##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841095.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1840624
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.1770209450708	39.0	37.0	39.0	32.0	39.0
2	37.78487458600996	39.0	38.0	39.0	35.0	39.0
3	37.98015075322282	39.0	38.0	39.0	35.0	39.0
4	38.42009611957684	39.0	38.0	40.0	35.0	41.0
5	39.1055125870357	40.0	38.0	41.0	35.0	41.0
6	39.08301152217943	40.0	38.0	41.0	35.0	41.0
7	38.992033679882475	40.0	38.0	41.0	35.0	41.0
8	38.878827506323944	40.0	38.0	41.0	35.0	41.0
9	38.806977959648464	40.0	38.0	41.0	35.0	41.0
10	38.643588804666244	40.0	38.0	41.0	35.0	41.0
11	38.55485911299646	40.0	37.0	41.0	35.0	41.0
12	38.34290925251437	40.0	37.0	41.0	34.0	41.0
13	38.11573357730857	40.0	37.0	41.0	34.0	41.0
14	38.112672115543425	40.0	36.0	41.0	34.0	41.0
15	38.01426201114405	39.0	36.0	41.0	34.0	41.0
16	37.97189214092612	39.0	36.0	41.0	34.0	41.0
17	38.03451275219708	39.0	36.0	41.0	34.0	41.0
18	37.96856120533037	39.0	36.0	41.0	34.0	41.0
19	37.93080009822756	39.0	35.0	41.0	34.0	41.0
20	37.82026910439068	39.0	35.0	41.0	34.0	41.0
21	37.73425370961152	39.0	35.0	41.0	34.0	41.0
22	37.72286192073992	39.0	35.0	41.0	34.0	41.0
23	37.639771620928556	39.0	35.0	41.0	34.0	41.0
24	37.38993895548466	38.0	35.0	41.0	34.0	41.0
25	37.348993398529004	38.0	35.0	41.0	34.0	41.0
26	37.23599512582921	38.0	35.0	41.0	33.0	41.0
27	37.15559153098632	38.0	35.0	40.0	33.0	41.0
28	37.05357267043839	37.0	35.0	40.0	33.0	41.0
29	36.891891207811526	37.0	35.0	40.0	33.0	41.0
30	36.81251897911517	37.0	35.0	40.0	33.0	41.0
31	36.672055196147724	37.0	35.0	40.0	33.0	41.0
32	36.548240834790036	36.0	35.0	40.0	33.0	41.0
33	36.33711624490484	36.0	35.0	40.0	33.0	41.0
34	36.299416034597996	36.0	35.0	39.0	33.0	41.0
35	36.2862322455973	36.0	35.0	39.0	33.0	41.0
36	36.22702035478805	36.0	35.0	39.0	33.0	41.0
37	36.15680135862625	35.0	35.0	39.0	33.0	41.0
38	35.98217815549649	35.0	35.0	39.0	33.0	41.0
39	35.847453815838215	35.0	35.0	39.0	33.0	41.0
40	35.84166097060834	35.0	35.0	38.0	33.0	41.0
41	35.72797115132016	35.0	35.0	37.0	33.0	40.0
42	35.61068765440919	35.0	35.0	37.0	33.0	40.0
43	35.477996710225135	35.0	35.0	37.0	33.0	39.0
44	35.30276277576807	35.0	35.0	37.0	33.0	39.0
45	35.14419468782972	35.0	35.0	37.0	32.0	39.0
46	34.82888022061604	35.0	34.0	36.0	31.0	39.0
47	34.66952742112584	35.0	34.0	36.0	31.0	39.0
48	34.60497803835103	35.0	34.0	36.0	31.0	39.0
49	34.569147123516835	35.0	34.0	36.0	31.0	38.0
50	34.23919758490989	35.0	34.0	36.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	8.0
22	35.0
23	88.0
24	218.0
25	452.0
26	987.0
27	2244.0
28	4565.0
29	8882.0
30	15457.0
31	25875.0
32	42850.0
33	73993.0
34	136409.0
35	214713.0
36	260824.0
37	303121.0
38	307030.0
39	282486.0
40	160379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.96938646893662	18.69295412859987	29.337659402463512
2	22.627652361373098	21.244099827015187	33.41839506602109	22.709852745590624
3	23.117920879006252	19.969532071732196	30.165965455193454	26.746581594068097
4	20.088187484244475	23.869622475856016	33.833091386399396	22.209098653500117
5	15.727818392023574	25.0573175184068	35.58912629629952	23.62573779327011
6	19.696363841827555	25.15451281739236	31.819263467172004	23.32985987360808
7	24.326152435261086	23.803014629821188	28.427207294917377	23.44362564000035
8	24.038369596397743	23.396793696050906	30.831826597936352	21.733010109615
9	20.840975669120905	27.852619546414694	29.94647467380627	21.359930110658123
10	23.779218352037134	24.287958866123663	31.143514373386417	20.789308408452786
11	24.841358148106295	24.930893001503836	27.134547848990344	23.093201001399525
12	23.726029868131675	25.370472187692872	27.32361416563079	23.579883778544666
13	22.654056450421162	26.50247959387686	26.920924642947174	23.922539312754804
