798052	24.8678070914014
36	24.4157620196931	24.70225391772735	28.097069245911328	22.784914816668216
37	21.563179965590717	28.720514859468395	26.135778172787226	23.58052700215366
38	23.60468116114763	26.130619301869114	26.939322025204167	23.32537751177909
39	22.523606563031468	28.699707937898754	25.400840332829166	23.375845166240612
40	19.66176162795904	27.902108271673164	28.763299162030805	23.672830938336997
41	21.08371938231148	29.482129543389675	26.47026972276822	22.963881351530627
42	22.54391353071442	29.81951143017781	26.318790470325904	21.317784568781864
43	21.512674988094318	26.838736656823407	28.639007920029552	23.009580435052722
44	22.997144880604736	27.339384153347527	23.864053889502472	25.799417076545268
45	21.851632361466468	25.856925652297765	29.71127240607619	22.580169580159577
46	22.166419712639826	28.945405254571842	26.6877703331096	22.20040469967873
47	24.14149411642367	26.99960772725822	26.762411416887083	22.09648673943103
48	25.050540573204465	28.34358924594909	24.484060495146863	22.121809685699585
49	20.013610815962853	30.295043178181157	25.860661589498534	23.830684416357457
50	21.626284298209384	29.855003600238312	26.35513335502005	22.16357874653226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	248530.0
1	244915.0
2	158592.0
3	75222.5
4	70810.5
5	48257.0
6	19392.0
7	10840.0
8	14556.0
9	17425.0
10	17574.0
11	15672.0
12	13439.0
13	12561.5
14	13259.0
15	13773.5
16	12719.0
17	12854.0
18	15638.0
19	18330.5
20	21278.0
21	28615.5
22	37355.5
23	50972.5
24	66662.5
25	74193.5
26	81748.0
27	95922.0
28	107516.5
29	118095.0
30	131464.5
31	148999.0
32	190111.5
33	231409.0
34	246561.5
35	255338.0
36	264703.5
37	276125.0
38	303483.5
39	338233.5
40	360816.5
41	382318.5
42	414322.5
43	439444.5
44	463467.5
45	467986.5
46	494570.5
47	570829.5
48	612878.5
49	635094.5
50	702186.0
51	736296.5
52	714116.5
53	680940.0
54	589480.0
55	491724.5
56	459068.5
57	440913.0
58	415864.5
59	381439.0
60	313673.5
61	242981.5
62	192193.5
63	141903.0
64	101618.5
65	84923.0
66	61733.0
67	38685.0
68	27104.5
69	18653.0
70	12928.5
71	8257.0
72	5269.5
73	3542.0
74	1958.0
75	1190.0
76	939.5
77	774.5
78	520.5
79	340.0
80	251.0
81	158.5
82	100.0
83	52.0
84	16.0
85	11.0
86	8.5
87	7.0
88	5.0
89	4.5
90	5.5
91	5.5
92	4.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	59693.0
25	71211.0
26	57457.0
27	66375.0
28	70705.0
29	58796.0
30	51312.0
31	40456.0
32	34828.0
33	28375.0
34	25279.0
35	25191.0
36	28896.0
37	29236.0
38	18032.0
39	21571.0
40	23222.0
41	29020.0
42	41626.0
43	74402.0
44	220851.0
45	558820.0
46	784411.0
47	909486.0
48	1250444.0
49	1328420.0
50	1367965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.357698397664715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5426088979839	6.601164582517526
2	11.809305568921987	2.210158395996685
3	4.741255142466628	1.3310170694873862
4	2.460076831962128	0.9208262811433635
5	1.4511520230239736	0.6789721480309673
6	1.0382221800041673	0.5829222018146977
7	0.7286851868397333	0.47731713437045287
8	0.5639406207793043	0.4221748994755632
9	0.47219026634627104	0.39767526889032373
>10	3.9463951367461836	7.50764225141501
>50	0.48718358112474125	3.238084105547208
>100	1.3878555276182951	35.28628166389552
>500	0.293561874279579	17.301809819155796
>1k	0.06951211047473771	12.709747036731054
>5k	0.004176693333246043	2.6263769416746046
