##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841109.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4300188 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.43322640777566 39.0 38.0 39.0 35.0 39.0 2 37.88949808706038 39.0 38.0 39.0 35.0 39.0 3 38.06607315773171 39.0 38.0 39.0 35.0 39.0 4 38.607813425831615 39.0 38.0 40.0 35.0 41.0 5 39.303872528363875 40.0 39.0 41.0 35.0 41.0 6 39.330818559560655 41.0 39.0 41.0 35.0 41.0 7 39.29892320986897 40.0 39.0 41.0 35.0 41.0 8 39.24165478346528 40.0 39.0 41.0 35.0 41.0 9 39.188862440432835 40.0 38.0 41.0 35.0 41.0 10 39.11354457060947 40.0 38.0 41.0 35.0 41.0 11 38.9950581230402 40.0 38.0 41.0 35.0 41.0 12 38.96616822334279 40.0 38.0 41.0 35.0 41.0 13 38.787272091359725 40.0 38.0 41.0 35.0 41.0 14 38.82732987487989 40.0 38.0 41.0 35.0 41.0 15 38.773549900609 40.0 38.0 41.0 35.0 41.0 16 38.86009309360428 40.0 38.0 41.0 35.0 41.0 17 38.90564598571039 40.0 38.0 41.0 35.0 41.0 18 38.904411155977364 40.0 38.0 41.0 35.0 41.0 19 38.84643810921755 40.0 38.0 41.0 35.0 41.0 20 38.7771869508961 40.0 38.0 41.0 35.0 41.0 21 38.84266548346258 40.0 38.0 41.0 35.0 41.0 22 38.818934195435176 40.0 38.0 41.0 35.0 41.0 23 38.7682459464563 40.0 38.0 41.0 35.0 41.0 24 38.57591272753656 40.0 37.0 41.0 35.0 41.0 25 38.54272132701953 40.0 37.0 41.0 35.0 41.0 26 38.474089085502364 40.0 37.0 41.0 35.0 41.0 27 38.42886269418309 40.0 37.0 41.0 34.0 41.0 28 38.34644669901013 40.0 37.0 41.0 34.0 41.0 29 38.1896297605784 40.0 36.0 41.0 34.0 41.0 30 38.181171586310256 40.0 36.0 41.0 34.0 41.0 31 38.041067280458726 40.0 36.0 41.0 34.0 41.0 32 37.89154004976571 39.0 35.0 41.0 34.0 41.0 33 37.782439220866294 39.0 35.0 41.0 34.0 41.0 34 37.699664871529905 39.0 35.0 41.0 34.0 41.0 35 37.7201883585716 39.0 35.0 41.0 34.0 41.0 36 37.66425372043639 39.0 35.0 41.0 34.0 41.0 37 37.586022809381284 39.0 35.0 41.0 34.0 41.0 38 37.517793710053276 39.0 35.0 41.0 34.0 41.0 39 37.33961697360738 38.0 35.0 40.0 34.0 41.0 40 37.23693365928384 38.0 35.0 40.0 34.0 41.0 41 37.196995239447496 38.0 35.0 40.0 34.0 41.0 42 37.02985311518535 37.0 35.0 40.0 34.0 41.0 43 36.87500922103078 37.0 35.0 40.0 34.0 41.0 44 36.65800816069282 37.0 35.0 39.0 33.0 41.0 45 36.46634530866253 37.0 35.0 39.0 33.0 41.0 46 36.19027631924193 36.0 35.0 39.0 32.0 41.0 47 35.93557864714949 36.0 35.0 39.0 32.0 41.0 48 35.86669369105639 36.0 35.0 39.0 32.0 41.0 49 35.92512133376574 36.0 35.0 39.0 32.0 41.0 50 35.81814827128039 36.0 34.0 39.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 6.0 21 24.0 22 55.0 23 149.0 24 255.0 25 670.0 26 1411.0 27 3038.0 28 6452.0 29 12888.0 30 24489.0 31 41929.0 32 70197.0 33 120380.0 34 239023.0 35 231546.0 36 346961.0 37 542450.0 38 683922.0 39 970361.0 40 1003980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.1477526098859 19.909641159874873 32.942606230239235 2 24.46330253467988 19.43949892423308 33.706782122083965 22.390416419003074 3 23.475206200287058 21.918832385932895 28.37308508372192 26.23287633005813 4 20.713675774175456 24.670502778018076 29.19581655499713 25.420004892809338 5 15.392629345507686 26.148717218875085 31.533156224797615 26.92549721081962 6 20.219674116573508 26.95621679796325 26.511003704954295 26.313105380508944 7 20.2249529555452 