##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841091.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7153254
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.284521841388546	39.0	35.0	39.0	31.0	39.0
2	37.1339690999369	39.0	37.0	39.0	33.0	39.0
3	37.25674804781153	39.0	37.0	39.0	33.0	39.0
4	37.47347500871631	39.0	35.0	39.0	34.0	41.0
5	38.374184671759174	40.0	37.0	41.0	34.0	41.0
6	38.37226386760487	40.0	36.0	41.0	34.0	41.0
7	38.36888163065368	40.0	36.0	41.0	34.0	41.0
8	38.248511376780414	40.0	36.0	41.0	34.0	41.0
9	38.18018946342462	40.0	36.0	41.0	34.0	41.0
10	38.05976496850245	40.0	36.0	41.0	34.0	41.0
11	38.00531632177468	40.0	36.0	41.0	34.0	41.0
12	37.994709260988074	39.0	36.0	41.0	34.0	41.0
13	37.95741546434672	39.0	35.0	41.0	34.0	41.0
14	37.95213325292238	39.0	35.0	41.0	34.0	41.0
15	37.81121374971447	39.0	35.0	41.0	34.0	41.0
16	37.895316453183405	39.0	35.0	41.0	34.0	41.0
17	38.07764955641167	40.0	35.0	41.0	34.0	41.0
18	38.05711708265916	40.0	35.0	41.0	34.0	41.0
19	38.04712009387616	40.0	35.0	41.0	34.0	41.0
20	38.034481649889685	40.0	35.0	41.0	34.0	41.0
21	38.05500028378693	40.0	35.0	41.0	34.0	41.0
22	38.020436852934345	40.0	35.0	41.0	34.0	41.0
23	37.95325595875667	40.0	35.0	41.0	34.0	41.0
24	37.86741963867074	39.0	35.0	41.0	34.0	41.0
25	37.830040051433436	39.0	35.0	41.0	34.0	41.0
26	37.77983889691101	39.0	35.0	41.0	34.0	41.0
27	37.68732631966514	39.0	35.0	41.0	34.0	41.0
28	37.57203466223917	39.0	35.0	41.0	34.0	41.0
29	37.45868244020045	39.0	35.0	41.0	34.0	41.0
30	37.427589151001214	39.0	35.0	41.0	34.0	41.0
31	37.27073361795636	38.0	35.0	40.0	33.0	41.0
32	37.14438048817003	38.0	35.0	40.0	33.0	41.0
33	36.90277407056214	37.0	35.0	40.0	33.0	41.0
34	36.859830369533604	37.0	35.0	40.0	33.0	41.0
35	36.84464501926252	37.0	35.0	40.0	33.0	41.0
36	36.82753734509233	37.0	35.0	40.0	33.0	41.0
37	36.70552397900675	37.0	35.0	40.0	33.0	41.0
38	36.59454196766281	36.0	35.0	40.0	33.0	41.0
39	36.53627550662455	36.0	35.0	40.0	33.0	41.0
40	36.44852350879176	36.0	35.0	40.0	33.0	41.0
41	36.30218594092578	36.0	35.0	39.0	33.0	41.0
42	36.22093335633892	36.0	35.0	39.0	33.0	41.0
43	36.09862200454348	36.0	35.0	39.0	33.0	41.0
44	35.97230965046717	35.0	35.0	39.0	33.0	41.0
45	35.83676021837959	35.0	35.0	39.0	32.0	41.0
46	35.582901742272185	35.0	34.0	39.0	32.0	40.0
47	35.39639426340201	35.0	34.0	38.0	31.0	40.0
48	35.267309540966764	35.0	34.0	38.0	31.0	40.0
49	35.45332742408906	35.0	34.0	38.0	32.0	40.0
50	35.22718621043113	36.0	34.0	38.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	14.0
22	76.0
23	223.0
24	686.0
25	1643.0
26	3769.0
27	8519.0
28	17614.0
29	34745.0
30	63508.0
31	111287.0
32	191316.0
33	350145.0
34	838368.0
35	495940.0
36	678627.0
37	948849.0
38	1182155.0
39	1251007.0
40	974759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.3251924229169	15.362672708112978	30.31213486897012
2	27.161484829142097	25.000747911370127	27.39490307488033	20.442864184607455
3	29.000899450795398	22.444428787234454	22.111559298747117	26.443112463223027
4	20.86239353446697	23.23078419975021	29.273404802905084	26.633417462877734
5	16.976078299470423	30.622245484362782	29.27713736992982	23.124538846236973
