##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841108.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3635555
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.981566775911794	39.0	35.0	39.0	31.0	39.0
2	36.98982466225927	39.0	37.0	39.0	33.0	39.0
3	37.03674129534555	39.0	35.0	39.0	33.0	39.0
4	37.39173386181752	39.0	35.0	39.0	33.0	40.0
5	38.12842220788848	40.0	36.0	41.0	34.0	41.0
6	38.17606912837242	40.0	36.0	41.0	34.0	41.0
7	38.16558214632979	40.0	36.0	41.0	34.0	41.0
8	37.957999810207795	39.0	36.0	41.0	34.0	41.0
9	37.94557199657274	39.0	36.0	41.0	34.0	41.0
10	37.875544174135726	39.0	36.0	40.0	34.0	41.0
11	37.862650957006565	39.0	36.0	40.0	34.0	41.0
12	37.74141912307749	39.0	36.0	40.0	33.0	41.0
13	37.62386100609123	39.0	35.0	40.0	33.0	41.0
14	37.61821317515483	39.0	35.0	40.0	33.0	41.0
15	37.49269506306465	38.0	35.0	40.0	33.0	41.0
16	37.55382273132988	39.0	35.0	40.0	33.0	41.0
17	37.82059107894118	39.0	35.0	40.0	34.0	41.0
18	37.89176508125995	39.0	35.0	41.0	34.0	41.0
19	37.92522159615245	40.0	35.0	41.0	34.0	41.0
20	37.99471139894734	40.0	35.0	41.0	34.0	41.0
21	37.99547276825684	40.0	35.0	41.0	34.0	41.0
22	38.05677895121928	40.0	35.0	41.0	34.0	41.0
23	37.99921937640883	40.0	35.0	41.0	34.0	41.0
24	37.931363987066625	40.0	35.0	41.0	34.0	41.0
25	37.888060805901006	40.0	35.0	41.0	34.0	41.0
26	37.83243512848563	40.0	35.0	41.0	34.0	41.0
27	37.81013081915346	39.0	35.0	41.0	33.0	41.0
28	37.834480453769565	39.0	35.0	41.0	34.0	41.0
29	37.75159545225708	39.0	35.0	41.0	33.0	41.0
30	37.78533401328793	39.0	35.0	41.0	34.0	41.0
31	37.693478066458816	39.0	35.0	41.0	33.0	41.0
32	37.64584252939828	39.0	35.0	41.0	33.0	41.0
33	37.477410233121404	39.0	35.0	41.0	33.0	41.0
34	37.483013533971864	39.0	35.0	41.0	33.0	41.0
35	37.547972696696306	39.0	35.0	41.0	33.0	41.0
36	37.60367358591347	39.0	35.0	41.0	34.0	41.0
37	37.49883202162118	39.0	35.0	41.0	33.0	41.0
38	37.420434939763524	39.0	35.0	41.0	33.0	41.0
39	37.45948092411709	39.0	35.0	41.0	34.0	41.0
40	37.35430280440218	39.0	35.0	40.0	33.0	41.0
41	37.19755064261149	38.0	35.0	40.0	33.0	41.0
42	37.12973103340727	38.0	35.0	40.0	33.0	41.0
43	36.979011225157166	38.0	35.0	40.0	33.0	41.0
44	36.86663741259516	37.0	35.0	40.0	33.0	41.0
45	36.626983524384514	37.0	35.0	40.0	32.0	41.0
46	36.429295201834464	37.0	35.0	40.0	32.0	41.0
47	36.11240988860567	37.0	35.0	39.0	31.0	41.0
48	36.06488634892582	37.0	34.0	39.0	31.0	41.0
49	36.192565399555676	37.0	34.0	39.0	32.0	41.0
50	35.93524397452532	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	20.0
22	56.0
23	175.0
24	507.0
25	1020.0
26	2063.0
27	4442.0
28	8834.0
29	17442.0
30	33582.0
31	62185.0
32	108580.0
33	197206.0
34	356331.0
35	196474.0
36	291663.0
37	412211.0
38	567937.0
39	850060.0
40	524761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.458727759585535	18.247172715032505	27.294099525381952
2	23.65129945771691	24.244441357646906	30.82827793830653	21.275981246329653
3	24.48217672404901	23.06792222920572	27.48713745219093	24.96276359455434
4	21.542488010771397	24.65048665196923	32.74286869542615	21.06415664183323
5	17.90428146459069	27.73903846867947	31.143443023142268	23.21323704358757
6	21.238737964354822	31.137941799807734	28.936379727441892	18.686940508395555
7	25.049957984406785	27.071850102666577	27.939007936889965	19.93918397603667
8	21.67644279896742	28.241025097956157	27.709771960539726	22.372760142536695
