##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841104.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742742 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.732780188585 39.0 38.0 39.0 35.0 39.0 2 37.99784886803061 39.0 38.0 39.0 35.0 39.0 3 38.20047966596931 39.0 38.0 39.0 35.0 39.0 4 38.608526795447766 39.0 38.0 40.0 35.0 41.0 5 39.49233249062435 41.0 39.0 41.0 36.0 41.0 6 39.44022478235284 41.0 39.0 41.0 36.0 41.0 7 39.43905442072204 41.0 39.0 41.0 36.0 41.0 8 39.40265398641214 41.0 39.0 41.0 36.0 41.0 9 39.362963049386344 41.0 39.0 41.0 36.0 41.0 10 39.392997226862754 41.0 39.0 41.0 35.0 41.0 11 39.33662407911499 41.0 39.0 41.0 35.0 41.0 12 39.324110324631334 41.0 39.0 41.0 35.0 41.0 13 39.246120123584355 40.0 39.0 41.0 35.0 41.0 14 39.29379941678802 40.0 39.0 41.0 35.0 41.0 15 39.226493778853424 40.0 39.0 41.0 35.0 41.0 16 39.22931795991019 40.0 39.0 41.0 35.0 41.0 17 39.29231695872233 41.0 39.0 41.0 35.0 41.0 18 39.2580421344771 41.0 39.0 41.0 35.0 41.0 19 39.20451285611261 41.0 39.0 41.0 35.0 41.0 20 39.194389410305455 41.0 39.0 41.0 35.0 41.0 21 39.29966981947263 41.0 39.0 41.0 35.0 41.0 22 39.33496223851897 41.0 39.0 41.0 35.0 41.0 23 39.29605810535588 41.0 39.0 41.0 35.0 41.0 24 39.21969911861925 41.0 39.0 41.0 35.0 41.0 25 39.218018752757004 41.0 39.0 41.0 35.0 41.0 26 39.220272383791084 41.0 39.0 41.0 35.0 41.0 27 39.26462787897933 41.0 39.0 41.0 35.0 41.0 28 39.229188730963706 41.0 39.0 41.0 35.0 41.0 29 39.2820988492679 41.0 39.0 41.0 35.0 41.0 30 39.36574623348955 41.0 39.0 41.0 35.0 41.0 31 39.35086858132989 41.0 39.0 41.0 35.0 41.0 32 39.28511653118209 41.0 39.0 41.0 35.0 41.0 33 39.13717874166815 41.0 39.0 41.0 35.0 41.0 34 39.138278395910596 41.0 39.0 41.0 35.0 41.0 35 39.20307149313203 41.0 39.0 41.0 35.0 41.0 36 39.244274571754154 41.0 39.0 41.0 35.0 41.0 37 39.20059349648424 40.0 39.0 41.0 35.0 41.0 38 39.15380235894345 40.0 39.0 41.0 35.0 41.0 39 39.15405106982534 40.0 38.0 41.0 35.0 41.0 40 39.12325375766525 40.0 38.0 41.0 35.0 41.0 41 39.067499078864934 40.0 38.0 41.0 35.0 41.0 42 38.98242656097234 40.0 38.0 41.0 35.0 41.0 43 38.92246207891498 40.0 38.0 41.0 35.0 41.0 44 38.83386311756442 40.0 38.0 41.0 35.0 41.0 45 38.68098672683095 40.0 38.0 41.0 35.0 41.0 46 38.387027904346716 40.0 37.0 41.0 34.0 41.0 47 38.23370442888873 40.0 37.0 41.0 34.0 41.0 48 38.00527099491171 40.0 37.0 41.0 34.0 41.0 49 38.37031877032133 40.0 37.0 41.0 34.0 41.0 50 37.18387596941251 38.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 17.0 21 38.0 22 97.0 23 189.0 24 284.0 25 468.0 26 763.0 27 1450.0 28 2698.0 29 5097.0 30 9592.0 31 17237.0 32 30746.0 33 55379.0 34 104672.0 35 86916.0 36 115409.0 37 185127.0 38 277541.0 39 540412.0 40 1308607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 46.04705801712301 15.675590339886142 38.27735164299085 2 19.897642578120728 30.987748756536345 30.76960939089422 18.34499927444871 3 22.62837700374297 26.57187588187296 22.225167369005177 28.57457974537889 4 16.626572969677788 32.62078606008148 24.285003839223666 26.467637131017064 5 14.514234295460529 36.07984272673113 22.02369016115989 27.38223281664845 6 14.970565951883188 34.447680459919304 24.353876522108166 26.227877066089338 7 18.04147090758081 32.63154171992845 19.16819008131279 30.15879729117795 8 17.464894620055404 34.409434062700754 26.10026754248121 22.025403774762626 9 19.49158907399967 33.217670491792525 26.124586271694533 21.16615416251328 10 23.82972951885376 25.71375652540414 24.81421876355851 25.64229519218359 11 16.730848180397572 29.637457697442926 23.380908594392036 30.250785527767466 12 16.196310115935077 