6	23.2796570623663	29.526198846007706	26.40475229874404	20.789391792881954
7	24.77254407574511	28.684218399067053	24.295879889068665	22.24735763611917
8	25.730471754532974	26.00963142088901	24.867158358979005	23.392738465599013
9	21.982904563433646	29.964796440892492	27.212901988381795	20.839397007292064
10	26.0635369581452	25.546401679571286	27.09153065164469	21.298530710638822
11	23.725719791300577	27.321495923393748	26.501351692530424	22.451432592775262
12	22.42206134438956	25.994253803933148	28.42503565510186	23.158649196575432
13	22.555539059566456	25.850990332511607	25.812783944202177	25.780686663719756
14	17.919942448569557	29.03351397839361	26.646138945995766	26.400404627041063
15	21.30101908865532	31.567857089934176	27.184844827263227	19.946278994147278
16	20.337611386370455	33.79136264418962	23.318772128041307	22.552253841398613
17	23.18962810491561	35.720498670954505	21.46172916549587	19.628144058634014
18	20.58604377811832	30.421945033686765	24.267389358744985	24.724621829449926
19	24.967741953522133	28.3404028432375	23.71619964843972	22.975655554800653
20	24.025108013779466	25.979057922450398	27.034507652041995	22.961326411728145
21	20.157567451120848	32.37722300927662	24.23646636901192	23.228743170590615
22	24.63316694751787	28.483973866998152	22.994500125397476	23.8883590600865
23	25.176248459791868	26.68919627347218	25.611616755115925	22.522938511620026
24	21.405628822910526	29.908444464575147	25.334665873740818	23.351260838773516
25	21.88267749140374	27.890912746648144	27.251130291242887	22.975279470705225
26	22.25988844531272	31.92989608992327	26.98244570910841	18.827769755655595
27	24.76277626031038	29.115368984639357	24.737378074860157	21.38447668019011
28	24.277053338236264	27.57948906083435	24.621372837931546	23.52208476299784
29	22.932671462282812	30.441798064509733	24.082108470236555	22.543422002970896
30	23.926254688520274	30.94294171343337	23.085226438511864	22.045577159534496
31	22.35266107655502	28.284474894432737	26.623537283035446	22.739326745976797
32	21.871167055468675	29.46584684154645	26.93666376069896	21.726322342285915
33	20.262053505934226	26.209271872904523	30.7620741721369	22.766600449024352
34	20.066353316341672	29.754369751557086	26.99578191620999	23.18349501589125
35	20.00675716993824	28.10384944658472	27.112288265150124	24.77710511832691
36	21.06856463533191	28.166340946914108	27.281250849895773	23.483843567858216
37	19.415989836851548	32.346683154018955	24.331521259582615	23.90580574954688
38	21.77200014565459	27.565629375667775	27.816354458539948	22.84601602013768
39	21.380617613882023	29.97631629566051	28.244469830817064	20.398596259640403
40	19.221669123429987	28.8036112066083	29.072505129376452	22.90221454058526
41	19.645443044003645	29.663608225082967	26.047573281318066	24.64337544959533
42	20.12864201305609	31.96986670506569	25.055790650895155	22.845700630983064
43	20.900715896976518	26.774639567644105	27.899248578125547	24.425395957253837
44	21.101141659943522	28.92156157523112	25.5277148389606	24.449581925864763
45	19.865381022564506	28.542507749288013	28.685322992467338	22.90678823568014
46	18.32274722848282	32.65470502825362	26.29394583789127	22.728601905372283
