##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841093.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1692009
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.47338518884947	39.0	37.0	39.0	35.0	39.0
2	37.91984321596398	39.0	38.0	39.0	35.0	39.0
3	38.072880818009835	39.0	38.0	39.0	35.0	39.0
4	38.40729452384709	39.0	38.0	40.0	35.0	41.0
5	39.24720613188228	40.0	39.0	41.0	36.0	41.0
6	39.19904090344673	40.0	39.0	41.0	35.0	41.0
7	39.241895876440374	40.0	39.0	41.0	36.0	41.0
8	39.17099731739015	40.0	38.0	41.0	35.0	41.0
9	39.090320441557935	40.0	38.0	41.0	35.0	41.0
10	39.024014647676225	40.0	38.0	41.0	35.0	41.0
11	38.90819138668884	40.0	38.0	41.0	35.0	41.0
12	38.84002390058209	40.0	38.0	41.0	35.0	41.0
13	38.68927352041272	40.0	38.0	41.0	35.0	41.0
14	38.63752615973083	40.0	38.0	41.0	35.0	41.0
15	38.4844548699209	40.0	38.0	41.0	35.0	41.0
16	38.51305223553776	40.0	38.0	41.0	35.0	41.0
17	38.65764366501597	40.0	38.0	41.0	35.0	41.0
18	38.622069977169154	40.0	38.0	41.0	35.0	41.0
19	38.502276879141895	40.0	38.0	41.0	35.0	41.0
20	38.468294199380736	40.0	37.0	41.0	35.0	41.0
21	38.501054663420824	40.0	37.0	41.0	35.0	41.0
22	38.46523629602443	40.0	37.0	41.0	35.0	41.0
23	38.43453492268658	40.0	37.0	41.0	35.0	41.0
24	38.29115034258092	40.0	37.0	41.0	34.0	41.0
25	38.16432466112513	40.0	36.0	41.0	34.0	41.0
26	38.10854250784367	40.0	36.0	41.0	34.0	41.0
27	37.98610528017449	40.0	36.0	41.0	34.0	41.0
28	37.98063274909006	39.0	36.0	41.0	34.0	41.0
29	37.918544074775824	39.0	36.0	41.0	34.0	41.0
30	37.95989942433338	39.0	36.0	41.0	34.0	41.0
31	37.8275166811447	39.0	35.0	41.0	34.0	41.0
32	37.71109346055367	39.0	35.0	41.0	33.0	41.0
33	37.4822452990893	39.0	35.0	41.0	33.0	41.0
34	37.41299942286863	39.0	35.0	41.0	33.0	41.0
35	37.37768952409189	39.0	35.0	41.0	33.0	41.0
36	37.34509038629114	39.0	35.0	40.0	34.0	41.0
37	37.20845168984384	38.0	35.0	40.0	33.0	41.0
38	37.11679886385144	37.0	35.0	40.0	33.0	41.0
39	36.94641951940292	37.0	35.0	40.0	33.0	41.0
40	36.86393226480101	37.0	35.0	40.0	33.0	41.0
41	36.799855625283094	37.0	35.0	40.0	33.0	41.0
42	36.674967607693326	37.0	35.0	39.0	33.0	41.0
43	36.48493199804639	36.0	35.0	39.0	33.0	41.0
44	36.25380697292465	36.0	35.0	39.0	33.0	41.0
45	36.05530947613093	36.0	35.0	39.0	33.0	41.0
46	35.73227297232493	36.0	35.0	39.0	32.0	40.0
47	35.46897436219839	35.0	34.0	38.0	31.0	40.0
48	35.29020857293836	35.0	34.0	37.0	31.0	39.0
49	35.426391888667496	35.0	34.0	37.0	32.0	39.0
50	34.98463964666994	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	16.0
22	52.0
23	87.0
24	161.0
25	297.0
26	658.0
27	1350.0
28	2836.0
29	5553.0
30	10623.0
31	18618.0
32	30830.0
33	51740.0
34	84522.0
35	108856.0
36	184096.0
37	253386.0
38	286384.0
39	386960.0
40	264981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.09624121384697	17.456231024775875	31.447527761377152
2	22.147754533220567	23.454248765816256	33.745624284504395	20.65237241645878
3	22.64125072620772	22.347635266715486	29.543105267170567	25.468008739906228
4	18.256581377522224	25.127466816074858	34.49703872733538	22.118913079067546
5	15.675566737529174	26.695070770900152	33.24072153280508	24.38864095876559
6	17.200322220508284	30.748536207549726	28.894704460791875	23.15643711115012
7	23.032501600168793	28.650143113895965	23.7969774392453	24.520377846689943
8	21.298409169218367	27.75192094131887	29.066571158900455	21.88309873056231
9	20.788128195535602	28.75676193211738	27.858303354178375	22.596806518168638
