FastQCFastQC Report
Tue 24 May 2016
ERR841099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9470665
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA776150.8195306243014615No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC482720.5097002164050782No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG432200.4563565494080933No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT406880.42962136238585147No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC319260.33710409987049483No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA264460.2792412148460536No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC264310.27908283103668013No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA183270.1935133382925064No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT170990.1805469837651316No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG142760.15073915084104442No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA124590.13155359206560469No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT123200.13008590209874385No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG104210.11003451183206248No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102710.10845067373832777No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA101040.1066873339939698No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT100980.1066239804702204No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG99560.10512461374148488No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA97230.10266438523588366No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT96230.10160849317339385No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT95080.10039421730153057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA1250.0154.6123844
CATAGCG1450.0151.4619644
CTATGCG650.0148.6657444
CCGTCAA1600.0148.243444
CGTTAAT303.5041012E-8146.4132444
CGAATAT600.0146.4132444
ATACCCG1650.0133.1029444
ACGCAAT1850.0128.2105144
CATACGT700.0125.4970644
AATCGCA5800.0122.6841944
CTATCAT143400.0118.355844
GCGGATT2150.0102.1487744
AACCGAG1250.098.38969444
CGTCACG650.097.5790943
CGATTAC1450.096.9356544
ACTTCTA111450.095.7696344
GTACGCT459.094947E-1293.9650443
AACGACA15800.089.6533543
GCCGTCA1600.089.1933843
ACCACCG2950.086.3589944
Produced by FastQC (version 0.11.5)