47	20.633262154499032	28.174422671884873	30.63392054263192	20.558394630984175
48	21.58905378073446	30.477597867131358	25.21868020546913	22.714668146665055
49	18.485518282663442	33.113339179979675	24.324702957188652	24.076439580168227
50	21.434508678862926	30.61145262339361	26.37461230092223	21.579426396821237
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39257.0
1	38281.0
2	30368.5
3	23183.5
4	22111.5
5	17698.0
6	12270.5
7	11408.5
8	13378.5
9	15396.5
10	18577.5
11	23332.5
12	26855.0
13	28556.5
14	30753.0
15	33582.5
16	35520.5
17	36225.5
18	35287.5
19	32365.0
20	31507.0
21	40888.0
22	53905.0
23	61849.0
24	67695.5
25	82890.0
26	96385.0
27	100225.0
28	111238.5
29	128532.5
30	159945.0
31	224873.0
32	292604.5
33	288969.0
34	253415.0
35	262216.0
36	289638.0
37	312263.0
38	339160.0
39	387109.0
40	446602.0
41	484328.5
42	520192.0
43	588774.5
44	644900.5
45	657455.0
46	658560.0
47	663807.0
48	675319.0
49	695741.0
50	750204.5
51	773316.0
52	704871.0
53	592047.0
54	507547.0
55	444469.5
56	378970.0
57	322881.5
58	268147.0
59	225545.0
60	181100.5
61	127064.5
62	86358.5
63	63941.5
64	55515.5
65	52292.0
66	36178.5
67	19141.0
68	11064.5
69	8517.0
70	5866.5
71	3720.5
72	2196.5
73	1379.0
74	722.5
75	454.5
76	288.0
77	202.5
78	110.5
79	56.0
80	32.5
81	24.5
82	21.5
83	18.0
84	15.0
85	13.5
86	7.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	41149.0
25	48305.0
26	23927.0
27	39979.0
28	37007.0
29	36128.0
30	67862.0
31	99244.0
32	154666.0
33	34705.0
34	29082.0
35	33049.0
36	36928.0
37	45061.0
38	26403.0
39	29589.0
40	29468.0
41	34986.0
42	45318.0
43	74055.0
44	212891.0
45	443719.0
46	866830.0
47	641277.0
48	1359131.0
49	964980.0
50	1697515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.96859663801746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3608610890989	7.717599043899039
2	12.046657792986556	2.642698603349984
3	4.92738237343244	1.6213940920637266
4	2.5654420643203077	1.1255719680692924
5	1.5213070268864552	0.8343301570249557
6	1.0278598969084745	0.6764508365749962
7	0.7571916211433276	0.5813730629005387
8	0.5657090573180795	0.4964027571356092
9	0.4486009340409192	0.4428470427239447
>10	3.687937598958843	8.221579262227484
>50	0.5393561012302179	4.210030422057421
>100	1.2796945832034095	37.33736461702853
>500	0.2100875112789619	14.441777596562197
>1k	0.05517711455292095	11.055355282388536
>5k	0.0036266648062952735	3.063214499036095
>10k+	0.003108569833967377	5.53201075695765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	28231	0.39465954934635344	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	21832	0.3052037576185607	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAATA	20931	0.29260809136653054	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	16807	0.23495600743381964	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	14843	0.20749997134171383	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	14744	0.2061159858156861	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	14293	0.199811162863782	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	13950	0.19501614230390812	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	13478	0.1884177466646648	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	13253	0.18527232501460175	No Hit
