##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841089.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18866042
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.42023907293326	39.0	37.0	39.0	35.0	39.0
2	37.938928048607124	39.0	38.0	39.0	35.0	39.0
3	38.17868856647303	39.0	38.0	39.0	35.0	40.0
4	38.62836402039177	39.0	38.0	41.0	35.0	41.0
5	39.23168473811306	40.0	39.0	41.0	35.0	41.0
6	39.291671035185864	40.0	39.0	41.0	35.0	41.0
7	39.269286848826056	40.0	39.0	41.0	35.0	41.0
8	39.18665112692954	40.0	38.0	41.0	35.0	41.0
9	39.205384892072225	40.0	39.0	41.0	35.0	41.0
10	39.125675857182976	40.0	39.0	41.0	35.0	41.0
11	39.02738088890081	40.0	38.0	41.0	35.0	41.0
12	38.986582877320004	40.0	38.0	41.0	35.0	41.0
13	38.93210271661645	40.0	38.0	41.0	35.0	41.0
14	38.92648844945855	40.0	38.0	41.0	35.0	41.0
15	38.91991828492696	40.0	38.0	41.0	35.0	41.0
16	39.0006230771669	40.0	38.0	41.0	35.0	41.0
17	39.041270341707076	40.0	38.0	41.0	35.0	41.0
18	39.03191024381267	40.0	38.0	41.0	35.0	41.0
19	39.057654170387195	40.0	39.0	41.0	35.0	41.0
20	39.04485530139284	40.0	38.0	41.0	35.0	41.0
21	39.106437693714454	40.0	38.0	41.0	35.0	41.0
22	39.12946944568447	40.0	38.0	41.0	35.0	41.0
23	39.093089265888416	40.0	38.0	41.0	35.0	41.0
24	39.02880747323683	40.0	38.0	41.0	35.0	41.0
25	39.03218404810908	40.0	38.0	41.0	35.0	41.0
26	39.05366376017212	40.0	38.0	41.0	35.0	41.0
27	38.97424036231241	40.0	38.0	41.0	35.0	41.0
28	38.89592203244971	40.0	38.0	41.0	35.0	41.0
29	38.8707941521701	40.0	38.0	41.0	35.0	41.0
30	38.87920599555659	40.0	38.0	41.0	35.0	41.0
31	38.75907880562781	40.0	38.0	41.0	35.0	41.0
32	38.68633574179568	40.0	38.0	41.0	35.0	41.0
33	38.517388109892096	40.0	38.0	41.0	34.0	41.0
34	38.56304233106781	40.0	37.0	41.0	34.0	41.0
35	38.58511077034004	40.0	37.0	41.0	34.0	41.0
36	38.62508307213503	40.0	37.0	41.0	35.0	41.0
37	38.577269349151194	40.0	37.0	41.0	35.0	41.0
38	38.50601261847125	40.0	37.0	41.0	34.0	41.0
39	38.516887953034676	40.0	37.0	41.0	34.0	41.0
40	38.42053133747318	40.0	37.0	41.0	34.0	41.0
41	38.377274913705115	40.0	37.0	41.0	34.0	41.0
42	38.280269354473226	40.0	36.0	41.0	34.0	41.0
43	38.21365638481109	40.0	36.0	41.0	34.0	41.0
44	38.069126149416824	40.0	36.0	41.0	34.0	41.0
45	37.93899813244135	39.0	36.0	41.0	34.0	41.0
46	37.62970180455796	39.0	35.0	41.0	33.0	41.0
47	37.461092082049724	39.0	35.0	41.0	33.0	41.0
48	37.459489907322684	39.0	35.0	41.0	33.0	41.0
49	37.60457927153208	39.0	36.0	40.0	33.0	41.0
50	37.36136268072581	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	14.0
21	43.0
22	86.0
23	309.0
24	729.0
25	1721.0
26	3625.0
27	8259.0
28	17871.0
29	36945.0
30	74191.0
31	139143.0
32	246906.0
33	450908.0
34	903768.0
35	713033.0
36	1041229.0
37	1598357.0
38	2478280.0
39	4197309.0
40	6953313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.42995239807057	17.497888534330624	32.07215906759881
2	25.91654359722087	21.257686164379365	31.797872600940885	21.02789763745888
3	26.318281280196448	21.903428392664452	25.18875978331862	26.589530543820477
4	20.62758049621643	25.06202413839638	29.630746078059193	24.679649287327994
5	16.96280014642181	26.81102374308294	29.741484726897145	26.484691383598108
6	22.48184860396261	24.69359497874541	27.858853489248038	24.965702928043942
