FastQCFastQC Report
Tue 24 May 2016
ERR841089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18866042
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA2074761.0997325247129206No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT1102230.5842401919809147No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC914450.4847068611423636No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC795460.4216358682971235No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG608050.32229865702620614No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG545630.2892127559135085No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA518470.2748165195434209No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT439000.23269321673300633No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC370190.1962202776819854No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC328560.1741541760587621No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA314150.16651611397875613No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG301550.15983744762149898No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT298800.15837980218638334No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA278070.1473918058700389No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT217220.11513808778757093No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216660.11484125817169283No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA215830.11440131427673064No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT213460.11314508893810371No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT207880.11018739383703269No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA201150.10662013791764059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG700.0245.3565744
ACTTCTA277500.0137.3554744
CTATCAT313350.0136.7137544
ATCGACA1300.0132.1150744
ACGGTTA850.0129.8946544
AATCGCA14900.0117.7382244
TAGCGTG3350.0113.52318644
GAACACA138250.0100.6272444
ACCACTC41000.094.5520444
CCTTAGC492750.090.7525443
CCAGGCG15500.084.6875944
CGCGTCA2600.082.5951343
CCCATAC123000.079.4915344
GCGATAT501.4097168E-979.1174543
CATGACC37850.078.7604244
CACGCGT1050.075.34994543
TCCGGGC41550.075.21393643
GACTCGT2450.075.1091544
TCGTAAC950.071.3841643
TTGCGTA1450.070.01037642
Produced by FastQC (version 0.11.5)