##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841111.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4167101
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.29172007109979	39.0	37.0	39.0	34.0	39.0
2	37.86008858436597	39.0	38.0	39.0	35.0	39.0
3	37.81511535237567	39.0	37.0	39.0	35.0	39.0
4	38.45640746408594	39.0	38.0	40.0	35.0	41.0
5	39.123628392976315	40.0	38.0	41.0	35.0	41.0
6	39.13839117410401	40.0	38.0	41.0	35.0	41.0
7	39.22631656876087	40.0	39.0	41.0	35.0	41.0
8	38.94240048417353	40.0	38.0	41.0	35.0	41.0
9	39.09297494829139	40.0	38.0	41.0	35.0	41.0
10	39.10502673201346	40.0	39.0	41.0	35.0	41.0
11	38.97706847038265	40.0	38.0	41.0	35.0	41.0
12	38.98639485819998	40.0	38.0	41.0	35.0	41.0
13	38.906817953296546	40.0	38.0	41.0	35.0	41.0
14	38.87635552869969	40.0	38.0	41.0	35.0	41.0
15	38.64721541426522	40.0	38.0	41.0	35.0	41.0
16	38.851755932961545	40.0	38.0	41.0	35.0	41.0
17	38.718050990364766	40.0	38.0	41.0	35.0	41.0
18	38.803655826916604	40.0	38.0	41.0	35.0	41.0
19	38.847275599991455	40.0	38.0	41.0	35.0	41.0
20	38.915226196821244	40.0	38.0	41.0	35.0	41.0
21	38.89791320152787	40.0	38.0	41.0	35.0	41.0
22	38.884589310410284	40.0	38.0	41.0	35.0	41.0
23	38.79946730352828	40.0	38.0	41.0	35.0	41.0
24	38.741370559532875	40.0	38.0	41.0	35.0	41.0
25	38.72032880408949	40.0	38.0	41.0	35.0	41.0
26	38.71503629129851	40.0	38.0	41.0	35.0	41.0
27	38.669194855973366	40.0	38.0	41.0	35.0	41.0
28	38.6678776143231	40.0	38.0	41.0	35.0	41.0
29	38.64236123438137	40.0	38.0	41.0	35.0	41.0
30	38.68887466939894	40.0	38.0	41.0	35.0	41.0
31	38.54554472905165	40.0	37.0	41.0	35.0	41.0
32	38.43663434733149	40.0	37.0	41.0	34.0	41.0
33	38.31068628976469	40.0	37.0	41.0	34.0	41.0
34	38.37410915956617	40.0	37.0	41.0	34.0	41.0
35	38.38553060115806	40.0	37.0	41.0	34.0	41.0
36	38.416749216069	40.0	37.0	41.0	34.0	41.0
37	38.382005537564176	40.0	37.0	41.0	34.0	41.0
38	38.2411993231358	40.0	36.0	41.0	34.0	41.0
39	38.224580385441385	40.0	36.0	41.0	34.0	41.0
40	38.14449231431376	40.0	36.0	41.0	34.0	41.0
41	38.072612662137644	39.0	36.0	41.0	34.0	41.0
42	37.971085455291664	39.0	35.0	41.0	34.0	41.0
43	37.87719032789706	39.0	36.0	41.0	34.0	41.0
44	37.73450470133833	39.0	35.0	41.0	34.0	41.0
45	37.538027817626144	39.0	35.0	41.0	33.0	41.0
46	37.279669862917494	39.0	35.0	41.0	33.0	41.0
47	37.0239148715742	38.0	35.0	40.0	32.0	41.0
48	36.971709819034004	38.0	35.0	40.0	32.0	41.0
49	36.853379925260256	38.0	35.0	40.0	32.0	41.0
50	36.744736663191766	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	47.0
22	100.0
23	176.0
24	388.0
25	649.0
26	1369.0
27	2705.0
28	5416.0
29	10662.0
30	20500.0
31	36166.0
32	62782.0
33	115689.0
34	199291.0
35	185349.0
36	290814.0
37	431205.0
38	590145.0
39	905035.0
40	1308601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.621666477486386	20.55683315571185	25.821500366801764
2	32.80155676572274	23.035678760845972	25.492734637341403	18.670029836089885
3	24.713271888538337	20.419111511816006	30.92111278320348	23.946503816442174
4	26.228089983900077	23.080674070534887	30.299889539514403	20.391346406050634
5	18.992748195928055	24.425997833985786	26.87539850845948	29.70585546162668
6	20.933641877170725	29.591939336243588	29.26950894638743	20.20490984019826
7	32.0292452714729	23.470225463697663	24.20812454509742	20.292404719732016
8	19.535811587000172	21.616226724526236	34.25878086468266	24.58918082379093
9	26.43917198071273	24.21885142692726	28.94451082419169	20.397465768168328
10	26.01052866249222	20.515197495813037	27.484982965375686	25.989290876319053
11	20.59364531841201	22.299243526854763	34.534440129960856	22.57267102477238
12	24.221971101732358	21.79625595827891	27.48659079777524	26.495182142213498
13	22.561464192972526	22.219955791808264	33.82627394920353	21.392306066015678
14	28.045420545362354	26.399720093177486	24.075394380889737	21.479464980570427
15	20.08633820010602	25.47771220327993	33.73167581011355	20.704273786500497
16	30.65490853233459	26.919097953229354	23.0018182904614	19.42417522397465
17	23.568207250076252	30.642765798093208	26.395904490915868	19.393122460914675
18	27.110334018781884	27.24553592533514	24.254583702194886	21.38954635368809
19	29.07064167631166	24.294971492171655	26.304522016624986	20.329864814891696
