##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841107.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7184162
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.75616140059203	39.0	38.0	39.0	35.0	39.0
2	38.0815743019158	39.0	38.0	39.0	35.0	39.0
3	38.164222354674074	39.0	38.0	39.0	35.0	39.0
4	38.581786156826645	39.0	38.0	40.0	35.0	41.0
5	39.45297461276625	41.0	39.0	41.0	36.0	41.0
6	39.449254206684095	41.0	39.0	41.0	36.0	41.0
7	39.44316845861772	41.0	39.0	41.0	36.0	41.0
8	39.38184815431501	40.0	39.0	41.0	36.0	41.0
9	39.31839287588448	40.0	39.0	41.0	36.0	41.0
10	39.33462330053248	40.0	39.0	41.0	36.0	41.0
11	39.27429991138841	40.0	39.0	41.0	36.0	41.0
12	39.1956661055249	40.0	39.0	41.0	35.0	41.0
13	39.11889069316644	40.0	39.0	41.0	35.0	41.0
14	39.098204494831826	40.0	38.0	41.0	35.0	41.0
15	39.11854326224826	40.0	38.0	41.0	35.0	41.0
16	39.08415330834689	40.0	38.0	41.0	35.0	41.0
17	39.154626941875755	40.0	39.0	41.0	35.0	41.0
18	39.10760141544692	40.0	39.0	41.0	35.0	41.0
19	39.03956717568451	40.0	39.0	41.0	35.0	41.0
20	39.081352424959235	40.0	39.0	41.0	35.0	41.0
21	39.12340520717657	40.0	38.0	41.0	35.0	41.0
22	39.157403466124514	40.0	39.0	41.0	35.0	41.0
23	39.103981647407174	40.0	38.0	41.0	35.0	41.0
24	39.006515025691236	40.0	38.0	41.0	35.0	41.0
25	38.982802205449566	40.0	38.0	41.0	35.0	41.0
26	38.95258742517501	40.0	38.0	41.0	35.0	41.0
27	38.89024247380472	40.0	38.0	41.0	35.0	41.0
28	38.878061852987884	40.0	38.0	41.0	35.0	41.0
29	38.80264195814713	40.0	38.0	41.0	35.0	41.0
30	38.840861087202406	40.0	38.0	41.0	35.0	41.0
31	38.79509853625891	40.0	38.0	41.0	35.0	41.0
32	38.72149516245277	40.0	38.0	41.0	35.0	41.0
33	38.71315045490699	40.0	38.0	41.0	35.0	41.0
34	38.71811337232226	40.0	38.0	41.0	35.0	41.0
35	38.68098358577407	40.0	38.0	41.0	35.0	41.0
36	38.64925833107056	40.0	37.0	41.0	35.0	41.0
37	38.64000357661786	40.0	37.0	41.0	35.0	41.0
38	38.56477923456322	40.0	37.0	41.0	35.0	41.0
39	38.54129855181123	40.0	37.0	41.0	35.0	41.0
40	38.49449809171575	40.0	37.0	41.0	35.0	41.0
41	38.43244851047554	40.0	37.0	41.0	35.0	41.0
42	38.334411138381306	40.0	36.0	41.0	34.0	41.0
43	38.24518088738556	40.0	36.0	41.0	34.0	41.0
44	38.152089013201085	40.0	36.0	41.0	34.0	41.0
45	37.968970429222814	40.0	36.0	41.0	34.0	41.0
46	37.68636423753022	39.0	35.0	41.0	33.0	41.0
47	37.49771929098712	39.0	35.0	41.0	33.0	41.0
48	37.38345317667969	39.0	35.0	41.0	33.0	41.0
49	37.57940171423437	39.0	35.0	41.0	33.0	41.0
50	37.0175583167341	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	39.0
22	79.0
23	158.0
24	377.0
25	749.0
26	1557.0
27	3310.0
28	7034.0
29	14422.0
30	28033.0
31	51178.0
32	88686.0
33	159481.0
34	279211.0
35	256100.0
36	414443.0
37	701448.0
38	916154.0
39	1575948.0
40	2685745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.237533062311236	17.73524873186323	30.027218205825534
2	25.552319115298346	21.39915553129231	32.27730666429849	20.771218689110853
3	22.633453978348484	19.707072307111115	32.76838690441558	24.891086810124825
4	20.911583007176063	21.37975173722419	34.3819501843082	23.32671507129154
