##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841114.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9972938
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.12556550537063	39.0	37.0	39.0	32.0	39.0
2	37.75993523673766	39.0	38.0	39.0	35.0	39.0
3	37.84665210993992	39.0	37.0	39.0	35.0	39.0
4	38.372612062764254	39.0	38.0	40.0	35.0	41.0
5	38.968624491599165	40.0	38.0	41.0	35.0	41.0
6	38.9268193585481	40.0	38.0	41.0	35.0	41.0
7	38.84740444591153	40.0	38.0	41.0	35.0	41.0
8	38.78530398965681	40.0	38.0	41.0	35.0	41.0
9	38.6936811399008	40.0	38.0	41.0	35.0	41.0
10	38.64091875433298	40.0	38.0	41.0	35.0	41.0
11	38.51357212889522	40.0	38.0	41.0	34.0	41.0
12	38.42235447568209	40.0	38.0	41.0	34.0	41.0
13	38.21417349631573	40.0	37.0	41.0	34.0	41.0
14	38.12315588445451	39.0	37.0	41.0	34.0	41.0
15	38.010956951702696	39.0	37.0	41.0	34.0	41.0
16	38.114793153231275	39.0	37.0	41.0	34.0	41.0
17	38.25633078236323	40.0	37.0	41.0	34.0	41.0
18	38.262384464838746	40.0	37.0	41.0	34.0	41.0
19	38.233509122387005	40.0	37.0	41.0	34.0	41.0
20	38.15800148361496	40.0	37.0	41.0	34.0	41.0
21	38.12836267507128	40.0	36.0	41.0	34.0	41.0
22	38.06917550274553	40.0	36.0	41.0	34.0	41.0
23	38.00088258846089	40.0	36.0	41.0	34.0	41.0
24	37.84819739178164	39.0	35.0	41.0	34.0	41.0
25	37.797386921235514	39.0	35.0	41.0	34.0	41.0
26	37.70922370021968	39.0	35.0	41.0	33.0	41.0
27	37.655152409601364	39.0	35.0	41.0	33.0	41.0
28	37.44863664432173	39.0	35.0	41.0	33.0	41.0
29	37.34577714006718	39.0	35.0	41.0	33.0	41.0
30	37.318026408712896	39.0	35.0	41.0	33.0	41.0
31	37.2353320820221	38.0	35.0	40.0	33.0	41.0
32	36.99411153992371	38.0	35.0	40.0	33.0	41.0
33	36.793013419030785	37.0	35.0	40.0	33.0	41.0
34	36.7699261651379	37.0	35.0	40.0	33.0	41.0
35	36.76899619857023	37.0	35.0	40.0	33.0	41.0
36	36.72891641399099	37.0	35.0	40.0	33.0	41.0
37	36.633122641454854	37.0	35.0	40.0	33.0	41.0
38	36.506577887192094	37.0	35.0	39.0	33.0	41.0
39	36.401252970946715	36.0	35.0	39.0	33.0	41.0
40	36.29679740889704	36.0	35.0	39.0	33.0	41.0
41	36.244729249268744	36.0	35.0	39.0	33.0	41.0
42	36.171621478987134	36.0	35.0	39.0	33.0	41.0
43	35.97583892372225	35.0	35.0	39.0	33.0	41.0
44	35.81470067679575	35.0	35.0	38.0	33.0	41.0
45	35.65453928757086	35.0	35.0	38.0	32.0	41.0
46	35.43742576414102	35.0	34.0	37.0	32.0	40.0
47	35.186893793500296	35.0	34.0	37.0	31.0	40.0
48	35.18890024120359	35.0	34.0	37.0	31.0	40.0
49	35.18840155760281	35.0	34.0	37.0	31.0	40.0
50	35.48858442614727	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	19.0
21	38.0
22	119.0
23	280.0
24	730.0
25	1819.0
26	4051.0
27	9290.0
28	20583.0
29	41640.0
30	77499.0
31	136565.0
32	228567.0
33	386101.0
34	674217.0
35	859318.0
36	1312051.0
37	1452223.0
38	1713313.0
39	1852623.0
40	1201890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.89118833386912	19.198254315829498	36.91055735030139
2	22.59673127417417	19.271973815539614	34.332239907638055	23.799055002648167
3	21.144421032197332	22.624566602138707	26.697167875705237	29.533844489958728
4	18.43982184587932	25.848922353673508	30.59967885090632	25.111576949540847
5	14.76937889316067	27.086732114448118	32.0584766495089	26.085412342882307
