FastQCFastQC Report
Tue 24 May 2016
ERR841108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635555
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT139110.3826375890338614No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA126260.34729222910944824No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG114650.3153576276524492No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA113970.31348721171870597No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA109290.30061434911588464No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT107760.2964059132649623No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA101510.27921459034452784No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA81380.22384477748239265No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC75990.20901898059580998No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT71270.1960360935262979No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG69660.191607608741994No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA67670.18613389152412768No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC65600.1804401253728798No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA62400.17163816803761736No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC61230.16841995238691204No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.16514672450286133No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59620.16399146760260813No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.16396396148593545No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA52890.14547985108188433No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG49310.13563266131305948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA48370.13304708634582615No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG48260.1327445190624265No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT47840.13158926216217331No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47060.1294437850617031No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA46910.12903119331161267No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG45950.12639060611103395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA44700.12295234152694705No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC42590.1171485509090084No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA42050.11566322060868285No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA41450.11401285360832114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39840.10958436882401723No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG39210.10785148347363745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAACG1250.0167.8311644
CACGAGT750.0163.1691944
ACGGTAT1200.0160.2554544
ACGTATC1750.0159.839244
ACGCTTA900.0155.3992244
ATCGTAA800.0152.971144
GTATACT407.2759576E-12152.971144
CCGCCTC1100.0150.9844844
GCTTATG650.0147.9281244
TGTGCCG500.0139.859344
CTGCGAT1850.0132.2993544
TCGATTG700.0124.8743744
CCCTAGT1050.0116.54941644
CGAATAA306.314127E-6116.54941644
GTCCGGG850.0113.121544
GGTTAGA700.0112.3869344
TGTACAC1800.0111.69318444
CCGACCC402.0072548E-7109.2650744
TAGGCAA1700.0107.9796144
TGGCGAG1000.0106.6383343
Produced by FastQC (version 0.11.5)