FastQCFastQC Report
Tue 24 May 2016
ERR841091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7153254
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC282310.39465954934635344No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA218320.3052037576185607No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA209310.29260809136653054No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA168070.23495600743381964No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG148430.20749997134171383No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT147440.2061159858156861No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG142930.199811162863782No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT139500.19501614230390812No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG134780.1884177466646648No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA132530.18527232501460175No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA120220.16806337367581242No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA103100.14413020983177727No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC99250.13874804389722495No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG98570.13779742757631702No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG92490.12929779929525778No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA89210.12471247351205478No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC82240.11496865622274842No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT75670.10578402500456435No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC73860.10325370803273587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT156.7182475E-5168.7706344
AGCGATA1550.0141.5495844
TCGGTGC1100.0138.0850844
CACCCGC600.0126.5779744
AGGTGCG1250.0114.76404644
GCGTACT900.0107.2253643
TTACGTT2050.0107.0252844
GAATACA63650.0103.94044544
CGGGTTA1400.0103.39587443
CTATCAT93700.0101.8567844
CGTTGTC2000.0101.2623944
ATTCGGG1600.0100.2075744
ACGATGC3100.097.9958644
CGCGCCT950.095.9384843
GCGGTCC1700.094.3130144
GTAGATA2600.094.1220944
CCTCGGT1200.093.8221843
ATCCCGG900.093.7614744
TCGTCCA750.092.92863543
CTCGGTG1950.090.87649544
Produced by FastQC (version 0.11.5)