6	19.285229688583243	24.37540472025395	28.490611292279166	27.848754298883637
7	20.105740154004767	26.192752827702325	25.0263162169463	28.675190801346606
8	22.30380856674332	22.742044520882413	30.08941798294545	24.86472892942882
9	19.183584616689686	29.227385149692097	28.08979660757943	23.499233626038787
10	25.346472624215654	20.27715403424748	30.437550098075413	23.938823243461453
11	22.58921092259874	23.398089910916923	26.15151121966265	27.861187946821687
12	21.275255095339006	24.81316939902765	27.08432560194398	26.827249903689363
13	22.97966757639524	24.21917192305818	25.343294022283104	27.457866478263476
14	18.510673584855336	24.46323239951958	25.753313617311168	31.272780398313916
15	20.789590790597515	32.16095397364347	26.45861229659705	20.59084293916196
16	20.18632824148711	32.38691547064666	24.492551743528338	22.934204544337888
17	19.40688892280289	36.468621383187184	22.079441384274123	22.045048309735808
18	18.85432357044634	28.229133681569063	23.368259183001037	29.548283564983556
19	22.36605702351704	24.94405359784649	23.80239403874766	28.887495339888808
20	22.228083639946423	22.028423319186384	28.807348446365555	26.93614459450164
21	19.824378733729215	32.03211531045314	24.771245945778468	23.372260010039167
22	23.573183749863883	24.773572241199133	24.703362238890886	26.949881770046098
23	25.398543538523953	22.580998698678364	30.16248571885236	21.857972043945324
24	20.283420993893674	28.203123292253494	25.64058856076314	25.87286715308969
25	20.82083189692668	25.220011856395846	26.59062571484724	27.36853053183023
26	22.21125304325639	33.47407151617146	24.890713685828786	19.423961754743356
27	23.731927177430883	26.650884002495552	22.674385779923835	26.942803040149734
28	21.9176545207795	24.367238425969212	25.6611129794052	28.053994073846088
29	21.90233299993673	29.156913974401487	26.717114457534596	22.22363856812719
30	24.739143001099542	28.6693784192029	26.287581719695286	20.303896860002276
31	24.014382414698908	28.046118137093863	23.89664282581733	24.042856622389905
32	19.540596121217472	29.818561455367888	25.44731390804418	25.19352851537046
33	19.53897513082133	23.00175886872364	33.943442744885274	23.51582325556975
34	20.40555270867577	29.493767384633507	25.85616933258063	24.24451057411009
35	18.41358298133377	24.28746774908026	27.22451494671875	30.074434322867223
36	20.044589956493336	27.155290150400273	26.165119745787624	26.635000147318767
37	19.22107203623865	33.372265044508325	22.254552195196634	25.152110724056392
38	24.36752116966857	25.11239737794807	24.078105539860235	26.44197591252312
39	18.699049756901385	31.92995368177562	26.39051970916323	22.98047685215977
40	17.68087958876311	25.821328891056766	29.410672603204446	27.087118916975673
41	17.966012919549094	30.435395873925025	24.545810689226695	27.05278051729919
42	17.56927396683475	32.54821513489342	25.300469979105735	24.58204091916609
43	18.84486305374081	24.767740806916006	24.649321845632187	31.738074293710994
44	20.147830085073796	27.649813940025954	23.0566761623959	29.145679812504355
45	18.813443634803946	24.975410684343295	31.642599785482822	24.568545895369933
46	18.05601556759669	27.92024632033482	29.209289942805338	24.81444816926315
