##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841096.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5265267
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.33793632877497	39.0	37.0	39.0	34.0	39.0
2	37.867950286281776	39.0	38.0	39.0	35.0	39.0
3	38.0308419307131	39.0	38.0	39.0	35.0	39.0
4	38.53557929730819	39.0	38.0	40.0	35.0	41.0
5	39.180056775848215	40.0	38.0	41.0	35.0	41.0
6	39.1882111581426	40.0	38.0	41.0	35.0	41.0
7	39.179323099854194	40.0	38.0	41.0	35.0	41.0
8	39.09696203440395	40.0	38.0	41.0	35.0	41.0
9	39.08783524178356	40.0	38.0	41.0	35.0	41.0
10	39.06684067493633	40.0	38.0	41.0	35.0	41.0
11	38.99615442103886	40.0	38.0	41.0	35.0	41.0
12	38.88419694575793	40.0	38.0	41.0	35.0	41.0
13	38.83149002700148	40.0	38.0	41.0	35.0	41.0
14	38.78847872292136	40.0	38.0	41.0	35.0	41.0
15	38.734618396370024	40.0	38.0	41.0	35.0	41.0
16	38.79896745976985	40.0	38.0	41.0	35.0	41.0
17	38.81176168274087	40.0	38.0	41.0	35.0	41.0
18	38.84630105177952	40.0	38.0	41.0	35.0	41.0
19	38.863256127372075	40.0	38.0	41.0	35.0	41.0
20	38.89748041267423	40.0	38.0	41.0	35.0	41.0
21	38.908238081753495	40.0	38.0	41.0	35.0	41.0
22	38.88713070011454	40.0	38.0	41.0	35.0	41.0
23	38.86916807827599	40.0	38.0	41.0	35.0	41.0
24	38.76273188045354	40.0	38.0	41.0	35.0	41.0
25	38.79008027243515	40.0	38.0	41.0	35.0	41.0
26	38.68380521186827	40.0	38.0	41.0	35.0	41.0
27	38.6743439544588	40.0	38.0	41.0	35.0	41.0
28	38.660258070622724	40.0	38.0	41.0	35.0	41.0
29	38.633992375998844	40.0	38.0	41.0	35.0	41.0
30	38.648102807200004	40.0	38.0	41.0	35.0	41.0
31	38.596856400537554	40.0	38.0	41.0	35.0	41.0
32	38.44391120407454	40.0	38.0	41.0	34.0	41.0
33	38.40849349282023	40.0	38.0	41.0	34.0	41.0
34	38.433215634373546	40.0	37.0	41.0	34.0	41.0
35	38.47378673778614	40.0	37.0	41.0	34.0	41.0
36	38.456316260456305	40.0	37.0	41.0	34.0	41.0
37	38.42578800306419	40.0	37.0	41.0	34.0	41.0
38	38.359706504985496	40.0	37.0	41.0	34.0	41.0
39	38.321148490111106	40.0	37.0	41.0	34.0	41.0
40	38.251438455069795	40.0	36.0	41.0	34.0	41.0
41	38.16822791595244	40.0	36.0	41.0	34.0	41.0
42	38.08360461655507	40.0	36.0	41.0	34.0	41.0
43	37.994135151289534	39.0	36.0	41.0	34.0	41.0
44	37.83332415343418	39.0	35.0	41.0	34.0	41.0
45	37.62361401708144	39.0	35.0	41.0	33.0	41.0
46	37.394622767460476	39.0	35.0	41.0	33.0	41.0
47	37.132408172833536	38.0	35.0	40.0	32.0	41.0
48	37.084080935912425	38.0	35.0	40.0	32.0	41.0
49	37.0798128754342	38.0	35.0	40.0	32.0	41.0
50	36.68942117880966	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	29.0
21	73.0
22	145.0
23	330.0
24	584.0
25	995.0
26	1728.0
27	3382.0
28	6501.0
29	12617.0
30	24152.0
31	44234.0
32	79606.0
33	146335.0
34	247029.0
35	210887.0
36	344096.0
37	570132.0
38	759293.0
39	1184231.0
40	1628878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.25945179988023	19.25683920682465	29.483708993295117
2	24.79414624177653	23.98615302889673	30.008601653059568	21.211099076267168
3	20.842456802285618	24.334017629115483	28.921933113743332	25.901592454855564
4	22.1643650739839	24.423642713655358	32.44608488040587	20.965907331954867
5	15.58771853355205	26.750210388191142	32.34250038981879	25.31957068843802
