FastQCFastQC Report
Tue 24 May 2016
ERR841102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14340105
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA677460.47242331907611557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA646880.4510985100876179No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA624040.4351711511177917No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC574470.40060376126953046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA558940.389773993984005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA483930.33746614826042065No Hit
AAAAAAAAAAAAAAAAAAAAAAAA452730.3157089853944584No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA410000.2859114350975812No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA393190.27418906625858036No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC348760.24320602952349374No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA318710.222250813365732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316020.22037495541350638No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG242490.169099180236128No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG232530.16215362439814773No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA214970.1499082468364074No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT212320.14806028268272792No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT212250.14801146853527222No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT208830.1456265487595802No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA197120.13746063923520785No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA195800.13654014388318633No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT193370.13484559562151044No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT189600.13221660510854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179770.12536170411583455No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC144650.10087094899235396No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA144210.10056411720834679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG1050.0129.3106544
CGCGTAT1150.0110.6871144
CCGCGTA750.0105.2555443
GAATACA122700.0103.1185344
GCCACGC1450.097.9965443
CCCGAGC47250.097.3421844
ATGCGGG1900.093.7927644
CTATCAT206000.092.4810544
CGTACGT307.4345917E-787.7129543
GCAACCC2550.086.5240444
CGACACC1950.083.6646543
AACGACA30650.082.9338143
CCAGAAT75050.082.42908544
GTAGCGT556.184564E-1176.5494843
ACTTCTA155350.074.399444
TTCGCGT1750.072.7372444
CTCGTCG550.071.15114642
AATCGCA16550.070.759544
CTATACG5000.067.8880944
TACGTTT2000.067.8880944
Produced by FastQC (version 0.11.5)