FastQCFastQC Report
Tue 24 May 2016
ERR841112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7005989
Sequences flagged as poor quality0
Sequence length24-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA698120.9964617415185778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA646250.9224250851664197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA643790.9189138036043163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA550160.7852710017101083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA496020.7079942603392612No Hit
AAAAAAAAAAAAAAAAAAAAAAAA445250.6355276892384502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA321520.4589216454664716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181070.25845030587401724No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA169240.24156475267089342No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA166040.2369972319397019No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA158600.22637774623968152No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA152480.21764236284127764No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA152170.21719988427044348No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA151740.21658612367218963No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA139340.19888698083882234No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC136910.19541851978357375No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA126500.18055980390491622No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116920.16688578871591148No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111810.15959202904828998No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA110320.1574652772078289No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA105370.15039989357676697No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG104600.149300833900824No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA104130.14862997929343025No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA95330.13606929728265346Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAA94990.13558399820496436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92850.13252946871598No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA92660.1322582721725655No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA90050.1285328880761874Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA86010.12276639315305804Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA84900.12118203439942597No Hit
CAAAAAAAAAAAAAAAAAAAAAAA83750.11954058163665401No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA83000.11847006896528098No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA81540.11638613763167484No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA75800.10819314732009998No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA75540.10782203626069067No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA74080.10573810492708452No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73420.10479605377627627No Hit
TAAAAAAAAAAAAAAAAAAAAAAA71670.10229819087640589No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70840.10111349018675309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT1500.0198.8559744
GCCCGAT1250.0182.947544
CTTGCGT550.0180.7781744
ATCGTGT1050.0179.9173144
ACGAACA1250.0174.9932644
CTAGTGG1500.0172.3418444
TAAGTAC650.0168.2627444
ATCTAGC2050.0145.5043844
GAATTCG1400.0142.0399844
TAGGACG1800.0138.0944444
CGACACC451.2150849E-9132.5706644
AACGCTA1950.0132.5706544
AATGCCG850.0128.6715244
CGACCTG2450.0125.8068544
CTATCAT39350.0123.3058744
GTTAGAC1600.0116.8292143
GCCCGTA401.8189894E-12116.8292143
ACGACAC9800.0114.6465744
GACGAAC5050.0114.51575543
TGTACGG1850.0113.6716543
Produced by FastQC (version 0.11.5)