5	17.68369087445411	26.10233455203265	33.15281587469771	23.061158698815536
6	20.227828938155902	27.28198501091707	30.67432221044013	21.815863840486895
7	24.6302073923166	26.279348934503428	26.40882819735969	22.681615475820283
8	25.255346413402147	24.64681336528881	28.580591584655245	21.517248636653793
9	22.14284700150136	26.524916893577842	29.534551141803316	21.79768496311748
10	23.91610879598762	22.831194508141657	29.177306970527667	24.07538972534305
11	24.52355612248165	24.07547324239069	27.988608831482363	23.412361803645297
12	23.547102640502818	24.660969504863615	29.78067588119533	22.011251973438238
13	23.850311281956056	23.31748643752744	29.123772542991095	23.708429737525407
14	20.09583024436253	25.856599558862953	29.503928224335695	24.543641972438817
15	21.280032382343272	28.40538673821665	29.203921069708617	21.110659809731462
16	22.245879199271954	30.77940057587788	25.703902556763058	21.270817668087105
17	22.833393790396155	30.71435471527507	25.19325148848258	21.259000005846193
18	21.932940821768774	25.57131924363621	27.539997010089696	24.955742924505323
19	24.413049148947366	24.35320918431405	26.59181404873665	24.641927618001933
20	23.1907075592115	24.46959019019894	28.173348540859745	24.166353709729822
21	22.6580636683861	28.099101885508702	27.082518462139355	22.160315983965837
22	23.57087716006404	26.131955264928603	26.4963123047615	23.800855270245854
23	25.69012224390263	24.768762174349632	28.070998398978197	21.470117182769542
24	23.861043222577667	26.350547217615638	27.919554152592884	21.868855407213815
25	24.163551838070276	26.3134403526394	27.697463923046268	21.825543886244056
26	24.564107957196445	26.806600676006674	27.627227233917324	21.002064132879557
27	24.8120480097964	26.15596154948149	27.628145106586345	21.403845334135774
28	25.88778434307012	25.81143595469427	26.305271986873073	21.99550771536253
29	23.502537287359765	26.42224041230169	28.17341041784544	21.901811882493103
30	24.850698241973326	28.05744128705759	27.099496874611255	19.992363596357823
31	25.280617794056205	28.478134483790186	26.01711241370018	20.224135308453437
32	22.881343593252915	27.57151489120069	28.789280956298274	20.757860559248122
33	23.461811709388993	25.026239002168232	30.352405027111683	21.15954426133109
34	23.78418053229415	28.613988463389916	27.999671626789137	19.602159377526796
35	22.563203273418093	27.063872405018703	27.716011594439316	22.656912727123892
36	21.831477862699625	27.440251768298385	27.54040712037783	23.18786324862416
37	23.206914794530757	29.487366342535676	25.106501248090606	22.199217614842965
38	24.044611595924277	28.252109034595385	26.32182090493599	21.38145846454435
39	21.79312035238099	28.934443415144646	26.158367786841225	23.114068445633144
40	21.000918290244794	28.107951525844086	27.49699326225622	23.394136921654898
41	22.066323541750602	29.367612565658735	26.87587258369433	21.690191308896328
42	21.14619808481283	30.05116576280396	26.57890622746984	22.22372992491337
43	22.609370156294432	26.589758066374802	27.12696436467449	23.673907412656277
