FastQCFastQC Report
Tue 24 May 2016
ERR841104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742742
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC578742.110078162656203No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT359981.3124821802415247No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA336701.2276036171101765No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC197920.7216136260720112No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT173750.6334901350546278No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA167590.6110308588995975No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA154190.5621746412896291No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT134980.49213524276071174No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA107290.3911778796547397No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG103640.3778700293356065No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA94590.3448738525169338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA93150.3396236321170566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA90890.3313837028783604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA85430.3114766171954927No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC85020.3099817627760832No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC75490.27523551249078476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70840.25828167578284794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA70330.25642222272455817No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC66030.2407444812527026No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT58990.21507673707552516No Hit
AAAAAAAAAAAAAAAAAAAAAAAA55080.200820930295303No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA51820.18893501466780324No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC49400.1801117276068985No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG49010.17868979291526507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46830.1707415425876732No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT44910.1637412487211703No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT44460.16210055484620867No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG43800.15969420382959826No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA40440.14744368956321813No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGAC39090.14252160793833324No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC39020.142266388891117No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA37770.13770890590511248No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG36660.13366186101354047No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC36320.13242222564134723No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA36200.1319847072746908No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT35750.13034401339972917No Hit
ACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATA33660.12272390184712963No Hit
AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA30940.1128068188695838No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT30460.11105674540295807No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT30310.11050984744463753No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC29840.10879623384189982No Hit
CACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG29760.10850455493079553No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC29690.10824933588357928No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC28490.10387415221701495No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT28030.10219699847816528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA100.006422542149.8786544
CGTGCGC100.006422542149.8786544
CAAATGC201.8067276E-6149.8786544
CGTCGTA100.006422542149.8786544
CGAAGGT700.0149.8786544
AGGTCGA100.006422542149.8786544
TTTAGGC1250.0149.8786344
GTCGCGC305.16593E-10149.8786344
TATATCA750.0149.8786344
GGTCATG1200.0149.8786344
GGCGGTC1050.0149.8786344
TGTCTAT2100.0146.3100944
ATTGCAC1200.0143.633744
GAGTCTG1100.0143.0659844
CCACCCG800.0140.5112344
GTCATGC700.0139.1730244
CCGGGCC450.0133.2254644
GGCAAGT1150.0130.3292544
TGCTCAG750.0129.8948244
AAGTCCA750.0129.8948244
Produced by FastQC (version 0.11.5)