FastQCFastQC Report
Tue 24 May 2016
ERR841105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864543
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT54760.6333982231074683No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT35890.4151326191988137No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT29490.34110506938347773No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT27630.31959081271839573No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG24000.27760331180750986No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA23940.2769093035279911No Hit
TTTCAGGATACATGTGCCGAATGTGCAGGTTTGTTACATAGGTATATAT21360.2470669475086838No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGT20030.23168309731268427No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC18950.21919094828134633No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG18600.21514256665082013No Hit
CCTCTTTTTCCGGCTGGAACCATGGAGGG18250.21109418502029395No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.2061204590170761No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.2054264507375573No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.20288175371265513No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG17480.20218774543313633No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC17020.19686701529015907No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT15590.18032648462829495No Hit
CTTTTTCCGGCTGGAACCATGGAGGG15580.18021081658170848No Hit
ATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGA14900.17234538941382904No Hit
TCTGCAACACCGCCTAGACCGACCGGATACACGGATGCTTGATGGAGTA14250.16482696638570898No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG14230.16459563029253607No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA14180.16401729005960375No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA14060.1626292735005662No Hit
CCAGTTCCGTCTGTGTGTTCGAGTGGACAAAATGGCGAAGATCGCCAA13700.15846522382345354No Hit
ATCATTGGGAGTAGGACCCACAGTAGATCTGGGTGGGCTCTGAGGAAGCT13580.157077207264416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA13480.15592052679855137No Hit
AGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATT13380.15476384633268675No Hit
ATCTAAATTTTGCTTAGTCTACATCTCACTTGCTTATACTATTCTCTCCA13340.1543011741463409No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.1527974895407169No Hit
TGGAGAACTATGAGGCATCACAATAGAAGGGCCAGAAAAGACTGATTA13150.1521034812611981No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC13100.15152514102826578No Hit
ATTGTAGAGCATGTCGCTTGAAGTCTACTTGTATCTACGGTGGTGCTCCT13010.15048412860898763No Hit
CGCTGAGGAAAAATAACTAAACATGGGCAAAGGAGATCCTAAGAAGCCGA12730.1472454233045667No Hit
ATTCAAAGTGTCATTAAAGGGTTGTATAGGATATCATGAAAAATAGATGA12700.1468984191648073No Hit
CAGCCCACTGCTGCTTATGAGTATTATGATGCTGGCAATCACTGGTGCAA12640.14620441088528852No Hit
AGGAACTCTTCACATGTGTCTCAGAGCAGTTTACAGCAATGTTCAGGCGC12530.14493206237283743No Hit
CCCAGCTTGTCCTCTATGACTTACCCAGAAGGCAACGCTTCTCTTTCTG12460.14412238604673222No Hit
TTTCAGGATACATGTGCCGAATGTGCAGGTTTGTTACATAGGTATAT12300.1422716973013488No Hit
ATGTGATGGGATATCACCCTGAAGAGTTGTGTCTTTTGTGGCCAGTGACA12280.14204036120817587No Hit
ACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAA12190.14099934878889772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA12130.14030534050937896No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGGC12090.1398426683230331No Hit
ATAATCCTATTTATTACCTCAGAAATTTTTTTCTTCGCAGGATTTTTC11950.1382233156708226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA11940.13810764762423614No Hit
CCCCCTGCCTTAATAAACCAAATATCCCAAACAGAATTTAACATTATAT11900.1376449754378903No Hit
AGAGGAGGAAGGATTAGAAGATATTGACGAAGAAGGGGATGAGGATGAAG11840.13695096715837154No Hit