10	21.0306209955148	25.46168489647514	29.254336117597486	24.25335799041258
11	22.783980463460892	24.63828502094256	28.63968217663145	23.9380523389651
12	20.436416118353982	26.225687924827824	30.703264580743955	22.63463137607424
13	24.14088813948389	23.99815840223072	28.18217869999509	23.678774758290295
14	19.54664543746517	26.971605942994394	28.033066017970356	25.448682601570088
15	20.930266919384	28.651857052769813	29.616036321319804	20.801839706526383
16	17.827387442974594	32.79681136447856	26.75842740789204	22.61737378465481
17	19.65456448517709	34.49402455897102	25.18674546057379	20.664665495278097
18	21.82547492359674	28.624256726766824	23.397452377617377	26.152815972019063
19	19.60509666319742	26.685614556423754	27.23360218533116	26.475686595047666
20	19.94215160794062	26.17551088676242	30.781573856876648	23.100763648420312
21	22.04166762706345	28.737258489759803	26.820011004669595	22.401062878507148
22	22.000060283367286	27.734545147218487	27.0680002293132	23.197394340101027
23	24.31825126225688	26.594362086726488	28.52916266993852	20.558223981078115
24	22.95732469508141	27.642287954733103	29.262314798561945	20.138072551623544
25	25.713883123469422	25.96073754297806	27.35862798408764	20.966751349464882
26	25.01762656110282	30.838880662495345	26.259266663235124	17.88422611316671
27	21.545773254579498	29.873738104889302	27.074526608373382	21.505962032157814
28	22.895945656616774	27.92634593300504	24.579456760807364	24.59825164957082
29	18.894678665356796	29.15901789106504	29.55898789024125	22.387315553336915
30	23.153377271065942	29.046870569020733	27.333336229144727	20.466415930768598
31	22.99187157324409	30.35186333238488	24.128946834523923	22.527318259847103
32	23.271940491351476	28.076250408160263	26.243538926827647	22.40827017366061
33	22.34816222236782	26.821005044879776	28.62189608858023	22.20893664417218
34	21.78012085499772	28.54052964320632	29.755667878027637	19.923681623768324
35	20.095258390203355	29.35909382097723	28.52172796628565	22.023919822533763
36	22.870652920231283	25.777490932125037	29.527539952156452	21.824316195487235
37	19.84529852550157	32.87620009161362	27.566683627204704	19.711817755680112
38	25.349375078022963	27.78791983065158	24.488202928159815	22.374502163165644
39	22.091179706205917	28.088194848896652	26.630009814706963	23.19061563019047
40	20.097657531624034	28.21873771754947	28.04833087205145	23.635273878775045
41	20.31439190469367	31.984907884720254	25.632352431858322	22.06834777872775
42	21.105448051478167	31.05196831902702	25.992214923808106	21.85036870568671
43	22.10360586549908	25.81759306262609	26.948131122631576	25.130669949243252
44	21.79333476552484	27.10902904345777	26.864470793688195	24.23316539732919
45	19.97245492948519	27.669265455291246	30.843215281674695	21.51506433354887
46	23.762441258626982	27.455759747211644	26.001218735538973	22.7805802586224
47	20.904966077917507	32.64180903403345	27.156526565562032	19.296698322487007
48	21.207816563571747	28.526830582408586	25.71384165863666	24.551511195383004
49	20.025626803095047	28.490063803612543	24.232048068073713	27.252261325218697
50	21.3132473147335	27.464609922965288	28.719947261389233	22.502195500911977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35541.0
1	35921.0
2	35992.0
3	35517.5
4	33837.0
5	23610.0
6	11170.0
7	8233.5
8	9395.0
9	9182.0
10	8480.5
11	7348.5
12	5879.0
13	5208.0
14	5197.0
15	5269.0
16	4872.5
17	5073.0