6	20.677830772874387	27.593795338394045	30.179286254619186	21.549087634112382
7	25.426877687304366	26.58174789616557	24.247127448617515	23.74424696791255
8	22.288119481880024	26.929992344167918	27.139649328324662	23.642238845627393
9	22.650266358762053	26.515654381819576	27.630260725619422	23.20381853379895
10	22.11069258216155	24.783947328786933	28.13999745881833	24.965362630233187
11	24.233149050181122	25.653247973939404	26.28892323979012	23.824679736089358
12	23.003695729010516	27.836024269994287	26.487792546892685	22.672487454102516
13	24.519535286624592	27.077905071100854	25.887177231468034	22.515382410806517
14	20.211529633729874	28.861974141102436	27.454030346419277	23.47246587874841
15	20.529025403649996	29.591585763836857	28.480417042478567	21.39897179003458
16	24.316012844172956	30.50494875188666	23.409031298887598	21.77000710505279
17	19.940109399960154	32.979429153355376	23.392545905079455	23.687915541605012
18	21.527322356112236	29.48148688376107	23.444129234092024	25.54706152603467
19	23.778984047722556	27.516610268766996	23.867925406251953	24.836480277258495
20	24.61613057799348	26.02553678664349	26.208851327007725	23.149481308355302
21	22.9433759009752	29.124866792130387	25.10841330553607	22.823344001358336
22	24.373179935604405	29.70402070778177	24.057849298050794	21.864950058563032
23	25.76974728916881	28.3013757896798	24.038154190471253	21.890722730680135
24	22.388228365247194	28.80116051094845	25.90090872884509	22.909702394959268
25	24.54475181093123	26.860507892038783	26.870903846025218	21.723836451004765
26	22.493173164668796	30.726815092847076	26.44231037707855	20.33770136540558
27	24.954613812397024	27.82152903958437	26.705434804144957	20.518422343873652
28	24.062712390622202	28.41956143036376	24.845851415276357	22.67187476373768
29	25.125445937385386	29.822712601194247	24.77050200602363	20.281339455396736
30	26.5608395790313	29.99745913916758	24.4956030272943	18.94609825450682
31	25.161378716487555	26.745822448549383	24.911690191894056	23.181108643069003
32	21.671085839212893	28.905804860288075	27.00685310405057	22.41625619644846
33	22.657399394774554	25.037627990776507	29.610458939679763	22.694513674769173
34	23.414300338448385	27.054196027532356	26.28155175262438	23.24995188139488
35	20.25384948397407	26.024638835293928	28.974906869478307	24.746604811253693
36	23.584907077863303	26.592123753942275	27.747487385681225	22.075481782513194
37	22.025740254452224	28.591131266843572	24.161530923157084	25.22159755554712
38	22.402921079870243	26.99064449965584	27.120569964266707	23.48586445620721
39	21.53733153846873	26.932144855227612	27.74020356826099	23.790320038042665
40	20.152750072986457	29.585807854259244	28.080737641068964	22.18070443168533
41	20.72823042295078	29.009718124852903	27.243884041684545	23.018167410511776
42	21.462516686449725	30.296781056168616	25.985821360768966	22.254880896612693
43	21.550257366383295	28.011645100803957	27.828278611649925	22.60981892116282
44	22.132356233823227	26.267564953384024	26.159622773314705	25.440456039478043
45	20.565751624546397	26.477172325178255	28.235823513603414	24.721252536671937
