##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841102.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14340105
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.68103232159039	39.0	38.0	39.0	35.0	39.0
2	38.03727504087313	39.0	38.0	39.0	35.0	39.0
3	38.179027210749155	39.0	38.0	39.0	35.0	39.0
4	38.55428701533217	39.0	38.0	40.0	35.0	41.0
5	39.40364606814246	40.0	39.0	41.0	36.0	41.0
6	39.3691706580949	40.0	39.0	41.0	36.0	41.0
7	39.37438700762651	40.0	39.0	41.0	36.0	41.0
8	39.336933725380675	40.0	39.0	41.0	36.0	41.0
9	39.278110934334165	40.0	39.0	41.0	36.0	41.0
10	39.2705780048333	40.0	39.0	41.0	36.0	41.0
11	39.1729368787746	40.0	39.0	41.0	35.0	41.0
12	39.070600668544614	40.0	38.0	41.0	35.0	41.0
13	39.009050142938285	40.0	38.0	41.0	35.0	41.0
14	38.97698447814712	40.0	38.0	41.0	35.0	41.0
15	38.86774790003281	40.0	38.0	41.0	35.0	41.0
16	38.86080541251267	40.0	38.0	41.0	35.0	41.0
17	38.9511074709704	40.0	38.0	41.0	35.0	41.0
18	38.951869250608695	40.0	38.0	41.0	35.0	41.0
19	38.917939861667676	40.0	38.0	41.0	35.0	41.0
20	38.91733163739038	40.0	38.0	41.0	35.0	41.0
21	38.92318201296295	40.0	38.0	41.0	35.0	41.0
22	38.9634398771836	40.0	38.0	41.0	35.0	41.0
23	38.91337197321777	40.0	38.0	41.0	35.0	41.0
24	38.80149859432689	40.0	38.0	41.0	35.0	41.0
25	38.79926494110247	40.0	38.0	41.0	35.0	41.0
26	38.80462414109601	40.0	38.0	41.0	35.0	41.0
27	38.736175563052086	40.0	38.0	41.0	35.0	41.0
28	38.66920084259557	40.0	38.0	41.0	35.0	41.0
29	38.662805868081286	40.0	38.0	41.0	35.0	41.0
30	38.680681798365555	40.0	38.0	41.0	35.0	41.0
31	38.58608056318602	40.0	37.0	41.0	35.0	41.0
32	38.50019268875176	40.0	37.0	41.0	35.0	41.0
33	38.28467332056	40.0	37.0	41.0	34.0	41.0
34	38.311419857491074	40.0	37.0	41.0	34.0	41.0
35	38.34688064508133	40.0	37.0	41.0	34.0	41.0
36	38.319887543965784	40.0	37.0	41.0	34.0	41.0
37	38.237904890708336	40.0	36.0	41.0	34.0	41.0
38	38.143078488462656	40.0	36.0	41.0	34.0	41.0
39	38.094236674252635	40.0	36.0	41.0	34.0	41.0
40	37.997311114770255	39.0	36.0	41.0	34.0	41.0
41	37.88205034451437	39.0	35.0	41.0	34.0	41.0
42	37.78042676432768	39.0	35.0	41.0	34.0	41.0
43	37.69712457144336	39.0	35.0	41.0	34.0	41.0
44	37.53110684313732	39.0	35.0	41.0	34.0	41.0
45	37.372854815958455	39.0	35.0	41.0	33.0	41.0
46	37.10468430565666	38.0	35.0	40.0	33.0	41.0
47	36.90138176622569	38.0	35.0	40.0	32.0	41.0
48	36.75691238047805	38.0	35.0	40.0	32.0	41.0
49	36.96580446452424	38.0	35.0	40.0	33.0	41.0
50	36.55199865080064	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	13.0
20	39.0
21	98.0
22	228.0
23	518.0
24	1134.0
25	2288.0
26	4579.0
27	9338.0
28	18780.0
29	37429.0
30	69239.0
31	121532.0
32	201555.0
33	339065.0
34	585062.0
35	631385.0
36	1025164.0
37	1579304.0
38	2193816.0
39	3022496.0
40	4497039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.866167995283156	17.293904054398485	29.839927950318355
2	24.43817531322121	27.11409714224547	29.16006542490449	19.28766211962883
3	26.226516472508393	23.220464564241336	24.307611415676526	26.245407547573745
4	21.12390390446932	26.74484601054176	28.43395498150118	23.697295103487736
5	17.651872144590293	27.306194759382862	28.399220228861644	26.642712867165198
6	21.162822726890774	29.162589813672913	27.952828797278684	21.721758662157633