9	21.836940989752595	28.510254967948494	28.532369885753344	21.12043415654556
10	23.815483468136225	25.157864480113766	30.42932922208576	20.597322829664247
11	24.24732949989754	26.31221367851676	26.870560340855796	22.569896480729902
12	21.7405320508148	27.694781126953107	28.52312783055132	22.041558991680777
13	22.513618966017567	25.595349265792983	30.203393979736244	21.687637788453205
14	20.246812384904093	28.42121766827898	28.816315528165575	22.515654418651348
15	22.785076831460398	28.067131428351377	28.092684610740314	21.05510712944791
16	21.535721506069912	33.13210775246145	24.687647415594043	20.64452332587459
17	23.84153176062527	32.51126719304205	23.881910739900786	19.765290306431893
18	20.921042316785197	29.862840749211607	25.53717382903023	23.67894310497297
19	23.291711994454765	26.537296231249424	28.247296492557535	21.923695281738276
20	23.076806704891002	25.56437737841953	28.55110155120745	22.807714365482024
21	20.5265495914654	28.722437151961667	27.104829936557138	23.646183320015787
22	22.766812769989727	30.70364772366255	26.93418198871974	19.59535751762798
23	24.913335102893505	27.513185744679973	27.959087402061034	19.61439175036549
24	21.048588179796482	29.353977590766746	26.088451419384384	23.508982810052387
25	22.26614831110424	27.17600421690453	29.320040772278844	21.23780669971239
26	23.138980749160684	28.498507183263687	28.80754888852564	19.554963179049988
27	23.23645388448491	28.821216821315748	27.375598991757517	20.566730302441833
28	22.233554135859205	28.31950865023703	28.628554837658076	20.818382376245694
29	22.964334998803572	29.11472018584807	26.482977983144796	21.437966832203564
30	22.777787381656207	30.118963242115697	26.664448170749278	20.43880120547882
31	21.84364616083286	28.399982172470857	28.7976059842238	20.95876568247248
32	22.92286444412713	30.04721025211389	28.521023907892605	18.508901395866378
33	22.632697547683925	26.145716015743265	30.423251589464122	20.79833484710869
34	21.41450166226692	30.19233607461562	27.11380252116823	21.27935974194923
35	20.541592126341342	28.497395392445263	28.508580261212522	22.45243222000087
36	21.31987549039471	28.43782808096808	28.545853056563463	21.696443372073748
37	20.446875194843116	30.2257309587732	24.693976700930246	24.633417145453436
38	22.153812616681385	28.651358814125853	26.69930950704094	22.49551906215182
39	22.667788319234784	30.60559891573585	26.34506394977489	20.381548815254476
40	19.353967335384915	29.061246387767792	27.137974061022845	24.446812215824444
41	20.48800519292169	28.070368361885816	27.975074729938537	23.466551715253956
42	21.6360235043504	28.74658196311961	26.53848752144264	23.078907011087352
43	20.260388561665906	26.882951629684854	29.22758003509729	23.62907977355195
44	20.6850271301531	29.2271945934364	26.034153378925566	24.053624897484934
45	21.636600130038364	27.43358577309996	29.175874401904757	21.75393969495692
46	21.688227404490977	30.423426931452717	25.330009085795872	22.55833657826043
47	22.152830275486874	30.44993755146886	27.27322994608617	20.124002226958098
48	20.15555330082185	30.454149462520913	26.477748860540334	22.912548376116906
49	17.912683015752805	34.34204734585475	24.91807919233716	22.82719044605528
50	22.28245235635902	28.70756192975408	27.272396243614033	21.73758947027287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50270.0
1	49877.0
