##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841113.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19392512
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.64283183117278	39.0	38.0	39.0	35.0	39.0
2	38.074757566219375	39.0	38.0	39.0	35.0	39.0
3	38.15804729165566	39.0	38.0	39.0	35.0	39.0
4	38.60877353073186	39.0	38.0	40.0	35.0	41.0
5	39.335265642738804	40.0	39.0	41.0	36.0	41.0
6	39.31380653522349	40.0	39.0	41.0	36.0	41.0
7	39.33205455796546	40.0	39.0	41.0	36.0	41.0
8	39.25536191494944	40.0	39.0	41.0	36.0	41.0
9	39.20488629838156	40.0	39.0	41.0	36.0	41.0
10	39.21357093913362	40.0	39.0	41.0	36.0	41.0
11	39.11012647562111	40.0	38.0	41.0	35.0	41.0
12	39.04193763035959	40.0	38.0	41.0	35.0	41.0
13	38.9588641739657	40.0	38.0	41.0	35.0	41.0
14	38.95284901718767	40.0	38.0	41.0	35.0	41.0
15	38.89187207928504	40.0	38.0	41.0	35.0	41.0
16	38.948889860168705	40.0	38.0	41.0	35.0	41.0
17	39.057637968716996	40.0	38.0	41.0	35.0	41.0
18	39.08651844588262	40.0	39.0	41.0	35.0	41.0
19	39.06947258818249	40.0	39.0	41.0	35.0	41.0
20	39.09636502996621	40.0	39.0	41.0	35.0	41.0
21	39.121530619653605	40.0	38.0	41.0	35.0	41.0
22	39.137184806176734	40.0	38.0	41.0	35.0	41.0
23	39.09411147972991	40.0	38.0	41.0	35.0	41.0
24	39.03089445039405	40.0	38.0	41.0	35.0	41.0
25	39.03636330694391	40.0	38.0	41.0	35.0	41.0
26	38.995785299461694	40.0	38.0	41.0	35.0	41.0
27	38.973543036909646	40.0	38.0	41.0	35.0	41.0
28	38.9215531039561	40.0	38.0	41.0	35.0	41.0
29	38.885600187566766	40.0	38.0	41.0	35.0	41.0
30	38.88556593988151	40.0	38.0	41.0	35.0	41.0
31	38.80141623502805	40.0	38.0	41.0	35.0	41.0
32	38.5961978520027	40.0	38.0	41.0	35.0	41.0
33	38.48336402592	40.0	38.0	41.0	34.0	41.0
34	38.52054237004847	40.0	37.0	41.0	34.0	41.0
35	38.547380959047416	40.0	37.0	41.0	34.0	41.0
36	38.555874007477435	40.0	37.0	41.0	35.0	41.0
37	38.50365719651146	40.0	37.0	41.0	35.0	41.0
38	38.431345021367704	40.0	37.0	41.0	34.0	41.0
39	38.391490008872765	40.0	37.0	41.0	34.0	41.0
40	38.29967048612491	40.0	37.0	41.0	34.0	41.0
41	38.22928467322179	40.0	36.0	41.0	34.0	41.0
42	38.16002347691288	40.0	36.0	41.0	34.0	41.0
43	38.056666920512725	39.0	36.0	41.0	34.0	41.0
44	37.91906190680515	39.0	36.0	41.0	34.0	41.0
45	37.7194408814494	39.0	35.0	41.0	34.0	41.0
46	37.449172935092186	39.0	35.0	41.0	33.0	41.0
47	37.23513459289478	39.0	35.0	41.0	33.0	41.0
48	37.1416812283351	38.0	35.0	40.0	32.0	41.0
49	37.27320367919996	38.0	35.0	40.0	33.0	41.0
50	36.99628060632652	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	6.0
20	29.0
21	80.0
22	169.0
23	356.0
24	741.0
25	1567.0
26	3216.0
27	7206.0
28	16429.0
29	35074.0
30	71696.0
31	134644.0
32	237731.0
33	415007.0
34	763868.0
35	744238.0
36	1171199.0
37	1887202.0
38	2794009.0
39	4637645.0
40	6470396.0
41	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.510128651718766	19.06228548421164	28.42758586406959
2	27.162695580644737	22.21208371561147	30.048773464727002	20.576447239016794
3	25.41171561476925	21.04000760705988	28.354557676695002	25.19371910147587
4	22.371078073846235	23.135306039774527	31.45237192582374	23.041243960555498
5	19.477590113132326	24.8302179727928	30.762420051615795	24.929771862459077
