##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841099.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9470665
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.2630275698697	39.0	37.0	39.0	34.0	39.0
2	37.80664113871624	39.0	38.0	39.0	35.0	39.0
3	37.90685997234619	39.0	38.0	39.0	35.0	39.0
4	38.207037309418084	39.0	38.0	40.0	35.0	41.0
5	38.91531069887912	40.0	38.0	41.0	35.0	41.0
6	38.93245300092443	40.0	38.0	41.0	35.0	41.0
7	38.93242692039049	40.0	38.0	41.0	35.0	41.0
8	38.81842890652346	40.0	38.0	41.0	35.0	41.0
9	38.76525608286219	40.0	38.0	41.0	35.0	41.0
10	38.753919286554854	40.0	38.0	41.0	35.0	41.0
11	38.630955165239186	40.0	38.0	41.0	35.0	41.0
12	38.556834287771764	40.0	38.0	41.0	35.0	41.0
13	38.51281900479005	40.0	38.0	41.0	34.0	41.0
14	38.48188115618069	40.0	38.0	41.0	34.0	41.0
15	38.44211942878351	40.0	38.0	41.0	34.0	41.0
16	38.507336813201604	40.0	38.0	41.0	35.0	41.0
17	38.687919274940036	40.0	38.0	41.0	35.0	41.0
18	38.704513674594125	40.0	38.0	41.0	35.0	41.0
19	38.7406898037255	40.0	38.0	41.0	35.0	41.0
20	38.73610871042319	40.0	38.0	41.0	35.0	41.0
21	38.804775166263404	40.0	38.0	41.0	35.0	41.0
22	38.821321945185474	40.0	38.0	41.0	35.0	41.0
23	38.76344902918644	40.0	38.0	41.0	35.0	41.0
24	38.64834475720554	40.0	38.0	41.0	35.0	41.0
25	38.64853085907996	40.0	38.0	41.0	35.0	41.0
26	38.57397279084508	40.0	37.0	41.0	35.0	41.0
27	38.467505749691895	40.0	37.0	41.0	34.0	41.0
28	38.45611177864264	40.0	37.0	41.0	34.0	41.0
29	38.391351856539934	40.0	37.0	41.0	34.0	41.0
30	38.37993234563301	40.0	37.0	41.0	34.0	41.0
31	38.2507217002017	40.0	37.0	41.0	34.0	41.0
32	38.109455897027075	40.0	36.0	41.0	34.0	41.0
33	37.95189405492081	40.0	36.0	41.0	34.0	41.0
34	37.95699043947412	40.0	36.0	41.0	34.0	41.0
35	37.966806141618086	39.0	36.0	41.0	34.0	41.0
36	37.98191877964913	39.0	36.0	41.0	34.0	41.0
37	37.90275865147936	39.0	35.0	41.0	34.0	41.0
38	37.835538769867014	39.0	35.0	41.0	34.0	41.0
39	37.82161989669323	39.0	35.0	41.0	34.0	41.0
40	37.75048380873611	39.0	35.0	41.0	34.0	41.0
41	37.62955104361087	39.0	35.0	41.0	34.0	41.0
42	37.53489578126635	39.0	35.0	41.0	34.0	41.0
43	37.420732758285304	39.0	35.0	40.0	34.0	41.0
44	37.218401311629385	38.0	35.0	40.0	33.0	41.0
45	37.09657116188072	38.0	35.0	40.0	33.0	41.0
46	36.745267338493456	37.0	35.0	40.0	32.0	41.0
47	36.51673370747261	37.0	35.0	40.0	32.0	41.0
48	36.40412726108642	37.0	35.0	40.0	32.0	41.0
49	36.64340873186745	37.0	35.0	40.0	32.0	41.0
50	36.16836170825592	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	17.0
22	44.0
23	149.0
24	377.0
25	1059.0
26	2349.0
27	5640.0
28	12505.0
29	26286.0
30	51435.0
31	93318.0
32	159316.0
33	272095.0
34	483576.0
35	489797.0
36	780502.0
37	1174430.0
38	1493880.0
39	2203204.0
40	2220680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.150466730688926	16.387867166666755	31.461666102644326
2	24.12467339938642	24.181628217237122	30.521721547536522	21.171976835839935
3	24.564990948365296	21.918840968400847	25.315709086954296	28.200458996279565
4	22.040141848539673	23.99582289100079	29.455450066072448	24.508585194387088
5	16.389239826347993	26.28916765612552	29.598407292412944	27.72318522511355