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAA	12022	0.16806337367581242	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	10310	0.14413020983177727	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	9925	0.13874804389722495	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	9857	0.13779742757631702	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	9249	0.12929779929525778	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	8921	0.12471247351205478	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	8224	0.11496865622274842	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	7567	0.10578402500456435	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	7386	0.10325370803273587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.051025728989911444	0.0	0.0	0.0	0.0
2	0.14427000634955783	0.0	0.0	0.0	0.0
3	0.2893787918057992	0.0	0.0	0.0	0.0
4	0.32681629926743827	0.0	0.0	0.0	0.0
5	0.42210160578668116	0.0	0.0	0.0	0.0
6	0.5027222575907412	0.0	0.0	0.0	0.0
7	0.5954632674863776	0.0	0.0	0.0	0.0
8	0.7576132484600715	0.0	0.0	0.0	0.0
9	0.8356057257298567	0.0	0.0	0.0	0.0
10	0.8931739317518992	0.0	0.0	0.0	0.0
11	0.9800434879007511	0.0	0.0	0.0	0.0
12	1.0394709876092754	0.0	0.0	0.0	0.0
13	1.0966198040779762	0.0	0.0	0.0	0.0
14	1.1567323067236253	0.0	0.0	0.0	0.0
15	1.2236249404816326	0.0	0.0	0.0	0.0
16	1.2835137686988327	0.0	0.0	0.0	0.0
17	1.4318658333675836	0.0	0.0	0.0	0.0
18	1.4859810654004457	0.0	0.0	0.0	0.0
19	1.5710752057734843	0.0	0.0	0.0	0.0
20	1.624771048253005	0.0	0.0	0.0	0.0
21	1.6816961902932568	6.989825889028965E-5	0.0	0.0	0.0
22	1.716267869140394	6.989825889028965E-5	0.0	0.0	0.0
23	1.7735285228233193	6.989825889028965E-5	0.0	0.0	0.0
24	1.8270705891332812	6.989825889028965E-5	0.0	0.0	0.0
25	1.8640188087826883	6.989825889028965E-5	0.0	0.0	0.0
26	1.9477848822368113	6.989825889028965E-5	0.0	0.0	0.0
27	1.980035938888791	6.989825889028965E-5	0.0	0.0	0.0
28	2.0310337085751464	6.989825889028965E-5	0.0	0.0	0.0
29	2.0756567570507074	6.989825889028965E-5	0.0	0.0	0.0
30	2.1049021885704047	6.989825889028965E-5	0.0	0.0	0.0
31	2.132022713019837	6.989825889028965E-5	0.0	0.0	0.0
32	2.1611003887181974	6.989825889028965E-5	0.0	0.0	0.0
33	2.1883607096854103	6.989825889028965E-5	0.0	0.0	0.0
34	2.2185707371777936	6.989825889028965E-5	0.0	0.0	0.0
35	2.246851572724805	6.989825889028965E-5	0.0	0.0	0.0
36	2.2690792190519167	6.989825889028965E-5	0.0	0.0	0.0
37	2.286777458202938	6.989825889028965E-5	0.0	0.0	0.0
38	2.300281801820542	6.989825889028965E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGT	15	6.7182475E-5	168.77063	44
AGCGATA	155	0.0	141.54958	44
TCGGTGC	110	0.0	138.08508	44
CACCCGC	60	0.0	126.57797	44
AGGTGCG	125	0.0	114.764046	44
GCGTACT	90	0.0	107.22536	43
TTACGTT	205	0.0	107.02528	44
GAATACA	6365	0.0	103.940445	44
CGGGTTA	140	0.0	103.395874	43
CTATCAT	9370	0.0	101.85678	44
CGTTGTC	200	0.0	101.26239	44
ATTCGGG	160	0.0	100.20757	44
ACGATGC	310	0.0	97.99586	44
CGCGCCT	95	0.0	95.93848	43
GCGGTCC	170	0.0	94.31301	44
GTAGATA	260	0.0	94.12209	44
CCTCGGT	120	0.0	93.82218	43
ATCCCGG	90	0.0	93.76147	44
TCGTCCA	75	0.0	92.928635	43
CTCGGTG	195	0.0	90.876495	44
>>END_MODULE