AACTACAGGGTACTTGAGCCTTTCCCCTCAAGTGCATTCGAAGTCACCCA11820.1367196310651986No Hit
ACCTTCAGAAAAGGCTTATGAGAAGCCACCAGAGAAGAAGGAAGGAGAGG11770.13614129083226628No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG11770.13614129083226628No Hit
AAGAAAAATTCTTCTGCCTTGGGATCCTGTTGATCTGTGACCTTATCCCC11730.13567861864592046No Hit
CTGAGGAGGCCAAGTACAAGTTGTGCAAAGTGAGAAAGATCTTTGTGGGC11630.1345219381800558No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC11480.1327869174812589No Hit
AGCATTCACATTTTTGTGCAGCGTCGCCCCTGCCCATCTCCAGAACCTT11470.13267124943467243No Hit
ACAAGTCCTTCTTGATCCTGAACTGGGTTAGGTGCCGCTGTTGCTGCTC11450.1324399133414995No Hit
TTATTGCTCATTTAAAACTGTTAAGTCCAGTATAATTTTCCCTGATATG11370.13151456896880778No Hit
CTGATTTGATCTTTTGCCGCAAGCAGGCTGG11330.13105189678246193No Hit
ACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGCTACCCC11320.1309362287358755No Hit
CTCTTCATGTTGTAACAAAAAGGAAAAAAGAAAAAAAAATCCCATCCCTT11220.12977954827001087No Hit
AAAGCCACCAAGATGCTGACATTGAAGACTTCAAAGCTAAGAAGAAGGA11210.1296638802234244No Hit
CACAAATCCTTGAGCTATGCATAGGCAGCTCACAAATCACCTCTGTCTGG11070.12804452757121393No Hit
AGAGGTCTGATTGAGAATCAGACTGTTGATTGGAGAAGAGAGACACAA11050.127813191478041No Hit
ACCAGTTTACTGGAGTTGGAATTAGGGTGTCATTTACAGGAAAACAAGG11040.12769752343145452No Hit
CTAACTTGGGCCAGAAACCAAAGCTGAGCTTTTAACTTTCTCCCTCTATG11010.12735051929169516No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT10970.1268878471053493No Hit
AGGTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAG10960.12677217905876284No Hit
ACACGAGAACATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAA10950.12665651101217637No Hit
TGGGTAGTGCTTTCTTTCTCTAGTTGGGCAGGAGCTGTTCCTGTGGAAA10880.12584683468607114No Hit
CGGAACGTCAAGAACTGGAGGTTTGTGCAATTTGAGACCGGTCGGCACTG10860.12561549859289822No Hit
ATTTGGCCAAGAGACGAATGTCCCTTTGAACAACTTCAGTGCTGATCAGG10860.12561549859289822No Hit
TACCGTAGGAAATATTTTAGAATGCCTAAAGGGTTAAATAATAAGTGGGA10820.12515282640655237No Hit
CTGCCATGGTAACATGCACATCCTGTTTACACCTTCATCTGGGCAAGTT10800.12492149031337943No Hit
ATTCCTATTATTCCCATGGTGCTGATAAATGGTGCTGAAGGAATCGGTAC10760.12445881812703358No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA10640.12307080156799605No Hit
CGGCAAGATGGACGCGGGATTTTTCCGCGGAACAAGTGCAGAACAGGA10540.12191412110213141No Hit
TTTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC10530.12179845305554494No Hit
TTCCTGAGTTGAGAAGACAGACTGCTGAAATGTTTCCAGGCAGACCATG10500.12145144891578556No Hit
CTATTATTTGAGGCTAAAAGTTGATGTGTGACAAAATACTTATGTGTTGT10440.1207574406362668No Hit
CTCAAAACAAAGCGGTGTGATATTCCTGCCATGTATAATAATCTGGATGT10420.12052610454309387No Hit
CTCCCAAGTAGCTGGGATTACAGGCGTGTACCACTACACCTGGCTAATTT10360.11983209626357509No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA10340.11960076017040217No Hit
ATTTTCAGGAATTCACGAAGACCGTGAAAATGAATGAAATAGCTCTTTTA10320.11936942407722924No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA10310.11925375603064278No Hit
CAACAGGACTACCCCAAGAACCCATGTGCTCTCCCGAGTAACCCAGATGG10240.11844407970453753No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA10200.11798140751819168No Hit
AGTCTATTTGGTATTGGAGAGTAAGGGTGGATTTTGCTATGTCTTGTAGG10180.11775007142501875No Hit
ATGCAATGAAAATTGTTTTCCTCAATACTGACCCCTCTATTGTAATGACT10120.1170560631455No Hit
ATGAACTAATCATGAACATTCACTTTGAATAGCAGGGCTCTCTTCATCCT10040.1161307187728083No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA10040.1161307187728083No Hit
CTCATAATCAATGCCATGTACATTAAGATCTGCGAAAGACCAACTTTTAG9900.11451136612059781No Hit
ATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTC9860.11404869393425196No Hit
CCCGGAACTTCCTAGACGCCGTACGTGCCAGATGGTGTTACCTGGAGCT9850.11393302588766552No Hit
AGCCTCGCCTTAAGCCAGTCTTCATTCGAAAGAAGGACCGAGTGACAG9840.11381735784107905No Hit
TCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA9830.11370168979449259No Hit
