##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841094.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1656127
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.38572524933172	39.0	35.0	39.0	31.0	39.0
2	37.10564105289027	39.0	35.0	39.0	33.0	39.0
3	37.30207224445952	39.0	35.0	39.0	34.0	39.0
4	37.956865626851084	39.0	35.0	40.0	35.0	41.0
5	38.50547391594968	40.0	36.0	41.0	35.0	41.0
6	38.59374854706191	40.0	36.0	41.0	35.0	41.0
7	38.524421738187954	40.0	36.0	41.0	35.0	41.0
8	38.45912541731401	40.0	36.0	41.0	35.0	41.0
9	38.45830845098232	40.0	36.0	41.0	35.0	41.0
10	38.378719747942036	40.0	36.0	41.0	34.0	41.0
11	38.35219340062688	40.0	36.0	41.0	34.0	41.0
12	38.17075441678084	40.0	35.0	41.0	34.0	41.0
13	38.09960468007586	40.0	35.0	41.0	34.0	41.0
14	38.15407453655426	40.0	35.0	41.0	34.0	41.0
15	38.04270505824734	40.0	35.0	41.0	34.0	41.0
16	38.03770423403519	40.0	35.0	41.0	34.0	41.0
17	38.03254339793989	40.0	35.0	41.0	34.0	41.0
18	38.02216738209086	40.0	35.0	41.0	34.0	41.0
19	38.01850160042074	40.0	35.0	41.0	34.0	41.0
20	37.962435248021436	40.0	35.0	41.0	34.0	41.0
21	37.94606150373733	40.0	35.0	41.0	34.0	41.0
22	37.95380245597107	40.0	35.0	41.0	34.0	41.0
23	37.87640078327326	40.0	35.0	41.0	34.0	41.0
24	37.74096551774109	39.0	35.0	41.0	34.0	41.0
25	37.7229616229487	39.0	35.0	41.0	34.0	41.0
26	37.66744308441388	39.0	35.0	41.0	34.0	41.0
27	37.66404322647093	39.0	35.0	41.0	34.0	41.0
28	37.63685106142053	39.0	35.0	41.0	34.0	41.0
29	37.49521057942129	39.0	35.0	41.0	33.0	41.0
30	37.5136276498208	39.0	35.0	41.0	34.0	41.0
31	37.44151036914525	39.0	35.0	41.0	33.0	41.0
32	37.19008327937904	38.0	35.0	41.0	33.0	41.0
33	37.148943811195934	38.0	35.0	41.0	33.0	41.0
34	37.151068453985175	38.0	35.0	41.0	33.0	41.0
35	37.141286442118165	38.0	35.0	41.0	33.0	41.0
36	37.14912485821482	38.0	35.0	41.0	33.0	41.0
37	37.11901260622116	37.0	35.0	41.0	34.0	41.0
38	36.9510433652769	37.0	35.0	40.0	33.0	41.0
39	36.94468810881705	37.0	35.0	40.0	33.0	41.0
40	36.85675059126596	37.0	35.0	40.0	33.0	41.0
41	36.736451605997246	36.0	35.0	40.0	33.0	41.0
42	36.61961484996679	36.0	35.0	40.0	33.0	41.0
43	36.54731524862501	36.0	35.0	40.0	33.0	41.0
44	36.36145314564023	36.0	35.0	40.0	33.0	41.0
45	36.243990269942685	36.0	35.0	39.0	33.0	41.0
46	35.97027444371288	35.0	35.0	39.0	32.0	41.0
47	35.711540854894835	35.0	35.0	39.0	31.0	41.0
48	35.74305976169866	35.0	34.0	39.0	31.0	41.0
49	35.640186629677004	35.0	34.0	39.0	31.0	41.0
50	36.243831657681284	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	4.0
21	13.0
22	45.0
23	93.0
24	250.0
25	585.0
26	1171.0
27	2237.0
28	4487.0
29	8281.0
30	14558.0
31	24917.0
32	40756.0
33	79525.0
34	227762.0
35	84882.0
36	123799.0
37	190493.0
38	237839.0
39	280697.0
40	333732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.04904877464108	19.41028677148552	33.540664453873404
2	22.842571855902357	22.146188064079627	30.61613028469435	24.39510979532367
3	21.675451218415013	21.14554016690749	30.35842058006421	26.820588034613284
4	18.49314696276312	24.56514506435799	34.983548966957244	21.958159005921647
5	15.513846462258027	27.285105550480125	34.09889458960575	23.102153397656096
6	21.78444044448282	26.670478773668925	27.884395339246325	23.660685442601924
7	24.713744779234926	26.375936144993712	25.976087582655193	22.934231493116165
8	25.2675066586077	27.56672646481822	26.154214018610894	21.011552857963185
9	25.600029466339237	24.17332728709815	26.10941069132983	24.117232555232782
10	23.263252153971283	25.25838900036048	28.405973696461682	23.072385149206553
11	27.48835083299771	23.5769358267814	26.287597509128226	22.647115831092666
12	23.85765101347904	26.734664672455676	26.557866637039307	22.849817677025978
13	22.927227199363333	26.470312965128883	25.266721694652645	25.335738140855142
