##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841090.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5191074
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.999192074703615	39.0	37.0	39.0	33.0	39.0
2	37.6851339433805	39.0	37.0	39.0	35.0	39.0
3	37.83707648937388	39.0	37.0	39.0	35.0	39.0
4	38.26315344377676	39.0	38.0	40.0	35.0	41.0
5	39.01736731165843	40.0	38.0	41.0	35.0	41.0
6	38.86503794783122	40.0	38.0	41.0	35.0	41.0
7	38.93439430838397	40.0	38.0	41.0	35.0	41.0
8	38.83575787977594	40.0	38.0	41.0	35.0	41.0
9	38.863078815674754	40.0	38.0	41.0	35.0	41.0
10	38.878188213074985	40.0	38.0	41.0	35.0	41.0
11	38.77546919962998	40.0	38.0	41.0	35.0	41.0
12	38.7342756046244	40.0	38.0	41.0	35.0	41.0
13	38.638485407836605	40.0	38.0	41.0	35.0	41.0
14	38.580948759351145	40.0	38.0	41.0	35.0	41.0
15	38.27710489197418	39.0	37.0	41.0	35.0	41.0
16	38.040616257830266	39.0	36.0	41.0	34.0	41.0
17	38.023113521402315	39.0	35.0	41.0	34.0	41.0
18	38.02787650493905	40.0	35.0	41.0	34.0	41.0
19	37.98439051340821	40.0	35.0	41.0	34.0	41.0
20	38.022946311302825	40.0	35.0	41.0	34.0	41.0
21	37.907672477795536	40.0	35.0	41.0	34.0	41.0
22	37.914087527937376	40.0	35.0	41.0	34.0	41.0
23	37.88214404186879	40.0	35.0	41.0	34.0	41.0
24	37.626884532950214	40.0	35.0	41.0	33.0	41.0
25	37.59460282168905	40.0	35.0	41.0	33.0	41.0
26	37.6244226176496	40.0	35.0	41.0	33.0	41.0
27	37.671922505906764	40.0	35.0	41.0	33.0	41.0
28	37.7531628690444	40.0	36.0	41.0	33.0	41.0
29	37.89031536194188	40.0	37.0	41.0	33.0	41.0
30	38.14431722014523	40.0	37.0	41.0	34.0	41.0
31	38.172279758115025	40.0	37.0	41.0	34.0	41.0
32	38.14445091186586	40.0	37.0	41.0	34.0	41.0
33	37.964825398987514	40.0	36.0	41.0	33.0	41.0
34	38.070459440619096	40.0	36.0	41.0	34.0	41.0
35	38.04283100309417	40.0	36.0	41.0	34.0	41.0
36	38.00838963096205	40.0	35.0	41.0	34.0	41.0
37	37.93459045449996	40.0	35.0	41.0	34.0	41.0
38	37.8472638067257	40.0	35.0	41.0	33.0	41.0
39	37.81920752684408	39.0	35.0	41.0	33.0	41.0
40	37.693843156800284	39.0	35.0	41.0	33.0	41.0
41	37.57453072821662	39.0	35.0	41.0	33.0	41.0
42	37.497617872080525	39.0	35.0	41.0	33.0	41.0
43	37.48311433846772	39.0	35.0	41.0	33.0	41.0
44	37.410011851244604	39.0	35.0	41.0	33.0	41.0
45	37.22221028887434	39.0	35.0	41.0	33.0	41.0
46	36.96818403097111	39.0	35.0	41.0	32.0	41.0
47	36.76531126869151	38.0	35.0	40.0	31.0	41.0
48	36.73580800315613	38.0	35.0	40.0	31.0	41.0
49	36.892835513251505	38.0	35.0	40.0	32.0	41.0
50	36.509513479408476	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	77.0
21	188.0
22	413.0
23	711.0
24	1389.0
25	2338.0
26	4233.0
27	7606.0
28	13132.0
29	22554.0
30	38475.0
31	65833.0
32	109587.0
33	192923.0
34	298035.0
35	275861.0
36	525526.0
37	832334.0
38	738242.0
39	878361.0
40	1183248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.883618688541134	18.15828477883382	25.958096532625042
2	23.788815185451025	40.68666715211534	22.627417755940293	12.89709990649334
3	25.85516985502422	33.58436038476816	20.82908084145978	19.731388918747836
4	19.3979511754215	38.22534604592421	23.771747426447785	18.6049553522065
5	19.54104295180535	39.940906255622636	21.241365466953464	19.276685325618555
6	19.658109285284702	42.047098538760956	23.137466350893863	15.157325825060477
7	24.75582124238645	37.77855988953346	20.443033561070408	17.022585307009685
8	18.36569850477955	39.52937291974648	21.837600465722506	20.26732810975147
9	22.68717417628799	41.856328767418844	19.689220380984747	15.767276675308423
10	21.883178702518975	36.61011189591981	22.176143125680735	19.330566275880482
