FastQCFastQC Report
Tue 24 May 2016
ERR841094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656127
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC101650.6137814310134427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA84840.5122795534400442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA81630.49289698193435644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA78860.47617121150733005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69290.4183857880464481No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG68380.41289104036103513No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT64080.38692684800139115No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA60730.3666989306979477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA60390.3646459480462549No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC56230.3395271014843668No Hit
AAAAAAAAAAAAAAAAAAAAAAAA52550.3173065833719274No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC45240.27316745636053275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38670.2334965857087047No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC35640.2152008873715603No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC34900.21073263101199363No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT34200.2065059020232144No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA31710.19147082319169967No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA30950.18688180314673936No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCCC30560.18452691128156234No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA30320.18307774705683802No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT29270.17673765357366916No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG28930.1746846709219764No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC25370.1531887349218991No Hit
CACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAAGGCCTTCAC24910.15041117015784417No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC23990.1448560406297343No Hit
AGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTTGTGCCAGC22480.13573838238251051No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22220.1341684544723925No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT22140.13368539973081775No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA21940.13247776287688082No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.1313908897083376No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAACA21310.1286737067869795No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT21100.12740568809034575No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.12185055856223587No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA20040.12100521276448002No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT19490.11768421141615348No Hit
TCTGCACTTCGGCTGCTCTCGGGTTAGCACCCTATGGTGCCTTCTCTT19430.1173219203599724No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG19270.11635581087682284No Hit
CCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA19230.11611428350603546No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT18340.11074029950601613No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA18310.1105591539779256No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA18190.10983457186556345No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT18160.10965342633747291No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT18100.10929113528129185No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA18030.10886846238241392No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC17710.10693624341611484No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC17690.10681547973072113No Hit
TCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGG17510.1057286065621779No Hit
ACCCAGCGAACAATGAACCCTCTCTACTCATTTTCCTGTTCCTCTAAAT17280.10433982418015045No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC16850.10174340494418603No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA16820.10156225941609551No Hit
CCATCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGTCGG16690.1007772954610365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACA450.0261.812244
CGTGACT151.1647371E-5261.812244
GCTATGT100.0012095904261.812244
TGTATAC251.0822987E-9261.812244
GATAGTA151.1647371E-5261.812244
GATGTCA650.0261.8121644
GCACGGG1150.0261.8121644
GGTCAAT850.0261.8121644
TGTTAAC650.0261.8121644
CTCATGA1650.0245.9447844
TCGTGAC750.0244.3580344
TACTAGT750.0244.3580344
ACGAACA1550.0236.4755244
ACATAGC900.0232.7219444
GTAAAGG900.0232.7219444
ACCTGGA1150.0227.6627744
CCATTAC253.406858E-7209.4497544
TGTACCA1450.0207.6441544
TAAATCT1600.0204.5407744
CTATCAT8350.0202.2381744
Produced by FastQC (version 0.11.5)