14	19.887846765374878	25.911056338070694	27.209145192367494	26.99195170418694
15	22.61849483765436	30.654170845593363	25.53276409357495	21.19457022317733
16	22.696447796575985	29.484574552555447	24.90286071056145	22.916116940307113
17	23.922621876220845	30.316153290176416	24.03438866705271	21.72683616655003
18	23.57747926336567	26.503643742297538	24.130576942468785	25.788300051868003
19	25.574306801350378	25.019276903280968	21.547683239268487	27.858733056100167
20	22.571397000350817	27.485754413761747	24.661635248987547	25.28121333689989
21	24.57649685078499	29.89625795606255	24.844350704988205	20.68289448816425
22	26.087975137172453	25.979710493217006	23.27846837833089	24.653845991279656
23	24.89899627262885	24.908053549033376	28.585307769271317	21.607642409066454
24	27.788267445672947	27.324595275603862	24.817843076044287	20.0692942026789
25	27.334171203436647	26.00897926941953	25.112027540001996	21.544821987141823
26	23.88604879490076	30.69037367155929	22.528323140798555	22.895254392741396
27	27.145271498711175	29.218953661327586	23.03513922237851	20.60063561758273
28	26.83328982174929	27.41884041104293	21.22765575312261	24.52021401408517
29	26.391752840497517	27.907429425531195	24.868673060219248	20.832144673752033
30	29.637158129584627	26.58520810571048	22.828857549174614	20.94877621553028
31	26.066232635921654	27.756691234279312	25.566838718838003	20.61023741096103
32	24.413167048557515	25.521784621645093	28.668762855550938	21.396285474246454
33	25.027244057218024	25.7871881381026	31.2451497322377	17.940418072441673
34	24.2325903548182	24.372292365378534	29.25598127342638	22.139136006376887
35	21.743441096141673	26.449885812785773	27.087555573774253	24.719117517298304
36	23.68428583893203	26.079050693656747	27.28627519413664	22.950388273274584
37	25.711555515642715	28.361612204348752	24.837019970140204	21.089812309868325
38	24.659079452835122	29.194487743216314	23.977696608736785	22.16873619521178
39	23.584714792423895	29.240832992596218	22.747279644482678	24.427172570497213
40	21.320741446447183	27.40269954027251	25.938761257853056	25.33779775542725
41	22.697618656828254	27.77650912360577	24.055490933483668	25.47038128608231
42	23.101900932319005	26.37943475560159	25.58512019441125	24.933544117668156
43	23.492423290238044	24.93553042747119	28.315614133174993	23.256432149115774
44	26.039453606700746	24.29657168576165	26.76024487436101	22.903729833176598
45	23.433195920825362	24.225385008170715	28.99340313105123	23.348015939952692
46	23.832002008186574	26.77024266432942	26.129414777582348	23.268340549901662
47	22.313815530945572	30.157390884244546	23.969746769326957	23.55904681548293
48	25.132110357206788	26.80767429371535	26.722829405374654	21.33738594370321
49	21.999423537677217	29.761488713948587	22.56976094828052	25.66932680009367
50	18.16665857172924	33.44990043226966	31.199912574675796	17.183528421325303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65774.0
1	66043.5
2	47021.5
3	27353.0
4	25715.0
5	17244.5
6	6653.0
7	3381.5
8	3192.0
9	2610.0
10	2556.5
11	3233.5
12	3647.0
13	3944.5
14	4676.0
15	5125.5
16	5199.5
17	5481.5
18	6141.0
19	7518.0
20	8877.0
21	9405.5
22	9358.5
23	9991.5
24	11862.5
25	13481.5
26	17110.0
27	24081.0
28	27271.0
29	22677.0
30	18430.0
31	24039.0
32	37635.0
33	46181.0
34	46663.0
35	44976.5
36	45264.0
37	47968.0
38	50688.5
39	54711.5
40	59365.5
41	70635.0
42	83980.0
43	85965.0
44	85151.0
45	86023.5
46	89840.5
47	100983.0
48	118893.5
49	139703.5
50	155365.5
51	155909.5
52	160996.0
53	172949.5
54	167660.0
55	156548.5
56	146405.0
57	139768.5
58	130746.5
59	108912.0
60	87597.0
61	71088.0
62	56957.5
63	48367.0
64	41787.0
65	34145.5
66	22878.5
67	13880.5
68	9749.0
69	7084.5
70	5668.5
71	4030.5
72	2816.0