11	20.394353846622106	38.660901385724806	23.789238989850656	17.155505777802436
12	19.01737482455461	36.1869046752175	26.29981772558049	18.495902774647405
13	19.58847051689111	41.14044608109998	18.667890305551413	20.603193096457495
14	17.408690378908105	37.556043315891856	28.288654717694257	16.746611587505782
15	21.66436078545596	40.26378741663093	23.025177448828508	15.046674349084602
16	19.617173633047805	43.20928578556191	21.369643353186643	15.803897228203642
17	21.58131438696501	42.98963952353598	19.90489829272324	15.524147796775772
18	23.707175047013394	38.91553077455648	19.557301629682026	17.8199925487481
19	22.21547987950085	39.03234667816333	21.29266506314493	17.459508379190897
20	23.27618523642699	39.379249843096055	21.021064234491746	16.323500685985213
21	22.349498388965365	41.23079347356636	20.175497401886393	16.244210735581884
22	21.43408088576661	41.86578731106511	19.99855906504126	16.701572738127023
23	22.978135160469684	42.82709127244188	18.705974910008987	15.488798657079442
24	21.64696554123482	43.82788609832956	19.71988840844881	14.805259951986816
25	20.501252066924444	42.56373845643126	20.32790410021891	16.607105376425384
26	21.92951233941648	43.665299431717756	20.406082500603308	13.999105728262457
27	22.283085678127364	41.46638268186038	19.87660131218941	16.373930327822848
28	22.328638138187223	38.31708525680621	20.46719958981206	18.887077015194503
29	21.832391558050713	39.930633164024	21.973607710575745	16.263367567349544
30	24.316029572179758	37.255306209413064	20.360500003907287	18.068164214499895
31	20.297555716282236	36.139890887246764	22.757880556101696	20.804672840369307
32	19.858371582236806	35.0995720632702	25.60901777010556	19.43303858438744
33	19.457805652554725	36.554537683280856	25.007173737660043	18.98048292650437
34	21.31379654461726	36.25463950778438	24.305922568615248	18.12564137898311
35	21.25972612175832	35.77325620676044	22.73416985896647	20.232847812514766
36	20.92940222409817	36.14973546312291	22.034959607042424	20.88590270573649
37	19.590934340114302	39.1768001274085	22.83377899720355	18.398486535273655
38	19.078559216030122	38.08937192236821	23.198844831161942	19.633224030439727
39	19.976291841269227	37.524378183269604	24.8653812295956	17.633948745865567
40	19.19778815665416	37.0553005595694	23.989053471223368	19.75785781255307
41	18.590350990800854	38.441605205623794	23.98064379384194	18.987400009733417
42	19.047310268562825	39.60442712292168	22.717646473221073	18.630616135294424
43	18.218411073416966	39.442987996955836	20.70328579648873	21.63531513313847
44	17.877585776724334	40.44913877985645	21.341847145676745	20.33142829774247
45	19.680419122462347	36.43580004365859	24.101346139853018	19.782434694026048
46	19.682395842431653	36.59062825933937	23.31313934430441	20.413836553924565
47	17.92046185210128	38.60671111693488	23.829485581596614	19.643341449367224
48	18.362857553702188	37.49737296197155	23.372803427501125	20.766966056825137
49	17.225056565744655	37.615233886982026	21.72920564321602	23.430503904057296
50	17.16043863954991	38.532240835183735	24.753804234628067	19.55351629063829
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	770072.0
1	760518.5
2	465755.5
3	179362.5
4	174182.5
5	130577.0
6	59561.5
7	28206.0
8	24992.5
9	17973.5
10	12128.0
11	10039.5
12	10308.5
13	11479.5
14	14228.5
15	17320.5
16	20042.0
17	24678.5
18	33121.0
19	44362.0
20	57509.5
21	74824.0
22	87598.5
23	92794.0
24	97228.5
25	104085.0
26	111672.5
27	116582.5
28	117230.5
29	113694.5
30	113975.0
31	124973.0
32	140117.5
33	150297.0
34	171850.5
35	197688.0
36	206134.0
37	209397.0
38	218474.5
39	231476.5
40	255092.0
