##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841103.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3131057
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.44548725877555	39.0	37.0	39.0	35.0	39.0
2	37.934517001766494	39.0	38.0	39.0	35.0	39.0
3	38.15265803209587	39.0	38.0	39.0	35.0	40.0
4	38.78488925624797	39.0	38.0	41.0	35.0	41.0
5	39.30449493573576	40.0	39.0	41.0	35.0	41.0
6	39.36310006493015	41.0	39.0	41.0	36.0	41.0
7	39.35409288301043	41.0	39.0	41.0	35.0	41.0
8	39.19439697201297	40.0	38.0	41.0	35.0	41.0
9	39.21404433071643	40.0	39.0	41.0	35.0	41.0
10	39.23765552655222	40.0	39.0	41.0	35.0	41.0
11	39.166922863429185	40.0	39.0	41.0	35.0	41.0
12	39.06551749137751	40.0	38.0	41.0	35.0	41.0
13	39.002864527857525	40.0	38.0	41.0	35.0	41.0
14	39.036817918038544	40.0	38.0	41.0	35.0	41.0
15	38.981398613950496	40.0	38.0	41.0	35.0	41.0
16	39.06244951784653	40.0	38.0	41.0	35.0	41.0
17	39.09645400898163	40.0	38.0	41.0	35.0	41.0
18	39.059362700838726	40.0	38.0	41.0	35.0	41.0
19	39.09558497338119	41.0	39.0	41.0	35.0	41.0
20	39.07774978226203	40.0	38.0	41.0	35.0	41.0
21	39.121209866188956	40.0	38.0	41.0	35.0	41.0
22	39.10804466351139	40.0	38.0	41.0	35.0	41.0
23	39.09125384814138	40.0	38.0	41.0	35.0	41.0
24	39.00247488308261	40.0	38.0	41.0	35.0	41.0
25	38.971122720425626	40.0	38.0	41.0	35.0	41.0
26	38.929778743846406	40.0	38.0	41.0	35.0	41.0
27	38.91940741974091	40.0	38.0	41.0	35.0	41.0
28	38.89811122583113	40.0	38.0	41.0	35.0	41.0
29	38.80502744307978	40.0	38.0	41.0	35.0	41.0
30	38.756096329034	40.0	38.0	41.0	35.0	41.0
31	38.71361785103072	40.0	38.0	41.0	35.0	41.0
32	38.57708041568353	40.0	37.0	41.0	35.0	41.0
33	38.510429617539614	40.0	37.0	41.0	35.0	41.0
34	38.53247998922265	40.0	37.0	41.0	35.0	41.0
35	38.54188722891965	40.0	37.0	41.0	35.0	41.0
36	38.518746384941004	40.0	37.0	41.0	35.0	41.0
37	38.445854344483415	40.0	37.0	41.0	35.0	41.0
38	38.391513653866376	40.0	37.0	41.0	34.0	41.0
39	38.394651682810725	40.0	37.0	41.0	34.0	41.0
40	38.34364209113821	40.0	36.0	41.0	34.0	41.0
41	38.243830407861296	40.0	36.0	41.0	34.0	41.0
42	38.14428992561469	39.0	36.0	41.0	34.0	41.0
43	37.99664733318049	39.0	36.0	41.0	34.0	41.0
44	37.84296020808418	39.0	35.0	41.0	34.0	41.0
45	37.606750288528524	39.0	35.0	41.0	33.0	41.0
46	37.33936327500775	39.0	35.0	41.0	33.0	41.0
47	37.14587531282074	38.0	35.0	40.0	33.0	41.0
48	37.131140017316596	38.0	35.0	40.0	33.0	41.0
49	37.182147784999664	38.0	35.0	40.0	33.0	41.0
50	37.08202219853555	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	14.0
22	31.0
23	69.0
24	170.0
25	367.0
26	742.0
27	1644.0
28	3391.0
29	7039.0
30	13736.0
31	24393.0
32	41859.0
33	73140.0
34	141492.0
35	117012.0
36	176305.0
37	301200.0
38	422056.0
39	697703.0
40	1108692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.27907253045856	17.62641178362451	38.09451568591693
2	25.778994122432135	20.631243698214373	31.024890316592767	22.56487186276072
3	21.092781127906647	22.61108628811293	28.4575464451781	27.83858613880233
4	19.86581528218745	25.59915070214308	31.07902538982842	23.456008625841047
5	15.908333831035334	28.36942923747476	31.99772473001929	23.72451220147062
6	20.383883142338195	27.4144482198823	27.71463438704565	24.487034250733856
7	23.10159157115313	27.04974709818441	23.04716905505074	26.801492275611714
8	21.05557324571223	24.7492460213915	31.085444947185565	23.109735785710704
9	21.570511172425157	27.984511300816305	28.64240414658692	21.802573380171616
10	24.854450110617595	21.64186087956878	29.879622121219768	23.624066888593852