6	21.430668703466573	27.169950958390537	29.573125957070438	21.826254381072445
7	26.046537962694057	26.176526279834196	24.609632831476397	23.167302925995354
8	23.924672574651495	23.712446329799874	27.72120496818566	24.641676127362974
9	23.196834943301827	25.440438041239837	28.865981880016367	22.496745135441966
10	23.440563037939594	23.408107211690783	27.642370415962613	25.50895933440701
11	25.0106329700866	24.07012562374591	27.392438896002748	23.52680251016475
12	23.81483120907699	26.553377922365083	26.464163074902313	23.167627793655612
13	24.9680082704087	25.15378100578203	25.367384070717602	24.510826653091666
14	22.011037043576405	26.22813382814975	26.096309750897667	25.664519377376177
15	22.38289191209473	27.737221459499416	28.696875371277326	21.183011257128527
16	22.93664173058137	29.210970708694163	25.03126980145739	22.821117759267082
17	23.374388011207625	32.177610615891325	22.58898176780811	21.859019605092936
18	24.03715929117383	26.92674368333509	23.373810468700494	25.66228655679058
19	26.3744789741525	24.551569182991866	24.52047986356796	24.553471979287675
20	26.394888913824055	23.317551640550743	27.476182559536383	22.811376886088816
21	23.20779793767821	27.504368696536062	25.817547515247124	23.4702858505386
22	26.380022350895025	26.537820371079313	23.512693971776322	23.56946330624934
23	28.25987680193262	25.013721791173833	25.682763532646003	21.043637874247544
24	23.027852193671457	29.126876394352625	25.17032862993716	22.674942782038755
25	23.869381031866347	26.267402744061602	26.96623280755514	22.89698341651691
26	23.054709724264804	29.42328276846389	26.57943435466585	20.942573152605462
27	25.21925713334867	26.650944897127776	25.972170277092278	22.157627692431277
28	24.666741377596868	26.21523373338975	25.60307235945784	23.514952529555547
29	25.34696189214774	28.109698311672492	24.392006110148678	22.151333686031087
30	27.299512823268845	27.730675614212792	24.17170160279609	20.798109959722275
31	24.9414195830913	26.779567236101105	26.234822808135664	22.044190372671938
32	21.936198551144827	27.324257497198403	28.949094243978095	21.790449707678675
33	22.336868323668522	25.568940638085692	30.291853886819432	21.80233715142635
34	22.678494623655915	26.37657808234442	26.452509889130315	24.49241740486935
35	20.909244698646166	26.293693412448533	27.713132903018266	25.083928985887034
36	23.154857333620026	25.002590292956384	28.022291273015533	23.820261100408057
37	21.73129539448825	29.281829064800995	24.70456359028896	24.28231195042179
38	23.861783166572252	26.90972443530713	25.857630243694	23.370862154426614
39	22.935836910212732	27.768568306328945	27.03486567975788	22.26072910370044
40	21.25133847727515	26.661418149851634	27.485359643049158	24.601883729824053
41	20.72989137403444	27.608900750361343	27.352225138955855	24.308982736648364
42	21.079026290272864	28.852248681433068	25.852466215481112	24.216258812812956
43	21.723655106286497	25.923653490902502	28.867390820234302	23.4853005825767
44	22.8734595626952	28.51818115483352	24.45054686084384	24.157812421627437
45	20.960055152818367	27.076637873898772	28.60535364983071	23.357953323452147
46	20.6361423473191	29.90692950760226	24.82658537791929	24.630342767159352
