##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841106.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3975394
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.6143557091448	39.0	38.0	39.0	35.0	39.0
2	38.02808903972789	39.0	38.0	39.0	35.0	39.0
3	38.12824213147175	39.0	38.0	39.0	35.0	39.0
4	38.70023474402789	39.0	38.0	40.0	35.0	41.0
5	39.33833325703063	40.0	39.0	41.0	36.0	41.0
6	39.321119617325984	40.0	39.0	41.0	36.0	41.0
7	39.32693614771265	40.0	39.0	41.0	36.0	41.0
8	39.26589943034577	40.0	39.0	41.0	36.0	41.0
9	39.23721724186332	40.0	39.0	41.0	36.0	41.0
10	39.20514520070212	40.0	39.0	41.0	35.0	41.0
11	39.166922825762676	40.0	39.0	41.0	35.0	41.0
12	39.04931913666922	40.0	38.0	41.0	35.0	41.0
13	38.91083978091228	40.0	38.0	41.0	35.0	41.0
14	38.95345291561038	40.0	38.0	41.0	35.0	41.0
15	38.94916126552488	40.0	38.0	41.0	35.0	41.0
16	38.986849102252506	40.0	38.0	41.0	35.0	41.0
17	38.948515291817614	40.0	38.0	41.0	35.0	41.0
18	38.95790379519615	40.0	38.0	41.0	35.0	41.0
19	38.92832458870743	40.0	38.0	41.0	35.0	41.0
20	38.99799365798711	40.0	38.0	41.0	35.0	41.0
21	38.99185766241032	40.0	38.0	41.0	35.0	41.0
22	39.007475233901346	40.0	38.0	41.0	35.0	41.0
23	38.96212677284314	40.0	38.0	41.0	35.0	41.0
24	38.93725930058756	40.0	38.0	41.0	35.0	41.0
25	38.87901723476047	40.0	38.0	41.0	35.0	41.0
26	38.80163738817089	40.0	38.0	41.0	35.0	41.0
27	38.81351624178794	40.0	38.0	41.0	35.0	41.0
28	38.80008988833104	40.0	38.0	41.0	35.0	41.0
29	38.59026031385021	40.0	38.0	41.0	35.0	41.0
30	38.651558239038096	40.0	38.0	41.0	35.0	41.0
31	38.48663459691299	40.0	37.0	41.0	34.0	41.0
32	38.42061659049536	40.0	37.0	41.0	34.0	41.0
33	38.46160776340522	40.0	37.0	41.0	34.0	41.0
34	38.46672594111935	40.0	37.0	41.0	34.0	41.0
35	38.38547821197563	40.0	37.0	41.0	34.0	41.0
36	38.3833381313571	40.0	37.0	41.0	34.0	41.0
37	38.31653392295402	40.0	37.0	41.0	34.0	41.0
38	38.293775030655866	40.0	37.0	41.0	34.0	41.0
39	38.192849180238724	40.0	36.0	41.0	34.0	41.0
40	38.17730065600531	40.0	36.0	41.0	34.0	41.0
41	38.09417198985985	39.0	36.0	41.0	34.0	41.0
42	37.98308547682718	39.0	36.0	41.0	34.0	41.0
43	37.897581592649615	39.0	35.0	41.0	34.0	41.0
44	37.82187096873165	39.0	35.0	41.0	34.0	41.0
45	37.60309005692968	39.0	35.0	41.0	33.0	41.0
46	37.31905924503318	39.0	35.0	41.0	33.0	41.0
47	37.011896595358635	38.0	35.0	40.0	32.0	41.0
48	37.055037530460424	38.0	35.0	40.0	32.0	41.0
49	36.93367975380654	38.0	35.0	40.0	32.0	41.0
50	36.77846416344656	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	11.0
22	40.0
23	70.0
24	181.0
25	366.0
26	765.0
27	1810.0
28	3836.0
29	8348.0
30	16887.0
31	30913.0
32	53770.0
33	94088.0
34	175778.0
35	163410.0
36	254416.0
37	425775.0
38	568074.0
39	915750.0
40	1261101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.27742860204548	18.92524363622826	32.79732776172626
2	24.614918672212113	19.09632101874682	32.442595626999484	23.84616468204158
3	21.32357195286807	20.212663197660408	33.06077334724558	25.40299150222594
4	20.496131955725648	21.18501964836693	37.90099798913013	20.41785040677729
5	17.68219703506118	22.807198481458695	36.66783720053912	22.84276728294101
6	19.254167008351878	24.734101827391196	33.52168363689234	22.490047527364585
