##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841088.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531638
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.32309723315823	39.0	37.0	39.0	35.0	39.0
2	37.868318754170375	39.0	38.0	39.0	35.0	39.0
3	38.12805310393187	39.0	38.0	39.0	35.0	40.0
4	38.36819078659579	39.0	38.0	40.0	35.0	41.0
5	39.09229204289787	40.0	38.0	41.0	35.0	41.0
6	39.10424525899723	40.0	38.0	41.0	35.0	41.0
7	39.07716444747388	40.0	38.0	41.0	35.0	41.0
8	39.02655066014293	40.0	38.0	41.0	35.0	41.0
9	38.994579659162284	40.0	38.0	41.0	35.0	41.0
10	38.81885406342752	40.0	38.0	41.0	35.0	41.0
11	38.742100287404725	40.0	38.0	41.0	35.0	41.0
12	38.76453313380838	40.0	38.0	41.0	35.0	41.0
13	38.66588972067812	40.0	38.0	41.0	35.0	41.0
14	38.66514280789586	40.0	38.0	41.0	35.0	41.0
15	38.66985867417758	40.0	38.0	41.0	35.0	41.0
16	38.6749264512894	40.0	38.0	41.0	35.0	41.0
17	38.736048596339344	40.0	38.0	41.0	35.0	41.0
18	38.72608475370812	40.0	38.0	41.0	35.0	41.0
19	38.65391757060089	40.0	38.0	41.0	35.0	41.0
20	38.65475588879357	40.0	37.0	41.0	35.0	41.0
21	38.56541819933953	40.0	37.0	41.0	35.0	41.0
22	38.49447258425294	40.0	37.0	41.0	35.0	41.0
23	38.353804880787756	40.0	36.0	41.0	34.0	41.0
24	38.26594926477405	40.0	36.0	41.0	34.0	41.0
25	38.19810175557065	40.0	36.0	41.0	34.0	41.0
26	38.12937997927199	40.0	36.0	41.0	34.0	41.0
27	38.05435549711946	39.0	36.0	41.0	34.0	41.0
28	37.92335686435433	39.0	35.0	41.0	34.0	41.0
29	37.85517557845095	39.0	35.0	41.0	34.0	41.0
30	37.73921339519229	39.0	35.0	41.0	34.0	41.0
31	37.62375732459195	39.0	35.0	41.0	34.0	41.0
32	37.53247119802646	39.0	35.0	41.0	33.0	41.0
33	37.38705360604349	39.0	35.0	41.0	33.0	41.0
34	37.38167533893767	39.0	35.0	41.0	33.0	41.0
35	37.293035883639185	38.0	35.0	41.0	33.0	41.0
36	37.2365247628276	38.0	35.0	41.0	33.0	41.0
37	37.107361401037146	38.0	35.0	41.0	33.0	41.0
38	37.0367869807965	38.0	35.0	41.0	33.0	41.0
39	37.07565164703278	38.0	35.0	41.0	33.0	41.0
40	36.9399546111057	37.0	35.0	40.0	33.0	41.0
41	36.88007827436846	37.0	35.0	40.0	33.0	41.0
42	36.74704060663946	37.0	35.0	40.0	33.0	41.0
43	36.61397455639454	36.0	35.0	40.0	33.0	41.0
44	36.471428178664766	36.0	35.0	40.0	32.0	41.0
45	36.38786343969517	36.0	35.0	40.0	32.0	41.0
46	36.106347662650776	36.0	35.0	39.0	32.0	41.0
47	36.014565733649974	36.0	35.0	39.0	31.0	41.0
48	35.93062329370491	36.0	34.0	39.0	31.0	41.0
49	36.16580542095761	37.0	34.0	39.0	31.0	41.0
50	36.11472871130824	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	0.0
22	14.0
23	29.0
24	83.0
25	205.0
26	506.0
27	1248.0
28	2581.0
29	5138.0
30	9559.0
31	16560.0
32	28188.0
33	49485.0
34	98330.0
35	96474.0
36	139444.0
37	236298.0
38	253552.0
39	271926.0
40	322015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	58.47106169995783	14.433044883973889	27.09589341606829
2	27.194480680160716	23.33945749583126	32.011872257021565	17.454189566986457
3	36.531347485502444	16.278650699447258	21.449454766726863	25.74054704832343
4	20.283970494333516	27.27726786616681	26.80000104463326	25.63876059486641
5	21.951335759494086	27.556576684569066	26.618953042429084	23.87313451350776
6	25.487941667678655	28.35324012593054	25.038879944216585	21.11993826217422
7	26.315095342372025	25.796696086150906	25.877589874369793	22.01061869710728
8	26.942332326568025	29.303072919319057	23.288009307682366	20.466585446430553
9	27.620495182281974	29.63990185670504	23.272274519174896	19.467328441838085
10	29.36940713145012	27.473332471510894	25.325762353767665	17.83149804327132
11	25.45262000551044	28.132300191037306	26.784070387389185	19.631009416063065