29.050709107892757 26.58868387912534 28.164296897046825 13 15.864561814417835 31.797157734850746 23.932728634337465 28.40555181639396 14 13.519572748731015 31.245228315313657 23.52014881458045 31.715050121374887 15 14.350675346058797 37.141335203967415 27.08216813684991 21.425821313123876 16 13.531239905175186 41.62928922953745 21.70546846914511 23.134002396142257 17 16.146943460230677 43.99334680403771 18.817227431526554 21.042482304205063 18 16.817075758492777 30.906880778432676 22.986704546034588 29.289338917039952 19 17.724744069985437 28.025202516313968 23.299931236696708 30.95012217700389 20 14.00106171123642 29.156406253304173 28.097028448173393 28.74550358728601 21 12.029640410946419 39.12566329607378 25.28272801451978 23.56196827846002 22 17.864968706498825 31.264114524807656 23.282394042166562 27.58852272652696 23 20.91702391256633 28.68508959282353 27.319813529672132 23.078072964938006 24 15.606571817546092 31.777615247806757 26.535926456079356 26.079886478567797 25 14.572003054137667 29.004049223788858 23.812051826547258 32.61189589552622 26 16.130698533814016 34.74199437400177 26.588718160331737 22.53858893185247 27 16.636096715982728 31.567543277150623 23.247733763722643 28.54862624314401 28 15.598406142401542 28.01604314021316 22.79418025379232 33.59137046359298 29 16.017023024799787 31.145574175324963 25.432582644324953 27.404820155550297 30 16.951910794728782 30.67594145714671 30.16553250873915 22.206615239385357 31 19.58219626011142 31.179426334463024 22.876907303448043 26.361470101977513 32 17.36668429623399 30.48416569523495 25.48407477464267 26.665075233888384 33 16.392808402064915 25.273878043473964 29.430526711366916 28.90278684309421 34 15.41442680335042 35.913655873650875 25.360154146252405 23.3117631767463 35 15.118017453184473 30.61758462439372 23.66316372385523 30.601234198566573 36 13.378710394087454 30.826563722327794 27.772084933864218 28.022640949720532 37 12.26713977949563 36.76843842783797 23.22951475685873 27.734907035807677 38 19.683219339532744 28.27911556271157 23.860459872403442 28.177205225352242 39 14.35787961790918 31.68602640666391 29.805247234763254 24.150846740663656 40 14.99576033478847 29.308176911718242 27.58224628102371 28.113816472469583 41 16.46730647973523 33.11006340563957 21.994076424078855 28.428553690546345 42 14.412832610538869 31.982701615029796 26.949415205864575 26.655050568566757 43 15.699410705116188 27.19339142058375 23.91877022389945 33.188427650400605 44 17.51460913925064 28.454715161857507 22.3991046687595 31.63157103013235 45 13.186656204778814 28.46748794171908 34.0915392712694 24.25431658223271 46 13.137908588759178 31.99685828122252 28.784153933423813 26.08107919659449 47 14.980985672031652 32.18238810698118 27.71129793639159 25.12532828459558 48 17.756401472589914 29.236512678097814 23.759306457653906 29.24777939165837 49 15.817961129499986 30.057082808518114 22.14338806057588 31.981568001406018 50 14.648598976983484 34.29531402053081 26.634346139562876 24.42174086292283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 81689.0 1 78367.0 2 46847.5 3 18251.0 4 17269.5 5 12998.0 6 6186.5 7 3269.5 8 3443.5 9 3102.0 10 2491.5 11 2136.0 12 2047.5 13 2010.5 14 2254.0 15 2483.0 16 2473.0 17 2857.0 18 3792.5 19 5171.5 20 6461.5 21 7183.5 22 8101.5 23 9733.0 24 11472.5 25 16311.5 26 22175.0 27 26917.0 28 31311.0 29 39281.5 30 52718.0 31 76245.5 32 115970.5 33 140595.0 34 164463.0 35 201188.5 36 204614.5 37 187087.5 38 179196.5 39 179406.0 40 186846.0 41 200206.5 42 212699.5 43 233321.0 44 245714.5 45 240206.0 46 233532.5 47 213243.0 48 217150.0 49 265060.5 50 315520.5 51 321701.5 52 259437.5 53 169271.0 54 119468.5 55 103596.5 56 85797.0 57 67801.5 58 53095.0 59 46754.0 60 43339.5 61 35012.0 62 23938.0 63 18095.5 64 14180.0 65 9887.5 66 6075.5 67 3266.0 68 1919.5 69 1405.0 70 950.0 71 697.5 72 524.0 73 350.5 74 262.0 75 146.0 76 60.0 77 31.0 78 6.0 79 5.5 80 5.0 81 3.0 82 2.5 83 2.5 84 1.5 85 1.