14	19.080648736515442	26.156998930797382	28.846141308599694	25.91621102408748
15	23.055170420466105	29.00891219499474	29.80320804248994	18.132709342049218
16	23.812196298646544	30.52258364554629	25.716822121193683	19.948397934613478
17	19.74765079668634	35.98926233712045	25.165324368257718	19.097762497935484
18	21.195583671624405	27.186052121454463	26.348618729300497	25.26974547762063
19	23.677350724536897	26.46059162544876	24.316862107633064	25.545195542381276
20	23.10520779909422	21.90947200514608	32.11150131694469	22.873818878815012
21	21.198897765105748	28.14371647875938	29.037978424708143	21.619407331426736
22	25.82097158354993	23.648121506619493	27.608028581611453	22.922878328219127
23	25.854438494771337	24.70162292787663	30.724960665513436	18.718977911838593
24	21.40833760724624	29.315275689114127	27.718534583923716	21.55785211971592
25	23.70708907344614	26.258838818178344	27.865360569178936	22.168711539196575
26	22.789162486441587	31.21482836433823	27.012803750982762	18.98320539823742
27	24.50721384634739	27.451626288108024	26.077331726577896	21.963828138966687
28	25.65426121711864	24.638521338215174	27.552827431041017	22.154390013625168
29	23.735787185335386	28.73031720274371	26.22565576513666	21.30823984678424
30	24.87755455342674	28.90087154934854	29.183365923016453	17.03820797420827
31	23.131301338783782	28.401148597005893	27.777736242514305	20.689813821696017
32	20.053644284759496	30.46786477321471	27.439151408675915	22.03933953334988
33	20.57974941146012	22.85431304902793	34.12869003967752	22.437247499834427
34	22.08918667199105	28.27363296529232	28.77597531069177	20.86120505202485
35	18.95833130518589	24.47107942874388	30.810155664372424	25.7604336016978
36	22.231877150207456	25.70722347491857	27.745635422586425	24.315263952287548
37	20.820848969487397	31.21905515059827	24.273103031612866	23.686992848301472
38	24.45328878386493	28.098009653344818	26.008241322511942	21.440460240278306
39	20.216713394924604	30.54101535156458	27.027599612958696	22.214671640552115
40	21.027713912881378	27.15857826384142	27.625427204374574	24.188280618902628
41	20.1296168838908	28.13619881577642	28.249017671001997	23.48516662933078
42	18.95602777140608	30.75636845872206	27.482799552268176	22.80480421760368
43	20.37052589040188	26.330764446436973	28.470886123906435	24.827823539254716
44	23.177446884393945	25.215901564171805	27.54547307280787	24.06117847862638
45	21.8839003099749	26.28600263445899	28.769996002437857	23.060101053128257
46	19.94255144662426	29.52000147303983	27.324959668452724	23.21248741188318
47	20.301206663583056	30.929907930153682	30.068123577311116	18.700761828952146
48	25.719251269551872	26.77519292732215	23.24176026443104	24.263795538694936
49	21.0695667602635	28.80102177323281	26.301930047399235	23.82748141910446
50	18.03075831121135	31.049690992675817	27.594225637218162	23.325325058894673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23440.0
1	24382.5
2	33444.0
3	40821.5
4	38245.0
5	25728.0
6	10979.5
7	7400.5
8	8090.5
9	8050.0
10	7416.0
11	6383.0
12	5207.5
13	4939.5
14	6134.5
15	7013.5
16	6430.0
17	4836.5
18	3925.0
19	4596.0
20	5516.0
21	8311.0
22	12581.0
23	16014.5
24	17045.5
25	19185.0
26	24351.5
27	27578.0
28	31176.5
29	36154.5
30	39551.5
31	50650.0
32	76019.5
33	88197.0
34	81725.0
35	81201.5
36	84901.5
37	89453.5
38	93442.0
39	96941.5
40	104284.0
41	108142.0
42	105851.0
43	106873.0
44	114005.5
45	119611.5
46	126593.0
47	136070.5
48	152168.5
49	164900.0
50	179938.0
51	196461.0
52	181472.5
53	154781.0
54	136534.0
55	118453.5
56	106205.5
57	99640.5
58	91455.5
59	77132.5
60	60312.5
61	45417.5
62	33183.0
63	25793.0
64	20411.0
65	16382.0
66	10933.5
67	6070.0
68	4493.0
69	4349.0
70	4070.0
71	3037.5
72	2143.0