44	22.306986139159125	28.111959212293108	26.68112150177513	22.899933146772632
45	21.749625535719964	27.049708581309	29.230355115970454	21.970310767000583
46	22.225865796560715	28.904673555572103	27.26838001925031	21.60108062861687
47	22.480697714755827	28.596005397638212	28.65004940939463	20.27324747821133
48	23.05356795820426	27.27027113523181	28.070754806682164	21.605406099881773
49	22.07570201640556	29.189993211468146	25.86323102896729	22.871073743159002
50	21.81946398122479	30.246880348554523	27.179651733311445	20.754003936909243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68639.0
1	73712.5
2	110847.0
3	141560.5
4	131864.5
5	85838.5
6	34756.5
7	24160.5
8	27479.5
9	26839.0
10	23914.5
11	20435.5
12	18014.5
13	16813.0
14	16357.5
15	17407.0
16	19583.0
17	22235.5
18	26289.0
19	31726.5
20	36614.5
21	43503.5
22	52716.0
23	65387.5
24	75088.5
25	85176.0
26	101951.5
27	123307.0
28	143754.5
29	156348.5
30	170270.5
31	202930.0
32	246421.5
33	260942.0
34	277191.5
35	316174.0
36	339023.5
37	360922.0
38	373559.0
39	374902.5
40	386819.5
41	387325.5
42	395527.0
43	435456.5
44	460529.0
45	457491.5
46	467365.0
47	471467.0
48	497936.5
49	547000.5
50	587080.0
51	595914.0
52	555996.0
53	513100.0
54	488283.5
55	460023.0
56	433340.0
57	428316.0
58	428377.0
59	384430.0
60	304079.0
61	249084.5
62	212805.5
63	171098.0
64	131114.0
65	106091.5
66	75397.0
67	49625.0
68	37337.0
69	26090.5
70	17991.5
71	11246.0
72	7571.0
73	6402.5
74	5288.0
75	3486.5
76	2124.0
77	1413.0
78	709.0
79	570.5
80	487.0
81	450.0
82	351.0
83	294.0
84	257.0
85	162.5
86	226.0
87	224.5
88	154.0
89	153.0
90	77.0
91	1.0
92	1.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	28070.0
25	33973.0
26	37065.0
27	43648.0
28	46140.0
29	41731.0
30	44048.0
31	41337.0
32	38622.0
33	32384.0
34	29417.0
35	28197.0
36	29280.0
37	32076.0
38	19926.0
39	19824.0
40	23729.0
41	26305.0
42	36717.0
43	58344.0
44	170296.0
45	440531.0
46	855118.0
47	715366.0
48	1429215.0
49	1006712.0
50	1876091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.869998012899197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.82422198587925	6.891649322528605
2	12.20097053980997	2.408471099667321
3	4.953462111614542	1.4667198359582097
4	2.617701008273844	1.0334681500010827
5	1.5081418322319478	0.7442678443649738
6	1.0652864370121995	0.6308625009887324
7	0.7490240784314628	0.5175006316012534
8	0.5615723198863579	0.4434174145102036
9	0.4671847499338741	0.41500012981537315
>10	3.7013097743685117	7.299333851845305
>50	0.46416970053274825	3.2683547140720406
>100	1.5220381257388074	41.214069845698894
>500	0.3127434525446531	19.33059481709516
>1k	0.04557718579749498	8.438976739961195
>5k	0.004197898691874538	2.551666808848468
>10k+	0.002398799252499736	3.345646293043213