7	23.178930698206184	27.85818513881175	25.19574298793489	23.767141175047186
8	20.91176459307655	26.518975976814673	25.992166724023292	26.577092706085487
9	21.62556689787139	29.186334409685283	27.102528189298475	22.08557050314485
10	24.088526548445774	24.592002638753343	26.96338694870086	24.35608386410002
11	22.11936384008346	26.946420545735194	26.947731554266863	23.986484059914485
12	20.498183242033445	26.650104723779915	28.782941268561146	24.068770765625498
13	22.516132204052898	28.08123092543604	24.43604143763243	24.96659543287863
14	19.338045293252733	28.6043512233697	24.62908744392039	27.428516039457172
15	20.040215884053847	29.737055621280316	28.539672477990923	21.68305601667491
16	21.428783122578253	32.32341046317303	23.153491553932138	23.094314860316572
17	21.753717981841834	34.812869222366224	21.1152707738193	22.31814202197264
18	20.496997755595235	30.6250198307474	23.58700999748607	25.290972416171293
19	24.440385896755988	28.620229768192075	23.096783461487906	23.842600873564034
20	24.12868664490253	26.26012152630682	27.614776879248794	21.99641494954186
21	20.298575219637513	31.39668782062614	24.49550404268309	23.809232917053258
22	23.158554278368253	29.27385120262369	22.912370585849963	24.65522393315809
23	24.95814361191916	28.784468454031543	23.952181661152412	22.305206272896886
24	20.330395070328983	32.494748120742486	24.946184145792515	22.22867266313601
25	21.302592547911658	28.700010630523543	26.55088834430193	23.44650847726287
26	22.872163878724443	31.123369806397385	26.483036867558795	19.521429447319374
27	24.252210482262043	29.080222981873703	24.168551214162797	22.499015321701453
28	24.85005997456683	27.892514433624093	24.534754180773252	22.722671411035826
29	24.220877236625647	30.469320624743695	23.086967299609213	22.222834839021445
30	25.643917122239568	29.85292290064951	23.97985308109633	20.523306896014596
31	22.455050166158983	27.300566433161617	26.034280345053528	24.21010305562587
32	21.597466161827686	28.95188213107253	27.294804257884277	22.155847449215507
33	20.55957086566647	26.39105732831067	30.024971445728312	23.02440036029455
34	20.55881698043109	29.561349274286563	26.650578720123335	23.22925502515901
35	20.250196318457924	27.687762215903376	26.38962833645889	25.672413129179805
36	21.603474679323366	28.05000072745056	26.95882489044829	23.38769970277778
37	19.23455144887415	30.864202574030557	24.675339570550797	25.22590640654449
38	20.346519567120968	28.663140996597207	25.733540692200968	25.256798744080854
39	22.231710190130414	29.162471116342935	26.405528528287935	22.200290165238712
40	18.417008596470385	29.03001550747075	28.47050124897405	24.08247464708481
41	20.416457835547522	29.200768418544826	25.816556666948976	24.566217078958676
42	20.41885881384018	31.377182144051545	24.684348087265107	23.519610954843166
43	20.81858311360476	27.442561582967635	25.527892421214116	26.21096288221349
44	19.472692536987875	31.202533769070474	23.5144013094645	25.810372384477155
45	18.5385534107802	28.343433575738032	27.670564231976062	25.447448781505706
46	18.449105518008547	30.985297288930465	24.71717415233999	25.848423040721
47	21.12614495211595	27.944261738109354	29.531709545184793	21.3978837645899
48	20.520546383912	31.420491678682406	24.615807055515646	23.44315488188995
