##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841116.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3862421
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.36675546244182	39.0	35.0	39.0	31.0	39.0
2	37.23118453425973	39.0	37.0	39.0	33.0	39.0
3	37.14769570691543	39.0	35.0	39.0	33.0	39.0
4	37.76241352250312	39.0	37.0	39.0	34.0	41.0
5	38.498278929200104	40.0	37.0	41.0	34.0	41.0
6	38.45187331986855	40.0	37.0	41.0	34.0	41.0
7	38.5092738466366	40.0	37.0	41.0	34.0	41.0
8	38.36297104846934	40.0	37.0	41.0	34.0	41.0
9	38.426914880589145	40.0	37.0	41.0	34.0	41.0
10	38.420029302864705	40.0	37.0	41.0	34.0	41.0
11	38.39839339108813	40.0	37.0	41.0	34.0	41.0
12	38.22358411990821	40.0	36.0	41.0	34.0	41.0
13	38.0996147235115	40.0	36.0	41.0	34.0	41.0
14	38.060163042816924	39.0	36.0	41.0	34.0	41.0
15	37.93354168279429	39.0	36.0	41.0	34.0	41.0
16	37.82265268338175	39.0	35.0	41.0	34.0	41.0
17	37.91722497366289	39.0	35.0	41.0	34.0	41.0
18	38.00535933291581	40.0	35.0	41.0	34.0	41.0
19	38.019993159730646	40.0	35.0	41.0	34.0	41.0
20	38.01547164330351	40.0	35.0	41.0	34.0	41.0
21	37.89779855691547	40.0	35.0	41.0	34.0	41.0
22	37.94594090079771	40.0	35.0	41.0	34.0	41.0
23	37.91996134030961	40.0	35.0	41.0	34.0	41.0
24	37.81825414681621	40.0	35.0	41.0	34.0	41.0
25	37.79993977370625	40.0	35.0	41.0	33.0	41.0
26	37.74629401012254	40.0	35.0	41.0	33.0	41.0
27	37.77941841254015	40.0	35.0	41.0	33.0	41.0
28	37.70456295836025	39.0	35.0	41.0	33.0	41.0
29	37.62785772960221	39.0	35.0	41.0	33.0	41.0
30	37.579526574805364	39.0	35.0	41.0	33.0	41.0
31	37.51583183506892	39.0	35.0	41.0	33.0	41.0
32	37.28605769371109	39.0	35.0	41.0	33.0	41.0
33	37.26852884173152	39.0	35.0	41.0	33.0	41.0
34	37.24947262350687	38.0	35.0	41.0	33.0	41.0
35	37.23262314313357	38.0	35.0	41.0	33.0	41.0
36	37.23859889926678	38.0	35.0	41.0	33.0	41.0
37	37.22588649941538	38.0	35.0	41.0	33.0	41.0
38	37.11621416024505	38.0	35.0	41.0	33.0	41.0
39	37.08723123413693	38.0	35.0	41.0	33.0	41.0
40	37.008708936320126	37.0	35.0	40.0	33.0	41.0
41	36.90577139296131	37.0	35.0	40.0	33.0	41.0
42	36.85406104726691	37.0	35.0	40.0	33.0	41.0
43	36.74493224763819	37.0	35.0	40.0	33.0	41.0
44	36.588430797228575	37.0	35.0	40.0	33.0	41.0
45	36.39555652101663	36.0	35.0	40.0	32.0	41.0
46	36.120311092263854	36.0	35.0	40.0	31.0	41.0
47	35.90157214869746	36.0	34.0	39.0	31.0	41.0
48	35.93397243231737	36.0	34.0	39.0	31.0	41.0
49	35.83640900676435	36.0	34.0	39.0	31.0	41.0
50	36.027077277717716	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	7.0
21	32.0
22	81.0
23	216.0
24	560.0
25	1218.0
26	2435.0
27	5105.0
28	9913.0
29	18880.0
30	34125.0
31	60470.0
32	103029.0
33	186349.0
34	400157.0
35	213846.0
36	354514.0
37	535346.0
38	519662.0
39	687875.0
40	728598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.57401070468496	20.391277905748755	30.034711389566286
2	25.134235755242628	24.215848039351485	30.88068338485111	19.769232820554777
3	21.365174847589117	24.58942202313005	32.06217033306312	21.98323279621771
4	22.653304753676515	25.386693993223417	31.42570424094111	20.53429701215895
5	17.25428170569702	26.211306328336555	33.221676249171175	23.312735716795245
6	18.02874414777674	30.813212749205743	29.915252635587887	21.242790467429625
7	25.74165270953115	26.653749034608087	24.629008593314918	22.975589662545847
8	24.610988807279167	25.923973590657255	30.335895543235708	19.12914205882787