47	22.013643682101932	26.81971279876903	28.431505572255155	22.735137946873884
48	22.157020164691584	28.22857087865442	26.53082272526342	23.08358623139058
49	20.418003065105307	28.919308821437482	25.811850309060315	24.850837804396896
50	20.20484605645532	30.489698856501917	27.16102147412348	22.144433612919283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143627.0
1	146948.0
2	132091.5
3	112218.5
4	104603.5
5	71111.0
6	32253.0
7	23442.5
8	28027.0
9	31490.5
10	32555.0
11	32043.0
12	30780.0
13	29837.5
14	33703.0
15	38785.5
16	42556.0
17	48305.0
18	54921.0
19	59257.0
20	61582.5
21	70678.5
22	84651.5
23	108422.0
24	135189.0
25	163164.0
26	199110.5
27	240716.0
28	281243.5
29	328063.0
30	405029.0
31	528774.5
32	656963.5
33	680017.0
34	655391.0
35	673388.5
36	707203.0
37	778040.5
38	850812.0
39	933337.5
40	1045856.0
41	1144655.0
42	1240157.5
43	1305581.0
44	1342380.0
45	1378163.0
46	1441260.5
47	1543343.5
48	1675979.5
49	1797907.0
50	1978280.5
51	2076194.0
52	1929268.5
53	1717766.0
54	1547056.0
55	1381456.0
56	1249575.0
57	1164835.0
58	1062486.0
59	929163.0
60	773379.5
61	615454.5
62	487102.0
63	396848.0
64	332148.0
65	290126.0
66	215929.5
67	141118.0
68	99148.5
69	70164.5
70	51294.5
71	37114.0
72	27433.5
73	18191.0
74	11851.5
75	8405.5
76	5950.0
77	4215.0
78	2574.5
79	1430.5
80	1087.5
81	980.0
82	1023.5
83	994.5
84	858.0
85	650.0
86	285.5
87	65.0
88	28.5
89	26.0
90	17.0
91	6.5
92	4.5
93	4.0
94	17.5
95	96.0
96	161.5
97	162.0
98	81.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	74797.0
25	75304.0
26	71541.0
27	92894.0
28	102333.0
29	89273.0
30	177046.0
31	315353.0
32	379306.0
33	65665.0
34	55599.0
35	67232.0
36	78695.0
37	96773.0
38	55289.0
39	57759.0
40	60340.0
41	59350.0
42	84623.0
43	147798.0
44	553935.0
45	1329120.0
46	2183741.0
47	2104097.0
48	3563029.0
49	3228351.0
50	4223269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.786916213152491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.91748416289532	6.744896431235203
2	12.700143100262512	2.4859047283463185
3	5.215581465015114	1.531333764029221
4	2.7077752652503135	1.0600307858020632
5	1.6705425651902122	0.817473005801072
6	1.075196787879553	0.6313716525376727
7	0.7834204027542144	0.5367088889000773
8	0.6093043011663674	0.4770568115062936
9	0.5019212393621393	0.4421035003715023
>10	3.8004649950260676	7.370463594502137
>50	0.4672074011131918	3.2503772400309416
>100	1.1929672391510837	32.72329901691472
>500	0.2823677473499842	17.595151483160564
>1k	0.06525561331845765	11.389431296886034
>5k	0.006098655450323146	3.989385579615613
>10k+	0.004269058815226202	8.95501222036065
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	61939	0.31939647633065793	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	51486	0.26549422787517163	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	46708	0.24085585199070653	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA	39698	0.20470787899989437	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	35360	0.18233842010771995	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	34040	0.17553166913084803	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	