46	21.71820423752798	27.719639057271788	26.140628536104142	24.42152816909609
47	23.17702573719862	28.029641968069768	27.130593761144965	21.66273853358664
48	23.02510659968734	27.1757426489246	26.002729093951455	23.796421657436603
49	18.041253403030154	30.28262958712923	25.482459662274348	26.19365734756627
50	19.92370329141771	30.293308587773815	28.673350109663527	21.109638011144952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176284.0
1	176126.0
2	128119.5
3	78627.0
4	74151.5
5	54213.5
6	26738.5
7	16965.5
8	17613.5
9	16846.5
10	15192.0
11	13422.5
12	11714.5
13	10427.5
14	10820.5
15	11930.5
16	12885.0
17	14717.5
18	15579.0
19	16238.0
20	17549.5
21	21964.5
22	28442.0
23	33906.5
24	39544.5
25	46460.0
26	54496.5
27	66052.0
28	75810.0
29	82895.0
30	97151.5
31	112549.0
32	128084.5
33	143737.0
34	174059.0
35	217786.0
36	238759.0
37	241270.0
38	244377.5
39	247138.0
40	256641.0
41	276362.5
42	301314.0
43	328941.5
44	350199.5
45	354806.0
46	370562.5
47	397632.5
48	437518.5
49	483092.5
50	514697.0
51	527838.0
52	516158.5
53	472165.0
54	400521.5
55	347215.5
56	315393.0
57	296506.5
58	288645.0
59	255473.0
60	197055.5
61	157031.0
62	136670.0
63	113704.0
64	90224.5
65	76044.0
66	54922.0
67	32367.5
68	21002.5
69	14403.0
70	10838.5
71	8486.5
72	6856.0
73	4456.0
74	2312.0
75	1735.5
76	1308.0
77	818.0
78	487.0
79	324.5
80	216.5
81	138.5
82	71.0
83	273.5
84	484.5
85	475.5
86	466.0
87	233.5
88	3.0
89	2.0
90	2.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	32462.0
25	39696.0
26	45067.0
27	55715.0
28	53503.0
29	52325.0
30	67170.0
31	96405.0
32	102369.0
33	23827.0
34	19153.0
35	18782.0
36	22009.0
37	22600.0
38	15137.0
39	16005.0
40	19816.0
41	20618.0
42	27061.0
43	49267.0
44	146151.0
45	369807.0
46	554731.0
47	577806.0
48	887520.0
49	872028.0
50	1058237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.057793682910564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70584898436967	8.039662776100124
2	11.243120442825806	2.4864821241776367
3	4.370698029659901	1.4499083118684875
4	2.350398030486236	1.0396086597534453
5	1.3789989651533396	0.7624343022806402
6	0.9508418913764107	0.6308528075945283
7	0.6875046627442729	0.5321599301665625
8	0.5146812241422458	0.4552991031226242
9	0.41578622802837223	0.41379104931600125
>10	3.2488690747282485	7.315751325040782
>50	0.5384876845984886	4.2797629894236495
>100	1.3199223407533192	38.75919331160853
>500	0.2164808363497612	15.156808889440995
>1k	0.05225399498097684	11.25581468643383
>5k	0.00305380490148566	2.1585851446879403
>10k+	0.00305380490148566	5.263884588984199
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	20774	0.39454789282290903	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	19601	0.37226982031490524	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	19109	0.3629255648384023	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	15698	0.2981425253458182	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15509	0.29455296379082013	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	13418	0.2548398780156828	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	13343	0.25341544882719147	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	