73	1832.0
74	929.5
75	613.5
76	436.5
77	260.5
78	142.0
79	117.5
80	101.0
81	72.5
82	33.5
83	10.5
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15459.0
25	16113.0
26	15726.0
27	19214.0
28	22724.0
29	19192.0
30	26455.0
31	36963.0
32	30753.0
33	8309.0
34	5916.0
35	6678.0
36	7297.0
37	6889.0
38	4837.0
39	6029.0
40	6263.0
41	7152.0
42	9784.0
43	17959.0
44	56248.0
45	127010.0
46	162704.0
47	204847.0
48	260496.0
49	308042.0
50	247068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.3579656701567435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.05260696192222	5.007287457904935
2	11.770424962539034	1.7321276559503636
3	4.908223022100964	1.0834360949427562
4	2.7608438660132038	0.812567775471519
5	1.7506876685016646	0.6440749882000998
6	1.1551045191537899	0.5099531638425899
7	0.8489169065588357	0.4372411018692901
8	0.6454249163220975	0.37992115015694256
9	0.49949211455632603	0.3307721248277502
>10	4.40119210429914	6.740902472523101
>50	0.8722331705934054	4.573186651218598
>100	1.6669159732468617	30.651118581643022
>500	0.505328203995797	25.918835224358688
>1k	0.15343298603172714	16.401195370281005
>5k	0.008338749240854737	4.153694102290154
>10k+	8.338749240854736E-4	0.6236860845191795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10165	0.6137814310134427	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	8484	0.5122795534400442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	8163	0.49289698193435644	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	7886	0.47617121150733005	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6929	0.4183857880464481	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	6838	0.41289104036103513	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	6408	0.38692684800139115	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6073	0.3666989306979477	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6039	0.3646459480462549	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	5623	0.3395271014843668	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	5255	0.3173065833719274	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	4524	0.27316745636053275	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3867	0.2334965857087047	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	3564	0.2152008873715603	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3490	0.21073263101199363	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	3420	0.2065059020232144	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	3171	0.19147082319169967	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA	3095	0.18688180314673936	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCCC	3056	0.18452691128156234	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3032	0.18307774705683802	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2927	0.17673765357366916	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	2893	0.1746846709219764	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC	2537	0.1531887349218991	No Hit
CACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAAGGCCTTCAC	2491	0.15041117015784417	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2399	0.1448560406297343	No Hit
AGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTTGTGCCAGC	2248	0.13573838238251051	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2222	0.1341684544723925	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	2214	0.13368539973081775	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	2194	0.13247776287688082	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2176	0.1313908897083376	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAACA	2131	0.1286737067869795	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2110	0.12740568809034575	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2018	0.12185055856223587	No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA	2004	0.12100521276448002	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	1949	0.11768421141615348	No Hit