18	6442.5
19	8494.0
20	11000.5
21	13227.5
22	14205.5
23	14222.0
24	13483.5
25	14771.0
26	19780.5
27	26730.5
28	31841.0
29	36039.0
30	40351.0
31	47909.5
32	60881.5
33	70561.0
34	79084.5
35	85759.5
36	83166.0
37	80865.0
38	86900.0
39	94889.0
40	100117.5
41	103737.0
42	106296.0
43	105294.0
44	107583.0
45	121938.0
46	135736.5
47	132509.5
48	128249.0
49	133337.5
50	139984.5
51	142752.0
52	134769.5
53	117567.0
54	106774.5
55	103727.0
56	104475.5
57	108438.0
58	105938.5
59	85632.5
60	59304.0
61	41873.5
62	27760.5
63	18727.5
64	13397.0
65	10323.0
66	7595.5
67	4723.0
68	2898.0
69	1848.5
70	1181.5
71	831.0
72	483.5
73	319.0
74	241.0
75	186.5
76	143.5
77	76.5
78	21.0
79	12.5
80	7.0
81	6.0
82	5.5
83	6.5
84	6.5
85	4.5
86	4.5
87	3.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10046.0
25	11189.0
26	12950.0
27	13760.0
28	17442.0
29	15099.0
30	19456.0
31	27118.0
32	38649.0
33	8448.0
34	6804.0
35	6561.0
36	6893.0
37	7648.0
38	6458.0
39	5261.0
40	5670.0
41	7703.0
42	9087.0
43	12757.0
44	38766.0
45	94690.0
46	196120.0
47	148304.0
48	319712.0
49	216131.0
50	429287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.505865514313922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.73723345620529	5.159324301482766
2	12.016852850608027	1.803937628039275
3	5.046190320599732	1.1362807771816272
4	2.6900059076672918	0.807632903026426
5	1.5558430626662179	0.583897439487546
6	1.0796339105790027	0.4862152162499291
7	0.7759266309951459	0.40768006588569755
8	0.5723123199734537	0.3436559444724593
9	0.4262299292317891	0.28793020742904146
>10	3.817674303736744	6.131846672471528
>50	0.8125425727812682	4.351441086425705
>100	1.7966928598532759	34.356452534665735
>500	0.5451538086538371	28.507441847130572
>1k	0.125313540203005	14.166432092552906
>5k	0.001596350830611529	0.8465878318709352
>10k+	7.981754153057645E-4	0.6232434516278635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10403	0.6148312449874676	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8286	0.4897137071965929	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5845	0.34544733509100717	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4793	0.28327272490867367	No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC	4376	0.2586274659295547	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	3964	0.23427771365282335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3797	0.22440778979308026	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3722	0.21997518925726753	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3624	0.2141832578904722	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3535	0.20892323858797443	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3270	0.19326138336143603	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3150	0.18616922250413562	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	3132	0.18510539837554055	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	3130	0.18498719569458555	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	3074	0.18167752062784537	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3055	0.18055459515877278	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3019	0.17842694690158267	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	2846	0.1682024149989746	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2654	0.15685495762729396	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	2552	0.1508266208985886	