6	21.0510138411611	27.664181976661617	27.26412559202548	24.0206785901518
7	23.3373897186734	25.85143704269975	24.871611444391707	25.939561794235143
8	20.89724428010071	24.43107215807971	29.38688043553436	25.284803126285222
9	19.421191648104962	28.763048846094758	27.728380214060998	24.087379291739282
10	25.49063872494698	21.459232271440285	30.514625952876596	22.535503050736143
11	20.588057966362445	24.144661436129354	27.99911093888338	27.26816965862482
12	20.899767862130062	26.74391924959863	28.01414684185324	24.342166046418072
13	24.076070687750015	24.83367324258645	26.639660467348385	24.450595602315147
14	16.743027020805826	28.318307109374054	25.34692125632149	29.591744613498626
15	19.32415516756215	29.655055901565518	29.291079348704656	21.729709582167672
16	21.229881956546873	32.93376969832636	23.71648664586911	22.119861699257655
17	21.594270307312105	36.11912151892185	21.86351222432638	20.423095949439666
18	20.648866790241236	29.180738628174474	24.73191692452431	25.438477657059984
19	23.541071297527683	24.132402529283848	27.064224106754914	25.262302066433563
20	22.967003900993223	22.986537904149287	29.0431347745908	25.00332342026669
21	19.913680823891458	31.42442478960031	25.132237282176067	23.529657104332166
22	24.45355210009012	25.988671334061547	23.632004721949304	25.92577184389903
23	26.8374184917321	25.465413463574098	28.311887285634114	19.385280759059686
24	20.042731951768964	28.323333155591502	26.656586417110095	24.97734847552944
25	21.57030912908621	25.740811617496096	28.685790251333216	24.003089002084483
26	22.667897559032454	31.413008035377597	26.085685753434092	19.833408652155853
27	22.46625658400639	26.294671083072718	27.213928634612	24.02514369830889
28	21.478791430238335	25.24271189172529	26.49813079264594	26.780365885390434
29	22.23039134064825	29.840420905744004	25.86594002466039	22.063247728947356
30	23.18605925768667	29.165043727294194	26.90325837122599	20.74563864379315
31	24.327227606412926	28.654717589631744	23.999027019344823	23.019027784610508
32	20.504189334109828	30.20579582256186	26.303807065140195	22.98620777818812
33	19.340732225324267	24.219703185222887	31.735797379027346	24.703767210425497
34	20.482182252725305	29.506770478738876	26.255333394726023	23.7557138738098
35	18.149943186035756	26.155424734344756	28.787778431249393	26.906853648370095
36	21.065881734101993	26.0822952974525	28.079931102570875	24.77189186587463
37	18.04685155558174	32.49793343925416	23.603172930171418	25.85204207499268
38	21.78144099837551	26.53636409408499	25.56267712698511	26.119517780554386
39	19.263809534975586	30.26897440918763	28.473950486253642	21.993265569583137
40	17.594478048903163	26.19296604618531	30.853934352900144	25.35862155201139
41	18.09744104935976	30.318169445218736	26.024637664066102	25.559751841355403
42	18.390214843427252	32.379877410131094	25.50154529427393	23.72836245216772
43	18.67357768040908	25.33588153976053	27.573834285157833	28.416706494672557
44	18.643183526201895	27.000935090198357	24.66813237635086	29.687749007248886
45	17.624404472377204	26.510484819408585	30.601616011347755	25.263494696866452
