FastQCFastQC Report
Tue 24 May 2016
ERR841110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4321280
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA280640.6494372037914692No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA244510.5658277177132701No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAATCG188770.43683815906398105No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT181390.4197598859597157No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA132260.30606672097156395No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG124640.2884330568720379No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAAT100050.2315286211492891No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC76640.17735485781990523No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68480.15847156398104267No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC66730.15442183797393366No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT65980.1526862411137441No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.14474877813981044No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTA57000.1319053613744076No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53130.12294968157582939No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT51740.11973304206161137No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT50670.11725692387440757No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC49710.11503535989336493No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA43550.10078032434834124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCC1000.0166.8243344
ACGTAAT100.0046624565166.8243344
GTGCAAG750.0155.7027144
CGGGCTG750.0155.7027144
ACCGTCT1400.0154.9083144
AGATTCG1650.0151.6584844
GACTCTA950.0149.2638944
CGAAATC650.0141.1590644
CATACGA1700.0132.4781544
TCGATGA1250.0126.7864944
TTCTCGT6450.0122.85512544
GGTGCAC506.366463E-11116.7770344
ATCACGC650.0104.90864643
TGTTACA1600.0101.6302543
CGGAGAG1500.0100.094644
AACCGTC1400.097.4151643
TGGCCCC3550.096.3351844
TGGGCCC2900.095.8647943
TCCGCGG1100.095.371543
GAACACA84700.094.9342744
Produced by FastQC (version 0.11.5)