FastQCFastQC Report
Tue 24 May 2016
ERR841088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531638
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC83570.5456250106095566No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA49020.3200495156166144No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA44680.29171383838739967No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA44130.2881229115495959No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT38590.2519524848560822No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC38110.24881858507036259No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG36310.2370664608739141No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA34740.2268159969914562No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG31200.20370348607177416No Hit
ATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30690.20037371754944708Illumina Single End Adapter 2 (97% over 34bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG26390.17229919863570897No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT26340.17197275074136317No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG26180.17092811747945663No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC24580.1604817848603913No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC23440.15303877286930725No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC22030.14383294224875592No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGGTTTAAGTAAGTTC20690.13508413868028868No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC19960.13031799942284011No Hit
AGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGCAG19870.1297303932130177No Hit
AGTACAGCCCAGGTAATCTGCTGAGCCTGATGGGTGTCAGGGTCAGTCC19750.1289469182665878No Hit
ACGGCACCAGTTTTCAGGCTTGGGCTTTTAGGTGGTGCCATTTATTGG19660.12835931205676537No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC19570.12777170584694295No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT19550.12764112668920463No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG19140.12496425395556914No Hit
CTTCAGAAATAAGCCTCTGGCGGCGCGACAGAGCAAAACTCCGTCAGG18350.11980637722490563No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA18300.11947992933055984No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGGTCT18160.11856587522639161No Hit
ATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18020.1176518211222234Illumina Single End Adapter 2 (97% over 34bp)
AGGAAAGGCACTTGCTGCTGAGGCAGG17910.11693363575466266No Hit
AGGTTTTAACAGAGTCGCTAGGCTGCTGTGTTGAGAATAGCCTTTTGGG17900.1168683461757935No Hit
ACAAGCTGGATTTTTGACGCAGGAATAAAAACTGGATTCTGCTCTCTTT17720.11569313375614865No Hit
AGCTGAGATCGCACCATTGCACTCCAACCTGGGCAACAAGAGTGAAACT17620.11504023796745706No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGG17450.11393031512668136No Hit
AGGTGTGGGTGAGAGTTGGAAACATAAGGGATAGTGCGGTTGGTCTTCT17080.1115146007085225No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG16950.11066583618322345No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGCT16810.10975178207905524No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC16670.10883772797488701No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG16430.10727077808202722No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGGTATCATTTATT16310.10648730313559732No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC16200.10576911776803657No Hit
TAACACCTGGACCGAACTCGCTAGCTAACTGGGGGAGATTTTTGTTAAT16140.10537738029482162No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA15780.10302695545553194No Hit
ACGTCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC15730.10270050756118612No Hit
ACAGTGCAAGGCAGGATGTAAAAGTGCTAGGAAGGCACGCTTGATGTGGG15690.10243934924570951No Hit
AGCGCGGGGCAGGAGGAGTACCTCGGCCAAGAAAATTATGCATGCGTTA15410.10061124103737307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC252.2555469E-9231.588244
TGTGCCC900.0231.5881844
ATTCCAC400.0231.5881844
GTATCGC202.064844E-7231.5881844
ACCCTTA950.0219.3993244
TCAATTT900.0218.7221744
TTTCCTA650.0213.773744
GTGCATC1150.0211.4500944
ACCAACC1050.0209.5321744
CAGGATA500.0208.4293844
TAGACAT1400.0206.7751644
GTGTAGC850.0204.3425344
TCGTGCT1200.0202.6396544
CAGGTGC1300.0187.0519944
GCTATAT500.0185.2705544
GTTGACC900.0180.1241544
GATGGGG1350.0180.1241544
GACTTAT401.8371793E-10173.6911344
TGAACAT750.0169.8313344
GATGGTA1250.0166.7434844
Produced by FastQC (version 0.11.5)