FastQCFastQC Report
Tue 24 May 2016
ERR841111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167101
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA184750.4433537848014723No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG155430.37299311919725486No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT95590.2293920881687293No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC89080.21376971664473693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA87410.20976213439511063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA82900.19893926257126957No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC80690.19363581540260244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA79700.19126006305102758No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG72650.17434182660799438No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.16827045948730304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70110.1682464619888023No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.16779050951728794No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT65910.15816751261848463No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG65860.1580475251259809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA59400.14254514109449232No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG58920.141393261166456No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.1336660666492125No Hit
AAAAAAAAAAAAAAAAAAAAAAAA54340.13040240685310964No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT53430.1282186344895408No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA48620.11667583771067705No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA42840.10280528357723989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC400.0232.6967344
TACGAAC900.0232.6967344
CGAATAC800.0232.6967344
CAAGCGC1000.0232.6967344
TATCCAC450.0232.6967344
ACGCGGG750.0232.6967244
CGCAGGC900.0219.7691344
ATAACGG550.0211.5424744
GTCGCCC450.0206.8415444
ATAACCG650.0196.8972344
AATTCGT256.138471E-7186.1573844
ACAAATT2000.0174.5225544
ACCGTCA205.876724E-5174.5225544
CTCGCCA1400.0174.5225444
TCGAGCT1200.0164.8268444
GATCCTT150.005793926155.1311344
TCACGTC150.005793926155.1311344
TCCATTG1750.0146.2665144
TCGAGTT551.6425474E-9126.92547644
GTAAAGG750.0124.1049144
Produced by FastQC (version 0.11.5)