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT	2475	0.14627581768182085	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2441	0.1442663721055857	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2410	0.1424342305507831	No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCG	2381	0.14072029167693553	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	2154	0.12730428738854227	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	2046	0.12092134261697189	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2006	0.11855728899787177	No Hit
CTCCTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTC	2000	0.11820268095500674	No Hit
ACAACCTTAGCTAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1980	0.11702065414545668	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	1920	0.11347457371680648	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1904	0.11252895226916643	No Hit
AGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGA	1899	0.11223344556677892	No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC	1869	0.1104604053524538	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	1853	0.10951478390481374	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1791	0.10585050079520854	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1693	0.10005856942841322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.037706655224647154	0.0	0.0	0.0	0.0
2	0.0718672300206441	0.0	0.0	0.0	0.0
3	0.10484577800709098	0.0	0.0	0.0	0.0
4	0.14621671634134334	0.0	0.0	0.0	0.0
5	0.17624019730391505	0.0	0.0	0.0	0.0
6	0.24982136619840675	0.0	0.0	0.0	0.0
7	0.2885327442111715	0.0	0.0	0.0	0.0
8	0.3367594380408142	0.0	0.0	0.0	0.0
9	0.38776389487289964	0.0	0.0	0.0	0.0
10	0.42913483320715196	0.0	0.0	0.0	0.0
11	0.473756345267667	0.0	0.0	0.0	0.0
12	0.5116403045137466	0.0	0.0	0.0	0.0
13	0.5499379731431688	0.0	0.0	0.0	0.0
14	0.5839212439177333	0.0	0.0	0.0	0.0
15	0.6271243238067883	0.0	0.0	0.0	0.0
16	0.6648309790314354	0.0	0.0	0.0	0.0
17	0.7177857800992784	0.0	0.0	0.0	0.0
18	0.7472182476570751	0.0	0.0	0.0	0.0
19	0.7801376943030445	0.0	0.0	0.0	0.0
20	0.8131162422894914	0.0	0.0	0.0	0.0
21	0.851000201535571	0.0	0.0	0.0	0.0
22	0.8781277168147451	0.0	0.0	0.0	0.0
23	0.9052552320939191	0.0	0.0	0.0	0.0
24	0.9361061318231759	0.0	0.0	0.0	0.0
25	0.9648293832952425	0.0	0.0	0.0	0.0
26	0.9960939924078418	0.0	0.0	0.0	0.0
27	1.023339710367971	0.0	0.0	0.0	0.0
28	1.0535404953519751	0.0	0.0	0.0	0.0
29	1.0825001521859516	0.0	0.0	0.0	0.0
30	1.1151831934700112	0.0	0.0	0.0	0.0
31	1.1451475730921052	0.0	0.0	0.0	0.0
32	1.1728070004355768	0.0	0.0	0.0	0.0
33	1.2029486840791035	0.0	0.0	0.0	0.0
34	1.2324993543178553	0.0	0.0	0.0	0.0
35	1.2602178830018043	0.0	0.0	0.0	0.0
36	1.2825581897023006	0.0	0.0	0.0	0.0
37	1.3026526454646519	0.0	0.0	0.0	0.0
38	1.3153594336673151	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTCGC	105	0.0	155.98192	44
GAGCTTA	55	0.0	155.9819	44
CTGTAGC	110	0.0	155.9819	44
TTTCGTA	40	0.0	155.9819	44
CGCATTG	10	0.005699166	155.9819	44
CCCGTAC	85	0.0	155.9819	44
GTCCCCG	25	2.39761E-8	155.9819	44
ACATATA	75	0.0	155.9819	44
AGGTGTG	65	0.0	155.9819	44
GTAACTC	90	0.0	155.9819	44
CGTTATA	60	0.0	155.98189	44
TCTACGC	60	0.0	155.98189	44
AATTGTC	120	0.0	155.98189	44
CGATCTA	120	0.0	155.98189	44
GCTCCCC	120	0.0	155.98189	44
ACGAACA	165	0.0	151.25517	44
GAGTACC	110	0.0	148.89182	44
TACTGGG	105	0.0	148.5542	44
GGGACCC	100	0.0	148.1828	44
CGTTTGA	160	0.0	146.23303	44
>>END_MODULE