20	29.015399434762923	24.475864635870355	25.52292349045536	20.985812438911367
21	25.784760196597105	29.593211203664133	23.2682145213183	21.353814078420466
22	25.903739794163855	28.07368959859624	24.056364364578638	21.966206242661265
23	27.001601353074957	26.621456979324478	26.391992898660245	19.984948768940324
24	23.773001902281706	27.984250921683923	26.114917780970508	22.12782939506386
25	21.531707039301008	29.54027459214366	28.017258643678307	20.91075972487703
26	23.589526102327408	31.235181063222527	25.50676606526292	19.668526769187146
27	23.89777927116107	26.53083666799035	28.55276903809146	21.018615022757125
28	25.224811406191005	27.402251278945442	26.174867573637727	21.198069741225822
29	23.41767956541784	32.116845222682144	25.667949615202335	18.797525596697685
30	25.95196719667064	29.650346466655474	24.880260693613714	19.517425643060175
31	24.672470356426228	29.209751797766653	26.272781852088645	19.844995993718477
32	23.50680926943841	29.12541528989914	27.01966146690787	20.348113973754582
33	22.249957420323437	27.923857024805553	27.951008139552	21.875177415319012
34	22.25115866014642	29.42679981710646	25.027217557779892	23.29482396496723
35	20.20484689483378	28.72173417604113	28.438261053914747	22.635157875210346
36	21.647034901763107	24.85661421652411	29.96758882466507	23.528762057047715
37	20.72240284923511	30.097319266893262	24.363303152752678	24.81697473111895
38	22.85966767660729	28.26997144327515	25.696226862192034	23.174134017925525
39	21.825423457281907	29.488842030516636	27.571052494847503	21.114682017353957
40	20.16407144107025	28.470296830625802	29.94904286225107	21.416588866052884
41	21.591584960384928	31.233194609391614	26.32410305513847	20.85111737508499
42	19.929913950363265	31.57688121196981	26.254376946548753	22.23882789111818
43	20.092401367819075	26.540525319092318	27.959629310187438	25.407444002901165
44	20.457508795182306	29.76487578578075	26.261951791192995	23.515663627843953
45	20.804590532027692	26.348853164766272	29.5842441244004	23.262312178805633
46	19.31456502031048	32.29928991088531	27.176533932626356	21.20961113617786
47	23.229237850727234	28.44917314081633	28.150223311249324	20.171365697207115
48	23.774111342085817	29.53965436668808	25.523272811821606	21.162961479404498
49	20.147782663508103	30.924065852633586	25.384469563903277	23.543681919955034
50	20.798962063182707	29.521710556134707	26.66681774917282	23.01250963150977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68819.0
1	74279.0
2	80030.0
3	78553.0
4	72796.5
5	48506.0
6	19630.0
7	10780.0
8	9435.0
9	7614.0
10	6420.5
11	5609.0
12	5113.5
13	5367.0
14	6399.5
15	6958.0
16	7264.0
17	8449.0
18	9643.0
19	11007.5
20	13567.0
21	17910.5
22	23514.0
23	31010.5
24	37238.5
25	38548.0
26	40646.0
27	46853.5
28	57308.0
29	77087.5
30	93137.5
31	95234.5
32	105604.0
33	126172.0
34	152077.5
35	176733.5
36	176789.5
37	171215.0
38	184359.5
39	209612.0
40	227794.0
41	236556.0
42	259557.5
43	293385.0
44	319434.0
45	331804.0
46	336936.5
47	329850.0
48	345884.0
49	393640.5
50	445469.5
51	444202.5
52	374982.5
53	336079.5
54	312861.5
55	272998.0
56	246435.0
57	234010.5
58	215400.0
59	188199.0
60	157253.5
61	118374.5
62	89763.0
63	67974.5
64	50112.0
65	39507.5
66	24745.5
67	13799.0
68	10642.0
69	8392.5
70	6645.0
71	5895.0
72	5488.0
73	5179.0
74	4894.0
75	4532.0
76	4180.5
77	3360.0
78	2256.5
79	1362.0
80	682.0
81	300.0
82	147.0
83	72.5
84	47.5
85	32.0
86	17.0
87	6.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22627.0
25	25167.0
26	27182.0
27	31944.0
28	32220.0
29	30318.0
30	45100.0
31	80248.0
32	67663.0
33	16636.0
34	14446.0
35	16204.0
36	18518.0
37	29369.0
38	14163.0
39	14948.0
40	16717.0
41	20966.0
42	27567.0
43	37287.0
44	130716.0
45	328754.0
46	427254.0
47	519132.0
48	663253.0
49	802686.0
50	706016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.18861418108019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89087954386314	9.745183018046815
2	11.16864942257087	2.945980163160625
3	4.240615478486064	1.6778352430820838
4	2.222784050125493	1.1726176497985576
5	1.3518609014891732	0.8914585928113979
6	0.8748706340216125	0.6922998750280833
7	0.6486291883188494	0.5988164077927157