73	1413.0
74	585.0
75	285.5
76	154.5
77	57.0
78	20.5
79	15.0
80	9.5
81	4.0
82	2.0
83	1.5
84	2.5
85	3.0
86	2.0
87	1.0
88	0.5
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9214.0
25	8741.0
26	10321.0
27	14939.0
28	19524.0
29	16080.0
30	22988.0
31	34628.0
32	43299.0
33	10621.0
34	6733.0
35	7066.0
36	7766.0
37	8333.0
38	6073.0
39	4998.0
40	6731.0
41	6971.0
42	11903.0
43	13535.0
44	44230.0
45	113884.0
46	203507.0
47	176285.0
48	322116.0
49	281831.0
50	428307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.921147071812501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.5268539314276	4.742844344280232
2	11.815518987128552	1.6355388927941958
3	4.943325173133442	1.0264044164114865
4	2.551910159411143	0.7064858210934802
5	1.6666446339616492	0.5767546314047843
6	1.1255803909966338	0.46741844563415536
7	0.771067248780344	0.373566888174662
8	0.6174266759105596	0.3418640664029836
9	0.4662390260413378	0.2904217982865646
>10	4.025841811800476	5.896476111395644
>50	0.9245111397985389	4.590032432761328
>100	1.8561828650172043	32.19625124967458
>500	0.5521606979519674	26.49388416222212
>1k	0.14798543201594516	15.607211592828804
>5k	0.00556934421565385	2.163160506942154
>10k+	0.003182482408945057	2.8916846396928273
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	20140	1.094194142855901	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11350	0.6166387051347805	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10962	0.5955588974173975	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10061	0.5466081068159494	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	6445	0.350152991594155	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5825	0.3164687627674093	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5663	0.3076673997513886	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	5514	0.299572318952703	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5433	0.29517163744469266	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	5216	0.2833821573553317	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	5187	0.2818066047166613	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4702	0.2554568450699328	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4686	0.25458757464859744	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	4566	0.24806804648858216	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4364	0.23709350741922303	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	4172	0.22666226236319859	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4105	0.2230221924738567	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3642	0.19786767965646432	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	3564	0.19362998635245438	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3274	0.17787445996575074	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	3153	0.17130060240440198	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3103	0.16858413233772893	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	3080	0.16733455610705936	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	3065	0.16651961508705743	No Hit
ACCATTTGGTGAGCTGAGGGTGAAGCTACAAAGGTTGAAGCGTTCTTCA	2907	0.15793556967637062	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTC	2873	0.15608837003103296	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	2660	0.1445162075470058	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	2575	0.13989820843366163	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2545	0.1382683263936578	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2531	0.13750771477498935	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2501	0.13587783273498552	No Hit
ATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGTTTGT	2439	0.13250940985231097	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2433	0.1321834334443102	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2368	0.12865202235763523	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	2297	0.12479463486295952	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	2245	0.12196950599361955	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	2240	0.12169785898695225	No Hit
AGGGCTTCCTTCTAAGGTGCAGGATACTGTGGAGGAGAGCAGTGAGAGCA	2225	0.12088291796695035	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2191	0.11903571832161267	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTT	2181	0.11849242430827807	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2099	0.11403741339893428	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	2013	0.10936508488425664	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1927	0.10469275636957902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1861	0.1011070158815706	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1861	0.1011070158815706	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	1849	0.10045506306556908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03732429871608759	0.0	0.0	0.0	0.0
2	0.05932770625613922	0.0	0.0	0.0	0.0
3	0.08306965463886161	0.0	0.0	0.0	0.0
4	0.11778614209094307	0.0	0.0	0.0	0.0
5	0.1442445605403385	0.0	0.0	0.0	0.0
6	0.20574544284981616	0.0	0.0	0.0	0.0
7	0.2361699075965542	0.0	0.0	0.0	0.0
8	0.2782208642286529	0.0	0.0	0.0	0.0
9	0.3311920305287772	0.0	0.0	0.0	0.0
10	0.357813437182173	0.0	0.0	0.0	0.0
11	0.38997644277158183	0.0	0.0	0.0	0.0
12	0.42170481315032293	0.0	0.0	0.0	0.0
13	0.4557150183850694	0.0	0.0	0.0	0.0
14	0.48825832978381245	0.0	0.0	0.0	0.0
15	0.5213992645972235	0.0	0.0	0.0	5.432940133346082E-5
16	0.5502481767052912	0.0	0.0	0.0	5.432940133346082E-5
17	0.5893110162640496	0.0	0.0	0.0	5.432940133346082E-5
18	0.6168560227401142	0.0	0.0	0.0	5.432940133346082E-5
19	0.6513551925868618	0.0	0.0	0.0	5.432940133346082E-5
20	0.6885708325002825	0.0	0.0	0.0	5.432940133346082E-5
21	0.7156812037656795	0.0	0.0	0.0	5.432940133346082E-5
22	0.7395318109510688	0.0	0.0	0.0	5.432940133346082E-5
23	0.7675114526378011	0.0	0.0	0.0	5.432940133346082E-5
24	0.7938068828831961	0.0	0.0	0.0	5.432940133346082E-5
25	0.8201023131285912	0.0	0.0	0.0	5.432940133346082E-5
26	0.8544928241726719	0.0	0.0	0.0	5.432940133346082E-5
27	0.8781261137527273	0.0	0.0	0.0	5.432940133346082E-5
28	0.9082246020914646	0.0	0.0	0.0	5.432940133346082E-5
29	0.9384860786342023	0.0	0.0	0.0	5.432940133346082E-5
30	0.9669003555316024	0.0	0.0	0.0	5.432940133346082E-5
31	0.9928154799676632	0.0	0.0	0.0	5.432940133346082E-5
32	1.01992585123306	0.0	0.0	0.0	5.432940133346082E-5
33	1.0463842696824555	0.0	0.0	0.0	5.432940133346082E-5
34	1.0768087344291937	0.0	0.0	0.0	5.432940133346082E-5
35	1.1028325176679212	0.0	0.0	0.0	5.432940133346082E-5
36	1.1261941602413095	0.0	0.0	0.0	5.432940133346082E-5
37	1.1413520632133451	0.0	0.0	0.0	5.432940133346082E-5
38	1.156075330974713	0.0	0.0	0.0	5.432940133346082E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCT	75	0.0	169.99222	44
GGGTCCC	20	9.646737E-7	169.9922	44
AGGGTAT	10	0.004406466	169.9922	44
GACCCCC	65	0.0	169.9922	44
CGCTATC	60	0.0	169.9922	44
ATCTTAT	40	0.0	169.9922	44
GGGTTAC	10	0.004406466	169.9922	44
ACCCGAG	10	0.004406466	169.9922	44
TGCACAA	65	0.0	169.9922	44
ACGACTA	30	2.1464075E-10	169.9922	44
GCTCATA	30	2.1464075E-10	169.9922	44
TCAAATG	20	9.646737E-7	169.9922	44
AGCACTA	10	0.004406466	169.9922	44
CCAGCGT	130	0.0	156.91588	44
AGTGATA	75	0.0	147.32658	44
GCCCTCA	70	0.0	145.70761	44
CCCCAGC	35	6.2755134E-10	145.70761	44
CCGCCTC	65	0.0	143.83957	44
GTCTAAT	30	4.2613465E-8	141.66017	44
CGAATGC	90	0.0	141.66017	44
>>END_MODULE