26.468470680816747 23.150220408968167 30.156355954669888 8 21.87090424883749 26.4457739987182 28.354364971950062 23.32895678049425 9 21.24637341437165 27.725438980807347 29.573241914074455 21.454945690746545 10 27.785692160435776 22.315838284279664 25.76650137156794 24.13196818371662 11 23.336933175944864 23.44288203213441 25.230827117326033 27.989357674594693 12 22.120916573880027 26.5083294032726 26.719738764909813 24.651015257937562 13 26.388311394757626 21.804721095914875 24.97676845756511 26.83019905176239 14 19.791018439193824 23.883676713669264 24.465418721228 31.859886125908915 15 21.94817994003983 33.437538079730466 24.677642000768337 19.93663997946136 16 20.484150925494422 32.298773914070736 24.310402242878684 22.90667291755616 17 21.815092735480402 39.62431409975564 18.540468463239282 20.02012470152468 18 20.713466481000363 28.760393731622898 21.793954124796404 28.732185662580335 19 24.23124291310054 24.863982690989324 21.942970865459834 28.961803530450297 20 21.027452753228463 23.210961939338468 29.405714354814254 26.355870952618815 21 21.219351339987927 32.82528577820318 23.769751462029102 22.185611419779786 22 25.266802288644126 25.905425530232634 22.388835092791293 26.438937088331954 23 29.050706620268695 22.928439407765428 28.399572297769303 19.62128167419657 24 21.712632098875677 26.049744801855173 26.403217719783413 25.834405379485737 25 21.992549794029756 25.614714320111542 23.001099379009514 29.39163650684919 26 21.71651617185383 31.729917239164816 27.061496528969105 19.492070060012253 27 22.982366603386325 29.178234725054626 22.842669160615188 24.996729510943865 28 23.27532923049474 23.6875561835341 24.19114001155 28.845974574421163 29 21.52268651744368 29.615328229024325 25.492414225059584 23.369571028472407 30 24.900832907990143 28.893801841688223 26.689592186684557 19.515773063637077 31 25.28694328468787 29.441929316159506 24.473744375930913 20.797383023221713 32 21.292352577237107 29.700323435409736 24.602025232330387 24.405298755022773 33 20.592806072159426 23.470777482575564 31.908507327519352 24.027909117745654 34 22.953374576694696 29.755365006259332 24.70658993911435 22.58467047793162 35 19.75862873428099 25.9676794387235 23.654263110450923 30.61942871654459 36 19.49962804486118 26.50171514052116 28.288338814466957 25.710318000150707 37 18.809472712426807 33.97739379770738 23.24746058010141 23.9656729097644 38 25.905259224017968 25.10195621756272 23.687642934492157 25.305141623927152 39 19.671823257008835 29.03873916427721 26.800716863119984 24.488720715593974 40 15.840573445643201 26.60387054247966 30.98605504059665 26.56950097128049 41 17.999934031533957 29.98813082989876 24.10348596958957 27.90844916897771 42 18.845272525315817 30.234813746533725 24.225378733937305 26.69453499421316 43 19.72883522545844 23.946397854829826 24.577676790294078 31.747090129417654 44 19.460454155255523 26.802785364714882 22.614541378654355 31.122219101375237 45 19.048896230673478 23.841898642362224 35.24012857601003 21.869076550954272 46 18.532473532679735 29.84045792812269 27.28986474815328 24.337203791044296 47 19.55652955013334 30.345115638554805 30.211179733456365 19.88717507785549 48 24.20185953672716 25.533323940881548 23.654889825696905 26.60992669669439 49 17.37414870287871 