##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841112.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7005989
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.047927708707505	39.0	37.0	39.0	33.0	39.0
2	37.64387140773415	39.0	37.0	39.0	35.0	39.0
3	37.57075938886002	39.0	37.0	39.0	34.0	39.0
4	38.24322147808111	39.0	37.0	40.0	35.0	41.0
5	38.992326850641646	40.0	38.0	41.0	35.0	41.0
6	38.894176111324185	40.0	38.0	41.0	35.0	41.0
7	39.064146832088944	40.0	38.0	41.0	35.0	41.0
8	38.79622862667926	40.0	38.0	41.0	35.0	41.0
9	38.94258911910938	40.0	38.0	41.0	35.0	41.0
10	38.990910062804836	40.0	38.0	41.0	35.0	41.0
11	38.97425802409909	40.0	38.0	41.0	35.0	41.0
12	38.881219482360024	40.0	38.0	41.0	35.0	41.0
13	38.84887843814771	40.0	38.0	41.0	35.0	41.0
14	38.8052884467846	40.0	38.0	41.0	35.0	41.0
15	38.707381498886164	40.0	38.0	41.0	35.0	41.0
16	38.68627384370715	40.0	38.0	41.0	35.0	41.0
17	38.7172733214397	40.0	38.0	41.0	35.0	41.0
18	38.658231835648046	40.0	38.0	41.0	35.0	41.0
19	38.61725846272382	40.0	38.0	41.0	35.0	41.0
20	38.64165644564957	40.0	37.0	41.0	35.0	41.0
21	38.611929450645725	40.0	37.0	41.0	35.0	41.0
22	38.50398780243589	40.0	37.0	41.0	35.0	41.0
23	38.14220576138501	40.0	35.0	41.0	34.0	41.0
24	37.968780567597236	40.0	35.0	41.0	33.0	41.0
25	37.93636600546394	40.0	35.0	41.0	33.0	41.0
26	37.90354471611729	40.0	35.0	41.0	33.0	41.0
27	37.922994654011816	40.0	35.0	41.0	33.0	41.0
28	38.01125605373561	40.0	36.0	41.0	33.0	41.0
29	38.11341109258504	40.0	37.0	41.0	33.0	41.0
30	38.249609978401026	40.0	37.0	41.0	34.0	41.0
31	38.24136016337769	40.0	37.0	41.0	34.0	41.0
32	38.132631705627254	40.0	36.0	41.0	34.0	41.0
33	37.98277071829741	40.0	35.0	41.0	34.0	41.0
34	37.96617425208539	40.0	35.0	41.0	34.0	41.0
35	37.96183055089995	40.0	35.0	41.0	34.0	41.0
36	37.93736963816088	40.0	35.0	41.0	34.0	41.0
37	37.933307120969125	40.0	35.0	41.0	34.0	41.0
38	37.885686118858686	40.0	35.0	41.0	34.0	41.0
39	37.82789601586776	39.0	35.0	41.0	34.0	41.0
40	37.76100087143032	39.0	35.0	41.0	33.0	41.0
41	37.678698833104754	39.0	35.0	41.0	33.0	41.0
42	37.595724446665535	39.0	35.0	41.0	33.0	41.0
43	37.54894415682717	39.0	35.0	41.0	33.0	41.0
44	37.465258745557456	39.0	35.0	41.0	33.0	41.0
45	37.277226733074315	39.0	35.0	41.0	33.0	41.0
46	37.04272889776736	39.0	35.0	41.0	33.0	41.0
47	36.77550381199491	38.0	35.0	41.0	32.0	41.0
48	36.68211368925623	38.0	35.0	40.0	31.0	41.0
49	36.77416810985342	38.0	35.0	40.0	32.0	41.0
50	36.4395667601828	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	8.0
19	19.0
20	115.0
21	313.0
22	686.0
23	1231.0
24	2408.0
25	3372.0
26	5667.0
27	9924.0
28	17307.0
29	29367.0
30	49811.0
31	84073.0
32	146419.0
33	282116.0
34	420751.0
35	326858.0
36	618344.0
37	874468.0
38	938758.0
39	1207887.0
40	1986086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	56.1273504711469	16.68278382966345	27.189865699189653
2	31.650406530755333	30.08100640751791	24.071633569507462	14.1969534922193
3	19.37886285576526	26.781871909875964	32.557930079536234	21.28133515482254
4	26.962845645347144	32.57684247006382	24.252878501522055	16.207433383066974
5	19.029504613838245	29.189983027378435	24.179783896320707	27.600728462462616
6	16.785167661553565	42.03790499813802	26.314600265572786	14.86232707473563
7	32.410613262453026	31.167448307440964	19.064788711486703	17.357149718619315
8	19.31735833441931	31.901905641016565	28.519456710537227	20.261279314026897
9	26.283212834048125	33.43939021314478	24.617694946423697	15.659702006383396
