FastQCFastQC Report
Tue 24 May 2016
ERR841090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191074
Sequences flagged as poor quality0
Sequence length24-50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA1003831.93376168399834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA946521.8233606378949712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA944921.8202784240794874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA800861.5427635976678429No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA738251.4221527183006832No Hit
AAAAAAAAAAAAAAAAAAAAAAAA626001.2059161553081308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA472970.9111216676934292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265500.511454855006883No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA206060.39695061176165086No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA188440.36300773211863285No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA185180.3567277214695841No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA150900.2906912904728386No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149890.28874564300181427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135730.2614680507347805No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA113650.21893350008110077No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA108690.20937863725310024No Hit
AAGCAGTAGGCAGAGGCTGGTGGG107660.2073944621093824No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA107040.2062001042558823No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA101590.1957013134468898No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98240.1892479282707201No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA97370.18757197450855065No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA87350.16826961048908182No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG85670.1650332859828236No Hit
TAAAAAAAAAAAAAAAAAAAAAAA84390.16256751493043636No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA82440.15881106684281518Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA75580.14559607510892736No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA75050.14457509178254827Illumina Single End Adapter 1 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74940.14436318958273375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG72790.14022146476817707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG72640.13993250722297543No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA71020.13681176573479784Illumina Single End Adapter 1 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAA67980.13095555948537818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66800.1286824267964587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG65960.12706426454332959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG64040.12336560796474873No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA63420.12217125011124866Illumina Single End Adapter 1 (100% over 32bp)
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA60730.11698927813396612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG57360.11049736528510284No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56050.1079738027236753No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT55460.10683723637921556No Hit
AGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA52980.1020598049652153No Hit
CAAAAAAAAAAAAAAAAAAAAAAA51980.10013342133053776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT450.0231.0937744
ATTCGGC600.0231.0937744
GCTACGA1400.0231.0937744
TAGTGTC100.0017583157231.0937744
TATCGTT800.0231.0937744
GCCTGTC850.0231.0937544
ATTCCGA1000.0219.5390644
AGACTAA900.0218.2552244
TATTGGC500.0207.9843844
CGGTGAA1450.0207.1875244
GCGACAA750.0184.87544
AAGACCG2250.0184.87544
TGTGCGC900.0179.739644
TAAGGGC206.0412458E-5173.3203344
ACGACAC1550.0171.4566544
TAGTAAT2450.0169.7831644
GGCACGC1200.0154.0625244
GGCCACG301.5754285E-6154.0625244
GCGCGCG750.0154.062544
TGGGATA1900.0145.9539544
Produced by FastQC (version 0.11.5)