>10k+	0.0038783580951570395	7.707830199853843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	32080	0.4408967465998175	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	30988	0.425888665325285	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	29471	0.4050395267781553	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	28435	0.39080109069718855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	25050	0.3442787874789722	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23930	0.32888588360765686	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	21182	0.29111829446625104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	18752	0.25772119053116516	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	16830	0.2313058679948544	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15423	0.2119685325065145	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	11916	0.1637695022594584	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	11131	0.15298072588536685	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	10907	0.14990214511110378	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	9419	0.1294515728249277	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8908	0.12242856043364009	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8746	0.12020208683796771	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	8042	0.11052654726171234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0775142659234093	0.0	0.0	0.0	0.0
2	0.12295081967213115	0.0	0.0	0.0	0.0
3	0.16000373827665446	0.0	0.0	0.0	0.0
4	0.21963749711383052	0.0	0.0	0.0	0.0
5	0.25868324702312234	0.0	0.0	0.0	0.0
6	0.34004573891436046	0.0	0.0	0.0	0.0
7	0.3806582665391255	0.0	0.0	0.0	0.0
8	0.4258474343327726	0.0	0.0	0.0	0.0
9	0.4783757187936361	0.0	0.0	0.0	0.0
10	0.5196341986344295	0.0	0.0	0.0	0.0
11	0.5645484931446603	0.0	0.0	0.0	0.0
12	0.6045288122175677	0.0	0.0	0.0	0.0
13	0.6455124187749447	0.0	0.0	0.0	0.0
14	0.6837472924981584	0.0	0.0	0.0	0.0
15	0.723329045310112	0.0	0.0	0.0	4.123099251245176E-5
16	0.7579081043638882	0.0	0.0	0.0	4.123099251245176E-5
17	0.8006234126067883	0.0	0.0	0.0	6.871832085408626E-5
18	0.8289078734703301	0.0	0.0	0.0	6.871832085408626E-5
19	0.8583468021242208	0.0	0.0	0.0	6.871832085408626E-5
20	0.8945063825576409	0.0	0.0	0.0	6.871832085408626E-5
21	0.9241102351815813	0.0	0.0	0.0	6.871832085408626E-5
22	0.9518174621499489	0.0	0.0	0.0	6.871832085408626E-5
23	0.979634638431683	0.0	0.0	0.0	6.871832085408626E-5
24	1.0074380710492463	0.0	0.0	0.0	6.871832085408626E-5
25	1.0357775065694714	0.0	0.0	0.0	6.871832085408626E-5
26	1.0681988103484295	0.0	0.0	0.0	6.871832085408626E-5
27	1.095383778078306	0.0	0.0	0.0	6.871832085408626E-5
28	1.122609976800695	0.0	0.0	0.0	6.871832085408626E-5
29	1.1507982320150412	0.0	0.0	0.0	6.871832085408626E-5
30	1.1820925553319919	0.0	0.0	0.0	6.871832085408626E-5
31	1.209772294972018	0.0	0.0	0.0	6.871832085408626E-5
32	1.2372596233136524	0.0	0.0	0.0	6.871832085408626E-5
33	1.2654203911996569	0.0	0.0	0.0	6.871832085408626E-5
34	1.294749370540181	0.0	0.0	0.0	6.871832085408626E-5
35	1.3206561775021715	0.0	0.0	0.0	6.871832085408626E-5
36	1.3429758881155787	0.0	0.0	0.0	6.871832085408626E-5
37	1.3599768006948796	0.0	0.0	0.0	6.871832085408626E-5
38	1.3733631295972557	0.0	0.0	0.0	6.871832085408626E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	90	0.0	202.96558	44
CCCGTTG	160	0.0	167.8943	44
ATCTCGT	70	0.0	153.50337	44
TCGCACA	65	0.0	148.78018	44
ATGACGT	130	0.0	148.78018	44
ATCCGTT	45	7.075869E-10	143.2698	44
ACCGACC	100	0.0	118.19759	44
GAAACGA	200	0.0	118.19759	44
GACGTTC	235	0.0	109.73857	44
AGTCCCG	65	0.0	108.27212	43
CTATCAT	3410	0.0	107.452354	44
CGTTATT	145	0.0	97.07155	43
CACCCGC	45	1.697911E-5	95.51321	44
CGGAGTT	185	0.0	92.93177	44
CGTCGGA	210	0.0	92.10202	44
GCACCTC	275	0.0	89.86924	44
ATCGCAC	55	0.0	88.58628	43
AACCGCT	185	0.0	87.123535	44
CGTTTAA	275	0.0	85.96188	44
AACGACA	3095	0.0	85.70814	43
>>END_MODULE