10	21.086173558080095	30.276096636748928	23.074943451952322	25.56278635321865
11	18.64264988140861	30.023940945382588	33.50590473379276	17.827504439416046
12	21.208811489712588	39.68603147963835	21.44466684146949	17.660490189179573
13	17.921252802423755	28.64677635091919	31.71660703435304	21.715363812304016
14	25.75470786494241	31.5183052671079	25.340847666189596	17.386139201760095
15	16.1611444151568	33.08573564702999	25.15867495652648	25.594444981286724
16	20.37037169199095	33.11708025804779	30.272556808182255	16.239991241779
17	21.87255789296843	33.791874352072206	27.713060925445358	16.622506829514005
18	28.03985276025983	31.85083504984093	20.712835832314326	19.39647635758492
19	27.74122254545361	30.22068690087866	24.355462162444162	17.682628391223567
20	29.667702875354212	30.262122878011944	22.907658005172433	17.16251624146141
21	30.41250849808642	32.161026801497975	20.433603307113383	16.992861393302217
22	28.007808747630065	34.71070251466281	20.451759202019872	16.829729535687253
23	28.48427252740477	34.56302886002248	19.883902187114483	17.068796425458277
24	23.84890983985273	39.06768908715101	20.08036267256486	17.003038400431404
25	23.026938907878982	38.83078451045372	21.405537192834633	16.736739388832667
26	22.97130594068369	40.40508307801908	21.11112664789006	15.512484333407173
27	21.43953659596473	39.865130692636825	22.30537203139295	16.389960680005494
28	22.27266692809033	39.526306459645724	20.655395776754425	17.545630835509517
29	19.980147576068646	40.32974552759237	21.268671302038634	18.42143559430035
30	21.85410612881014	40.70298729790409	21.283772634309532	16.15913393897624
31	20.419908757380696	38.82702525707408	22.119484226883337	18.633581758661887
32	20.061292832482692	38.71224878839264	23.73445009865856	17.492008280466106
33	18.06076400141704	37.1236758544509	25.76178772037942	19.05377242375264
34	20.21425590947542	36.61705591485951	24.321302067388846	18.84738610827622
35	19.06579167029648	37.8529177285681	24.012409973375963	19.068880627759455
36	18.50541677421155	38.82534827102706	23.869313782254746	18.799921172506647
37	17.711997170046313	39.61763371066633	23.16558296712861	19.504786152158744
38	19.045084148166225	38.10337513087814	23.33048155045769	19.521059170497942
39	18.204136038698035	39.647158711223526	23.369558088708406	18.77914716137003
40	17.08643711272984	40.56697189913667	24.060804648008855	18.28578634012464
41	17.907107044090203	40.995552757605054	22.53750927445646	18.55983092384828
42	18.472683571354377	39.674903519215164	22.568331930218587	19.28408097921187
43	17.017744370631593	40.18372623328136	23.020204444970542	19.7783249511165
44	18.900013239173987	39.157069821611	22.314805149795934	19.62811178941908
45	16.615665412178238	38.377949360945614	24.935156836833045	20.071228390043107
46	18.4425531752495	38.54013769029627	23.348557105683906	19.668752028770324
47	19.6863492035015	38.193928964857186	24.062973353536396	18.056748478104918
48	18.382083580745878	37.25631859712299	24.088843450112055	20.27275437201908
49	18.555409340043287	38.59527081173693	23.161242032001077	19.6880778162187
50	18.442948918622992	36.79640095203716	25.262761357117284	19.497888772222563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	553900.0
1	546308.0
2	375260.0
3	210564.5
4	205125.0
5	153307.0
6	68413.5
7	32080.5
8	32305.0
9	28708.5
10	23482.0
11	19959.5
12	17610.5
13	16381.0
14	18764.0
15	22684.0
16	26371.0
17	31235.5
18	34325.0
19	35207.0
20	40592.0
21	58256.0
22	78579.5
23	102012.0
24	134812.5
25	170668.5
26	211349.5
27	246463.5
28	261436.0