7	22.902514475479276	22.405356672056598	27.77220044352705	26.91992840893707
8	19.986704153420202	23.84667117777009	30.085287629487944	26.08133703932176
9	21.139659288365838	27.45544083915429	29.02437087757994	22.380528994899937
10	28.231305750299928	21.496459087709017	28.963600314257754	21.3086348477333
11	22.208913772162703	21.784357312466494	28.630048634472455	27.37668028089835
12	21.425135171436597	23.406091219345317	29.513752805172384	25.6550208040457
13	22.197310914499184	24.943578520603317	27.0948988664395	25.764211698458002
14	18.434486682474258	25.545723899056306	25.62797220529881	30.391817213170626
15	20.454306207947592	30.345856327469217	28.17141507476767	21.02842238981552
16	20.88026730779037	31.050487431332975	25.92748388877752	22.14176137209914
17	22.819767919524402	34.942215224581815	21.740352321912567	20.497664533981215
18	20.603054949204502	28.230060126019012	24.579628307834785	26.5872566169417
19	25.291521136229843	23.403631773956615	24.969567013579212	26.335280076234323
20	23.098522732007062	23.288218058668587	28.29589269439769	25.317366514926658
21	20.979747633340367	29.950680699216083	26.039600675117757	23.029970992325786
22	24.579787323700437	25.9944454698023	24.274111125163405	25.151656081333858
23	26.329921241561955	23.945170905481923	29.39762881901779	20.327279033938332
24	20.954798044020045	27.109427616030963	24.88404828103319	27.051726058915804
25	20.903942186761668	24.044348165605275	29.092261809823842	25.959447837809215
26	22.913775215087757	30.913173816254535	27.9968904631757	18.17616050548201
27	25.354074948178123	25.14505020437161	24.154438410905207	25.346436436545055
28	23.911975106986667	23.934324518829836	26.348662423679336	25.80503795050416
29	24.660445498353656	27.997274385125497	26.2763888892634	21.065891227257445
30	25.08491213899029	27.851219526868025	26.974173019529264	20.08969531461242
31	23.675089336971087	28.598863927726693	24.842852543545433	22.883194191756782
32	22.495395821661827	28.491727417035467	25.68184786282015	23.331028898482558
33	20.944560777132303	24.04658753275714	31.867165874885924	23.141685815224637
34	21.337783882213394	29.94979377067153	26.193392658322306	22.51902968879277
35	18.722567774639074	25.620398827634443	27.50846539575037	28.14856800197611
36	22.363153232544004	24.77531108450819	28.3971159655244	24.464419717423404
37	18.907187874503176	30.73877819640707	24.19745891062504	26.15657501846471
38	24.079843637288878	25.62569857950418	25.058024050473886	25.236433732733055
39	21.931937377634085	29.418309926511615	26.96568464772383	21.684068048130474
40	17.75091761530085	25.56879241076373	29.871626013324686	26.80866396061073
41	19.740249499110266	30.570917185083385	24.688878518513075	24.999954797293274
42	20.183147223339603	31.473779189289996	25.35326352671558	22.98981006065482
43	18.492898147927825	24.64549638045732	26.126920883183963	30.734684588430895
44	19.044202944066036	28.227142202129546	23.45321637670849	29.27543847709593
45	18.313418275548543	25.684129540908657	30.35740774581856	25.645044437724234
46	17.973971128226967	29.234480676502766	29.56738238025096	23.224165815019308
47	21.65187781294439	29.284693365718212	28.774326308979216	20.28910251235818
