FastQCFastQC Report
Tue 24 May 2016
ERR841101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246214
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA326230.3183907734115255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA238160.23243707383039236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA228040.22256025493904383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA218560.2133080570052509No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC213500.20836964755957663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA185220.1807692089975868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA179900.17557704728790557No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT169840.16575878661132787No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA150870.14724463104128024No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC143730.1402762034835501No Hit
AAAAAAAAAAAAAAAAAAAAAAAA142620.13919287651028955No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG140280.13690910613422674No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC121630.11870726104295695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121090.11818023711001938No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA117830.11499857410747032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT900.0148.1183244
TTACGTA151.1302576E-4148.1183244
CACGCGC850.0130.6926344
TGCGCCC750.0128.369244
CGGATCT850.0104.5541144
GTCCGTA800.092.57394444
CGCTTTA2050.087.4190643
GCCCGCA503.274181E-1181.9241543
AACGACA23450.081.4437843
CACGTTA553.794048E-880.7918144
CCCGTCG900.079.64847643
CGTTTAG950.077.9570144
TTAATCG1950.076.14744643
TGACACG1950.075.95811544
TCGCAAA4650.069.37125443
TCGGATA900.068.5121942
ACGAGCG1200.067.88756644
CGTTATT709.094947E-1265.831943
GATCTAC1850.064.0511644
GTCGCAA1200.064.0032443
Produced by FastQC (version 0.11.5)