30.1725656528183 24.865376631088708 27.587909013214286 50 17.086693605970975 28.124953587394508 32.142657211633484 22.645695595001033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29987.0 1 27403.5 2 27324.0 3 28059.0 4 24006.0 5 15448.5 6 6389.5 7 4181.0 8 5357.0 9 6129.0 10 6497.5 11 6766.0 12 6930.5 13 7325.5 14 7733.5 15 7353.0 16 7258.5 17 8088.5 18 8681.5 19 9663.0 20 11567.0 21 13703.5 22 15040.0 23 19722.0 24 26452.5 25 29650.0 26 32319.5 27 39162.0 28 44675.0 29 51565.0 30 69088.0 31 98637.5 32 146020.0 33 169034.5 34 162021.5 35 167384.0 36 166364.0 37 158199.0 38 165618.5 39 179935.0 40 195904.5 41 217874.5 42 239907.0 43 264902.5 44 287952.5 45 316084.0 46 353829.5 47 364368.5 48 387096.5 49 463759.5 50 553958.0 51 589745.5 52 514654.5 53 385601.5 54 309889.0 55 280511.5 56 265350.5 57 259568.0 58 243702.0 59 210796.0 60 167286.0 61 126811.0 62 96361.0 63 74292.5 64 55875.5 65 42790.0 66 28403.0 67 17108.0 68 12396.0 69 10120.0 70 8657.5 71 6866.5 72 5928.0 73 4140.5 74 2234.0 75 1491.5 76 941.5 77 727.5 78 480.0 79 216.0 80 57.5 81 33.5 82 31.0 83 25.0 84 18.5 85 10.0 86 3.5 87 2.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 14132.0 25 13262.0 26 15021.0 27 20544.0 28 28628.0 29 23745.0 30 38392.0 31 71771.0 32 77433.0 33 15205.0 34 12817.0 35 14460.0 36 17363.0 37 17350.0 38 11927.0 39 12250.0 40 15246.0 41 18432.0 42 25871.0 43 41073.0 44 137663.0 45 311401.0 46 485132.0 47 490372.0 48 764179.0 49 731218.0 50 875301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.94278405641849 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66399076993858 8.916959185557998 2 10.770546332750488 2.572606180433784 3 4.1097771662846085 1.472465436508098 4 2.1118000953187064 1.0088309003486116 5 1.3353610687618418 0.7973964440785438 6 0.8350298592369826 0.5983548773717288 7 0.587317714835887 0.49099460485559215 8 0.4523792728966622 0.4322134374243557 9 0.34866084088334426 0.3747583018439161 >10 2.8649708072695934 7.036556238999464 >50 0.5074751399623638 4.346190807299933 >100 1.1831099294137992 37.64451832568347 >500 0.177148147075887 13.440633793268269 >1k 0.0460660086476408 9.77422161916947 >5k 0.004119724350602023 3.4136086020061533 >10k+ 0.002247122373055649 7.679691245150624 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 46138 1.0729298346955993 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 45417 1.05616312589124 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 25721 0.5981366396073846 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 24373 0.5667891729384855 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 16601 0.386052888850441 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 13447 0.3127072583803313 No Hit CTCTTTTTCCGGCTGGAACCATGGAGGG 9947 0.23131546806790773 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 9027 0.20992105461435637 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT 7990 0.1858058298846469 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 7625 0.17731782889492273 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 7254 0.16869029912180586 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 6837 0.15899304867601138 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG 6734 0.1565978045611029 