FastQCFastQC Report
Tue 24 May 2016
ERR841097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046920
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT258322.4674282657700686No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC202321.932525885454476No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC141811.3545447598670386No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA114201.0908187827150118No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA110541.0558590914300998No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA79100.7555496121957742No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC61190.584476368776984No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT59820.5713903641156918No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA59130.564799602643946No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG57490.5491346043632751No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT34710.33154395751346805No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA32560.3110075268406373No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT31640.3022198448783097No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT30070.2872234745730333No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG26670.25474725862530084No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT26580.25388759408550793No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA22630.21615787261681885No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT19700.18817101593244948No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC19650.1876934245214534No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA18140.17327016390937225No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC17470.16687043900202497No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT17410.1662973293088297No Hit
ACTTCGCTGTGAAGATGGCGTCGGGCAGCGGGACAAAAAACTTGGACTTT16330.15598135483131473No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGA16180.15454858059832652No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC15710.15005922133496352No Hit
CATCACAAGACGTCTTGCACTCATGAGCTATCCCCACATTAGGCTTAAAA15340.14652504489359264No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.14576089863599895No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.14518778894280365No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG14980.14308638673442095No Hit
TACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT14950.14279983188782333No Hit
AGCGCAAAAGTTCTGAATGGCATCCGTCCTACCGGAAACCTAGGAAAAGG14720.14060291139724143No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC14420.13773736293126504No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCA14270.13630458869827686No Hit
AACTATAATGATTTTGGAAATTATAGTGGACAACAGCAATCAAATTA13970.13343904023230047No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.13200626599931228No Hit
CCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCC13800.13181522943491383No Hit
AGGAGCTATGGCTAAACATCATCCTGATTTGATCTTTTGCCGCAAGCAG13520.1291407175333359No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT13440.12837657127574217No Hit
CGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACAT13360.12761242501814846No Hit
ACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAAGC13330.12732587017155084No Hit
ACACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAA13210.12617965078516027No Hit
TTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGG13120.12531998624536736No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT13110.12522446796316813No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT12990.1240782485767776No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA12850.12274099262598862No Hit
CAGCTGCTGTAGCGAAGAGAGTTTGGCGCGATGTCTCACACCATTTT12830.12254995606159019No Hit
CATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGT12640.12073510869980515No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA12610.1204485538532075No Hit
TCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC12580.12016199900660987No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA12320.11767852366943034No Hit
AGTTGGTGAGGTAACATACGTGGAGCTCTTAATGGACGCTGAAGGAAAGT12240.11691437741183663No Hit
CATAATTGTGTGTGTATATATGCGTGTGTATACACACACACACACACTCA12210.11662782256523897No Hit
TAAACGCTCTCGGAATTATGGCGGCGG12140.11595919458984451No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG12130.1158636763076453No Hit
ACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGC12120.11576815802544606No Hit
TTGATACCCCAGGATTTGGAGATGCAGTGGATAATAGTAATTGCTGGCA12100.11557712146104764No Hit
AGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTATCGGAAAGCAAGC11900.1136667558170634No Hit
CACGTGGAATTCGCGAAGCTGCCAAAGCCTTAGACAAGCGCCAAGCCCAT11730.11204294501967677No Hit
ATTTTGGAAATTATAACCAGCAACCTTCTAACTACGGTCCAATGAAGAG11700.11175639017307912No Hit
ATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGG11570.11051465250448936No Hit
CGTGATTCCCGTGATTGCGTTACAAGCTTTGTCTCCTTCGACTTGGAGT11550.11032361594009094No Hit
CTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG11510.10994154281129408No Hit
AGAGACTGAGGGATGTGTCTACCAGCCGAGAAATGCCAGGAATGCCTC11330.10822221373170825No Hit
CACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGC11180.10678943949872005No Hit
CATTCACTCCGTCCTCCTGCCCTGTGGAGAAGGTGTCTGTTTCTCCT11170.10669392121652084No Hit
TACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTAAGGATGGTTT11150.10650288465212242No Hit
CCTGAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTT11030.10535666526573186No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACA10820.10335078133954839No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.10335078133954839No Hit
TTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT10790.10306422649295076No Hit
CCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT10780.10296870821075153No Hit
TCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTAT10750.1026821533641539No Hit
TGGCGCCTTATTCTAGGCACTTGTTGGGCAGAATGTCACACCTGCCGA10670.1019180071065602No Hit
ACGGTTCGTTTTTCCTTTAGTCAGGAAGGACGTTGGTGTTGAGGTTGGC10670.1019180071065602No Hit
ACTCTAGGTTCTCTGGCTTTGCTCTATAGTGCATTTGGTGTCATCATTG10660.10182248882436098No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT10600.1012493791311657No Hit
ATTACAGTATCTAACATTTTGCCTCCTCTTTTTTGGTTTTGCTGGTTAT10550.10077178772016965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATT400.0214.5298544
GAATCAC100.0021954738214.5298544
ATGAGCA700.0214.5298544
CCTTTGG700.0214.5298544
TGGGATA950.0214.5298544
ATTGCCA100.0021954738214.5298544
TGCACCG100.0021954738214.5298544
ACGCCCC550.0214.5298344
CTCCCAA600.0214.5298344
ATGAGTA550.0214.5298344
GAGCTAA650.0214.5298244
TACCTAC1000.0214.5298244
CACAGGG253.5615813E-9214.5298244
TTAGAGG850.0201.9104344
GACTCGC700.0199.2062844
ACGGGAC650.0198.0275344
TCATAAC500.0193.0768444
CTCGCCA950.0191.9477544
ACCAGAA1250.0188.7862544
ACTCATA401.8189894E-12187.7136144
Produced by FastQC (version 0.11.5)