29	266253.0
30	271861.0
31	281144.0
32	294014.0
33	298862.5
34	302318.5
35	309931.5
36	311231.0
37	317251.5
38	334655.5
39	341987.0
40	348416.0
41	374205.0
42	402196.5
43	414602.0
44	425730.5
45	432554.5
46	438361.0
47	442812.5
48	440581.0
49	442531.5
50	457514.0
51	471020.0
52	466402.5
53	441934.5
54	400228.5
55	354023.0
56	323652.5
57	306306.0
58	274133.0
59	226512.5
60	180470.5
61	141794.5
62	116275.5
63	102259.5
64	88207.0
65	78782.5
66	68433.5
67	57044.5
68	45960.5
69	34283.0
70	23283.5
71	15114.0
72	8881.5
73	4946.5
74	2941.5
75	1871.5
76	1298.5
77	705.5
78	235.5
79	140.0
80	99.0
81	110.0
82	105.0
83	77.0
84	62.5
85	41.5
86	22.5
87	15.5
88	7.0
89	5.0
90	7.5
91	8.0
92	5.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	98879.0
25	113195.0
26	131910.0
27	146400.0
28	145602.0
29	128041.0
30	118727.0
31	140480.0
32	150881.0
33	37028.0
34	32384.0
35	28422.0
36	29353.0
37	33381.0
38	29676.0
39	34756.0
40	47249.0
41	52829.0
42	66310.0
43	85167.0
44	183224.0
45	455568.0
46	703274.0
47	672005.0
48	1071387.0
49	934619.0
50	1335242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.51594115308012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66812161459904	7.431418082978329
2	11.957606917114418	2.5149098134407804
3	4.711676621690065	1.4864314225810822
4	2.46677939681898	1.0376202789831548
5	1.4996221653223671	0.7884969221192301
6	1.0520318948019363	0.6637863298140322
7	0.7562798003158858	0.5567095712769528
8	0.5987204505141114	0.5036887219801611
9	0.46035482210731815	0.43569577969355017
>10	3.9902805278953393	8.493859830054188
>50	0.5694710021720583	4.235344492509485
>100	1.0028464010161917	27.237131643469407
>500	0.20350045363771732	13.70150019567814
>1k	0.050454657926706774	10.602392849713233
>5k	0.006727287723560902	5.1810709907219685
>10k+	0.005525986344353599	15.129943074986297
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	69812	0.9964617415185778	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	64625	0.9224250851664197	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	64379	0.9189138036043163	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	55016	0.7852710017101083	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49602	0.7079942603392612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	44525	0.6355276892384502	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32152	0.4589216454664716	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18107	0.25845030587401724	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA	16924	0.24156475267089342	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	16604	0.2369972319397019	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA	15860	0.22637774623968152	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15248	0.21764236284127764	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15217	0.21719988427044348	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	15174	0.21658612367218963	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA	13934	0.19888698083882234	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	13691	0.19541851978357375	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	12650	0.18055980390491622	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11692	0.16688578871591148	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11181	0.15959202904828998	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	11032	0.1574652772078289	No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	10537	0.15039989357676697	