9	26.26795473616159	26.27093214333704	26.254569349120665	21.206543771380694
10	21.327555955189766	25.723089223054657	26.71215799624122	26.23719682551436
11	25.863286265272478	25.07574912211797	29.692853264830532	19.36811134777902
12	26.767123521749703	27.702365951303598	26.511739657587817	19.01877086935888
13	21.9464941807224	27.19768248981662	24.973999468209186	25.88182386125179
14	19.384137565532082	26.674461432350334	30.951027865683205	22.990373136434375
15	24.725864943257093	31.564399634322616	24.598613149628175	19.111122272792116
16	21.408411977876053	30.54081882839804	28.26654577530518	19.784223418420726
17	25.696137215492566	31.659857897417186	23.517710782951937	19.12629410413831
18	23.979933829067313	29.88288951411563	23.03871069466534	23.098465962151717
19	25.70328299271364	28.565166769753997	22.917569058370386	22.81398117916198
20	25.04926314350507	27.677847650476217	25.584212596192906	21.688676609825805
21	21.38166709429138	34.57668130946885	24.620930758195442	19.420720838044325
22	21.35859866130595	32.815972158394956	23.49914729647545	22.326281883823643
23	24.599053288080196	32.298421119810605	25.05115314979905	18.051372442310146
24	23.46007853623414	34.30304464479662	23.58520730909448	18.651669509874765
25	20.772572422118238	32.43479194434043	26.784622917937746	20.008012715603584
26	20.994638322987168	35.437357227171205	24.697735591090126	18.870268858751498
27	24.59464998413921	31.745275404329337	23.78506463978005	19.875009971751407
28	22.362365881509703	30.54026211869742	25.598706030801267	21.498665968991617
29	22.76935518379374	31.82352054328318	24.64686815117657	20.760256121746515
30	26.5392838595237	31.15044705385105	23.478842223825936	18.83142686279932
31	22.607653795010023	33.74746141957246	24.14342001666028	19.501464768757234
32	20.579429998604684	32.46253516752264	27.341519190272717	19.616515643599964
33	20.399576943794727	31.01043144932542	29.143543829446074	19.446447777433782
34	20.62169546219294	31.912110915508794	27.322181002450197	20.14401261984807
35	19.49604605146329	33.34115208855735	26.350551780007965	20.812250079971395
36	19.924568612734213	31.029812433992987	26.551824066989766	22.49379488628303
37	21.90524391786932	34.71550156331224	23.618682915154295	19.760571603664143
38	22.2949231401148	32.20661577670633	25.6696728704283	19.82878821275057
39	20.788314042069498	31.190385243155085	25.402790111008333	22.61851060376709
40	20.49617553583435	31.334338552533968	26.756022011447346	21.413463900184336
41	19.206583645051857	32.3153007273727	27.474772565357913	21.00334306221753
42	19.686466485014652	32.26475325705959	25.05522290386235	22.993557354063405
43	21.017009992018686	30.632698897805284	24.74434910770712	23.60594200246891
44	20.733070893465094	31.66519510129382	25.059601610625087	22.542132394615997
45	21.03721885812427	31.704017663933453	25.978288471093396	21.280475006848878
46	19.659773558991755	30.676322299861187	26.466319981452735	23.197584159694323
47	20.228724461472034	30.89283756091209	26.14152336762458	22.736914609991295
48	23.745778170949052	30.027828722577212	23.674406535528625	22.551986570945115
49	21.38133026678653	32.0429042753686	25.00034224852719	21.57542320931768
50	23.705202466134125	27.20501527662683	27.45516215378705	21.634620103451997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185430.0
1	186861.5
2	135201.0