46	19.056707016153325	30.110323728788213	27.40662861025002	23.426340644808437
47	21.415356086579724	29.564111418173944	26.950059775813713	22.070472719432622
48	21.491639550129545	29.755913089138076	24.619506335694773	24.132941025037606
49	17.076888782431592	30.456337201333056	24.718775051886393	27.74799896434896
50	17.234027085697143	30.251753245378893	28.93653164307688	23.577688025847085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33598.0
1	38431.0
2	67026.5
3	89514.5
4	82529.0
5	51009.5
6	18370.0
7	13634.5
8	17867.5
9	19783.0
10	18603.5
11	15529.0
12	12180.0
13	10425.5
14	10470.5
15	10988.0
16	11556.0
17	12886.0
18	17487.5
19	25941.5
20	31998.5
21	36110.0
22	41111.5
23	50885.0
24	64682.5
25	79589.0
26	92992.0
27	110360.5
28	134230.5
29	159226.5
30	196831.5
31	276482.0
32	384886.5
33	420659.0
34	417702.0
35	454534.0
36	464412.0
37	446895.5
38	444367.5
39	466068.0
40	509597.5
41	568668.0
42	640277.5
43	710901.5
44	760677.0
45	793305.0
46	831197.5
47	821052.5
48	838794.5
49	944244.5
50	1090371.5
51	1140795.5
52	989884.5
53	783872.0
54	633744.5
55	536443.5
56	469406.0
57	419421.0
58	373448.5
59	319357.5
60	254575.5
61	195774.5
62	157518.5
63	129488.0
64	107495.5
65	92480.0
66	64440.5
67	34503.0
68	21821.0
69	17699.0
70	13407.0
71	9208.0
72	6209.5
73	4618.0
74	3195.0
75	2459.5
76	1801.5
77	968.5
78	381.0
79	236.0
80	186.5
81	160.0
82	238.0
83	302.5
84	297.5
85	202.5
86	86.5
87	85.0
88	48.5
89	11.0
90	3.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	33313.0
25	28460.0
26	31433.0
27	46231.0
28	52381.0
29	46627.0
30	85070.0
31	135694.0
32	188243.0
33	38236.0
34	28327.0
35	30635.0
36	35247.0
37	51133.0
38	25098.0
39	25399.0
40	25745.0
41	30714.0
42	50373.0
43	92741.0
44	266726.0
45	633607.0
46	1097851.0
47	1007520.0
48	1772606.0
49	1437857.0
50	2173398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.05718548881704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.66409781381894	6.309606558108519
2	12.368091368284222	2.2404019533037434
3	4.86187720115444	1.321047709041192
4	2.6465857073303964	0.9588247065337381
5	1.5919991477133533	0.720951578943924
6	1.019346909325008	0.5539448421125378
7	0.7511239770992283	0.47621484299799716
8	0.5776573352591777	0.41855597075345163
9	0.4544129985888724	0.3704132535074076
>10	3.7055417318416315	6.75052206313107
>50	0.5486411088281793	3.548336574897083
>100	1.4495870757361953	35.6937075984999
>500	0.2836900708579064	16.263485080312535
>1k	0.06622729877170637	10.63481401998082
>5k	0.007166386807386137	4.563229010400227
>10k+	0.0039538685833854555	9.175944237475841
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	77615	0.8195306243014615	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	48272	0.5097002164050782	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	43220	0.4563565494080933	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	40688	0.42962136238585147	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	31926	0.33710409987049483	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	26446	0.2792412148460536	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	26431	0.27908283103668013	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	