49	20.320241941554407	30.293430885333105	23.72483280911679	25.661494363995697
50	19.321818374483417	30.35080675853361	26.82587418118971	23.501500685793257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503970.0
1	502328.5
2	325333.5
3	148725.0
4	143387.0
5	104668.0
6	45825.5
7	23284.5
8	25329.5
9	23954.0
10	20555.5
11	18144.5
12	16630.0
13	16304.0
14	19830.5
15	24452.0
16	26407.0
17	27403.0
18	29976.5
19	36572.5
20	46818.0
21	58470.5
22	68485.5
23	81684.5
24	96167.5
25	111863.0
26	126738.5
27	138425.5
28	154685.5
29	184666.0
30	257351.0
31	413835.0
32	585619.5
33	601939.0
34	530453.0
35	516927.5
36	527667.5
37	565221.5
38	621495.0
39	683469.5
40	753480.5
41	833181.0
42	933775.0
43	1045184.0
44	1104670.5
45	1099573.5
46	1120814.0
47	1150102.5
48	1207064.5
49	1328472.5
50	1483113.0
51	1530214.0
52	1408853.5
53	1205442.5
54	1046932.0
55	944771.0
56	828326.0
57	715120.5
58	625877.5
59	563380.5
60	490002.5
61	390401.0
62	293343.5
63	225510.5
64	192138.0
65	170028.5
66	114810.0
67	64801.5
68	41606.0
69	29321.0
70	21081.5
71	15722.0
72	12266.5
73	8887.5
74	6177.0
75	4315.0
76	2996.5
77	2288.5
78	1383.5
79	651.0
80	549.5
81	709.5
82	820.5
83	702.5
84	419.0
85	164.0
86	64.5
87	44.0
88	41.0
89	41.0
90	23.5
91	3.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	107504.0
25	108205.0
26	122361.0
27	145328.0
28	145036.0
29	120908.0
30	151225.0
31	221330.0
32	295448.0
33	51426.0
34	42688.0
35	44272.0
36	54766.0
37	82934.0
38	40064.0
39	47502.0
40	54985.0
41	62174.0
42	76314.0
43	115742.0
44	355459.0
45	885716.0
46	1607779.0
47	1381596.0
48	2599191.0
49	2070017.0
50	3350135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.081831954417378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.67142768016298	6.92333793941329
2	12.681995920658876	2.55715503437379
3	5.373136953330814	1.625131914946543
4	2.831641606986263	1.1419253934708753
5	1.7207240625496223	0.8674025419273834
6	1.1008953329543814	0.6659425047749064
7	0.8012344570990133	0.5654537806792764
8	0.6166454225554382	0.49735324205316983
9	0.49550330674434234	0.44960229643091276
>10	3.8868544302679893	7.587176530257116
>50	0.4464192128238619	3.1934513885172064
>100	1.0862789095999592	29.790960393282763
>500	0.21756430292958853	13.98795236833866
>1k	0.05767983845110022	11.088784177667781
>5k	0.006216122940845387	4.2470375696718135
>10k+	0.005782439944972452	14.81133292419448
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	67746	0.47242331907611557	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	64688	0.4510985100876179	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	62404	0.4351711511177917	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	57447	0.40060376126953046	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	55894	0.389773993984005	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48393	0.33746614826042065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	45273	0.3157089853944584	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA	41000	0.2859114350975812	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	39319	0.27418906625858036	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	34876	0.24320602952349374	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	31871	