FastQCFastQC Report
Tue 24 May 2016
ERR841103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131057
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC579541.8509404332147257No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA332201.0609835592261654No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT182510.5829021956483067No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT179900.5745663525129053No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA174250.5565213281010215No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA130720.41749479488875485No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG112280.35860094530377445No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC97180.3103744198844033No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC94910.30312447202334547No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA94350.30133593863030916No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA91250.2914351287760012No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG89740.2866124762340641No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA78130.24953234642486546No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG68190.2177858786984715No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG67920.21692355009825756No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT62970.201114192427669No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT62510.1996450399976749No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC62240.19878271139746098No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA53610.17122013428691973No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA52340.16716399605628388No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT51170.1634272387886902No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC47860.15285572891199362No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA45980.1468513668068004No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC42620.1361201664485827No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41710.13321379968489874No Hit
ACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAAGC41110.1312975139066456No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40720.13005192815078104No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT40470.12925347574317556No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA40120.1281356423725279No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA37170.11871390396278317No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT35930.11475358002106No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG35500.11338024187997855No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC34400.10986705128651442No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA34000.10858952743434565No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT33570.10721618929326422No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC33090.1056831606706617No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT33000.10539571780392373No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG32960.10526796541870684No Hit
AGTGGAGTGAGGGGTAACAAGATGGCGACCGAGACGGTGGAGCTCCATA32880.10501246064827309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCC301.8189894E-11240.7341844
CCTCGTC301.8189894E-11240.7341844
ATAGTTG900.0240.7341544
AGCGCTT600.0220.6729944
TCCGGAC1250.0211.8460744
ACGATTG750.0208.6362944
CCGGGCT1100.0207.9067744
TACGCCC401.4188117E-10180.5506344
GTTAGCG900.0173.8635644
CGATTTC150.0052340482160.4894644
CGGGCAC150.0052340482160.4894644
CGGTCTG150.0052340482160.4894644
CGGGATA950.0152.0426244
TTGGCCC2900.0145.2706344
CTATCAT33500.0134.7392744
AACGAAA1700.0134.5279144
GTAGCGG900.0133.741244
ACTACTT456.0088496E-8133.741244
AATCGCA2000.0132.4037844
TCCGATC1550.0132.015544
Produced by FastQC (version 0.11.5)