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGA 5660 0.13162215233380492 No Hit TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG 5127 0.11922734540908443 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTA 5084 0.11822738912810324 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 5034 0.11706464926649718 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC 4800 0.11162302671418087 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT 4753 0.11053005124427118 No Hit CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC 4583 0.1065767357148106 No Hit CCTCTTTTTCCGGCTGGAACCATGGAGGG 4464 0.10380941484418821 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT 4371 0.10164671870160095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.018092232246590147 0.0 0.0 0.0 0.0 2 0.03646352205996575 0.0 0.0 0.0 0.0 3 0.0628577169184231 0.0 0.0 0.0 0.0 4 0.10173973789052944 0.0 0.0 0.0 0.0 5 0.12904086984103952 0.0 0.0 0.0 0.0 6 0.1742016860658185 0.0 0.0 0.0 0.0 7 0.2099443094115885 0.0 0.0 0.0 0.0 8 0.2564306490785984 0.0 0.0 0.0 0.0 9 0.300940330980878 0.0 0.0 0.0 0.0 10 0.33968282316959164 0.0 0.0 0.0 0.0 11 0.3715186405803653 0.0 0.0 0.0 0.0 12 0.40242426610185417 0.0 0.0 0.0 0.0 13 0.4311671954807557 0.0 0.0 0.0 0.0 14 0.4629099937026009 0.0 0.0 0.0 0.0 15 0.4970945456338188 0.0 0.0 0.0 0.0 16 0.5276513491968258 0.0 0.0 0.0 0.0 17 0.5803234649275799 0.0 0.0 0.0 2.3254797232121015E-5 18 0.6138103729418342 0.0 0.0 0.0 2.3254797232121015E-5 19 0.6534830570198327 0.0 0.0 0.0 2.3254797232121015E-5 20 0.688923368001585 0.0 0.0 0.0 2.3254797232121015E-5 21 0.7296657727522611 0.0 0.0 0.0 2.3254797232121015E-5 22 0.7522926904591148 0.0 0.0 0.0 2.3254797232121015E-5 23 0.78173326375498 0.0 0.0 0.0 2.3254797232121015E-5 24 0.8134295523823609 0.0 0.0 0.0 2.3254797232121015E-5 25 0.8386610073792122 0.0 0.0 0.0 2.3254797232121015E-5 26 0.8756593897755168 0.0 0.0 0.0 2.3254797232121015E-5 27 0.9006117872055827 0.0 0.0 0.0 2.3254797232121015E-5 28 0.9327266621831417 0.0 0.0 0.0 2.3254797232121015E-5 29 0.9632834657461488 0.0 0.0 0.0 2.3254797232121015E-5 30 0.9904450689132661 0.0 0.0 0.0 2.3254797232121015E-5 31 1.0149091156014574 0.0 0.0 0.0 2.3254797232121015E-5 32 1.0413265652571468 0.0 0.0 0.0 2.3254797232121015E-5 33 1.068650952004889 0.0 0.0 0.0 2.3254797232121015E-5 34 1.0973008621948621 0.0 0.0 0.0 2.3254797232121015E-5 35 1.1219044376664462 0.0 0.0 0.0 2.3254797232121015E-5 36 1.1404617658576788 0.0 0.0 0.0 2.3254797232121015E-5 37 1.156647104731235 0.0 0.0 0.0 2.3254797232121015E-5 38 1.169134930844884 0.0 0.0 0.0 2.3254797232121015E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGTTC 65 0.0 183.21927 44 ACGCTTA 120 0.0 181.9469 44 CGACCCG 75 0.0 172.02254 44 TTGCTCG 110 0.0 171.42105 44 GTCAGCG 30 1.6903869E-8 165.40627 44 AACGTTG 185 0.0 160.93585 44 TACTTTC 210 0.0 160.68039 44 GTTGGAC 75 0.0 158.79004 44 CAACTAG 165 0.0 150.36934 44 TGCTATC 115 0.0 146.70818 44 CTATCAT 11730 0.0 142.90088 44 GGTCAAG 105 0.0 141.77681 44 TTTCGGG 35 4.244066E-8 141.7768 44 GGTTCAG 160 0.0 130.25745 44 GTTAGTG 175 0.0 119.092514 44 GAACCAC 120 0.0 115.78439 44 GAACACA 2450 0.0 107.750374 44 ATGGGGC 55 0.0 107.15114 43 GGCACTA 110 0.0 107.15114 43 CGATCTA 45 0.0 107.15114 43 >>END_MODULE