##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841110.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4321280
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.170638329383884	39.0	37.0	39.0	34.0	39.0
2	37.803036368853675	39.0	37.0	39.0	35.0	39.0
3	37.92208072608116	39.0	38.0	39.0	35.0	39.0
4	38.36880276214455	39.0	38.0	40.0	35.0	41.0
5	39.123898011700234	40.0	38.0	41.0	35.0	41.0
6	39.011265412100116	40.0	38.0	41.0	35.0	41.0
7	39.07856190758294	40.0	38.0	41.0	35.0	41.0
8	38.93821784286138	40.0	38.0	41.0	35.0	41.0
9	38.89148053354562	40.0	38.0	41.0	35.0	41.0
10	38.84340519475711	40.0	38.0	41.0	35.0	41.0
11	38.76159031583234	40.0	38.0	41.0	35.0	41.0
12	38.772650464677135	40.0	38.0	41.0	35.0	41.0
13	38.670709141735784	40.0	38.0	41.0	35.0	41.0
14	38.736018494520145	40.0	38.0	41.0	35.0	41.0
15	38.54708280879739	40.0	38.0	41.0	35.0	41.0
16	38.66789608634479	40.0	38.0	41.0	35.0	41.0
17	38.78075408212381	40.0	38.0	41.0	35.0	41.0
18	38.75976169098045	40.0	38.0	41.0	35.0	41.0
19	38.75951755035545	40.0	38.0	41.0	35.0	41.0
20	38.82763972711789	40.0	38.0	41.0	35.0	41.0
21	38.818136524363155	40.0	38.0	41.0	35.0	41.0
22	38.817061379961494	40.0	38.0	41.0	35.0	41.0
23	38.81023840158471	40.0	38.0	41.0	35.0	41.0
24	38.69109407397808	40.0	38.0	41.0	35.0	41.0
25	38.70469762013795	40.0	38.0	41.0	35.0	41.0
26	38.60215652681996	40.0	37.0	41.0	35.0	41.0
27	38.56399294401303	40.0	37.0	41.0	35.0	41.0
28	38.50339954465226	40.0	37.0	41.0	35.0	41.0
29	38.40526699380157	40.0	37.0	41.0	34.0	41.0
30	38.41820366202178	40.0	37.0	41.0	34.0	41.0
31	38.33032112499321	40.0	37.0	41.0	34.0	41.0
32	38.182205435297725	40.0	37.0	41.0	34.0	41.0
33	38.056753236290085	40.0	36.0	41.0	34.0	41.0
34	37.972910980545734	39.0	36.0	41.0	34.0	41.0
35	38.00989821793736	39.0	36.0	41.0	34.0	41.0
36	37.928218722163145	39.0	36.0	41.0	34.0	41.0
37	37.86507878826074	39.0	36.0	41.0	34.0	41.0
38	37.71932105219094	39.0	35.0	41.0	34.0	41.0
39	37.64730965090668	39.0	35.0	41.0	34.0	41.0
40	37.55914781021804	39.0	35.0	41.0	34.0	41.0
41	37.439863134407574	38.0	35.0	40.0	34.0	41.0
42	37.233054377716115	38.0	35.0	40.0	33.0	41.0
43	37.03240032096007	37.0	35.0	40.0	33.0	41.0
44	36.90255297107151	37.0	35.0	40.0	33.0	41.0
45	36.6725367733688	37.0	35.0	40.0	33.0	41.0
46	36.373974410482056	37.0	35.0	39.0	32.0	41.0
47	36.111634567924916	36.0	35.0	39.0	32.0	41.0
48	35.932676440885665	36.0	35.0	39.0	32.0	41.0
49	36.15847640163759	36.0	35.0	39.0	32.0	41.0
50	35.69619132654774	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	30.0
22	76.0
23	159.0
24	335.0
25	694.0
26	1360.0
27	2646.0
28	5794.0
29	11749.0
30	22323.0
31	41066.0
32	70271.0
33	123322.0
34	215255.0
35	234343.0
36	346100.0
37	539154.0
38	709833.0
39	1050628.0
40	946129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.85702384478673	20.029875407286728	32.113100747926545
2	24.623930872334125	25.730200311018958	30.71858338270142	18.9272854339455
3	23.888199792654028	25.68530620556872	27.49763958827014	22.928854413507107
4	20.037697163803315	25.53673911433649	34.29358893661137	20.131974785248815
5	17.84760533915877	27.706651732819903	32.18837936907583	22.2573635589455
6	19.753429539395732	29.734430539099527	30.095851229265403	20.416288692239338
7	22.194048985485782	30.80962122334123	23.495839195793838	23.500490595379148
8	22.3946839825237	28.921662100118482	28.533328087973935	20.150325829383885
9	21.10383960308057	32.83964473489336	27.456980339158765	18.5995353228673
10	24.406217602191944	24.930969527547393	28.238068350118482	22.42474452014218
11	23.601386626184834	26.16206309241706	27.18983727043839	23.046713010959717
12	21.356033397511847	28.070131997926538	27.46234911877962	23.11148548578199
13	20.184829494964454	25.949440906398102	29.145160693127963	24.72056890550948
14	18.29275122186019	27.396650992298575	29.52900529472749	24.781592491113745
15	18.47200366558057	33.01012662914692	28.656694312796212	19.861175392476305
16	18.693442683649288	36.59144975562796	25.74975470231043	18.96535285841232
17	23.11636829828199	34.63575607227488	23.21383941795024	19.034036211492893
18	19.60199292802133	29.254063610781987	26.799073422689574	24.344870038507107
