FastQCFastQC Report
Tue 24 May 2016
ERR841092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893418
Sequences flagged as poor quality0
Sequence length24-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA84210.9425599215596732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA80890.9053992643980758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA79740.8925273500198114No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC68220.7635843468566785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67840.7593310186273391No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA61380.6870244387285683No Hit
AAAAAAAAAAAAAAAAAAAAAAAA50120.5609916075118254No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT40670.45521805022956774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39840.44592788593916843No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA39740.4448085890367107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23060.25810986570675765No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT21470.2403130449576794No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC21060.2357239276576026No Hit
CATGAAATGCAGTCCCTGGATTCTGTGCCATCTGAACATGCTCTCCTGTT18710.20942045044984545No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA17950.20091379399116652No Hit
CATACTCAAAAACTTATGTGTGGAGCTGTATCCTGCCCCCCGAGAGAGTT16650.1863629342592157No Hit
ATGCCTGGAGCTGTATCCTGCCCCGCAGGAAAGTTATGGTGCTGATGGTA16190.18121416850791006No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG16100.1802068012956981No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA15690.1756176839956213No Hit
ATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGAC15630.17494610585414666No Hit
AGCCTACACCCAGGAATGAACAAGGACAGCTTGGAGGTTAGAAGCAAGA15310.17136435576628184No Hit
TGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCTAATTA14430.16151454302465365No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC14220.15916401952949236No Hit
ATAGTGAAATCCCATCTCTACTAAAAAATACAAAATGGTGGTGTATGGAC13990.15658963665383954No Hit
AGAGATGCATGCCTTCTGTCTTTATTATGTTCCTCTGGTTTTGGGAGT13650.15278402718548317No Hit
ACCAATGAGATGGCTTAGGGTGGAGACCCTAGACAGCCTCAAGATGAAGC13620.15244823811474584No Hit
AAGGCAAGATTGGTTTTGAAATGTGAGGACATTAGATTTGGGAATGGCC13190.14763526143417752No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGAC13180.14752333174393173No Hit
AGCAGCAGTTGTTTCAGCTGGTGCCCATCCTTGAGGCTGTCCTCTGTAGG13170.14741140205368597No Hit
AGAGATGAATGTCTGATGTGTGGATCCTGAGGAAAGACTTGGAAGAGACC13000.14550859731950777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.1434938628950838No Hit
TGTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGG12700.14215070661213453No Hit
AGTGCCAGCCTAGGCTCTCATTAACCTGCCAAATCATCTCATCTCTCT12680.14192684723164298No Hit
CTTCCTCTCCACTCCAGACGCTGGCTCTTGAGTTGAAGAAACTTTATTT12680.14192684723164298No Hit
CATGATTGTAGGTTTCCTGAGGCCTCCCCAGCCATGCAGAACTGTGTGTT12620.1412552690901683No Hit
AGAAAAATCAAGAAAGATGGGAAGGAAGGTGCTGAGGAAATAGACAGACA12570.14069562063893945No Hit
TTCTTTAGATGGTGGCACATTCCTTTGGGTATGTTAACCTAGGCAGTGGG12420.1390166752852528No Hit
CGGAAACCATGCAATGAAACCAATAAATGATAATAAAGAGCCTCTGAACT12390.1386808862145155No Hit
AATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAG12300.1376735190023035No Hit
AATGACCTCCGACACATCAGCCCTCAAGCCAAAGCTCTCCTTCAGGATAA12260.13722580024132042No Hit
CTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGG12250.13711387055107466No Hit