2	53614.0
3	56603.5
4	52469.5
5	34320.0
6	14245.0
7	11169.0
8	14264.0
9	15507.0
10	15119.5
11	14037.0
12	12715.0
13	11362.5
14	9742.5
15	8271.5
16	7838.0
17	7866.5
18	8356.0
19	10767.0
20	13364.0
21	18733.5
22	25431.5
23	28252.0
24	32147.5
25	38370.0
26	40796.5
27	43514.0
28	55479.0
29	75515.0
30	96143.5
31	116853.5
32	138943.5
33	149355.5
34	159691.0
35	182787.0
36	201337.5
37	214713.0
38	226109.0
39	240639.5
40	256666.5
41	261921.5
42	269568.0
43	283411.0
44	288017.0
45	283754.0
46	299948.0
47	314297.5
48	319083.0
49	337129.0
50	349737.0
51	339302.0
52	309269.5
53	274782.5
54	235545.5
55	203942.5
56	186817.0
57	168727.0
58	140310.0
59	106783.0
60	79575.5
61	58270.0
62	41357.5
63	30139.5
64	20682.5
65	14534.0
66	9902.0
67	6629.0
68	4757.5
69	3280.5
70	2128.5
71	1544.5
72	1168.5
73	741.5
74	386.0
75	393.5
76	468.0
77	505.5
78	570.5
79	485.0
80	356.5
81	244.0
82	110.5
83	40.5
84	10.0
85	7.0
86	2.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	27221.0
25	29529.0
26	20534.0
27	24495.0
28	35865.0
29	27091.0
30	37927.0
31	57355.0
32	72538.0
33	16825.0
34	13897.0
35	14698.0
36	17794.0
37	19478.0
38	10758.0
39	11043.0
40	15713.0
41	15040.0
42	21072.0
43	33725.0
44	96924.0
45	242473.0
46	404409.0
47	368422.0
48	651255.0
49	525597.0
50	823877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.370699291528949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20704438514588	7.047425975378727
2	10.071353521623307	1.8875125061962619
3	3.8999213419502494	1.096349704680955
4	1.9878429768331096	0.7450991509872327
5	1.2176701833343606	0.5705210562143609
6	0.8428971060792492	0.47391211888611723
7	0.5862035426530051	0.38451959852811923
8	0.45267685793957835	0.3393518969588766
9	0.37079429502118566	0.31271416538922
>10	3.0578591672353603	5.777260378605234
>50	0.5050584583734222	3.354224182891458
>100	1.325778451560072	32.60319015714882
>500	0.3627091790437322	23.588882239297124
>1k	0.10455031768444609	15.790393919973193
>5k	0.004825399277743665	2.966862081128185
>10k+	0.002814816245350471	3.061780867736114
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	13911	0.3826375890338614	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	12626	0.34729222910944824	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	11465	0.3153576276524492	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	11397	0.31348721171870597	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10929	0.30061434911588464	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	10776	0.2964059132649623	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	10151	0.27921459034452784	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	8138	0.22384477748239265	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7599	0.20901898059580998	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	7127	0.1960360935262979	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	6966	0.191607608741994	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6767	0.18613389152412768	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	6560	0.1804401253728798	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	6240	0.17163816803761736	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	6123	0.16841995238691204	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6004	0.16514672450286133	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5962	