12	24.610449727677167	24.677110387702577	28.843173125764704	21.869266758855552
13	23.75143473849565	26.41492310846297	25.806163075086936	24.02747907795445
14	19.719607374588513	30.400133713057524	27.418685093997407	22.461573818356555
15	22.37584860130135	33.338687078800604	25.487092903153357	18.79837141674469
16	22.840253375797676	34.558818728707436	21.887808999254393	20.713118896240495
17	26.221731244589126	34.04322692437769	21.649371457224227	18.085670373808956
18	24.581069417186043	29.858426077180116	22.10509271773095	23.455411787902886
19	25.797348981939592	29.075277578644567	23.071639643309975	22.05573379610587
20	26.45259519547047	27.986769719737953	22.213734576969234	23.346900507822344
21	24.58916532496582	31.014965677268385	23.277693554221038	21.118175443544754
22	27.54227826679672	29.672285487824148	22.357763387954595	20.427672857424536
23	27.730769280991986	27.666459045805862	24.173727734621366	20.429043938580786
24	25.753996701570475	26.595252925299583	26.09056448064099	21.560185892488956
25	24.827536827833736	30.66514095933009	27.449791572787934	17.057530640048242
26	22.911639596304823	32.02636096804563	25.852327418202147	19.209672017447396
27	26.059694852371628	25.537477440975792	25.17110432299094	23.231723383661638
28	22.210468184491912	29.106565081581387	26.237743473455822	22.445223260470883
29	25.29883840887472	28.340143892603066	25.131566607269658	21.229451091252564
30	26.20811389743109	30.83411825704459	24.72014126604093	18.237626579483386
31	27.552694600608046	28.774360106663043	22.254394755167553	21.418550537561355
32	25.22735695950357	30.411061382782457	23.61744703910163	20.74413461861234
33	24.94994683148905	27.808931202162185	27.483550991925032	19.75757097442373
34	21.57716412898307	30.194583280680675	26.625605738290343	21.602646852045915
35	23.435596029751533	28.621078788663347	27.054001345179163	20.88932383640595
36	26.272899710158086	27.25585110382526	26.19749541402846	20.27375377198819
37	22.22930930311803	27.09734642510459	26.995764932264688	23.677579339512693
38	21.53753944176734	29.927678330354617	26.05939917493505	22.475383052942995
39	23.581932161126065	27.4685100383356	27.172399529252743	21.777158271285582
40	22.323509820459588	28.200690139823298	27.709648840619323	21.76615119909779
41	21.476898230795925	29.664394224612955	25.087657125258882	23.771050419332237
42	20.51007466480246	31.25754644379286	25.275456794081435	22.956922097323243
43	21.242432811315137	27.340887458437184	29.496715853236854	21.919963877010822
44	22.1584356828537	29.984527288029195	24.97307931406856	22.88395771504855
45	20.03193194735666	27.22476847724627	26.52005508623965	26.223244489157416
46	20.463430922911495	29.49919132977979	29.194854979654817	20.8425227676539
47	25.897270325799827	25.866211276249434	29.06134429116525	19.175174106785494
48	25.271016804197583	26.54564973405553	23.521253727203383	24.6620797345435
49	20.954148854230766	30.008639625724893	23.929725610377332	25.10748590966701
50	22.039504595087298	29.326617677460582	27.510007705463362	21.12387002198876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5360.0
1	6237.0
2	8322.5
3	9282.0
4	8542.0
5	7584.5
6	6769.5
7	8714.0
8	14569.5
9	20045.5
10	23985.5
11	25462.0
12	23642.0
13	19565.5
14	14936.5
15	11467.5
16	8597.5
17	6845.0
18	6635.0
19	7299.0
20	7723.5
21	8180.0
22	8594.5
23	8299.5
24	7805.0
25	9561.5
26	12145.5
27	11620.5
28	11046.5
29	15183.5
30	20804.0
31	24933.0
32	29489.5
33	35194.0
34	45301.5
35	53358.5
36	52549.0
37	52350.0
38	62039.5
39	76764.0
40	84740.5
41	96694.0
42	111821.0
43	116211.0
44	124085.5
45	131771.5
46	126801.0
47	121088.5
48	130260.0
49	150740.0
50	172272.5
