FastQCFastQC Report
Tue 24 May 2016
ERR841106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975394
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA104540.26296764547111556No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG80030.20131337925247159No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75480.18986797283489384No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC73420.18468609652275975No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT72850.18325227637814012No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG72010.18113927827027962No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.1729891427113891No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC68650.1726872858388376No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA67290.16926624128325393No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT59080.14861420025285543No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58540.14725584432637367No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT55990.1408413857846543No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT53850.13545827155748588No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGA52460.13196176278376434No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT49180.12371100826735663No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT47940.12059182058432447No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA47460.11938439309411848No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT43380.10912125942736746No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGT42540.10700826131950696No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTT41960.10554928643550802No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.1035369072851647No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT40600.10212824187992436No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC40200.10112205230475269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT600.0195.6811844
TATATCC550.0195.6811844
TAGGCGT351.8189894E-12195.6811844
ACGGATG900.0195.6811744
CGAATAT800.0195.6811744
ACGCTCC950.0195.6811744
TCGCCCC1400.0174.7153544
CGCCCAT1250.0172.1994344
GCCACGG1000.0156.5449544
AGATCCA1700.0149.6385544
TATCCGG201.1721849E-4146.7608844
CCCGATG201.1721849E-4146.7608844
ATGACGC354.620415E-8139.7722844
ACGGCAG600.0130.4541244
ATCGGCA1050.0130.4541244
TGACCAC1650.0124.5243944
TTTGACG502.8267095E-9117.40871444
ATCGGCC252.8501148E-4117.40871444
ACTACGG1850.0111.0622944
CAGGCCT6000.0109.2553244
Produced by FastQC (version 0.11.5)