0.16399146760260813	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5961	0.16396396148593545	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	5289	0.14547985108188433	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	4931	0.13563266131305948	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4837	0.13304708634582615	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4826	0.1327445190624265	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	4784	0.13158926216217331	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4706	0.1294437850617031	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4691	0.12903119331161267	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	4595	0.12639060611103395	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	4470	0.12295234152694705	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	4259	0.1171485509090084	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4205	0.11566322060868285	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	4145	0.11401285360832114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3984	0.10958436882401723	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3921	0.10785148347363745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04409230502633023	0.0	0.0	0.0	0.0
2	0.06901284673179198	0.0	0.0	0.0	0.0
3	0.09734414690466793	0.0	0.0	0.0	0.0
4	0.1562897549342535	0.0	0.0	0.0	0.0
5	0.18555626307400108	0.0	0.0	0.0	0.0
6	0.2706601880593197	0.0	0.0	0.0	0.0
7	0.30397009534995345	0.0	0.0	0.0	0.0
8	0.34814491872630177	0.0	0.0	0.0	0.0
9	0.4067054411224696	0.0	0.0	0.0	0.0
10	0.4408130257966115	0.0	0.0	0.0	0.0
11	0.47816633223813143	0.0	0.0	0.0	0.0
12	0.5148869979961794	0.0	0.0	0.0	0.0
13	0.5535055858046433	0.0	0.0	0.0	0.0
14	0.589263537479147	0.0	0.0	0.0	0.0
15	0.6339609770722765	0.0	0.0	0.0	0.0
16	0.6762378783982088	0.0	0.0	0.0	0.0
17	0.7154340946567993	0.0	0.0	0.0	0.0
18	0.7470661288303987	0.0	0.0	0.0	0.0
19	0.7801009749543054	0.0	0.0	0.0	0.0
20	0.8153913226453733	0.0	0.0	0.0	0.0
21	0.8552751918207812	0.0	0.0	0.0	0.0
22	0.8841841204437837	0.0	0.0	0.0	0.0
23	0.9110851025496794	0.0	0.0	0.0	0.0
24	0.9415893859396983	0.0	0.0	0.0	0.0
25	0.9722587060297534	0.0	0.0	0.0	0.0
26	1.0025429404863906	0.0	0.0	0.0	0.0
27	1.0325246076596284	0.0	0.0	0.0	0.0
28	1.0654219232001716	0.0	0.0	0.0	0.0
29	1.0969164267904075	0.0	0.0	0.0	0.0
30	1.1301438157310233	0.0	0.0	0.0	0.0
31	1.1583650914372083	0.0	0.0	0.0	0.0
32	1.1887043381271911	0.0	0.0	0.0	0.0
33	1.2182734135503384	0.0	0.0	0.0	0.0
34	1.2490802642237568	0.0	0.0	0.0	0.0
35	1.2759812463296525	0.0	0.0	0.0	0.0
36	1.3015344287185864	0.0	0.0	0.0	0.0
37	1.3178180497888219	0.0	0.0	0.0	0.0
38	1.3336615729922943	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACAACG	125	0.0	167.83116	44
CACGAGT	75	0.0	163.16919	44
ACGGTAT	120	0.0	160.25545	44
ACGTATC	175	0.0	159.8392	44
ACGCTTA	90	0.0	155.39922	44
ATCGTAA	80	0.0	152.9711	44
GTATACT	40	7.2759576E-12	152.9711	44
CCGCCTC	110	0.0	150.98448	44
GCTTATG	65	0.0	147.92812	44
TGTGCCG	50	0.0	139.8593	44
CTGCGAT	185	0.0	132.29935	44
TCGATTG	70	0.0	124.87437	44
CCCTAGT	105	0.0	116.549416	44
CGAATAA	30	6.314127E-6	116.549416	44
GTCCGGG	85	0.0	113.1215	44
GGTTAGA	70	0.0	112.38693	44
TGTACAC	180	0.0	111.693184	44
CCGACCC	40	2.0072548E-7	109.26507	44
TAGGCAA	170	0.0	107.97961	44
TGGCGAG	100	0.0	106.63833	43
>>END_MODULE