48	22.929070558149455	27.64513848442689	23.564085672149808	25.861705285273846
49	17.740192904584305	29.693024810743925	25.071830454205646	27.494951830466125
50	18.73040721932917	30.992269625125335	28.570495196817287	21.70682795872821
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36391.0
1	46553.5
2	126428.5
3	193641.5
4	179096.0
5	112726.5
6	42117.0
7	30037.5
8	37643.0
9	40271.5
10	37144.5
11	31211.0
12	25081.5
13	20110.5
14	17492.0
15	17012.5
16	17429.0
17	20162.0
18	23823.5
19	26492.0
20	30351.5
21	41044.0
22	52987.5
23	67956.0
24	84469.0
25	97323.5
26	116979.0
27	152651.0
28	192661.0
29	230258.0
30	291442.0
31	446925.5
32	686633.0
33	738596.5
34	671526.5
35	751163.5
36	821294.0
37	819432.5
38	855545.5
39	939855.5
40	1031606.0
41	1137253.5
42	1305969.0
43	1478663.5
44	1540356.0
45	1571501.0
46	1621163.5
47	1588246.5
48	1731316.5
49	2047468.5
50	2336478.0
51	2400984.0
52	2024957.5
53	1610260.5
54	1359782.5
55	1158415.0
56	1027703.5
57	946604.5
58	858995.0
59	728040.0
60	585192.0
61	462592.5
62	366929.0
63	286426.0
64	229722.0
65	194179.5
66	131024.0
67	73118.0
68	47539.0
69	34677.5
70	25510.5
71	19850.0
72	15327.0
73	10156.0
74	6404.5
75	4273.0
76	3085.5
77	2411.0
78	2281.5
79	2655.5
80	3210.5
81	2866.0
82	1971.0
83	938.0
84	209.0
85	123.0
86	103.5
87	78.0
88	57.5
89	55.5
90	47.5
91	33.0
92	26.0
93	26.0
94	16.0
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	56786.0
25	46333.0
26	55090.0
27	85419.0
28	79809.0
29	91474.0
30	206165.0
31	304197.0
32	279735.0
33	67998.0
34	59597.0
35	59168.0
36	76221.0
37	101343.0
38	45682.0
39	51824.0
40	54210.0
41	66381.0
42	100222.0
43	197922.0
44	735990.0
45	1490330.0
46	2103891.0
47	2366558.0
48	3371363.0
49	3620634.0
50	3091700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.577293696485611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.06386089241954	5.838037249910235
2	12.845934867262244	2.203667123248663
3	5.5276939080499305	1.4223796234085573
4	2.842644801375434	0.9752879734474039
5	1.7317548510450003	0.742688498386333
6	1.1439750370557609	0.5887325924565162
7	0.8067878964380437	0.4844039716963242
8	0.6229304270980847	0.4274445780557992
9	0.4948792112330914	0.3820251905128229
>10	3.7831865815048062	6.31622464889494
>50	0.4659174996091944	2.8446580907931245
>100	1.2989426870913605	30.726020147898915
>500	0.28000260076418926	15.483447015959323
>1k	0.07889346635471468	12.517789011130576
>5k	0.007474117865183497	4.286241092905279
>10k+	0.005121154833551655	14.760953191295206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	207476	1.0997325247129206	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	110223	0.5842401919809147	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	91445	0.4847068611423636	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	79546	0.4216358682971235	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	60805	0.32229865702620614	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	54563	0.2892127559135085	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	51847	0.2748165195434209	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	43900	0.23269321673300633	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	37019	0.1962202776819854	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	