CAAATCTGGTAGGGTCAGTTTTTCTGTAATGTTAAATATGCAGGACTGGT9830.11370168979449259No Hit
AAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTTAC9830.11370168979449259No Hit
CATGCCAGCAGTGGTCATATTGAGTGCCAAAGAGAAGAGCTTACTGGGTA9820.11358602174790612No Hit
TCTGCAACACCGCCTAGACCGACCGGATACACGGATGCTTGATGGAG9760.11289201346838734No Hit
CCTTTCTAACTCCGCTGCCGCCATGGCTCCTGTGAAAAAGCTTGTGGTG9750.11277634542180089No Hit
ATGTGCTGTGTCCACTCAGGGTTAAATGGATTAAGGGCGGTGCAAGATGT9650.11161966495593625No Hit
TAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCG9600.11104132472300393No Hit
ATGTTGAATGATGCTTTAACACATTCTGCAGTTTTAATTCAGGGGCATG9580.110809988629831No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG9500.10988464425713933No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA9470.10953764011737993No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC9440.10919063597762055No Hit
ATCGTAGACCATATGAAAAGGGTCGCCTAATCATCGAATTTAAGGTAAAC9430.10907496793103408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA9420.10895929988444762No Hit
AGAGCAGATAAGAAACCAGCTGACAGCCATGTCCTCAGTACTTGCAAAAG9400.1087279637912747No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG9380.10849662769810177No Hit
CTGCAAATGTTTCCTGGGGTGACTTAACAAGGAATAATCCCACAATATAC9350.10814962355834237No Hit
TACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT9340.10803395551175592No Hit
ATTGCCAGGAGCCTCGAGGACCGACGGAAGCTGTTGGCTATTTTCTTTAT9260.10710861113906424No Hit
AGAGCAGGAACCTAATATGCTTCTTGAATCTGAAGATGAGCTACTTGTAG9230.10676160699930484No Hit
CAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAA9170.10606759871978606No Hit
TTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATAATGATG9160.1059519306731996No Hit
ATCTGTAATCCCAACACTTTGAGAGGGCAAGGCAGGAGAATCACTTGAGC9160.1059519306731996No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.1059519306731996No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCA9110.10537359044026728No Hit
CAAGCAATCCTACTGCCTCAGACTCCAAAGTAGCTAGGATTATAGACATG9070.10491091825392143No Hit
AAAGGGCTCGTGACAGAGAAAGAAGAAAGAGAAGTCGTTCACGAAGTAG9070.10491091825392143No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT9060.10479525020733498No Hit
AGATTTGTGGTGCGTTCTGAGCCGTCTGTCCTGCGCCAAGATAAAAGAA8990.10398557388122973No Hit
ATTGTGGGTTATTATGCCACATCTCCATGAAAATGCATACGTTAATGAAC8990.10398557388122973No Hit
CATAGTCTTCAGATAACAGACAGTGAACATCCTTGACTCGATGAAAGTTA8940.10340723364829743No Hit
CATCGGCTAATAGCCCGGAGACAAGCGTAATGAAGCAAATAAGCAACA8930.10329156560171097No Hit
ACAACCTTAGCTAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT8920.1031758975551245No Hit
CAGGTCCCTCCAACCACCAGCACTGACTCCTGGGCTCTGAAGAATCACAG8910.10306022950853805No Hit
AGAGTGGATCAGCTTCAGGCCCAGCTGTCAACTCAAGAGAAAGAAATGGA8870.1025975573221922No Hit
AATTCATAATAGAGAAACCCTACAAATGTGAAGAACGTGGCCTGGCTT8850.10236622122901927No Hit
AACTATTAATATATTCCTGTAAAGCCTTAATGTCACTGAAAAAATTCAGT8830.10213488513584634No Hit
CGGGAGAAAAGACTGAAAGAAAGGGAAGCCAGAAGAGAAGCCAACAAGAG8750.10120954076315464No Hit
CCTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG8730.10097820466998171No Hit
CTGTAGTGTAATGTTCAAGCTCAGAAATGCCTTATGTGGATCGTCAGAAT8710.10074686857680878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAG600.0165.3167144
AGCTTAA850.0165.3167144
GACCCCG600.0165.3167144
AATATCA157.2862094E-5165.3167144
TCAATGT850.0165.3167144
CTCATAG1700.0165.3167144
GTCCGCG1000.0165.3167144
GAGTTAG500.0165.3167144
AACTCTG500.0165.3167144
CTTTACG950.0165.3167144
ACATCAA302.6011548E-10165.3167144
TCGGTAC1550.0165.3167144
TATCCCC1150.0165.3167144
CGTATGT201.1070424E-6165.316744
GTATTGC550.0165.316744
GAATGCA800.0165.316744
TGTGGGC1100.0165.316744
GTTAGTA100.0047880406165.316744
TTCAAAC100.0047880406165.316744
CTTATTG750.0165.316744
Produced by FastQC (version 0.11.5)