47	18.88010914220361	29.818529989416298	28.91436742941767	22.386993438962417
48	23.138752011581346	26.97062365165406	22.38159316497983	27.509031171784766
49	17.637952322157847	28.095355684300504	25.245430986527005	29.021261007014637
50	16.882934597674403	33.91716957810942	26.268361515586875	22.9315343086293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83968.0
1	86145.5
2	123063.5
3	155953.0
4	144050.0
5	89295.5
6	30782.0
7	17987.0
8	19598.5
9	18965.0
10	16413.5
11	13484.5
12	10806.0
13	8706.5
14	7813.5
15	8000.0
16	8664.0
17	10571.0
18	12771.0
19	15258.5
20	18099.0
21	21061.5
22	24809.5
23	32698.5
24	41931.0
25	53683.5
26	70469.5
27	91951.5
28	108700.5
29	115959.0
30	141969.5
31	246159.5
32	415505.0
33	454682.0
34	397301.0
35	420777.0
36	428406.0
37	395350.0
38	391116.0
39	408437.0
40	450989.0
41	516556.5
42	577521.0
43	607274.5
44	622500.0
45	664120.5
46	749699.0
47	807708.0
48	898598.0
49	1038346.0
50	1218317.0
51	1332147.0
52	1156305.5
53	902024.0
54	763931.5
55	675255.0
56	627287.5
57	607171.5
58	566022.0
59	487677.5
60	374187.5
61	271278.5
62	211298.0
63	165409.5
64	130907.5
65	110843.0
66	75678.0
67	44646.5
68	32784.0
69	25545.5
70	19197.0
71	13798.0
72	9197.5
73	5544.5
74	3162.0
75	2373.0
76	1877.5
77	1185.5
78	508.0
79	383.5
80	300.5
81	195.5
82	160.0
83	85.0
84	23.5
85	13.0
86	4.0
87	1.5
88	0.5
89	0.0
90	1.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	40746.0
25	39828.0
26	44428.0
27	61825.0
28	81953.0
29	68323.0
30	111426.0
31	196843.0
32	185326.0
33	42609.0
34	35936.0
35	35653.0
36	40510.0
37	45375.0
38	26228.0
39	28594.0
40	32382.0
41	43923.0
42	53142.0
43	92940.0
44	306643.0
45	755847.0
46	1061267.0
47	1169795.0
48	1686246.0
49	1811394.0
50	1873756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.725123827020164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.47411565829495	6.06170261891344
2	12.201508999926828	2.1291935380172506
3	5.027014324226288	1.3158396737723537
4	2.6105443727695197	0.9110929163337897
5	1.5884314733733615	0.6929630647959331
6	1.0817287327232885	0.5662930284153774
7	0.7687544989269969	0.4695234737001822
8	0.5829130347059133	0.4068790726554557
9	0.4823135313771183	0.37874207562414586
>10	3.833528858390165	6.760721383408333
>50	0.5660031940533028	3.51283854572177
>100	1.4342572590500091	33.52780493711432
>500	0.26277901119312835	14.777270377734872
>1k	0.07414122101520407	12.225816317294253
>5k	0.007038723514101652	4.132018396206849
>10k+	0.0049271064598711565	12.131300580291665
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	118577	1.1889876383468942	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	63573	0.63745508094004	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	57712	0.5786860401618861	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	49811	0.49946164309855334	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	31161	0.31245556725610846	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	27465	0.2753952746923725	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	26007	0.2607757112297299	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	25077	0.2514504752761924	