32856	0.1741541760587621	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	31415	0.16651611397875613	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	30155	0.15983744762149898	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	29880	0.15837980218638334	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	27807	0.1473918058700389	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21722	0.11513808778757093	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21666	0.11484125817169283	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	21583	0.11440131427673064	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21346	0.11314508893810371	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	20788	0.11018739383703269	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA	20115	0.10662013791764059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.020788674169176555	0.0	0.0	0.0	0.0
2	0.03216360909193354	0.0	0.0	0.0	0.0
3	0.0457859682491961	0.0	0.0	0.0	0.0
4	0.07838952123609182	0.0	0.0	0.0	0.0
5	0.09552083049534184	0.0	0.0	0.0	0.0
6	0.14923638991156704	0.0	0.0	0.0	0.0
7	0.16970703234944565	0.0	0.0	0.0	0.0
8	0.19980873571679741	0.0	0.0	0.0	0.0
9	0.2339123383696485	0.0	0.0	0.0	0.0
10	0.2564024823012691	0.0	0.0	0.0	0.0
11	0.2801647531580816	0.0	0.0	0.0	0.0
12	0.3071020407990187	0.0	0.0	0.0	0.0
13	0.33148447353186217	0.0	0.0	0.0	0.0
14	0.35544816448516336	0.0	0.0	0.0	0.0
15	0.38241195477037526	0.0	0.0	0.0	0.0
16	0.4103987471245956	0.0	0.0	0.0	0.0
17	0.4412796282336274	0.0	0.0	0.0	0.0
18	0.4681957137591446	0.0	0.0	0.0	0.0
19	0.49497928606328767	0.0	0.0	0.0	0.0
20	0.523904272024837	0.0	0.0	0.0	0.0
21	0.5504281184150868	0.0	0.0	0.0	0.0
22	0.5735861289824331	0.0	0.0	0.0	0.0
23	0.5987318378703916	0.0	0.0	0.0	0.0
24	0.6238563446429304	0.0	0.0	0.0	5.300528854965975E-6
25	0.646818235642643	0.0	0.0	0.0	5.300528854965975E-6
26	0.6757167189599175	0.0	0.0	0.0	5.300528854965975E-6
27	0.6978888311602401	0.0	0.0	0.0	5.300528854965975E-6
28	0.7269092266411789	0.0	0.0	0.0	5.300528854965975E-6
29	0.7537034000030319	0.0	0.0	0.0	5.300528854965975E-6
30	0.7800735310564876	0.0	0.0	0.0	5.300528854965975E-6
31	0.8051185298962019	0.0	0.0	0.0	5.300528854965975E-6
32	0.83032784513042	0.0	0.0	0.0	5.300528854965975E-6
33	0.8567668830589903	0.0	0.0	0.0	5.300528854965975E-6
34	0.8859303928190131	0.0	0.0	0.0	5.300528854965975E-6
35	0.9093004245405581	0.0	0.0	0.0	5.300528854965975E-6
36	0.9304813378450022	0.0	0.0	0.0	5.300528854965975E-6
37	0.9451213985424182	0.0	0.0	0.0	5.300528854965975E-6
38	0.9570528889949466	0.0	0.0	0.0	5.300528854965975E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTG	70	0.0	245.35657	44
ACTTCTA	27750	0.0	137.35547	44
CTATCAT	31335	0.0	136.71375	44
ATCGACA	130	0.0	132.11507	44
ACGGTTA	85	0.0	129.89465	44
AATCGCA	1490	0.0	117.73822	44
TAGCGTG	335	0.0	113.523186	44
GAACACA	13825	0.0	100.62724	44
ACCACTC	4100	0.0	94.55204	44
CCTTAGC	49275	0.0	90.75254	43
CCAGGCG	1550	0.0	84.68759	44
CGCGTCA	260	0.0	82.59513	43
CCCATAC	12300	0.0	79.49153	44
GCGATAT	50	1.4097168E-9	79.11745	43
CATGACC	3785	0.0	78.76042	44
CACGCGT	105	0.0	75.349945	43
TCCGGGC	4155	0.0	75.213936	43
GACTCGT	245	0.0	75.10915	44
TCGTAAC	95	0.0	71.38416	43
TTGCGTA	145	0.0	70.010376	42
>>END_MODULE