3	81273.5
4	76373.0
5	52524.5
6	21556.0
7	10962.0
8	11381.5
9	11402.0
10	11639.5
11	10955.5
12	9875.0
13	9519.5
14	9667.5
15	9981.5
16	9737.5
17	10789.5
18	15549.0
19	19712.0
20	22029.0
21	28601.0
22	35804.5
23	41138.5
24	49600.0
25	58963.5
26	73534.0
27	98469.0
28	118416.5
29	123523.0
30	128367.0
31	138071.0
32	151703.0
33	157566.0
34	147887.0
35	147840.5
36	158452.0
37	162783.0
38	164110.5
39	160537.5
40	164978.0
41	189145.5
42	207078.0
43	220721.5
44	239896.0
45	252762.5
46	257160.0
47	258119.0
48	270654.5
49	287540.0
50	317712.0
51	336986.0
52	324201.5
53	317605.5
54	307160.5
55	276398.0
56	249115.5
57	237665.5
58	223096.0
59	183691.0
60	136934.5
61	105214.5
62	82785.0
63	61961.0
64	42887.5
65	31527.0
66	20595.0
67	11865.0
68	8786.0
69	6995.5
70	4924.0
71	3289.0
72	2482.0
73	1501.5
74	616.5
75	326.0
76	217.5
77	130.5
78	67.5
79	43.5
80	31.0
81	24.5
82	15.5
83	8.0
84	5.5
85	18.5
86	29.5
87	26.5
88	24.5
89	24.0
90	12.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	43491.0
25	50706.0
26	45207.0
27	53687.0
28	61046.0
29	49876.0
30	55466.0
31	77196.0
32	73000.0
33	18316.0
34	17619.0
35	17919.0
36	18997.0
37	22409.0
38	16468.0
39	16514.0
40	19957.0
41	21538.0
42	29392.0
43	41285.0
44	115491.0
45	270833.0
46	391496.0
47	403773.0
48	615905.0
49	601653.0
50	713181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.411426968231305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47340061610402	9.243211728226072
2	10.446121558934383	2.593025496599613
3	4.034980855532208	1.502396106199484
4	2.093018746860101	1.0390939727917259
5	1.3483713790202678	0.8367606448381693
6	0.868870810171077	0.6470355963159772
7	0.6805115520179736	0.5912283600236172
8	0.5131303306783277	0.5094943699518755
9	0.3903694395945437	0.4360537611141354
>10	3.3796605209880646	8.617445691250449
>50	0.517026172093219	4.536669554828183
>100	1.0161355733134667	32.26846057447008
>500	0.18355525649635113	14.438459124111864
>1k	0.045705990163434046	10.482862369796052
>5k	0.005119070898304614	3.9873642416155644
>10k+	0.004022127134382197	8.270438407867115
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	23385	0.6054492765030016	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	22293	0.5771768535848371	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	21931	0.5678044936064712	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	20753	0.5373054879310153	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	19319	0.5001785149780409	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16699	0.4323454123721883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	15120	0.39146431732843207	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	10993	0.28461423547562525	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	10831	0.28041997493282067	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10732	0.2778568157122178	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	10184	0.26366882325877994	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	9891	0.25608290758568264	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	8971	0.23226365018210082	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	8555	0.22149320335613337	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	6380	0.16518137199440452	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6046	0.15653394593701722	