11	23.90943377907205	22.766720631403388	26.56502261057528	26.758822978949283
12	21.050590902688775	24.54496995742971	28.404241762446357	26.000197377435157
13	20.478036650243034	25.915529484132676	26.982804848330773	26.62362901729352
14	19.00993817742698	25.92412083203851	26.333375598080778	28.73256539245373
15	20.30905218269741	33.70906374428827	26.604753602377727	19.377130470636594
16	18.64791985581866	34.099283404933225	25.51090574205452	21.7418909971936
17	20.97713328118907	34.921210313322305	22.991149634133137	21.11050677135549
18	18.88304812081032	27.736511983014044	24.517822575571124	28.86261732060451
19	22.60869093089011	23.65057550852635	24.313163254453688	29.427570306129848
20	21.60436555450763	22.277397057926446	28.42116895348759	27.697068434078332
21	19.82036737114655	30.940733432831145	25.231319647007382	24.007579549014917
22	21.808833247047243	25.801127223170962	25.590271911370504	26.79976761841129
23	26.22948097080315	24.319678626099748	28.868685558902314	20.58215484419479
24	23.14317497254122	28.208397355908883	24.736790163832854	23.911637507717042
25	21.242684529342007	25.460081407647188	27.15416172558572	26.14307233742508
26	21.83355466451445	33.176493663926024	24.18370595214745	20.806245719412082
27	21.97562032579588	28.63274215350024	24.04295887426401	25.348678646439872
28	23.363292007112282	26.4564534873355	23.54592915921752	26.6343253463347
29	22.26318420598664	28.404753387298797	26.60545522315295	22.726607183561608
30	24.70870880720254	27.84988151561616	28.25871413694991	19.182695540231393
31	23.050193766477108	31.322687275018534	24.44087985683344	21.186239101670918
32	21.24670663165431	26.199615637400186	27.980740729250304	24.572937001695202
33	19.676917368181794	25.339986088366263	33.02968956244078	21.953406981011167
34	21.15765753536231	31.346202686295	23.911139123660092	23.585000654682595
35	19.492024583883087	25.89577299842126	26.869948334118448	27.7422540835772
36	20.45302162683626	26.786206925198265	27.683347311849914	25.07742413611556
37	18.34648073233203	31.31588043064773	25.359701848563937	24.9779369884563
38	24.815862399739544	24.29191080072478	26.795937637218977	24.096289162316705
39	19.129713317080657	30.21768028663387	26.85507204738176	23.797534348903714
40	18.398732217498225	25.274744199434647	28.735313851029932	27.591209732037196
41	18.237415591034704	29.328131971620596	28.093737379437812	24.340715057906888
42	20.031582943652175	29.931691879663248	25.601343780235407	24.43538139644917
43	21.12793367260529	22.962968773454495	27.83979222950026	28.069305324439952
44	19.85971196556166	26.306729411221603	23.697057478494074	30.13650114472266
45	19.354922439619667	24.61193477374398	32.048785772647044	23.984357013989307
46	18.563171433396835	28.65054984453473	29.5985290450121	23.18774967705633
47	20.195861818118498	31.427323531374938	27.39443902184207	20.98237562866449
48	26.172415629455926	26.090918719098337	24.30199571692449	23.434669934521242
49	17.049002861642894	29.105170922158873	25.460458306528537	28.38536790966969
50	18.267834522816447	31.248648314641457	29.071662495751845	21.41185466679025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17820.0
1	18460.0
2	28082.5
3	36451.0
4	33601.5
5	21928.0
6	9614.0
7	7667.0
8	9263.5
9	10081.5
10	10572.5
11	10747.5
12	9890.5
13	8327.0
14	8103.5
15	8858.0
16	8817.5
17	8934.5
18	9440.5
19	9459.0
20	9382.5
21	9646.5
22	10260.5
23	13487.0
24	16720.5
25	17162.0
26	20208.5
27	31149.0
28	40794.0
29	40951.5