28431	0.1466081341086704	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	26711	0.1377387313206252	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	24282	0.12521327819727532	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	23764	0.12254214410180589	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	22445	0.11574054975446192	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	21781	0.11231654774791426	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA	21242	0.10953712443235822	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	19618	0.10116275807899461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05343815179797233	0.0	0.0	0.0	0.0
2	0.0820883854551695	0.0	0.0	0.0	0.0
3	0.10396280791266237	0.0	0.0	0.0	0.0
4	0.14270456555602493	0.0	0.0	0.0	0.0
5	0.1648419761194424	0.0	0.0	0.0	0.0
6	0.24094351469268138	0.0	0.0	0.0	0.0
7	0.2717285929744429	0.0	0.0	0.0	0.0
8	0.3080209515920372	0.0	0.0	0.0	0.0
9	0.3506095548632379	0.0	0.0	0.0	0.0
10	0.3802808011669659	0.0	0.0	0.0	0.0
11	0.41672785867039813	0.0	0.0	0.0	0.0
12	0.45219515656352305	0.0	0.0	0.0	0.0
13	0.48500163362023446	0.0	0.0	0.0	0.0
14	0.5195252683097475	0.0	0.0	0.0	0.0
15	0.5585248574295069	0.0	0.0	0.0	5.156629527933256E-6
16	0.5942087337628049	0.0	0.0	0.0	5.156629527933256E-6
17	0.6414434602386736	0.0	0.0	0.0	5.156629527933256E-6
18	0.6716458393837786	0.0	0.0	0.0	5.156629527933256E-6
19	0.7037252316770515	0.0	0.0	0.0	5.156629527933256E-6
20	0.7441016408807688	0.0	0.0	0.0	5.156629527933256E-6
21	0.7740100921427817	0.0	0.0	0.0	5.156629527933256E-6
22	0.8025004702846129	0.0	0.0	0.0	5.156629527933256E-6
23	0.8314446318249024	0.0	0.0	0.0	5.156629527933256E-6
24	0.8621265775161052	0.0	0.0	0.0	5.156629527933256E-6
25	0.8903178711453162	0.0	0.0	0.0	5.156629527933256E-6
26	0.922479769511036	0.0	0.0	0.0	5.156629527933256E-6
27	0.9513568948674622	0.0	0.0	0.0	5.156629527933256E-6
28	0.9803268395553912	0.0	0.0	0.0	5.156629527933256E-6
29	1.012885798394762	0.0	0.0	0.0	5.156629527933256E-6
30	1.0431088040579786	0.0	0.0	0.0	5.156629527933256E-6
31	1.0723829898880557	0.0	0.0	0.0	5.156629527933256E-6
32	1.1026369353284402	0.0	0.0	0.0	5.156629527933256E-6
33	1.132168952634914	0.0	0.0	0.0	5.156629527933256E-6
34	1.1626704162926391	0.0	0.0	0.0	5.156629527933256E-6
35	1.1917641200892386	0.0	0.0	0.0	5.156629527933256E-6
36	1.2152577242185025	0.0	0.0	0.0	5.156629527933256E-6
37	1.2329707466469533	0.0	0.0	0.0	5.156629527933256E-6
38	1.2477341769854262	0.0	0.0	0.0	5.156629527933256E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGT	155	0.0	113.33495	44
CTATCAT	18870	0.0	99.415054	44
GAATACA	14620	0.0	94.29144	44
AACGACA	3630	0.0	88.094345	43
CCGATAC	120	0.0	87.22925	43
GCGTTAC	190	0.0	85.392845	43
ACGAACA	905	0.0	72.5357	44
GACGAAT	265	0.0	69.77922	44
TGCACCG	625	0.0	69.528015	44
GCGTTAG	115	2.8740033E-10	64.31824	44
CGGGCAC	885	0.0	63.869556	43
TGAATAC	12490	0.0	63.77723	43
CCTATCA	17625	0.0	60.340374	43
CCCTATC	18290	0.0	60.304615	42
GTCTACG	230	0.0	60.29835	44
CCTTAGC	15540	0.0	58.73661	43
CGTCGTT	45	8.540004E-6	58.152832	43
TCGCATT	175	0.0	58.116123	44
ACTTCTA	10250	0.0	57.278526	44
CTAACGA	195	0.0	56.896908	44
>>END_MODULE