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5666	0.1466955570094508	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	5598	0.14493500320135996	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5360	0.13877306487304208	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5327	0.13791867846617445	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5322	0.13778922598028542	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	5308	0.13742675901979612	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5187	0.13429400886128157	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5143	0.13315482698545808	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5108	0.13224865958423487	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4998	0.12940070489467617	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	4996	0.12934892390032054	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC	4669	0.12088273132317787	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	4438	0.11490202647510461	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4154	0.1075491252766076	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	3992	0.10335486473380298	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3866	0.10009266208939936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.14019704221782142	0.0	0.0	0.0	0.0
2	0.20259314041633472	0.0	0.0	0.0	0.0
3	0.26071730658051	0.0	0.0	0.0	0.0
4	0.34763170560640594	0.0	0.0	0.0	0.0
5	0.3951407679276806	0.0	0.0	0.0	0.0
6	0.5490856641469172	0.0	0.0	0.0	0.0
7	0.6077535307518264	0.0	0.0	0.0	0.0
8	0.6908620266925848	0.0	0.0	0.0	0.0
9	0.7724429832998526	0.0	0.0	0.0	0.0
10	0.8247417875990214	0.0	0.0	0.0	0.0
11	0.8940506485440091	0.0	0.0	0.0	0.0
12	0.9658967782124217	0.0	0.0	0.0	0.0
13	1.0229853244894846	0.0	0.0	0.0	0.0
14	1.0741449469128301	0.0	0.0	0.0	0.0
15	1.1296023918676912	0.0	0.0	0.0	0.0
16	1.1948723352529411	0.0	0.0	0.0	0.0
17	1.2527117059481605	0.0	0.0	0.0	0.0
18	1.3004796732412132	0.0	0.0	0.0	0.0
19	1.3474968161161096	0.0	0.0	0.0	0.0
20	1.4135952554110491	0.0	0.0	0.0	0.0
21	1.4890919451815325	0.0	0.0	0.0	0.0
22	1.540096224621811	0.0	0.0	0.0	0.0
23	1.591825437983068	0.0	0.0	0.0	0.0
24	1.6395416242817653	0.0	0.0	0.0	0.0
25	1.6865846576538395	0.0	0.0	0.0	0.0
26	1.7349739968791595	0.0	0.0	0.0	0.0
27	1.7745605670640254	0.0	0.0	0.0	0.0
28	1.8155711145936706	0.0	0.0	0.0	0.0
29	1.8568923480894497	0.0	0.0	0.0	0.0
30	1.8963494657884266	0.0	0.0	0.0	0.0
31	1.9344343871369796	0.0	0.0	0.0	0.0
32	1.9677554570048164	0.0	0.0	0.0	0.0
33	2.0007658409065194	0.0	0.0	0.0	0.0
34	2.032404028457799	0.0	0.0	0.0	0.0
35	2.061919195240498	0.0	0.0	0.0	0.0
36	2.085039409220279	0.0	0.0	0.0	0.0
37	2.102463713820943	0.0	0.0	0.0	0.0
38	2.11678115876027	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCTAA	105	0.0	210.47952	44
TACTATG	10	0.0023256473	210.4795	44
ACCGTTT	170	0.0	204.28893	44
TCGACGC	115	0.0	201.32822	44
TTACGTT	450	0.0	201.12486	44
CATTCGA	85	0.0	198.09836	44
CTATACG	80	0.0	197.32454	44
CATACGT	80	0.0	197.32454	44
TGCACCG	70	0.0	195.44525	44
CTACCGC	60	0.0	192.93956	44
CACGGGT	115	0.0	192.17694	44
TCGTCAC	105	0.0	190.43385	44
CAAGTAT	90	0.0	187.0929	44
AATCGCA	35	1.4188117E-10	180.41101	44
CCTCGGG	70	0.0	165.37675	44
AGCATGC	160	0.0	164.43712	44
TGACTCG	20	8.7665176E-5	157.85963	44
TTGGGCC	255	0.0	156.82785	44
ACATTGG	355	0.0	145.2605	44
GGCTCCG	225	0.0	144.997	44
>>END_MODULE