FastQCFastQC Report
Tue 24 May 2016
ERR841114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9972938
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1185771.1889876383468942No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT635730.63745508094004No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC577120.5786860401618861No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC498110.49946164309855334No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT311610.31245556725610846No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA274650.2753952746923725No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG260070.2607757112297299No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT250770.2514504752761924No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG224130.22473818648025287No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA190570.1910871199640467No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174770.1752442459784669No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT168710.1691678019055167No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT167280.1677339215384674No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC157520.15794743735497002No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT137870.13824411622733443No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA135900.1362687705468539No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129120.12947037272266207No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT129030.12938012850375685No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC111960.11226380831807037No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT103230.10351011908426584No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA102100.10237705278023387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGC1050.0191.5927744
TAGTCGT1100.0154.0075844
GCCCGCT1700.0137.0214544
CTATCAT161800.0128.1294744
TCCCGCG2150.0118.1918744
ACTTCTA198050.0117.1338544
GGCTAAC2350.0112.6385344
GTAGCAC1550.0109.2957144
TTGCCCG1100.0105.8802244
GCCCGTA1800.094.11574644
TACCACG306.3775224E-790.00149543
CGCTTAT1550.088.80276544
GATCGGG3700.088.7104644
GCACTAG2000.086.40143643
CACGGTG1950.081.4463244
TCGTTAT403.7269274E-881.0013443
CCTTAGC307700.080.8170743
CGATAGT1053.8198777E-1180.6706444
CGCGATT700.077.14413543
AATCGCA7700.077.003844
Produced by FastQC (version 0.11.5)