8	0.47573780070726396	0.5019457843906979
9	0.3887322736337029	0.46141559790200987
>10	3.0432007930313203	8.097150261201964
>50	0.46204232984724747	4.339697375664245
>100	0.9641720221866722	33.887612509132914
>500	0.2246278904735952	19.153079252873347
>1k	0.036631625215693984	8.761706984270276
>5k	0.005874883289309412	5.522749510528523
>10k+	6.911627399187545E-4	1.5504517743157245
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	18475	0.4433537848014723	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	15543	0.37299311919725486	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	9559	0.2293920881687293	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	8908	0.21376971664473693	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	8741	0.20976213439511063	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	8290	0.19893926257126957	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8069	0.19363581540260244	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	7970	0.19126006305102758	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	7265	0.17434182660799438	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7012	0.16827045948730304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7011	0.1682464619888023	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6992	0.16779050951728794	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6591	0.15816751261848463	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	6586	0.1580475251259809	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5940	0.14254514109449232	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	5892	0.141393261166456	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5570	0.1336660666492125	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	5434	0.13040240685310964	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	5343	0.1282186344895408	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4862	0.11667583771067705	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4284	0.10280528357723989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08082357495054715	0.0	0.0	0.0	0.0
2	0.1195555375307678	0.0	0.0	0.0	0.0
3	0.15483186032687954	0.0	0.0	0.0	0.0
4	0.2164814339753224	0.0	0.0	0.0	0.0
5	0.24254271734714372	0.0	0.0	0.0	0.0
6	0.34232431611328834	0.0	0.0	0.0	0.0
7	0.3791364788134485	0.0	0.0	0.0	0.0
8	0.4302751481185601	0.0	0.0	0.0	0.0
9	0.4855653846643026	0.0	0.0	0.0	0.0
10	0.5204577474844022	0.0	0.0	0.0	0.0
11	0.5664849496088528	0.0	0.0	0.0	0.0
12	0.6072806970601384	0.0	0.0	0.0	0.0
13	0.6550117215781427	0.0	0.0	0.0	0.0
14	0.7036066560421742	0.0	0.0	0.0	0.0
15	0.7524655629897139	0.0	0.0	0.0	0.0
16	0.7933093054380012	0.0	0.0	0.0	0.0
17	0.8392645150669494	0.0	0.0	0.0	0.0
18	0.8876434720444741	0.0	0.0	0.0	0.0
19	0.9281032545167491	0.0	0.0	0.0	0.0
20	0.9709627868390999	0.0	0.0	0.0	0.0
21	1.0104866668698456	0.0	0.0	0.0	0.0
22	1.0530102342131855	0.0	0.0	0.0	0.0
23	1.0925101167454305	0.0	0.0	0.0	0.0
24	1.1338818041607344	0.0	0.0	0.0	0.0
25	1.1750855090865329	0.0	0.0	0.0	0.0
26	1.2180650289013872	0.0	0.0	0.0	0.0
27	1.2662760033894067	0.0	0.0	0.0	0.0
28	1.3061838433961643	0.0	0.0	0.0	0.0
29	1.3491153682140173	0.0	0.0	0.0	0.0
30	1.3866234583706993	0.0	0.0	0.0	0.0
31	1.4250914484674118	0.0	0.0	0.0	0.0
32	1.4616396386840635	0.0	0.0	0.0	0.0
33	1.4988597588587365	0.0	0.0	0.0	0.0
34	1.534352059141355	0.0	0.0	0.0	0.0
35	1.5687884694899403	0.0	0.0	0.0	0.0
36	1.5946577728737557	0.0	0.0	0.0	0.0
37	1.6124159217643153	0.0	0.0	0.0	0.0
38	1.627318368333285	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	40	0.0	232.69673	44
TACGAAC	90	0.0	232.69673	44
CGAATAC	80	0.0	232.69673	44
CAAGCGC	100	0.0	232.69673	44
TATCCAC	45	0.0	232.69673	44
ACGCGGG	75	0.0	232.69672	44
CGCAGGC	90	0.0	219.76913	44
ATAACGG	55	0.0	211.54247	44
GTCGCCC	45	0.0	206.84154	44
ATAACCG	65	0.0	196.89723	44
AATTCGT	25	6.138471E-7	186.15738	44
ACAAATT	200	0.0	174.52255	44
ACCGTCA	20	5.876724E-5	174.52255	44
CTCGCCA	140	0.0	174.52254	44
TCGAGCT	120	0.0	164.82684	44
GATCCTT	15	0.005793926	155.13113	44
TCACGTC	15	0.005793926	155.13113	44
TCCATTG	175	0.0	146.26651	44
TCGAGTT	55	1.6425474E-9	126.925476	44
GTAAAGG	75	0.0	124.10491	44
>>END_MODULE