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	25540	0.3555042327831694	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14249	0.19833906863458814	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13436	0.18702250867950918	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	13245	0.18436388266300233	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13048	0.18162173959885647	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	11422	0.15898861968869857	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11108	0.15461789419559302	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	10999	0.15310066783015194	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	8488	0.11814878339324753	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7845	0.10919853978793909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.047159292900132266	0.0	0.0	0.0	0.0
2	0.06809423284163135	0.0	0.0	0.0	0.0
3	0.09113101848204425	0.0	0.0	0.0	0.0
4	0.13663388993733716	0.0	0.0	0.0	0.0
5	0.15784722003763277	0.0	0.0	0.0	0.0
6	0.23514224762748948	0.0	0.0	0.0	0.0
7	0.2617981053322573	0.0	0.0	0.0	0.0
8	0.29850384776957983	0.0	0.0	0.0	0.0
9	0.3433803413675805	0.0	0.0	0.0	0.0
10	0.36888087991334273	0.0	0.0	0.0	0.0
11	0.4011323798099208	0.0	0.0	0.0	0.0
12	0.43569451802451004	0.0	0.0	0.0	0.0
13	0.4670273303970595	0.0	0.0	0.0	0.0
14	0.4962026190389359	0.0	0.0	0.0	0.0
15	0.5312380205234792	0.0	0.0	0.0	4.1758523819479575E-5
16	0.5623202817531119	0.0	0.0	0.0	4.1758523819479575E-5
17	0.6053733198109954	0.0	0.0	0.0	4.1758523819479575E-5
18	0.633407208801806	0.0	0.0	0.0	4.1758523819479575E-5
19	0.664057965285304	0.0	0.0	0.0	4.1758523819479575E-5
20	0.6974508648329478	0.0	0.0	0.0	4.1758523819479575E-5
21	0.7282547359037839	0.0	0.0	0.0	4.1758523819479575E-5
22	0.7537831134654257	0.0	0.0	0.0	4.1758523819479575E-5
23	0.7808147978845689	0.0	0.0	0.0	4.1758523819479575E-5
24	0.8099483280026257	0.0	0.0	0.0	4.1758523819479575E-5
25	0.8371888050408662	0.0	0.0	0.0	4.1758523819479575E-5
26	0.8639838578250323	0.0	0.0	0.0	4.1758523819479575E-5
27	0.890486600942462	0.0	0.0	0.0	4.1758523819479575E-5
28	0.917532204869545	0.0	0.0	0.0	4.1758523819479575E-5
29	0.946651815479662	0.0	0.0	0.0	4.1758523819479575E-5
30	0.97570182855008	0.0	0.0	0.0	4.1758523819479575E-5
31	1.0034016493503348	0.0	0.0	0.0	4.1758523819479575E-5
32	1.0304890118012373	0.0	0.0	0.0	4.1758523819479575E-5
33	1.0584672227602885	0.0	0.0	0.0	4.1758523819479575E-5
34	1.0890762207199671	0.0	0.0	0.0	4.1758523819479575E-5
35	1.1159547905517722	0.0	0.0	0.0	4.1758523819479575E-5
36	1.1404670440338065	0.0	0.0	0.0	4.1758523819479575E-5
37	1.1561404099740513	0.0	0.0	0.0	4.1758523819479575E-5
38	1.1705053421679523	0.0	0.0	0.0	4.1758523819479575E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTCGT	25	2.4900146E-8	155.03299	44
TCCACGA	145	0.0	117.61124	44
TAGCGTG	120	0.0	96.84339	43
TTAACGG	100	0.0	96.00127	43
AATCGCA	230	0.0	90.99763	44
CGATACA	100	0.0	90.94857	43
CCCAACG	185	0.0	90.12922	43
CGATGAC	40	5.838956E-10	88.42222	43
GACGTTC	185	0.0	87.9917	44
ACGTTTG	115	0.0	87.873024	43
GACCGCC	105	0.0	86.61769	43
GCGCGAC	100	0.0	85.895874	43
GCCCGCA	170	0.0	82.076294	44
AATACCG	175	0.0	80.84318	43
CCGTGAC	240	0.0	80.74635	44
CCACTCG	135	0.0	74.85479	43
CATGCCG	110	0.0	73.4938	43
CCGCAAC	380	0.0	71.39677	44
GACGATG	80	0.0	69.4746	43
ACGGTTT	165	0.0	67.61634	42
>>END_MODULE