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	22413	0.22473818648025287	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	19057	0.1910871199640467	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17477	0.1752442459784669	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16871	0.1691678019055167	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16728	0.1677339215384674	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	15752	0.15794743735497002	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	13787	0.13824411622733443	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	13590	0.1362687705468539	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12912	0.12947037272266207	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	12903	0.12938012850375685	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	11196	0.11226380831807037	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	10323	0.10351011908426584	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	10210	0.10237705278023387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.030673007292334516	0.0	0.0	0.0	0.0
2	0.0489324209174869	0.0	0.0	0.0	0.0
3	0.06996935105783271	0.0	0.0	0.0	0.0
4	0.09678191120811139	0.0	0.0	0.0	0.0
5	0.11877141921467876	0.0	0.0	0.0	0.0
6	0.16705207632896144	0.0	0.0	0.0	0.0
7	0.1966220987235657	0.0	0.0	0.0	0.0
8	0.23471518623699456	0.0	0.0	0.0	0.0
9	0.26811557436735295	0.0	0.0	0.0	0.0
10	0.29613139076970096	0.0	0.0	0.0	0.0
11	0.3270149679061476	0.0	0.0	0.0	0.0
12	0.35639447472750757	0.0	0.0	0.0	0.0
13	0.38531273331890764	0.0	0.0	0.0	0.0
14	0.4155044381104144	0.0	0.0	0.0	0.0
15	0.44948640009593965	0.0	0.0	0.0	0.0
16	0.48183393900573734	0.0	0.0	0.0	0.0
17	0.519606158185281	0.0	0.0	0.0	0.0
18	0.549206261986187	0.0	0.0	0.0	0.0
19	0.5802903818313119	0.0	0.0	0.0	0.0
20	0.6135102815238599	0.0	0.0	0.0	0.0
21	0.6456873591312811	0.0	0.0	0.0	0.0
22	0.672018616780732	0.0	0.0	0.0	0.0
23	0.7001948673500227	0.0	0.0	0.0	0.0
24	0.7309380645903946	0.0	0.0	0.0	0.0
25	0.75856282271082	0.0	0.0	0.0	0.0
26	0.7916623967781611	0.0	0.0	0.0	0.0
27	0.8172616735409365	0.0	0.0	0.0	0.0
28	0.8522563762052867	0.0	0.0	0.0	0.0
29	0.884092531207955	0.0	0.0	0.0	0.0
30	0.9155175736578328	0.0	0.0	0.0	0.0
31	0.9420794554222637	0.0	0.0	0.0	0.0
32	0.9710979853680028	0.0	0.0	0.0	0.0
33	0.9989533676034084	0.0	0.0	0.0	0.0
34	1.0304586271367575	0.0	0.0	0.0	0.0
35	1.0562183380664756	0.0	0.0	0.0	0.0
36	1.0804438972748052	0.0	0.0	0.0	0.0
37	1.0971892134494368	0.0	0.0	0.0	0.0
38	1.1115480713907977	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGC	105	0.0	191.59277	44
TAGTCGT	110	0.0	154.00758	44
GCCCGCT	170	0.0	137.02145	44
CTATCAT	16180	0.0	128.12947	44
TCCCGCG	215	0.0	118.19187	44
ACTTCTA	19805	0.0	117.13385	44
GGCTAAC	235	0.0	112.63853	44
GTAGCAC	155	0.0	109.29571	44
TTGCCCG	110	0.0	105.88022	44
GCCCGTA	180	0.0	94.115746	44
TACCACG	30	6.3775224E-7	90.001495	43
CGCTTAT	155	0.0	88.802765	44
GATCGGG	370	0.0	88.71046	44
GCACTAG	200	0.0	86.401436	43
CACGGTG	195	0.0	81.44632	44
TCGTTAT	40	3.7269274E-8	81.00134	43
CCTTAGC	30770	0.0	80.81707	43
CGATAGT	105	3.8198777E-11	80.67064	44
CGCGATT	70	0.0	77.144135	43
AATCGCA	770	0.0	77.0038	44
>>END_MODULE