7	23.570166881571993	24.29464853043497	28.378897789753672	23.75628679823937
8	24.846920833507323	23.84367436284303	30.23627343604181	21.07313136760784
9	25.341689402358607	22.2940921075999	28.5206195914166	23.84359889862489
10	21.968967101122555	20.87310088006371	32.11098069776229	25.04695132105145
11	27.678640154912948	22.862664681790033	25.959137635162705	23.499557528134318
12	26.770327670666106	23.11765827487791	28.984221438176945	21.127792616279038
13	25.737876547582456	22.179864436078535	28.008569716611735	24.07368929972727
14	21.914331007190736	23.14859860431444	30.18971201345074	24.747358375044083
15	25.50572345785097	26.40279177359527	28.581896536544555	19.509588232009204
16	25.51998619507903	28.396430643101034	25.49890652347918	20.584676638340753
17	23.816431780095257	27.934489009139725	25.83391734253259	22.41516186823243
18	22.816656663465306	25.024764840918913	26.36272027376406	25.79585822185172
19	23.92821944189683	23.15866050006616	26.848760148050733	26.06435990998628
20	25.126113285878077	23.19875715463675	27.923395769073455	23.751733790411716
21	23.894939721698023	23.98491822445775	27.35942651218973	24.760715541654488
22	25.376704799574583	24.48330907577966	27.441908902614433	22.698077222031326
23	27.696751567266038	25.463463495693762	26.994632481711246	19.845152455328957
24	27.691142060384454	26.971968061530504	24.90407240137707	20.432817476707967
25	28.028357545511483	26.293970864566706	24.9193825389449	20.758289050976906
26	27.527736054385894	27.493836150634237	23.70850748130561	21.269920313674263
27	26.29278051647556	25.469702657043335	25.602455507549827	22.635061318931275
28	26.21755793123671	25.215418281647544	25.266636703889656	23.300387083226095
29	25.350855106128513	27.026523614096533	25.17153202945945	22.451089250315512
30	28.12401564077083	26.459160930247037	26.10451916565258	19.312304263329555
31	26.125584319014333	27.377350181062116	25.93319193368592	20.563873566237632
32	23.819362222852234	27.924381323976423	27.029997417416503	21.226259035754843
33	24.845765682407787	26.207818138220418	29.404034891494735	19.542381287877063
34	25.358180020750492	27.089258229782565	27.14947242102273	20.40308932844421
35	21.42624179117634	27.21906356971807	28.050944457132477	23.30375018197311
36	24.971392957073473	24.207474238905096	26.588063142887115	24.23306966113432
37	23.823902923883303	30.317258910610366	24.951811229385015	20.907026936121312
38	26.74602963878911	25.381403391083218	25.049675234775425	22.82289173535224
39	21.875771500040784	29.42836944995786	26.00459501345877	22.69126403654259
40	21.56101875049102	28.263277233167766	26.914330857127894	23.261373159213317
41	23.58262879909802	28.047567457458943	26.384119211053076	21.985684532389957
42	22.552212019689268	30.345469186732004	24.45490349124509	22.647415302333634
43	22.719224465029196	27.238720601612126	27.933483574260393	22.10857135909829
44	24.791681484301677	29.540883423912668	24.95942408559503	20.70801100619063
45	21.852260414336108	28.559314298416787	26.919062300147917	22.669362987099184
46	22.867955790464713	27.82329572380375	26.363441478753195	22.945307006978343
47	22.99109897921923	27.129306344472397	27.966778019463362	21.91281665684501