41	277118.5
42	284882.0
43	299358.0
44	308895.5
45	306051.0
46	331349.0
47	353658.5
48	346905.5
49	355948.0
50	372830.5
51	369047.0
52	344811.0
53	305318.0
54	279854.5
55	257868.0
56	226006.0
57	194046.0
58	159734.5
59	133146.0
60	115800.5
61	104192.5
62	91405.5
63	78230.5
64	68452.0
65	57941.5
66	40813.5
67	25751.0
68	16595.5
69	11061.5
70	7820.0
71	6373.0
72	5823.5
73	4145.5
74	2285.5
75	1834.5
76	1581.5
77	1113.5
78	602.0
79	275.5
80	190.5
81	88.5
82	27.0
83	16.5
84	14.5
85	10.0
86	4.0
87	1.5
88	1.0
89	1.0
90	5.5
91	10.0
92	10.0
93	10.0
94	5.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	112272.0
25	122814.0
26	145402.0
27	161010.0
28	162129.0
29	136601.0
30	124209.0
31	117151.0
32	115752.0
33	31568.0
34	25088.0
35	24391.0
36	25976.0
37	34114.0
38	24379.0
39	30119.0
40	37858.0
41	43606.0
42	53231.0
43	72220.0
44	155434.0
45	321622.0
46	488872.0
47	455512.0
48	724965.0
49	623786.0
50	820993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.993127362973736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06322662093785	8.03193355793783
2	11.20562668962751	2.4636976276202596
3	4.208240007513508	1.3878515512967264
4	2.2591270323824526	0.9933948478446877
5	1.3628092477817941	0.7490767816151848
6	0.9515537698313344	0.6276331070684192
7	0.6934638869423917	0.5336335781546372
8	0.5285562986640646	0.46483893677728366
9	0.42509341097886494	0.420579540724644
>10	3.7951750482974416	8.373902715536358
>50	0.4776286374460003	3.702614307055664
>100	0.8098879934571969	23.17446841864361
>500	0.1649929877732188	11.697969150486122
>1k	0.04238612961760276	9.090991560092036
>5k	0.0068272960457883645	5.2908223046928375
>10k+	0.005404942702915789	22.996592014453693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	100383	1.93376168399834	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	94652	1.8233606378949712	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	94492	1.8202784240794874	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	80086	1.5427635976678429	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73825	1.4221527183006832	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	62600	1.2059161553081308	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47297	0.9111216676934292	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26550	0.511454855006883	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	20606	0.39695061176165086	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18844	0.36300773211863285	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	18518	0.3567277214695841	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	15090	0.2906912904728386	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14989	0.28874564300181427	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13573	0.2614680507347805	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	11365	0.21893350008110077	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA	10869	0.20937863725310024	No Hit
AAGCAGTAGGCAGAGGCTGGTGGG	10766	0.2073944621093824	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10704	0.2062001042558823	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA	10159	0.1957013134468898	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9824	0.1892479282707201	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9737	0.18757197450855065	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA	8735	0.16826961048908182	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	8567	0.1650332859828236	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	8439	0.16256751493043636	