19	20.101543986966824	26.984273178317537	25.46349692683649	27.450685907879148
20	20.03279121001185	25.02878776658768	30.975012033471565	23.96340898992891
21	20.548795727191944	31.920241224822277	26.366747815462084	21.1642152325237
22	20.081804465343602	28.333063351599524	25.177516846860186	26.40761533619668
23	25.11188814425355	25.257724563092417	28.416881109300945	21.213506183353083
24	19.12771215936019	29.20009811907583	28.65657860633886	23.015611115225116
25	21.900093237266187	29.49531757477162	24.67476736570221	23.929821822259985
26	18.986503798154803	32.28940273508215	28.60332348462327	20.120769982139784
27	22.112299352404065	27.847117468960082	27.821257708107773	22.21932547052808
28	20.687124694774347	27.02831951687392	28.687544435313384	23.597011353038344
29	23.10905760593393	27.824649487295243	26.811422633102687	22.254870273668143
30	22.5687017445915	28.476313295155208	29.26895311604528	19.686031844208014
31	21.016620314790572	31.514409300136943	26.585240195220827	20.883730189851654
32	19.423497544936797	28.11428406201099	29.63071869959818	22.83149969345403
33	18.71713856360713	27.30723751008956	34.244100016363475	19.731523909939835
34	19.173185020363203	32.70266302754927	28.052381773511698	20.071770178575825
35	20.056925914836633	30.140516303518446	25.742757319992098	24.059800461652824
36	18.311655338487316	30.50761263271512	26.549504325178276	24.631227703619288
37	19.47211560355203	30.83138336369255	26.360523127215952	23.335977905539472
38	23.59407193075241	28.00114759220046	26.064943100255043	22.33983737679209
39	19.443820893383005	30.30409525869406	27.594613030752512	22.65747081717042
40	19.33188427899277	31.194335029373903	25.416243683494077	24.05753700813925
41	21.384218201680934	30.598400486449997	26.26804219335357	21.749339118515504
42	18.921589529539247	31.8209409215057	27.128727012294263	22.12874253666079
43	19.982911607232264	28.90721418882804	27.447678176405795	23.6621960275339
44	22.337676453191417	28.627442332173715	23.799209926561026	25.235671288073842
45	18.731085176628632	30.205595023034988	29.955798830510723	21.10752096982566
46	20.593980485023863	29.972932985433058	27.076000920809506	22.357085608733566
47	19.94265419093325	32.1493100999801	25.32107590355957	22.58695980552708
48	22.588943929299905	31.59023323207849	23.880761447052766	21.94006139156884
49	19.176143466957473	30.746586092525703	22.95344794864302	27.1238224918738
50	18.96156033524536	34.839296863236655	26.70789940337528	19.491243398142704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37992.0
1	41006.5
2	55341.5
3	64884.0
4	59139.5
5	37623.0
6	14631.5
7	10970.0
8	13956.0
9	14960.5
10	13580.5
11	10816.5
12	8515.5
13	7412.0
14	7339.0
15	8088.5
16	8985.5
17	10258.5
18	12038.0
19	16235.0
20	21852.5
21	27072.5
22	31500.0
23	35216.5
24	38517.0
25	45329.5
26	59615.0
27	80994.5
28	93764.0
29	96380.0
30	113568.5
31	140445.0
32	164608.0
33	182416.0
34	223491.5
35	290920.0
36	316932.5
37	299478.0
38	282232.0
39	283306.5
40	298961.0
41	321899.0
42	345778.5
43	344757.0
44	325987.0
45	327671.5
46	346773.0
47	343342.0
48	346296.0
49	366743.0
50	409737.0
51	434886.5
52	372820.0
53	286527.5
54	228206.0
55	195253.5
56	178591.0
57	158135.0
58	135976.5
59	115052.0
60	82858.5
61	57288.0
62	45658.5
63	33010.0
64	19935.0
65	13946.5
66	9582.0
67	5785.5
68	4033.5
69	2773.5
70	1943.0
71	1598.0
72	1263.5
73	831.5
74	626.5
75	428.5
76	208.0
77	189.0
78	122.0
79	57.0
80	50.5
81	30.0
82	11.5
83	7.5
84	5.0
85	4.5
86	4.0
87	4.0
88	4.0
89	4.0
90	3.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	17207.0
25	18571.0
26	20188.0
27	24599.0
28	29812.0
29	26657.0
30	40221.0
31	63071.0
32	84248.0
33	18452.0
34	13803.0
35	13978.0
36	15369.0
37	17336.0
38	10933.0
39	11881.0
40	13774.0
41	16288.0
42	20177.0
43	35702.0
44	116803.0
45	264791.0
46	507938.0
47	414251.0
48	845475.0
49	617056.0
50	1042699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.981291626497478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19239556495098	9.760944149235845
2	10.814582371698652	2.80774495171598
3	4.179486941709431	1.6276541651840462
