FastQCFastQC Report
Tue 24 May 2016
ERR841116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862421
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA233850.6054492765030016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA222930.5771768535848371No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA219310.5678044936064712No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG207530.5373054879310153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA193190.5001785149780409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA166990.4323454123721883No Hit
AAAAAAAAAAAAAAAAAAAAAAAA151200.39146431732843207No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC109930.28461423547562525No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA108310.28041997493282067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107320.2778568157122178No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA101840.26366882325877994No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA98910.25608290758568264No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG89710.23226365018210082No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA85550.22149320335613337No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT63800.16518137199440452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60460.15653394593701722No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.1466955570094508No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA55980.14493500320135996No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC53600.13877306487304208No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA53270.13791867846617445No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.13778922598028542No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA53080.13742675901979612No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA51870.13429400886128157No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT51430.13315482698545808No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA51080.13224865958423487No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49980.12940070489467617No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT49960.12934892390032054No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC46690.12088273132317787No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA44380.11490202647510461No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC41540.1075491252766076No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT39920.10335486473380298No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38660.10009266208939936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTAA1050.0210.4795244
TACTATG100.0023256473210.479544
ACCGTTT1700.0204.2889344
TCGACGC1150.0201.3282244
TTACGTT4500.0201.1248644
CATTCGA850.0198.0983644
CTATACG800.0197.3245444
CATACGT800.0197.3245444
TGCACCG700.0195.4452544
CTACCGC600.0192.9395644
CACGGGT1150.0192.1769444
TCGTCAC1050.0190.4338544
CAAGTAT900.0187.092944
AATCGCA351.4188117E-10180.4110144
CCTCGGG700.0165.3767544
AGCATGC1600.0164.4371244
TGACTCG208.7665176E-5157.8596344
TTGGGCC2550.0156.8278544
ACATTGG3550.0145.260544
GGCTCCG2250.0144.99744
Produced by FastQC (version 0.11.5)