30	44646.5
31	64623.0
32	98298.0
33	115522.0
34	127344.5
35	146806.0
36	141181.0
37	128994.5
38	129824.0
39	138126.0
40	156036.0
41	179263.5
42	198736.0
43	219763.0
44	239880.5
45	240331.0
46	263847.5
47	303286.0
48	324189.0
49	341598.0
50	374593.0
51	395071.5
52	348206.0
53	281275.5
54	231443.0
55	194359.5
56	170409.5
57	150348.0
58	135168.0
59	119024.0
60	91156.0
61	63767.0
62	47564.0
63	34704.5
64	27057.0
65	23647.0
66	14701.5
67	6642.0
68	4420.5
69	3432.5
70	2647.0
71	1815.5
72	1252.0
73	871.5
74	620.0
75	493.5
76	281.5
77	155.0
78	93.5
79	48.5
80	28.5
81	12.5
82	8.5
83	6.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10957.0
25	10130.0
26	10913.0
27	14252.0
28	18276.0
29	17604.0
30	30343.0
31	53153.0
32	44133.0
33	11489.0
34	10021.0
35	10829.0
36	13103.0
37	13185.0
38	8575.0
39	8811.0
40	10128.0
41	11479.0
42	19043.0
43	31909.0
44	114408.0
45	248453.0
46	342385.0
47	396249.0
48	544254.0
49	609103.0
50	517872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.547942384095953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69179904494493	4.628858271743887
2	11.327322464129535	1.48341309722392
3	4.5667790456082695	0.8970901821461895
4	2.5144367964206022	0.6585754908565162
5	1.5410550676515582	0.5045369896850748
6	1.0147291062094308	0.3986632633754721
7	0.7294428644473633	0.3343444894223874
8	0.5424311828781013	0.2841446506258258
9	0.42637544036134073	0.25126936357316393
>10	3.538309733881309	4.88397251376455
>50	0.6679332955348242	3.1181135006441245
>100	1.6425441353222936	28.578151976706636
>500	0.6128352960585394	28.103728214953772
>1k	0.173090885146305	16.624538337442768
>5k	0.007277094270415226	3.4937996676729317
>10k+	0.003638547135207613	5.756799990162782
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	57954	1.8509404332147257	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	33220	1.0609835592261654	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	18251	0.5829021956483067	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	17990	0.5745663525129053	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	17425	0.5565213281010215	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	13072	0.41749479488875485	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	11228	0.35860094530377445	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	9718	0.3103744198844033	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	9491	0.30312447202334547	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9435	0.30133593863030916	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	9125	0.2914351287760012	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	8974	0.2866124762340641	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	7813	0.24953234642486546	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	6819	0.2177858786984715	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	6792	0.21692355009825756	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6297	0.201114192427669	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	6251	0.1996450399976749	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC	6224	0.19878271139746098	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5361	0.17122013428691973	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	5234	0.16716399605628388	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	5117	0.1634272387886902	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	4786	0.15285572891199362	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	4598	0.1468513668068004	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	4262	0.1361201664485827	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4171	0.13321379968489874	No Hit
ACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAAGC	4111	0.1312975139066456	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4072	0.13005192815078104	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4047	0.12925347574317556	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	4012	0.1281356423725279	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	3717	0.11871390396278317	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	3593	0.11475358002106	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	3550	0.11338024187997855	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	3440	0.10986705128651442	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	3400	0.10858952743434565	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	3357	0.10721618929326422	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	3309	0.1056831606706617	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	3300	0.10539571780392373	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	3296	0.10526796541870684	No Hit
AGTGGAGTGAGGGGTAACAAGATGGCGACCGAGACGGTGGAGCTCCATA	3288	0.10501246064827309	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04717256824133192	0.0	0.0	0.0	0.0
2	0.06726163081668586	0.0	0.0	0.0	0.0
3	0.08466789330248539	0.0	0.0	0.0	0.0
4	0.12261035171189794	0.0	0.0	0.0	0.0
5	0.1521530907933008	0.0	0.0	0.0	0.0
6	0.2290919647901651	0.0	0.0	0.0	0.0
7	0.2571336133452697	0.0	0.0	0.0	0.0
8	0.2975991813627155	0.0	0.0	0.0	0.0
9	0.3390867684618964	0.0	0.0	0.0	0.0
10	0.3686933837359077	0.0	0.0	0.0	0.0
11	0.4014299324477325	0.0	0.0	0.0	0.0
12	0.44055410042040116	0.0	0.0	0.0	0.0
13	0.47597344922178036	0.0	0.0	0.0	0.0
14	0.5075921645629575	0.0	0.0	0.0	0.0
15	0.5427879466902071	0.0	0.0	0.0	0.0
16	0.5804110241365775	0.0	0.0	0.0	0.0
17	0.6278710352446474	0.0	0.0	0.0	0.0
18	0.6600965744156047	0.0	0.0	0.0	0.0
19	0.6925456802606915	0.0	0.0	0.0	0.0
20	0.7291467386253269	0.0	0.0	0.0	0.0
21	0.7590727348623804	0.0	0.0	0.0	0.0
22	0.7839205737870629	0.0	0.0	0.0	0.0
23	0.8106208222973903	0.0	0.0	0.0	0.0
24	0.8400358089935763	0.0	0.0	0.0	0.0
25	0.8687481575710695	0.0	0.0	0.0	0.0
26	0.9004626872011592	0.0	0.0	0.0	0.0
27	0.9280891405043089	0.0	0.0	0.0	0.0
28	0.9594842891713565	0.0	0.0	0.0	0.0
29	0.990719747356883	0.0	0.0	0.0	0.0
30	1.0182823244674242	0.0	0.0	0.0	0.0
31	1.0474098682968722	0.0	0.0	0.0	0.0
32	1.075611207333498	0.0	0.0	0.0	0.0
33	1.1024392082290422	0.0	0.0	0.0	0.0
34	1.1324929568513125	0.0	0.0	0.0	0.0
35	1.1590335148801187	0.0	0.0	0.0	0.0
36	1.1821886347006778	0.0	0.0	0.0	0.0
37	1.1984131876232211	0.0	0.0	0.0	0.0
38	1.2116674975894721	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCC	30	1.8189894E-11	240.73418	44
CCTCGTC	30	1.8189894E-11	240.73418	44
ATAGTTG	90	0.0	240.73415	44
AGCGCTT	60	0.0	220.67299	44
TCCGGAC	125	0.0	211.84607	44
ACGATTG	75	0.0	208.63629	44
CCGGGCT	110	0.0	207.90677	44
TACGCCC	40	1.4188117E-10	180.55063	44
GTTAGCG	90	0.0	173.86356	44
CGATTTC	15	0.0052340482	160.48946	44
CGGGCAC	15	0.0052340482	160.48946	44
CGGTCTG	15	0.0052340482	160.48946	44
CGGGATA	95	0.0	152.04262	44
TTGGCCC	290	0.0	145.27063	44
CTATCAT	3350	0.0	134.73927	44
AACGAAA	170	0.0	134.52791	44
GTAGCGG	90	0.0	133.7412	44
ACTACTT	45	6.0088496E-8	133.7412	44
AATCGCA	200	0.0	132.40378	44
TCCGATC	155	0.0	132.0155	44
>>END_MODULE