48	24.866961003956003	25.526473610341426	26.391668054699046	23.214897331003524
49	20.43290228710197	30.36026950448504	24.810792639635746	24.39603556877725
50	21.586876882647633	27.257004581389744	29.815875378365508	21.340243157597115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24540.0
1	27687.5
2	48363.0
3	65129.5
4	60764.0
5	39268.0
6	15488.5
7	10436.5
8	11785.0
9	12495.0
10	12353.5
11	10881.0
12	8689.5
13	6621.0
14	5833.5
15	6387.5
16	6961.5
17	8333.0
18	11091.5
19	15052.5
20	18142.5
21	19912.0
22	22122.0
23	25419.0
24	27432.5
25	30561.5
26	39686.0
27	55261.5
28	68892.0
29	75222.0
30	81278.0
31	94369.0
32	115964.5
33	136415.0
34	154798.5
35	165380.5
36	157333.0
37	145908.5
38	148926.5
39	168409.5
40	189314.0
41	206212.5
42	217951.5
43	222050.5
44	230564.0
45	234902.5
46	254473.0
47	289902.0
48	322535.0
49	350093.0
50	362868.0
51	348178.0
52	321596.0
53	310919.5
54	304758.0
55	297532.0
56	300096.0
57	305504.5
58	302297.5
59	263987.5
60	195123.0
61	147824.0
62	122130.0
63	95905.5
64	76094.0
65	63354.5
66	40946.0
67	23300.0
68	17846.5
69	14766.5
70	11811.0
71	7976.0
72	4749.5
73	3038.5
74	2297.5
75	1620.5
76	1052.0
77	795.5
78	580.0
79	455.0
80	338.5
81	172.5
82	73.0
83	58.0
84	49.5
85	36.0
86	32.0
87	24.0
88	15.0
89	15.5
90	11.5
91	4.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15954.0
25	15476.0
26	17118.0
27	22001.0
28	28601.0
29	22214.0
30	27995.0
31	23641.0
32	24052.0
33	18431.0
34	16226.0
35	16456.0
36	16821.0
37	19434.0
38	13074.0
39	12076.0
40	13411.0
41	13883.0
42	19454.0
43	33821.0
44	112374.0
45	305510.0
46	437010.0
47	490980.0
48	698479.0
49	723901.0
50	817001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.90150472368779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69641562032277	9.636961589681405
2	10.887509325990804	2.8093050599693044
3	4.1476156499617085	1.6053144870006715
4	2.1414388172753367	1.105111320662646
5	1.2843446076255483	0.8284989011061977
6	0.8468486740019331	0.655537330073081
7	0.612342366796423	0.5530096556416748
8	0.46377195458188575	0.4786684850201694
9	0.35229470142217933	0.40906185790755833
>10	2.813992929500329	7.336906846115804
>50	0.3729174097753197	3.40872974087394
>100	1.1056081021337765	37.937174317884256
>500	0.2275810155886782	18.442238474968924
>1k	0.04206117779856758	10.12783339699793
>5k	0.004882100994476594	4.159140583571397
>10k+	3.7554623034435344E-4	0.5065079525250505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10454	0.26296764547111556	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG	8003	0.20131337925247159	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7548	0.18986797283489384	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	7342	0.18468609652275975	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7285	0.18325227637814012	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	7201	0.18113927827027962	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6877	0.1729891427113891	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	6865	0.1726872858388376	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	6729	0.16926624128325393	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	5908	0.14861420025285543	