CAGCTTTTGGATTTGTGACTTATACAAATGTCAAAGTAGCAGGTATATTA12240.13700194086082887No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA12100.135434925197388No Hit
CTGCTTCAGCCTCCTGAGTAGCTGGGACTACAGATATATACTACCATGCC11910.1333082610827183No Hit
ACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCT11830.1324128235607521No Hit
AGAGCTCACATTAACGGTGGGAGACACAGATCTTGAGCAAGAGTAGATAA11630.1301742297558366No Hit
AGTTCAGCGCAGCGACTCGGGGACCTGGAGCTGACGCCTAGACACTTGT11620.1300623000655908No Hit
ATCGACGCAGCGTCCCCACTTGGTTGAAGTTGACATCTGACGACGTGAAG11590.12972651099485347No Hit
AGGATCTTTGTTGCTGAACATTTTGTGGCCCCTCAAATCTCACATCAGAA11530.12905493285337882No Hit
AAGCTGATGGGCTGAGAACATGGACTGAGCTTGTGGTGTGCTTTGCAGG11470.12838335471190418No Hit
AGCATCTGTAATCATTGAGAATGAAGGAAAACTTGAAAGAGAGAAAAAAA11400.12759984688018375No Hit
AGTGGAAGGCAAGGATTGGCATCTCTAATATAATTATACAGGTAATGAT11340.12692826873870908No Hit
AGCCAAAATGTACAAGACCACACCGGATGTCATCTTTGTATTTGGATTCA11310.12659247966797177No Hit
TGTGGGGAGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATT11290.12636862028748022No Hit
ATCACTGTACAGTTTATAAATCAATGGATGAATGATACAGATTGTTTTCT11190.12524932338502248No Hit
AGTGCAGTGCTAAAACGGGGATTGTTCAGGATGTGAGCGTGGCAAATTT11140.12468967493379358No Hit
TACGTGGACACTATAAAGGTCAGCAAATTGGCAAAGTAGTCCAGGTTTA11120.12446581555330204No Hit
ATTTACGGGAGGCTGTTCGCAGGTTAAAGCCCAAGGGCCTCCTCCCCAAC11110.12435388586305626No Hit
CTCTGAAACATGTGCTATGTCCACTCAGGGTTAAATGGATTAAGGGCGGT11100.12424195617281047No Hit
CCTGGGAGGAAGCCCAAAGTCCTGCCAACTGCCGTGCATGCAGGGAACCA11100.12424195617281047No Hit
ATTCTTATTTTGATGTGTGTGAAAATTGATCTCGTCAATGGCTAGGGGTA11100.12424195617281047No Hit
AACATCATTGATTATCAGAGAAATGCAAATCAAAACTACAGTGAAATATC11090.12413002648256471No Hit
AATGCCTGCCTATTTGGATGGGTATGTCAAACGCTTTCTTCTGGACACT10980.12289879988986117No Hit
TCTCCAGACTGAATGAAGCCAGTCAGCTGGGATTCTGGTTGAGTCTCA10950.12256301081912387No Hit
CTGGTGGAATTATCAGGAATTATTGATTCAGACTTCCTCTCAAAATGTGA10870.12166757329715766No Hit
ATGTGTATGAGCAAAAATCTTGAGGACTGTATTTGTGACTAATTGTATAA10790.12077213577519146No Hit
AGGGGAGAGCTTGCTGCAGATTCTGACAGTGCAGATTTTAAATGTCAGGA10690.1196528388727337No Hit
AGTCATAGAATTCTCTCTGGAGGAGTGGCAATGCCTGGATCACGCTCAGC10640.11909319042150482No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA10540.11797389351904707No Hit
CACGTGAAAGATGCCAATGGCAACAGCTTTGCCACTCGACTTTCCAACAT10510.11763810444830976No Hit
ATTTTGGGGCTCTAATAAATGTCTGTGAAATCTTCACAATTTATTTCTTC10500.11752617475806397No Hit
CATGAAATGCAGTCCCTGGATTCTGTGCCATCTGAACATGCTCTCCTG10350.11584722940437735No Hit
CAGAATAAGTATTTTGGTGATGTAGATATTCCTCGAGCCAAAGTGGTGAG10330.1156233700238858No Hit
AGCACGGACTGCGGGAGGTAGTGGCCTTGCCAGGGCTGCCTCAAAGGATA10290.1151756512629027No Hit
ATGCAGGAAATGCTCTATGTACAGTGAAGTGTGATGAGAAAGTGAAAGTG10260.11483986219216537No Hit
ATCATTGGCTAATAATAGACCTCTGTTAAATCAATGAAAGGAGGAAGGAC10250.1147279325019196No Hit
ATCTTTCGCCTGTGCTGGATGCTTCCTGCCCTTGAACATCTGACTCCAAG10240.1146160028116738No Hit
AAACACAAGGTGCTGGTCTACCTTCCGTGCCACTGGAATCTCTGAACAAA10190.11405635436044495No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG10180.11394442467019916No Hit
AGTCCATCTAGCAACTCACTCCAGACTTTATGAAAATCTTTTTCGTGGAC10150.11360863559946184No Hit
AGTCCTGCAGGGGATATTTGGCAATGTCTGGGGACTTTGATTGTCTTAAG10100.11304898714823296No Hit
AAAAGAGATGTCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGA10070.11271319807749565No Hit
ATGTAGTAAGAACCTGGGATATTCCTGACCCAATCTGGTTTTCTTTTAC10050.11248933869700409No Hit
AGAGGAACCCTGCGGAGGGACTTCAATCACATCAATGTAGAACTCAGCCT9980.11170583086528366No Hit