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 13330.0 25 15227.0 26 17092.0 27 19313.0 28 19733.0 29 18203.0 30 22012.0 31 33159.0 32 56723.0 33 8265.0 34 5989.0 35 5674.0 36 6575.0 37 9060.0 38 5439.0 39 5620.0 40 8835.0 41 7523.0 42 11190.0 43 20714.0 44 60008.0 45 150071.0 46 322578.0 47 258404.0 48 549557.0 49 354630.0 50 737818.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.426900398097659 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38411575650785 4.52351701575252 2 11.691292479242808 1.5027754457824354 3 4.75263873993668 0.9163420742914022 4 2.5887680336813057 0.6655101722499551 5 1.5867925722543623 0.5099078907159984 6 1.0774984825705503 0.41549852559493755 7 0.7482654187244839 0.3366319122238168 8 0.5293847571491742 0.2721842485175088 9 0.4236621674897051 0.24505510976086525 >10 3.359395663943643 4.369251753705549 >50 0.5541503941542001 2.5067086449996294 >100 1.497834144808523 26.368476042435308 >500 0.6222885819011256 27.803721078496736 >1k 0.17064696577327376 17.11501124614522 >5k 0.0072359137430363435 3.4787688917804793 >10k+ 0.006029928119196953 8.970639947547644 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 57874 2.110078162656203 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 35998 1.3124821802415247 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 33670 1.2276036171101765 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 19792 0.7216136260720112 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 17375 0.6334901350546278 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 16759 0.6110308588995975 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 15419 0.5621746412896291 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 13498 0.49213524276071174 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 10729 0.3911778796547397 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 10364 0.3778700293356065 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 9459 0.3448738525169338 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 9315 0.3396236321170566 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 9089 0.3313837028783604 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 8543 0.3114766171954927 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 8502 0.3099817627760832 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC 7549 0.27523551249078476 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7084 0.25828167578284794 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7033 0.25642222272455817 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 6603 0.2407444812527026 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT 5899 0.21507673707552516 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 5508 0.200820930295303 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA 5182 0.18893501466780324 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 4940 0.1801117276068985 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 4901 0.17868979291526507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4683 0.1707415425876732 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 4491 0.1637412487211703 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 4446 0.16210055484620867 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG 4380 0.15969420382959826 