TCTGCACTTCGGCTGCTCTCGGGTTAGCACCCTATGGTGCCTTCTCTT	1943	0.1173219203599724	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1927	0.11635581087682284	No Hit
CCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA	1923	0.11611428350603546	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	1834	0.11074029950601613	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1831	0.1105591539779256	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	1819	0.10983457186556345	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT	1816	0.10965342633747291	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	1810	0.10929113528129185	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	1803	0.10886846238241392	No Hit
CCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGC	1771	0.10693624341611484	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC	1769	0.10681547973072113	No Hit
TCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGG	1751	0.1057286065621779	No Hit
ACCCAGCGAACAATGAACCCTCTCTACTCATTTTCCTGTTCCTCTAAAT	1728	0.10433982418015045	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1685	0.10174340494418603	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1682	0.10156225941609551	No Hit
CCATCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGTCGG	1669	0.1007772954610365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08362885213513215	0.0	0.0	0.0	0.0
2	0.13887823820274653	0.0	0.0	0.0	0.0
3	0.18935745869731005	0.0	0.0	0.0	0.0
4	0.2475655550570699	0.0	0.0	0.0	0.0
5	0.29351613734936993	0.0	0.0	0.0	0.0
6	0.4120456945632793	0.0	0.0	0.0	0.0
7	0.4614984237319964	0.0	0.0	0.0	0.0
8	0.517834682968154	0.0	0.0	0.0	0.0
9	0.5791826351481498	0.0	0.0	0.0	0.0
10	0.6227783255752729	0.0	0.0	0.0	0.0
11	0.6749482376653482	0.0	0.0	0.0	0.0
12	0.7230725662947346	0.0	0.0	0.0	0.0
13	0.7728875865196329	0.0	0.0	0.0	0.0
14	0.8137660940253978	0.0	0.0	0.0	0.0
15	0.8585694213064577	0.0	0.0	0.0	0.0
16	0.8991460195987385	0.0	0.0	0.0	0.0
17	0.9507724951045421	0.0	0.0	0.0	0.0
18	0.9848882362282603	0.0	0.0	0.0	0.0
19	1.0217815421160334	0.0	0.0	0.0	0.0
20	1.0645318867454006	0.0	0.0	0.0	0.0
21	1.095809681262367	0.0	0.0	0.0	0.0
22	1.1219550191501013	0.0	0.0	0.0	0.0
23	1.1534139591951584	0.0	0.0	0.0	0.0
24	1.1889788645436008	0.0	0.0	0.0	0.0
25	1.2180829127234807	0.0	0.0	0.0	0.0
26	1.248273834071904	0.0	0.0	0.0	0.0
27	1.275385281442788	0.0	0.0	0.0	0.0
28	1.3027986380271561	0.0	0.0	0.0	0.0
29	1.3323253591059139	0.0	0.0	0.0	0.0
30	1.3592556609487074	0.0	0.0	0.0	0.0
31	1.3873332178027409	0.0	0.0	0.0	0.0
32	1.4145654288590186	0.0	0.0	0.0	0.0
33	1.4456620778478946	0.0	0.0	0.0	0.0
34	1.4808646921401558	0.0	0.0	0.0	0.0
35	1.5078553758256463	0.0	0.0	0.0	0.0
36	1.5289890207695425	0.0	0.0	0.0	0.0
37	1.5435410448594824	0.0	0.0	0.0	0.0
38	1.5559797044550328	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACA	45	0.0	261.8122	44
CGTGACT	15	1.1647371E-5	261.8122	44
GCTATGT	10	0.0012095904	261.8122	44
TGTATAC	25	1.0822987E-9	261.8122	44
GATAGTA	15	1.1647371E-5	261.8122	44
GATGTCA	65	0.0	261.81216	44
GCACGGG	115	0.0	261.81216	44
GGTCAAT	85	0.0	261.81216	44
TGTTAAC	65	0.0	261.81216	44
CTCATGA	165	0.0	245.94478	44
TCGTGAC	75	0.0	244.35803	44
TACTAGT	75	0.0	244.35803	44
ACGAACA	155	0.0	236.47552	44
ACATAGC	90	0.0	232.72194	44
GTAAAGG	90	0.0	232.72194	44
ACCTGGA	115	0.0	227.66277	44
CCATTAC	25	3.406858E-7	209.44975	44
TGTACCA	145	0.0	207.64415	44
TAAATCT	160	0.0	204.54077	44
CTATCAT	835	0.0	202.23817	44
>>END_MODULE