18327	0.1935133382925064	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	17099	0.1805469837651316	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	14276	0.15073915084104442	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	12459	0.13155359206560469	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	12320	0.13008590209874385	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	10421	0.11003451183206248	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10271	0.10845067373832777	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	10104	0.1066873339939698	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10098	0.1066239804702204	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	9956	0.10512461374148488	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA	9723	0.10266438523588366	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	9623	0.10160849317339385	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9508	0.10039421730153057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.020822191472298936	0.0	0.0	0.0	0.0
2	0.03420034390404475	0.0	0.0	0.0	0.0
3	0.04998593023826732	0.0	0.0	0.0	0.0
4	0.07389132653303648	0.0	0.0	0.0	0.0
5	0.08974026639100845	0.0	0.0	0.0	0.0
6	0.14408703084735866	0.0	0.0	0.0	0.0
7	0.16689429939713843	0.0	0.0	0.0	0.0
8	0.1973567853999693	0.0	0.0	0.0	0.0
9	0.2306068264477732	0.0	0.0	0.0	0.0
10	0.25501905093253746	0.0	0.0	0.0	0.0
11	0.28207100557352627	0.0	0.0	0.0	0.0
12	0.3111080372919959	0.0	0.0	0.0	0.0
13	0.33714633555299445	0.0	0.0	0.0	0.0
14	0.36672187222333386	0.0	0.0	0.0	0.0
15	0.3945974226730647	0.0	0.0	0.0	0.0
16	0.4261369185796351	0.0	0.0	0.0	0.0
17	0.45888013143744394	0.0	0.0	0.0	0.0
18	0.4877165436640405	0.0	0.0	0.0	0.0
19	0.5176616425562514	0.0	0.0	0.0	0.0
20	0.5519992524284197	0.0	0.0	0.0	0.0
21	0.5811101965912636	0.0	0.0	0.0	0.0
22	0.6050895053304071	0.0	0.0	0.0	0.0
23	0.630779359210784	0.0	0.0	0.0	0.0
24	0.6582642295973936	0.0	0.0	0.0	0.0
25	0.6856329518571294	0.0	0.0	0.0	0.0
26	0.7169295925893272	0.0	0.0	0.0	0.0
27	0.7417219382165877	0.0	0.0	0.0	0.0
28	0.7718993333625463	0.0	0.0	0.0	0.0
29	0.8018549911753821	0.0	0.0	0.0	0.0
30	0.8298150129901121	0.0	0.0	0.0	0.0
31	0.856339021599856	0.0	0.0	0.0	0.0
32	0.8820077576389831	0.0	0.0	0.0	0.0
33	0.9098305134855894	0.0	0.0	0.0	0.0
34	0.939701699933426	0.0	0.0	0.0	0.0
35	0.9675138968594075	0.0	0.0	0.0	0.0
36	0.990553461662935	0.0	0.0	0.0	0.0
37	1.0064129604415317	0.0	0.0	0.0	0.0
38	1.0197805539526528	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGATA	125	0.0	154.61238	44
CATAGCG	145	0.0	151.46196	44
CTATGCG	65	0.0	148.66574	44
CCGTCAA	160	0.0	148.2434	44
CGTTAAT	30	3.5041012E-8	146.41324	44
CGAATAT	60	0.0	146.41324	44
ATACCCG	165	0.0	133.10294	44
ACGCAAT	185	0.0	128.21051	44
CATACGT	70	0.0	125.49706	44
AATCGCA	580	0.0	122.68419	44
CTATCAT	14340	0.0	118.3558	44
GCGGATT	215	0.0	102.14877	44
AACCGAG	125	0.0	98.389694	44
CGTCACG	65	0.0	97.57909	43
CGATTAC	145	0.0	96.93565	44
ACTTCTA	11145	0.0	95.76963	44
GTACGCT	45	9.094947E-12	93.96504	43
AACGACA	1580	0.0	89.65335	43
GCCGTCA	160	0.0	89.19338	43
ACCACCG	295	0.0	86.35899	44
>>END_MODULE