FastQCFastQC Report
Tue 24 May 2016
ERR841087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3399039
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC288280.848122072150393No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA177340.5217357023558717No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA167160.4917860607071587No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG126750.37289951659866216No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT112620.33132894326896517No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA104240.30667491605715613No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT86630.25486615481611125No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC86110.2533363106454501No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT85350.2511003845498684No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC75890.2232689886759169No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG75430.22191566498648588No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC67370.19820308034123763No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC61850.18196319606806513No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC60910.179197708528793No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG60260.17728540331546652No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA56210.16537027083243233No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA52070.15319035762755298No Hit
TCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA51280.15086617129135618No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT48770.1434817311598955No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA48070.1414223255455439No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT47520.13980422113426766No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.12718300672631294No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.12682996576385266No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT42740.12574142279626685No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG42630.12541780191401158No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT42320.12450577942765588No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT42160.12403505814437552No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT41500.12209333285084403No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT39820.11715075937640022No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA39810.1171213392961952No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTT39340.11573859552655912No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC38720.11391455055384772No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC37240.10956037868350436No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT35860.10550040761521123No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.10114623574486788No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA34330.10099913534384278No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT34270.10082261486261265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT252.8885552E-9222.2744644
CCCGTAC750.0222.2744444
TATCCAC1100.0222.2744444
ACCGGTA1100.0212.1710744
TACATTA1250.0204.492544
GGTCGTT1000.0200.0470144
CATACAG401.8189894E-12194.4901444
CCGAATC800.0194.4901444
TTTAACG1050.0190.5209544
CGTGACA257.7116965E-7177.8195644
CCGCACC1000.0177.8195644
GTAAAGT700.0174.644244
TTCACGG207.0538925E-5166.7058344
ATAACTA1250.0160.037644
CACGCTC700.0158.7674444
CTACCCG850.0156.8996144
ATTAGTT1200.0148.1829744
TATTACT150.006644519148.1829744
AGTTCGG700.0142.890744
ACGAACA4750.0140.3838544
Produced by FastQC (version 0.11.5)