FastQCFastQC Report
Tue 24 May 2016
ERR841098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177435
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA177630.42521307931781105No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA162050.38791746610060956No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC128640.3079401594519125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA119890.2869942919518796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA112600.26954339205756644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA111690.267365021837563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA93860.22468332840606736No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT92820.2221937624403492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA87020.20830964455461304No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA86150.20622702687175262No Hit
AAAAAAAAAAAAAAAAAAAAAAAA75330.1803259655745691No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC73730.17649586408884876No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA69610.166633352763119No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC60200.14410756840022645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56730.13580103580307057No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG47620.11399339546875056No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC47050.11262892181446271No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA47020.11255710741160546No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT43150.10329304944301945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCA154.3176784E-5188.5569844
GACCTAT100.0032322395188.5569844
TTTAACG850.0177.465444
ACTCGCA650.0174.052644
ATAATTG1550.0170.3095244
GAGGATA850.0155.2822144
CCGTTGT850.0144.1906344
CGAACAC1400.0141.4177444
CGGTGCA550.0137.1323544
CGTGTCT650.0130.5394444
AATCGCA3100.0121.64966644
TCCGATC1800.0115.2292644
CTATCAT39700.0114.7015344
ACCGACC253.3034175E-4113.1341944
ATCGGTG1250.0113.1341944
CTTGATG1350.0111.7374744
ACGAACA3100.0106.4434644
ACTGACC850.0105.9059143
ACGTTCC750.0105.9059143
TATAGTC500.0105.9059143
Produced by FastQC (version 0.11.5)