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	8244	0.15881106684281518	Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	7558	0.14559607510892736	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7505	0.14457509178254827	Illumina Single End Adapter 1 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7494	0.14436318958273375	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG	7279	0.14022146476817707	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG	7264	0.13993250722297543	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7102	0.13681176573479784	Illumina Single End Adapter 1 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAA	6798	0.13095555948537818	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6680	0.1286824267964587	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG	6596	0.12706426454332959	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG	6404	0.12336560796474873	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6342	0.12217125011124866	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	6073	0.11698927813396612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG	5736	0.11049736528510284	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5605	0.1079738027236753	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5546	0.10683723637921556	No Hit
AGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA	5298	0.1020598049652153	No Hit
CAAAAAAAAAAAAAAAAAAAAAAA	5198	0.10013342133053776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3618711657741731	0.0	0.0	0.0	0.0
2	0.6226842460731633	0.0	0.0	0.0	0.0
3	0.8173453123573272	0.0	0.0	0.0	0.0
4	1.0201357175798302	0.0	0.0	0.0	0.0
5	1.1848800460174522	0.0	0.0	0.0	0.0
6	1.418993449139812	0.0	0.0	0.0	0.0
7	1.5679414317730782	0.0	0.0	0.0	0.0
8	1.7499461575774107	0.0	0.0	0.0	0.0
9	1.9084875307113711	0.0	0.0	0.0	0.0
10	2.0612112252686052	0.0	0.0	0.0	0.0
11	2.2655620012351974	0.0	0.0	0.0	0.0
12	2.4023545031336484	0.0	0.0	0.0	0.0
13	2.5615123190307054	0.0	0.0	0.0	0.0
14	2.688538055901341	0.0	0.0	0.0	0.0
15	2.832593024102527	0.0	0.0	0.0	0.0
16	2.956440227975945	0.0	0.0	0.0	0.0
17	3.1283892312072608	0.0	0.0	0.0	0.0
18	3.22582571544925	0.0	0.0	0.0	0.0
19	3.3408308184394984	0.0	0.0	0.0	0.0
20	3.459226356626779	0.0	0.0	0.0	0.0
21	3.555622593706042	0.0	0.0	0.0	0.0
22	3.654291963474225	0.0	0.0	0.0	0.0
23	3.7649049117774087	0.0	0.0	0.0	0.0
24	3.8615130510564866	0.0	0.0	0.0	0.0
25	3.9580248711538304	0.0	0.0	0.0	0.0
26	4.069581747438006	0.0	0.0	0.0	0.0
27	4.142110091283615	0.0	0.0	0.0	0.0
28	4.220244211506135	0.0	0.0	0.0	0.0
29	4.291404822971123	0.0	0.0	0.0	0.0
30	4.352124435136159	0.0	0.0	0.0	0.0
31	4.408837169341065	0.0	0.0	0.0	0.0
32	4.459751488805592	0.0	0.0	0.0	0.0
33	4.5092595482168045	0.0	0.0	0.0	0.0
34	4.557592513610864	0.0	0.0	0.0	0.0
35	4.5977768762302365	0.0	0.0	0.0	0.0
36	4.631758283545948	0.0	0.0	0.0	0.0
37	4.6510221198927235	0.0	0.0	0.0	0.0
38	4.667839449023458	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGT	45	0.0	231.09377	44
ATTCGGC	60	0.0	231.09377	44
GCTACGA	140	0.0	231.09377	44
TAGTGTC	10	0.0017583157	231.09377	44
TATCGTT	80	0.0	231.09377	44
GCCTGTC	85	0.0	231.09375	44
ATTCCGA	100	0.0	219.53906	44
AGACTAA	90	0.0	218.25522	44
TATTGGC	50	0.0	207.98438	44
CGGTGAA	145	0.0	207.18752	44
GCGACAA	75	0.0	184.875	44
AAGACCG	225	0.0	184.875	44
TGTGCGC	90	0.0	179.7396	44
TAAGGGC	20	6.0412458E-5	173.32033	44
ACGACAC	155	0.0	171.45665	44
TAGTAAT	245	0.0	169.78316	44
GGCACGC	120	0.0	154.06252	44
GGCCACG	30	1.5754285E-6	154.06252	44
GCGCGCG	75	0.0	154.0625	44
TGGGATA	190	0.0	145.95395	44
>>END_MODULE