FastQCFastQC Report
Tue 24 May 2016
ERR841096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5265267
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA207740.39454789282290903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA196010.37226982031490524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA191090.3629255648384023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA156980.2981425253458182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA155090.29455296379082013No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG134180.2548398780156828No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA133430.25341544882719147No Hit
AAAAAAAAAAAAAAAAAAAAAAAA125300.23797463642394584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103120.19584951722296323No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC87480.16614542054562476No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC83410.158415518149412No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT71900.13655527820336555No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA62660.1190063106011528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58480.11106749192396131No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA55590.10557869145097484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGT100.0028831332195.9007944
AACTGCG800.0195.9007944
ACATCGC450.0195.9007944
AGCGCCT2750.0192.3389644
GTTATAC1150.0187.3833844
GCGCAAA1850.0169.427744
GCGCATC1000.0166.5156744
CGCATTC550.0160.2824744
ACGACAG303.58535E-6130.6005444
GGTCGAG1550.0126.3876144
AGACGAT800.0122.43799644
GTCATAA651.8189894E-12120.5543444
GCTAGTT1750.0106.3461444
CCGTTGT4700.0104.20254544
AACGCCA1750.0100.7489844
ATACGAC750.0100.174943
TGCGTTA600.098.38605543
AATCCCG401.4980782E-597.9503944
AGCGGAT2500.097.9503944
CCAGAAT20100.096.9757644
Produced by FastQC (version 0.11.5)