12530	0.23797463642394584	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10312	0.19584951722296323	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8748	0.16614542054562476	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	8341	0.158415518149412	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7190	0.13655527820336555	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	6266	0.1190063106011528	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5848	0.11106749192396131	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	5559	0.10557869145097484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10159028972319922	0.0	0.0	0.0	0.0
2	0.15463603270261508	0.0	0.0	0.0	0.0
3	0.20779573001711024	0.0	0.0	0.0	0.0
4	0.267241908150147	0.0	0.0	0.0	0.0
5	0.3068220472010251	0.0	0.0	0.0	0.0
6	0.389647856414499	0.0	0.0	0.0	0.0
7	0.4352865676137601	0.0	0.0	0.0	0.0
8	0.4892249528846305	0.0	0.0	0.0	0.0
9	0.5486141538501277	0.0	0.0	0.0	0.0
10	0.5924865728556595	0.0	0.0	0.0	0.0
11	0.6435760997495473	0.0	0.0	0.0	0.0
12	0.6913229661477756	0.0	0.0	0.0	0.0
13	0.7349294917047892	0.0	0.0	0.0	0.0
14	0.7793906747748974	0.0	0.0	0.0	0.0
15	0.8303852397228858	0.0	0.0	0.0	7.596955671953578E-5
16	0.8727192752048472	0.0	0.0	0.0	7.596955671953578E-5
17	0.9279681353291296	0.0	0.0	0.0	7.596955671953578E-5
18	0.9583369656277639	0.0	0.0	0.0	7.596955671953578E-5
19	0.9932829617187504	0.0	0.0	0.0	7.596955671953578E-5
20	1.0317045650296557	0.0	0.0	0.0	7.596955671953578E-5
21	1.0664036600613036	0.0	0.0	0.0	7.596955671953578E-5
22	1.095993802403563	0.0	0.0	0.0	7.596955671953578E-5
23	1.1249761882920657	0.0	0.0	0.0	7.596955671953578E-5
24	1.1545853230235048	0.0	0.0	0.0	7.596955671953578E-5
25	1.1872332400237253	0.0	0.0	0.0	7.596955671953578E-5
26	1.2198621646347658	0.0	0.0	0.0	7.596955671953578E-5
27	1.248350748404592	0.0	0.0	0.0	7.596955671953578E-5
28	1.2767253778393384	0.0	0.0	0.0	7.596955671953578E-5
29	1.3084236753805647	0.0	0.0	0.0	7.596955671953578E-5
30	1.3382227340038026	0.0	0.0	0.0	7.596955671953578E-5
31	1.3666353482169091	0.0	0.0	0.0	7.596955671953578E-5
32	1.3962254905591682	0.0	0.0	0.0	7.596955671953578E-5
33	1.4248660134424331	0.0	0.0	0.0	7.596955671953578E-5
34	1.4528418027044023	0.0	0.0	0.0	7.596955671953578E-5
35	1.4813873636417678	0.0	0.0	0.0	7.596955671953578E-5
36	1.505887545683818	0.0	0.0	0.0	7.596955671953578E-5
37	1.5210814570277253	0.0	0.0	0.0	7.596955671953578E-5
38	1.5350788478532997	0.0	0.0	0.0	7.596955671953578E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGT	10	0.0028831332	195.90079	44
AACTGCG	80	0.0	195.90079	44
ACATCGC	45	0.0	195.90079	44
AGCGCCT	275	0.0	192.33896	44
GTTATAC	115	0.0	187.38338	44
GCGCAAA	185	0.0	169.4277	44
GCGCATC	100	0.0	166.51567	44
CGCATTC	55	0.0	160.28247	44
ACGACAG	30	3.58535E-6	130.60054	44
GGTCGAG	155	0.0	126.38761	44
AGACGAT	80	0.0	122.437996	44
GTCATAA	65	1.8189894E-12	120.55434	44
GCTAGTT	175	0.0	106.34614	44
CCGTTGT	470	0.0	104.202545	44
AACGCCA	175	0.0	100.74898	44
ATACGAC	75	0.0	100.1749	43
TGCGTTA	60	0.0	98.386055	43
AATCCCG	40	1.4980782E-5	97.95039	44
AGCGGAT	250	0.0	97.95039	44
CCAGAAT	2010	0.0	96.97576	44
>>END_MODULE