0.222250813365732	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31602	0.22037495541350638	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	24249	0.169099180236128	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	23253	0.16215362439814773	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA	21497	0.1499082468364074	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	21232	0.14806028268272792	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	21225	0.14801146853527222	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	20883	0.1456265487595802	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	19712	0.13746063923520785	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	19580	0.13654014388318633	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT	19337	0.13484559562151044	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	18960	0.13221660510854	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17977	0.12536170411583455	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	14465	0.10087094899235396	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	14421	0.10056411720834679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0839045460266853	0.0	0.0	0.0	0.0
2	0.13847178943250416	0.0	0.0	0.0	0.0
3	0.18457326497958	0.0	0.0	0.0	0.0
4	0.2437290382462332	0.0	0.0	0.0	0.0
5	0.29065338085041914	0.0	0.0	0.0	0.0
6	0.37701258114916175	0.0	0.0	0.0	0.0
7	0.4323190102164524	0.0	0.0	0.0	0.0
8	0.49997541859003125	0.0	0.0	0.0	0.0
9	0.5642706242388044	0.0	0.0	0.0	0.0
10	0.6181474961306072	0.0	0.0	0.0	0.0
11	0.6855807541158171	0.0	0.0	0.0	0.0
12	0.7419401740782233	0.0	0.0	0.0	0.0
13	0.8008449031579615	0.0	0.0	0.0	0.0
14	0.8545683591577607	0.0	0.0	0.0	0.0
15	0.9179988570516046	0.0	0.0	0.0	0.0
16	0.9729426667377958	0.0	0.0	0.0	0.0
17	1.0369868281996542	0.0	0.0	0.0	0.0
18	1.084999028947138	0.0	0.0	0.0	0.0
19	1.1318117963571397	0.0	0.0	0.0	0.0
20	1.1849076418896514	0.0	0.0	0.0	0.0
21	1.2309951705374542	0.0	0.0	0.0	0.0
22	1.2709251431562043	0.0	0.0	0.0	0.0
23	1.3112316820553267	0.0	0.0	0.0	0.0
24	1.3526888401444759	0.0	0.0	0.0	0.0
25	1.3919144943499369	0.0	0.0	0.0	0.0
26	1.4412865177765435	0.0	0.0	0.0	0.0
27	1.477130048908289	0.0	0.0	0.0	0.0
28	1.517882888584149	0.0	0.0	0.0	0.0
29	1.5558672687543083	0.0	0.0	0.0	0.0
30	1.589291012862179	0.0	0.0	0.0	0.0
31	1.6206645627769114	0.0	0.0	0.0	0.0
32	1.654311457273151	0.0	0.0	0.0	0.0
33	1.686319591104807	0.0	0.0	0.0	0.0
34	1.7191366450943002	0.0	0.0	0.0	0.0
35	1.7491224785313637	0.0	0.0	0.0	0.0
36	1.774749905945598	0.0	0.0	0.0	0.0
37	1.7918906451521799	0.0	0.0	0.0	0.0
38	1.808306145596563	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	105	0.0	129.31065	44
CGCGTAT	115	0.0	110.68711	44
CCGCGTA	75	0.0	105.25554	43
GAATACA	12270	0.0	103.11853	44
GCCACGC	145	0.0	97.99654	43
CCCGAGC	4725	0.0	97.34218	44
ATGCGGG	190	0.0	93.79276	44
CTATCAT	20600	0.0	92.48105	44
CGTACGT	30	7.4345917E-7	87.71295	43
GCAACCC	255	0.0	86.52404	44
CGACACC	195	0.0	83.66465	43
AACGACA	3065	0.0	82.93381	43
CCAGAAT	7505	0.0	82.429085	44
GTAGCGT	55	6.184564E-11	76.54948	43
ACTTCTA	15535	0.0	74.3994	44
TTCGCGT	175	0.0	72.73724	44
CTCGTCG	55	0.0	71.151146	42
AATCGCA	1655	0.0	70.7595	44
CTATACG	500	0.0	67.88809	44
TACGTTT	200	0.0	67.88809	44
>>END_MODULE