51	174298.5
52	152301.5
53	130442.0
54	114911.5
55	106276.5
56	101290.5
57	87449.5
58	69579.5
59	58834.0
60	51119.5
61	40105.5
62	29130.5
63	22030.0
64	18945.5
65	18035.0
66	13495.0
67	8107.0
68	5640.0
69	3867.5
70	2345.0
71	1513.5
72	816.0
73	523.5
74	296.5
75	158.0
76	112.5
77	69.5
78	35.5
79	32.5
80	30.0
81	19.5
82	10.0
83	5.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9284.0
25	5566.0
26	6306.0
27	9343.0
28	8359.0
29	9621.0
30	11234.0
31	12216.0
32	15245.0
33	12121.0
34	10971.0
35	11638.0
36	12826.0
37	15441.0
38	8331.0
39	8485.0
40	7374.0
41	9788.0
42	14266.0
43	23821.0
44	69891.0
45	110497.0
46	192089.0
47	158191.0
48	287972.0
49	224717.0
50	266045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.841252241361564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.91537298885905	5.916156914192951
2	12.615360701024098	2.2307117214702776
3	5.689962280812135	1.5091917530547914
4	3.1444366380365985	1.11203029895442
5	1.9618774491741515	0.8672726697393838
6	1.2475202354929964	0.6617784646917818
7	0.8902828459925516	0.5509850645304178
8	0.6707136539710556	0.474395887718672
9	0.5173793431770786	0.4116853149748668
>10	3.7856175645217407	6.81259961415704
>50	0.5928178316815305	3.730842251172259
>100	1.406184619469298	31.925179057946668
>500	0.4388776207080493	27.29095697059074
>1k	0.12285612991152814	15.959383269957472
>5k	7.400971681417359E-4	0.5468307468482483
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	8357	0.5456250106095566	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4902	0.3200495156166144	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4468	0.29171383838739967	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4413	0.2881229115495959	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3859	0.2519524848560822	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	3811	0.24881858507036259	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	3631	0.2370664608739141	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	3474	0.2268159969914562	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3120	0.20370348607177416	No Hit
ATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3069	0.20037371754944708	Illumina Single End Adapter 2 (97% over 34bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	2639	0.17229919863570897	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2634	0.17197275074136317	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	2618	0.17092811747945663	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC	2458	0.1604817848603913	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2344	0.15303877286930725	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	2203	0.14383294224875592	No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGGTTTAAGTAAGTTC	2069	0.13508413868028868	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGAC	1996	0.13031799942284011	No Hit
AGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGCAG	1987	0.1297303932130177	No Hit
AGTACAGCCCAGGTAATCTGCTGAGCCTGATGGGTGTCAGGGTCAGTCC	1975	0.1289469182665878	No Hit
ACGGCACCAGTTTTCAGGCTTGGGCTTTTAGGTGGTGCCATTTATTGG	1966	0.12835931205676537	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1957	0.12777170584694295	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	1955	0.12764112668920463	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1914	0.12496425395556914	No Hit
CTTCAGAAATAAGCCTCTGGCGGCGCGACAGAGCAAAACTCCGTCAGG	1835	0.11980637722490563	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	1830	0.11947992933055984	No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGGTCT	1816	0.11856587522639161	No Hit
ATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1802	0.1176518211222234	Illumina Single End Adapter 2 (97% over 34bp)
AGGAAAGGCACTTGCTGCTGAGGCAGG	1791	0.11693363575466266	No Hit
AGGTTTTAACAGAGTCGCTAGGCTGCTGTGTTGAGAATAGCCTTTTGGG	1790	0.1168683461757935	No Hit
ACAAGCTGGATTTTTGACGCAGGAATAAAAACTGGATTCTGCTCTCTTT	1772	0.11569313375614865	No Hit
AGCTGAGATCGCACCATTGCACTCCAACCTGGGCAACAAGAGTGAAACT	1762	0.11504023796745706	No Hit
ATCTACCTGAGGAAAGGCACTTGCTGCTGAGGCAGG	1745	0.11393031512668136	No Hit
AGGTGTGGGTGAGAGTTGGAAACATAAGGGATAGTGCGGTTGGTCTTCT	1708	0.1115146007085225	No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG	1695	0.11066583618322345	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGCT	1681	0.10975178207905524	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	1667	0.10883772797488701	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	1643	0.10727077808202722	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGGTATCATTTATT	1631	0.10648730313559732	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	1620	0.10576911776803657	No Hit
TAACACCTGGACCGAACTCGCTAGCTAACTGGGGGAGATTTTTGTTAAT	1614	0.10537738029482162	No Hit
ATGCCAAAAAAAAAAAAAAAAAAAAAAAAAA	1578	0.10302695545553194	No Hit
ACGTCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	1573	0.10270050756118612	No Hit
ACAGTGCAAGGCAGGATGTAAAAGTGCTAGGAAGGCACGCTTGATGTGGG	1569	0.10243934924570951	No Hit
AGCGCGGGGCAGGAGGAGTACCTCGGCCAAGAAAATTATGCATGCGTTA	1541	0.10061124103737307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08951201262961614	0.0	0.0	0.0	0.0
2	0.1242460685880084	0.0	0.0	0.0	0.0
3	0.21323576458667126	0.0	0.0	0.0	0.0
4	0.36921256850509065	0.0	0.0	0.0	0.0
5	0.43097651011531446	0.0	0.0	0.0	0.0
6	0.5432745857702669	0.0	0.0	0.0	0.0
7	0.6709810020383407	0.0	0.0	0.0	0.0
8	0.8020824764076107	0.0	0.0	0.0	0.0
9	0.9927280467055531	0.0	0.0	0.0	0.0
10	1.081717742704216	0.0	0.0	0.0	0.0
11	1.159216472821907	0.0	0.0	0.0	0.0
12	1.2126233483368785	0.0	0.0	0.0	0.0
13	1.2688376757432238	0.0	0.0	0.0	0.0
14	1.3403950541838214	0.0	0.0	0.0	0.0
15	1.4075780308401855	0.0	0.0	0.0	0.0
16	1.455631160887886	0.0	0.0	0.0	0.0
17	1.594567384721455	0.0	0.0	0.0	0.0
18	1.6577024074879312	0.0	0.0	0.0	0.0
19	1.7526334551636875	0.0	0.0	0.0	0.0
20	1.797748554162276	0.0	0.0	0.0	0.0
21	1.848021529891528	0.0	0.0	0.0	0.0
22	1.8834084816386116	0.0	0.0	0.0	0.0
23	1.934595511472032	0.0	0.0	0.0	0.0
24	1.982452772783125	0.0	0.0	0.0	0.0
25	2.0147711143233584	0.0	0.0	0.0	0.0
26	2.086328492763956	0.0	0.0	0.0	0.0
27	2.114011274204479	0.0	0.0	0.0	0.0
28	2.1650024353012918	0.0	0.0	0.0	0.0
29	2.2138390402954222	0.0	0.0	0.0	0.0
30	2.2436763778386277	0.0	0.0	0.0	0.0
31	2.2744277694860013	0.0	0.0	0.0	0.0
32	2.299890705244973	0.0	0.0	0.0	0.0
33	2.3289445678417486	0.0	0.0	0.0	0.0
34	2.3571496659132247	0.0	0.0	0.0	0.0
35	2.3816985475680283	0.0	0.0	0.0	0.0
36	2.4035705564891967	0.0	0.0	0.0	0.0
37	2.4163673139475517	0.0	0.0	0.0	0.0
38	2.4298822567734675	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTC	25	2.2555469E-9	231.5882	44
TGTGCCC	90	0.0	231.58818	44
ATTCCAC	40	0.0	231.58818	44
GTATCGC	20	2.064844E-7	231.58818	44
ACCCTTA	95	0.0	219.39932	44
TCAATTT	90	0.0	218.72217	44
TTTCCTA	65	0.0	213.7737	44
GTGCATC	115	0.0	211.45009	44
ACCAACC	105	0.0	209.53217	44
CAGGATA	50	0.0	208.42938	44
TAGACAT	140	0.0	206.77516	44
GTGTAGC	85	0.0	204.34253	44
TCGTGCT	120	0.0	202.63965	44
CAGGTGC	130	0.0	187.05199	44
GCTATAT	50	0.0	185.27055	44
GTTGACC	90	0.0	180.12415	44
GATGGGG	135	0.0	180.12415	44
GACTTAT	40	1.8371793E-10	173.69113	44
TGAACAT	75	0.0	169.83133	44
GATGGTA	125	0.0	166.74348	44
>>END_MODULE