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	10460	0.149300833900824	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	10413	0.14862997929343025	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	9533	0.13606929728265346	Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAA	9499	0.13558399820496436	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9285	0.13252946871598	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA	9266	0.1322582721725655	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	9005	0.1285328880761874	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	8601	0.12276639315305804	Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA	8490	0.12118203439942597	No Hit
CAAAAAAAAAAAAAAAAAAAAAAA	8375	0.11954058163665401	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	8300	0.11847006896528098	No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	8154	0.11638613763167484	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7580	0.10819314732009998	No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	7554	0.10782203626069067	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	7408	0.10573810492708452	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7342	0.10479605377627627	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	7167	0.10229819087640589	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7084	0.10111349018675309	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.35542448039812796	0.0	0.0	0.0	0.0
2	0.524708217497915	0.0	0.0	0.0	0.0
3	0.67076896638005	0.0	0.0	0.0	0.0
4	0.846019027434956	0.0	0.0	0.0	0.0
5	0.9354710662548856	0.0	0.0	0.0	0.0
6	1.2118631645011146	0.0	0.0	0.0	0.0
7	1.325794259739774	0.0	0.0	0.0	0.0
8	1.467558684434132	0.0	0.0	0.0	0.0
9	1.6215555005867124	0.0	0.0	0.0	0.0
10	1.7200997603621702	0.0	0.0	0.0	0.0
11	1.8372423936149487	0.0	0.0	0.0	0.0
12	2.001330575883005	0.0	0.0	0.0	0.0
13	2.1045850914125044	0.0	0.0	0.0	0.0
14	2.2218561861858475	0.0	0.0	0.0	0.0
15	2.3139773699330672	0.0	0.0	0.0	0.0
16	2.392981205080396	0.0	0.0	0.0	0.0
17	2.503272557236387	0.0	0.0	0.0	0.0
18	2.5956363905224515	0.0	0.0	0.0	0.0
19	2.6664615088604906	0.0	0.0	0.0	0.0
20	2.7422823529982705	0.0	0.0	0.0	0.0
21	2.8311491782245164	0.0	0.0	0.0	0.0
22	2.9227850628940466	0.0	0.0	0.0	0.0
23	3.001089496429412	0.0	0.0	0.0	0.0
24	3.092582646076093	0.0	0.0	0.0	0.0
25	3.1834334881199498	0.0	0.0	0.0	0.0
26	3.2784664663333043	0.0	0.0	0.0	0.0
27	3.3618665401844052	0.0	0.0	0.0	0.0
28	3.443368238231604	0.0	0.0	0.0	0.0
29	3.5233712185388817	0.0	0.0	0.0	0.0
30	3.5980930030007183	0.0	0.0	0.0	0.0
31	3.6650928227263844	0.0	0.0	0.0	0.0
32	3.72968042056589	0.0	0.0	0.0	0.0
33	3.791969984537515	0.0	0.0	0.0	0.0
34	3.850277241371632	0.0	0.0	0.0	0.0
35	3.900077490843905	0.0	0.0	0.0	0.0
36	3.939072699086453	0.0	0.0	0.0	0.0
37	3.9615820121898566	0.0	0.0	0.0	0.0
38	3.981293718845405	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGCT	150	0.0	198.85597	44
GCCCGAT	125	0.0	182.9475	44
CTTGCGT	55	0.0	180.77817	44
ATCGTGT	105	0.0	179.91731	44
ACGAACA	125	0.0	174.99326	44
CTAGTGG	150	0.0	172.34184	44
TAAGTAC	65	0.0	168.26274	44
ATCTAGC	205	0.0	145.50438	44
GAATTCG	140	0.0	142.03998	44
TAGGACG	180	0.0	138.09444	44
CGACACC	45	1.2150849E-9	132.57066	44
AACGCTA	195	0.0	132.57065	44
AATGCCG	85	0.0	128.67152	44
CGACCTG	245	0.0	125.80685	44
CTATCAT	3935	0.0	123.30587	44
GTTAGAC	160	0.0	116.82921	43
GCCCGTA	40	1.8189894E-12	116.82921	43
ACGACAC	980	0.0	114.64657	44
GACGAAC	505	0.0	114.515755	43
TGTACGG	185	0.0	113.67165	43
>>END_MODULE