No Hit TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA 4044 0.14744368956321813 No Hit TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGAC 3909 0.14252160793833324 No Hit ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC 3902 0.142266388891117 No Hit CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA 3777 0.13770890590511248 No Hit CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG 3666 0.13366186101354047 No Hit ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC 3632 0.13242222564134723 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTA 3620 0.1319847072746908 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT 3575 0.13034401339972917 No Hit ACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATA 3366 0.12272390184712963 No Hit AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA 3094 0.1128068188695838 No Hit ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT 3046 0.11105674540295807 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT 3031 0.11050984744463753 No Hit TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC 2984 0.10879623384189982 No Hit CACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG 2976 0.10850455493079553 No Hit ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC 2969 0.10824933588357928 No Hit CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC 2849 0.10387415221701495 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT 2803 0.10219699847816528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09381122978391697 0.0 0.0 0.0 0.0 2 0.15032401881037297 0.0 0.0 0.0 0.0 3 0.19772184186481995 0.0 0.0 0.0 0.0 4 0.2405621819332624 0.0 0.0 0.0 0.0 5 0.27953777642957306 0.0 0.0 0.0 0.0 6 0.3379464783782069 0.0 0.0 0.0 0.0 7 0.38621933816596676 0.0 0.0 0.0 0.0 8 0.4432790251507433 0.0 0.0 0.0 0.0 9 0.49760422234391716 0.0 0.0 0.0 0.0 10 0.5420852562873212 0.0 0.0 0.0 0.0 11 0.5909050140334016 0.0 0.0 0.0 0.0 12 0.6348391500184851 0.0 0.0 0.0 0.0 13 0.6807785785174106 0.0 0.0 0.0 0.0 14 0.7233272396747489 0.0 0.0 0.0 0.0 15 0.7640893675015732 0.0 0.0 0.0 0.0 16 0.8091537592671859 0.0 0.0 0.0 0.0 17 0.8573172394632816 0.0 0.0 0.0 0.0 18 0.8978241482428898 0.0 0.0 0.0 0.0 19 0.9415395250446451 0.0 0.0 0.0 0.0 20 0.9858747195324971 0.0 0.0 0.0 0.0 21 1.02722020518153 0.0 0.0 0.0 0.0 22 1.0600340826807626 0.0 0.0 0.0 0.0 23 1.0945980336466208 0.0 0.0 0.0 0.0 24 1.1387509288150326 0.0 0.0 0.0 0.0 25 1.1751378729752926 0.0 0.0 0.0 0.0 26 1.2186344905937196 0.0 0.0 0.0 0.0 27 1.251229608909624 0.0 0.0 0.0 0.0 28 1.2880540714365405 0.0 0.0 0.0 0.0 29 1.3282328414411564 0.0 0.0 0.0 0.0 30 1.3669532168902507 0.0 0.0 0.0 0.0 31 1.3982722399700738 0.0 0.0 0.0 0.0 32 1.433857067124797 0.0 0.0 0.0 0.0 33 1.4668532439434696 0.0 0.0 0.0 0.0 34 1.5056100792564522 0.0 0.0 0.0 0.0 35 1.5394083730806616 0.0 0.0 0.0 0.0 36 1.5665709716772485 0.0 0.0 0.0 0.0 37 1.588337510418406 0.0 0.0 0.0 0.0 38 1.6046715294402463 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGGA 10 0.006422542 149.87865 44 CGTGCGC 10 0.006422542 149.87865 44 CAAATGC 20 1.8067276E-6 149.87865 44 CGTCGTA 10 0.006422542 149.87865 44 CGAAGGT 70 0.0 149.87865 44 AGGTCGA 10 0.006422542 149.87865 44 TTTAGGC 125 0.0 149.87863 44 GTCGCGC 30 5.16593E-10 149.87863 44 TATATCA 75 0.0 149.87863 44 GGTCATG 120 0.0 149.87863 44 GGCGGTC 105 0.0 149.87863 44 TGTCTAT 210 0.0 146.31009 44 ATTGCAC 120 0.0 143.6337 44 GAGTCTG 110 0.0 143.06598 44 CCACCCG 80 0.0 140.51123 44 GTCATGC 70 0.0 139.17302 44 CCGGGCC 45 0.0 133.22546 44 GGCAAGT 115 0.0 130.32925 44 TGCTCAG 75 0.0 129.89482 44 AAGTCCA 75 0.0 129.89482 44 >>END_MODULE