FastQCFastQC Report
Tue 24 May 2016
ERR841095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840624
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC201401.094194142855901No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT113500.6166387051347805No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA109620.5955588974173975No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA100610.5466081068159494No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT64450.350152991594155No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC58250.3164687627674093No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA56630.3076673997513886No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG55140.299572318952703No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC54330.29517163744469266No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG52160.2833821573553317No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC51870.2818066047166613No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT47020.2554568450699328No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.25458757464859744No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA45660.24806804648858216No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.23709350741922303No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT41720.22666226236319859No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG41050.2230221924738567No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA36420.19786767965646432No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT35640.19362998635245438No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG32740.17787445996575074No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT31530.17130060240440198No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.16858413233772893No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG30800.16733455610705936No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT30650.16651961508705743No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA29070.15793556967637062No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTC28730.15608837003103296No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT26600.1445162075470058No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA25750.13989820843366163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA25450.1382683263936578No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC25310.13750771477498935No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG25010.13587783273498552No Hit
ATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGTTTGT24390.13250940985231097No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA24330.1321834334443102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA23680.12865202235763523No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC22970.12479463486295952No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC22450.12196950599361955No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT22400.12169785898695225No Hit
AGGGCTTCCTTCTAAGGTGCAGGATACTGTGGAGGAGAGCAGTGAGAGCA22250.12088291796695035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA21910.11903571832161267No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTT21810.11849242430827807No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC20990.11403741339893428No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA20130.10936508488425664No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC19270.10469275636957902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18610.1011070158815706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA18610.1011070158815706No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC18490.10045506306556908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCT750.0169.9922244
GGGTCCC209.646737E-7169.992244
AGGGTAT100.004406466169.992244
GACCCCC650.0169.992244
CGCTATC600.0169.992244
ATCTTAT400.0169.992244
GGGTTAC100.004406466169.992244
ACCCGAG100.004406466169.992244
TGCACAA650.0169.992244
ACGACTA302.1464075E-10169.992244
GCTCATA302.1464075E-10169.992244
TCAAATG209.646737E-7169.992244
AGCACTA100.004406466169.992244
CCAGCGT1300.0156.9158844
AGTGATA750.0147.3265844
GCCCTCA700.0145.7076144
CCCCAGC356.2755134E-10145.7076144
CCGCCTC650.0143.8395744
GTCTAAT304.2613465E-8141.6601744
CGAATGC900.0141.6601744
Produced by FastQC (version 0.11.5)