4	2.068879522781494	1.0742691370126203
5	1.2133811300758606	0.7875627151801909
6	0.8203386498322749	0.6389433147575976
7	0.5646031201123212	0.5130494428785899
8	0.44428581970668396	0.4613923032903956
9	0.3425425208208262	0.40019799215256563
>10	2.6180677324984263	6.937658605504861
>50	0.4270202884373263	3.9327482715709294
>100	1.064944772417796	37.071156612966476
>500	0.20603988582177857	16.98032668159956
>1k	0.03787242420670129	8.86427799946015
>5k	0.003127080897801024	2.4938872393331835
>10k+	0.0024321740316230185	5.648186418157008
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	28064	0.6494372037914692	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	24451	0.5658277177132701	No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAATCG	18877	0.43683815906398105	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	18139	0.4197598859597157	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	13226	0.30606672097156395	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	12464	0.2884330568720379	No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAAT	10005	0.2315286211492891	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7664	0.17735485781990523	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6848	0.15847156398104267	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	6673	0.15442183797393366	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6598	0.1526862411137441	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6255	0.14474877813981044	No Hit
TGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTA	5700	0.1319053613744076	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.12294968157582939	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	5174	0.11973304206161137	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	5067	0.11725692387440757	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	4971	0.11503535989336493	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4355	0.10078032434834124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04961492890995261	0.0	0.0	0.0	0.0
2	0.08284582345971564	0.0	0.0	0.0	0.0
3	0.11033767772511849	0.0	0.0	0.0	0.0
4	0.1647891365521327	0.0	0.0	0.0	0.0
5	0.19628443424170616	0.0	0.0	0.0	0.0
6	0.28556353672985785	0.0	0.0	0.0	0.0
7	0.32046060426540285	0.0	0.0	0.0	0.0
8	0.3675068498222749	0.0	0.0	0.0	0.0
9	0.4285072941350711	0.0	0.0	0.0	0.0
10	0.4659267624407583	0.0	0.0	0.0	0.0
11	0.5074190980450237	0.0	0.0	0.0	0.0
12	0.5515495408767772	0.0	0.0	0.0	0.0
13	0.5898252369668247	0.0	0.0	0.0	0.0
14	0.630715898992891	0.0	0.0	0.0	0.0
15	0.6775075903436019	0.0	0.0	0.0	0.0
16	0.7289275399881516	0.0	0.0	0.0	0.0
17	0.7823376406990521	0.0	0.0	0.0	0.0
18	0.8162164914099526	0.0	0.0	0.0	0.0
19	0.8538210900473934	0.0	0.0	0.0	0.0
20	0.8991780213270142	0.0	0.0	0.0	0.0
21	0.9368057612559242	0.0	0.0	0.0	0.0
22	0.9668431575829384	0.0	0.0	0.0	0.0
23	0.9985235856042654	0.0	0.0	0.0	0.0
24	1.0304122852488151	0.0	0.0	0.0	0.0
25	1.0629952236374407	0.0	0.0	0.0	0.0
26	1.098031138921801	0.0	0.0	0.0	0.0
27	1.1265874925947867	0.0	0.0	0.0	0.0
28	1.1595638329383886	0.0	0.0	0.0	0.0
29	1.1978858116113744	0.0	0.0	0.0	0.0
30	1.22759922985782	0.0	0.0	0.0	0.0
31	1.2603210159952607	0.0	0.0	0.0	0.0
32	1.2912377813981042	0.0	0.0	0.0	0.0
33	1.3222008293838863	0.0	0.0	0.0	0.0
34	1.3572830272511849	0.0	0.0	0.0	0.0
35	1.3874129887440758	0.0	0.0	0.0	0.0
36	1.4142106042654028	0.0	0.0	0.0	0.0
37	1.4322839529028435	0.0	0.0	0.0	0.0
38	1.4523243113151658	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCC	100	0.0	166.82433	44
ACGTAAT	10	0.0046624565	166.82433	44
GTGCAAG	75	0.0	155.70271	44
CGGGCTG	75	0.0	155.70271	44
ACCGTCT	140	0.0	154.90831	44
AGATTCG	165	0.0	151.65848	44
GACTCTA	95	0.0	149.26389	44
CGAAATC	65	0.0	141.15906	44
CATACGA	170	0.0	132.47815	44
TCGATGA	125	0.0	126.78649	44
TTCTCGT	645	0.0	122.855125	44
GGTGCAC	50	6.366463E-11	116.77703	44
ATCACGC	65	0.0	104.908646	43
TGTTACA	160	0.0	101.63025	43
CGGAGAG	150	0.0	100.0946	44
AACCGTC	140	0.0	97.41516	43
TGGCCCC	355	0.0	96.33518	44
TGGGCCC	290	0.0	95.86479	43
TCCGCGG	110	0.0	95.3715	43
GAACACA	8470	0.0	94.93427	44
>>END_MODULE