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5854	0.14725584432637367	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT	5599	0.1408413857846543	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5385	0.13545827155748588	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGA	5246	0.13196176278376434	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT	4918	0.12371100826735663	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT	4794	0.12059182058432447	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	4746	0.11938439309411848	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	4338	0.10912125942736746	No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGT	4254	0.10700826131950696	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTT	4196	0.10554928643550802	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4116	0.1035369072851647	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	4060	0.10212824187992436	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4020	0.10112205230475269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03803396594148907	0.0	0.0	0.0	0.0
2	0.05695032995471644	0.0	0.0	0.0	0.0
3	0.07682257406435689	0.0	0.0	0.0	0.0
4	0.12459142414563186	0.0	0.0	0.0	0.0
5	0.1432813955044456	0.0	0.0	0.0	0.0
6	0.2367815617772729	0.0	0.0	0.0	0.0
7	0.2609804210601515	0.0	0.0	0.0	0.0
8	0.2980333521658482	0.0	0.0	0.0	0.0
9	0.34250693138843596	0.0	0.0	0.0	0.0
10	0.36919610986986445	0.0	0.0	0.0	0.0
11	0.4039599596920456	0.0	0.0	0.0	0.0
12	0.43573039552809106	0.0	0.0	0.0	0.0
13	0.4664946417889648	0.0	0.0	0.0	0.0
14	0.4976613638799072	0.0	0.0	0.0	0.0
15	0.5370788404872574	0.0	0.0	0.0	0.0
16	0.5699309301166123	0.0	0.0	0.0	0.0
17	0.6161150316169919	0.0	0.0	0.0	0.0
18	0.6439613281098678	0.0	0.0	0.0	0.0
19	0.6741721701043972	0.0	0.0	0.0	0.0
20	0.7110238632950595	0.0	0.0	0.0	0.0
21	0.7493848408484794	0.0	0.0	0.0	0.0
22	0.7764764951599766	0.0	0.0	0.0	0.0
23	0.8026625788538193	0.0	0.0	0.0	0.0
24	0.8291002099414548	0.0	0.0	0.0	0.0
25	0.8558900073804006	0.0	0.0	0.0	0.0
26	0.8899746792393408	0.0	0.0	0.0	0.0
27	0.9173430356840102	0.0	0.0	0.0	0.0
28	0.9444346899955074	0.0	0.0	0.0	0.0
29	0.9730104739303828	0.0	0.0	0.0	0.0
30	1.0004291398538108	0.0	0.0	0.0	0.0
31	1.028828840613031	0.0	0.0	0.0	0.0
32	1.0541596631679777	0.0	0.0	0.0	0.0
33	1.0861313369190575	0.0	0.0	0.0	0.0
34	1.1142291808057265	0.0	0.0	0.0	0.0
35	1.142477953128671	0.0	0.0	0.0	0.0
36	1.1677081567261005	0.0	0.0	0.0	0.0
37	1.1845366773708468	0.0	0.0	0.0	0.0
38	1.1990761167320774	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	60	0.0	195.68118	44
TATATCC	55	0.0	195.68118	44
TAGGCGT	35	1.8189894E-12	195.68118	44
ACGGATG	90	0.0	195.68117	44
CGAATAT	80	0.0	195.68117	44
ACGCTCC	95	0.0	195.68117	44
TCGCCCC	140	0.0	174.71535	44
CGCCCAT	125	0.0	172.19943	44
GCCACGG	100	0.0	156.54495	44
AGATCCA	170	0.0	149.63855	44
TATCCGG	20	1.1721849E-4	146.76088	44
CCCGATG	20	1.1721849E-4	146.76088	44
ATGACGC	35	4.620415E-8	139.77228	44
ACGGCAG	60	0.0	130.45412	44
ATCGGCA	105	0.0	130.45412	44
TGACCAC	165	0.0	124.52439	44
TTTGACG	50	2.8267095E-9	117.408714	44
ATCGGCC	25	2.8501148E-4	117.408714	44
ACTACGG	185	0.0	111.06229	44
CAGGCCT	600	0.0	109.25532	44
>>END_MODULE