CTCTCAGGAGGGTATGTATTGGGTGGAGGAGCATTATGTATGGAACTTCT9980.11170583086528366No Hit
TGTGAAGCCCAGAAATGGCATTTGCAGTTAATTAGCACATGTGAGGGTT9970.1115939011750379No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA9910.11092232303356324No Hit
TGGTATCAACGCAGGCAGTGCTAAAACGGGGAGTCCAAAGAGGAACAGC9900.11081039334331745No Hit
TTGGTTTCAGTGCTGATGAAACTTGGGACTCTGCAATAGATACATGGA9860.11036267458233436No Hit
ATTGAGGAAATTTCCTAACAAACAAACAAACAAACAAACAGAAGAGAAGA9840.11013881520184281No Hit
AAGATTTGTTCAAAAATGTTATAAAACCTGAAACAATTGGTGCCACTAT9830.11002688551159703No Hit
ATTGCTGTTCCCACAAAGGACTAAGCCTGTTCATAAGTTACCAAAGTTGC9830.11002688551159703No Hit
ACAGTATATTTTATAGATTCTCTCTCTTTCTCTCTTAAAAACAAGACACA9770.10935530737012239No Hit
CGAAACGCCAGCGCATATCAGGAAACACCTCACGAAGGGGCAAAAGTGGC9720.1087956589188935No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA9640.1079002213969273No Hit
TACCCCTGAACCTAAAATAAAAATTAAAATAAACGGAAAGGGGTTCAAC9540.10678092449446955No Hit
AGGAAAAAGACCCAAACAAATCTAACTTCTTGCTTTCTCACCTATTTGAA9540.10678092449446955No Hit
CTTGTTCGCTGGAAATCCAGGGAGGTGTTTGGTGAATTCATGGCTGCACC9540.10678092449446955No Hit
TACCAATCAGCAGGAACCTAGACTCCTGAGGGGTGCAGCCTGGATGGGAG9520.106557065113978No Hit
AACCATTGTGGAAAACAGTGTGGTGATTCCTCAAGGATCTTGAACTAGAA9510.10644513542373223No Hit
AGAGACAAGCCTAGCCTCTCAGCCTACATCTTTCTCCCATGCTGGATG9480.10610934635299489No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA9470.10599741666274913No Hit
AGCCACAGGGAGGTAGAGTACTAAGCAGGCTCTTGGGGTGGTCTTCAATG9460.10588548697250334No Hit
CATACTCAAAAACTTATGTGTGGAGCTGTATCCTGCCCCCCGAGAGAG9410.10532583852127447No Hit
AGTAAAACTGGCCTAGCCTCCCAGTCCACACCTTTCTCCCATGCTGGAT9410.10532583852127447No Hit
ATGTATGGTAGACCTAGATGAAAGATTTGTTGTCTTAACTCAGAACTGAT9320.1043184713090625No Hit
TATCTTTTTGGGCTGAAACTTGTGGTTTTGAGAAACCAAGTTACTGTTTT9320.1043184713090625No Hit
ATGTGAATTTTAACAGAAAGCGTAATTCCTTCATTAAAAATATCTGAACT9270.10375882285783362No Hit
CTCAAATTGTCGATGGATTGAAAGACGGCAAAGAGGTCTAAACTAGGAG9240.10342303378709629No Hit
CACTGGACTAATTACAGGTTTTCCTTACATGTGTGGGTTCCTAACCTTGA9230.10331110409685053No Hit
AAGGATTCTGAAGTAAAGGAGGATACAGAATGTGACAATAGTGGTCCTCA9230.10331110409685053No Hit
CATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAACTAC9220.10319917440660474No Hit
AGGTTTTCTGGTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA9210.10308724471635898No Hit
AGTTGCAGAGTGCTCATTTAAAATGCTAAAATAGATACAAACTCAATTTT9150.10241566657488432No Hit
AAGGCTGCGGTTTGTTGGATCTTCAATGGCTTTTTAAATTTTATTTCCTG9090.10174408843340967No Hit
ATCGGAATGAGTTTCTCCGCAAAAGCCCTTATGACATTGGGTGCAGCATC9020.10096058060168923No Hit
ATGCCTGGAGCTGTATCCTGCCCCGCAGGAAAGTTATGGTGCTGATGG8980.10051286184070614No Hit
ATCCAAGTAATTGTGACTTTCCTGCCTGGTTGTGACATATTGCTGAACCC8970.10040093215046036No Hit
TGAAACCATGTCTCAGGAAACAAACAATGGTTTGTGTCATCTGAGATGCA8960.10028900246021458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA151.6238411E-4135.2029144
TTTAGTC500.0135.2029144
GTTTGAC1500.0135.2029144
ACTATGG151.6238411E-4135.2029144
GCAAGGC850.0135.2029144
TCCCTCC100.0087364325135.2029144
AGGACGC100.0087364325135.2029144
ATTAAGT352.0008883E-11135.2029144
TTATCCC900.0135.2029144
AGTATAA151.6238411E-4135.2029144
GTCAGAA255.623224E-8135.2029144
GATGGTA2100.0135.2029144
TGTCTAC600.0135.2029144
TCCACAT255.623224E-8135.2029144
TAAGGCA450.0135.2029144
TTTGACT203.0135106E-6135.2029144
AAGGATA1350.0135.2029144
TGCGTAG100.0087364325135.2029144
GGGTATA1100.0135.202944
TGTATTC950.0135.202944
Produced by FastQC (version 0.11.5)