FastQCFastQC Report
Tue 24 May 2016
ERR841093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692009
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC104030.6148312449874676No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT82860.4897137071965929No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA58450.34544733509100717No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC47930.28327272490867367No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC43760.2586274659295547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA39640.23427771365282335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA37970.22440778979308026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA37220.21997518925726753No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT36240.2141832578904722No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.20892323858797443No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32700.19326138336143603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31500.18616922250413562No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT31320.18510539837554055No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG31300.18498719569458555No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT30740.18167752062784537No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA30550.18055459515877278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA30190.17842694690158267No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG28460.1682024149989746No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA26540.15685495762729396No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT25520.1508266208985886No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT24750.14627581768182085No Hit
AAAAAAAAAAAAAAAAAAAAAAAA24410.1442663721055857No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.1424342305507831No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCG23810.14072029167693553No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC21540.12730428738854227No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT20460.12092134261697189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20060.11855728899787177No Hit
CTCCTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTC20000.11820268095500674No Hit
ACAACCTTAGCTAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT19800.11702065414545668No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC19200.11347457371680648No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC19040.11252895226916643No Hit
AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA18990.11223344556677892No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC18690.1104604053524538No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG18530.10951478390481374No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG17910.10585050079520854No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC16930.10005856942841322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGC1050.0155.9819244
GAGCTTA550.0155.981944
CTGTAGC1100.0155.981944
TTTCGTA400.0155.981944
CGCATTG100.005699166155.981944
CCCGTAC850.0155.981944
GTCCCCG252.39761E-8155.981944
ACATATA750.0155.981944
AGGTGTG650.0155.981944
GTAACTC900.0155.981944
CGTTATA600.0155.9818944
TCTACGC600.0155.9818944
AATTGTC1200.0155.9818944
CGATCTA1200.0155.9818944
GCTCCCC1200.0155.9818944
ACGAACA1650.0151.2551744
GAGTACC1100.0148.8918244
TACTGGG1050.0148.554244
GGGACCC1000.0148.182844
CGTTTGA1600.0146.2330344
Produced by FastQC (version 0.11.5)