FastQCFastQC Report
Tue 24 May 2016
ERR841109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300188
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA461381.0729298346955993No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC454171.05616312589124No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA257210.5981366396073846No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT243730.5667891729384855No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC166010.386052888850441No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG134470.3127072583803313No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG99470.23131546806790773No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT90270.20992105461435637No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT79900.1858058298846469No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC76250.17731782889492273No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA72540.16869029912180586No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG68370.15899304867601138No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG67340.1565978045611029No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGA56600.13162215233380492No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG51270.11922734540908443No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA50840.11822738912810324No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT50340.11706464926649718No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC48000.11162302671418087No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT47530.11053005124427118No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC45830.1065767357148106No Hit
CCTCTTTTTCCGGCTGGAACCATGGAGGG44640.10380941484418821No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT43710.10164671870160095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTC650.0183.2192744
ACGCTTA1200.0181.946944
CGACCCG750.0172.0225444
TTGCTCG1100.0171.4210544
GTCAGCG301.6903869E-8165.4062744
AACGTTG1850.0160.9358544
TACTTTC2100.0160.6803944
GTTGGAC750.0158.7900444
CAACTAG1650.0150.3693444
TGCTATC1150.0146.7081844
CTATCAT117300.0142.9008844
GGTCAAG1050.0141.7768144
TTTCGGG354.244066E-8141.776844
GGTTCAG1600.0130.2574544
GTTAGTG1750.0119.09251444
GAACCAC1200.0115.7843944
GAACACA24500.0107.75037444
ATGGGGC550.0107.1511443
GGCACTA1100.0107.1511443
CGATCTA450.0107.1511443
Produced by FastQC (version 0.11.5)