##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841087.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3399039
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.412237105840795	39.0	37.0	39.0	35.0	39.0
2	37.92224684682935	39.0	38.0	39.0	35.0	39.0
3	38.10853302948274	39.0	38.0	39.0	35.0	39.0
4	38.69672722201775	39.0	38.0	41.0	35.0	41.0
5	39.305769071787644	40.0	39.0	41.0	36.0	41.0
6	39.276412244755065	40.0	39.0	41.0	35.0	41.0
7	39.29870354532561	40.0	39.0	41.0	35.0	41.0
8	39.24105519236466	40.0	39.0	41.0	35.0	41.0
9	39.201124494305596	40.0	39.0	41.0	35.0	41.0
10	39.170229585479895	40.0	39.0	41.0	35.0	41.0
11	39.032365324434345	40.0	38.0	41.0	35.0	41.0
12	39.02905468280888	40.0	38.0	41.0	35.0	41.0
13	38.93868619924632	40.0	38.0	41.0	35.0	41.0
14	38.924706953936095	40.0	38.0	41.0	35.0	41.0
15	38.8619424490275	40.0	38.0	41.0	35.0	41.0
16	38.86941485519878	40.0	38.0	41.0	35.0	41.0
17	38.947910865394604	40.0	38.0	41.0	35.0	41.0
18	38.97123863539077	40.0	38.0	41.0	35.0	41.0
19	38.943896789651426	40.0	38.0	41.0	35.0	41.0
20	38.964802698645116	40.0	38.0	41.0	35.0	41.0
21	38.99383708159865	40.0	38.0	41.0	35.0	41.0
22	38.99102452193105	40.0	38.0	41.0	35.0	41.0
23	38.93782419089631	40.0	38.0	41.0	35.0	41.0
24	38.874518650712744	40.0	38.0	41.0	35.0	41.0
25	38.87233114116722	40.0	38.0	41.0	35.0	41.0
26	38.83023923110052	40.0	38.0	41.0	35.0	41.0
27	38.78256738977222	40.0	38.0	41.0	35.0	41.0
28	38.608544892272405	40.0	38.0	41.0	35.0	41.0
29	38.621266380427784	40.0	38.0	41.0	35.0	41.0
30	38.5907918264917	40.0	38.0	41.0	35.0	41.0
31	38.49911520969146	40.0	37.0	41.0	34.0	41.0
32	38.30550124664816	40.0	37.0	41.0	34.0	41.0
33	38.258689206062535	40.0	37.0	41.0	34.0	41.0
34	38.27410807041694	40.0	37.0	41.0	34.0	41.0
35	38.26385026593155	40.0	37.0	41.0	34.0	41.0
36	38.25024553838783	40.0	37.0	41.0	34.0	41.0
37	38.248677393194825	40.0	36.0	41.0	34.0	41.0
38	38.12935590943204	40.0	36.0	41.0	34.0	41.0
39	38.07205283559832	40.0	36.0	41.0	34.0	41.0
40	37.99614719492101	39.0	36.0	41.0	34.0	41.0
41	37.901317041516776	39.0	35.0	41.0	34.0	41.0
42	37.82912615562212	39.0	35.0	41.0	34.0	41.0
43	37.726895429458985	39.0	35.0	41.0	34.0	41.0
44	37.52862255140759	39.0	35.0	41.0	34.0	41.0
45	37.27745964516503	38.0	35.0	41.0	33.0	41.0
46	37.04701516593498	38.0	35.0	40.0	33.0	41.0
47	36.77672015785655	38.0	35.0	40.0	32.0	41.0
48	36.81695653293745	38.0	35.0	40.0	32.0	41.0
49	36.82407965845265	38.0	35.0	40.0	32.0	41.0
50	36.847378798744735	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	12.0
21	27.0
22	57.0
23	116.0
24	264.0
25	484.0
26	948.0
27	1905.0
28	4116.0
29	8131.0
30	15825.0
31	28787.0
32	48339.0
33	86350.0
34	162437.0
35	137643.0
36	222089.0
37	357765.0
38	519500.0
39	760075.0
40	1044167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.15622239109348	21.704781851576282	32.138995757330235
2	24.42419754524735	22.359996457822344	29.494483587861158	23.721322409069153
3	22.35493620402708	22.674791316016083	30.605915377846504	24.364357102110333
4	20.75468978143528	23.56245397596203	34.938404649078755	20.744451593523934
5	17.78985177869392	26.371600914258416	34.57515491878734	21.26339238826033
6	19.640727864552304	26.138829239676276	29.953172058337667	24.267270837433756
7	22.821391575677712	25.639217437634578	25.397119597627448	26.142271389060262
8	25.520654514408335	25.158463907004304	28.7883134027	20.532568175887363
9	23.78304573733929	26.597547130232986	28.528386994088624	21.0910201383391
10	23.532504334313316	24.334201519900184	27.717775524199634	24.415518621586866
11	25.85774979339749	23.61741068578501	27.28988987769779	23.23494964311972
12	23.682046601995445	25.41077051484258	25.757809780941027	25.149373102220952
13	23.896136525647396	24.243705353189533	27.02131396550613	24.838844155656936
14	20.58826038771547	26.80442913423471	26.96906390306201	25.63824657498781
15	21.64705965421403	29.16718519558028	27.846723735738248	21.339031414467442
16	21.987361721945526	30.364288259122652	25.190296433786134	22.458053585145684
17	23.559070666738453	32.314692476314626	22.72807108126738	21.398165775679537
18	22.944308670774298	26.97382995605523	25.123807052522785	24.958054320647687
19	23.63468027286536	24.693244178722278	24.962879213801312	26.709196334611047
20	23.115327597006093	23.50287831354686	29.015936563246257	24.365857526200788
21	22.607478172506994	28.84250519043765	26.066926563655198	22.48309007340016
22	25.22818949709021	27.820392763954754	22.894500474987193	24.056917263967843
23	28.23109708361687	25.039400842414576	25.527891854138772	21.20161021982978
24	23.79769693728139	28.801699539193283	25.517506565826398	21.883096957698925
25	25.359107534315378	26.722179875796947	24.885073771412028	23.033638818475648
26	24.307571657212822	31.39608621526291	23.819386688528557	20.476955438995713
27	26.249759734657662	27.800301877571208	23.63442119520274	22.315517192568386
28	25.12059976955477	25.343185501809334	25.334426063289346	24.20178866534655
29	25.93880208726761	25.96723461214229	24.074976054435744	24.018987246154357
30	25.853212388112333	28.10278619647199	26.360382722070717	19.683618693344958
31	24.34294134124679	28.517029945731835	25.138899010295546	22.00112970272583
32	21.742484828527072	26.988286211600887	29.644038826425806	21.62519013344624
33	22.279363592776804	25.579330979605746	32.28831704218662	19.852988385430827
34	23.68780442077066	27.590907281443062	27.11792745395405	21.603360843832224
35	22.278094319464746	28.305123977812496	25.103596926554662	24.313184776168097
36	21.63576799940436	28.75262614393049	26.952604778691736	22.65900107797341
37	21.801047420046913	31.120133343478468	23.473758895689166	23.605060340785457
38	23.495960486708505	28.636615663189506	24.2584151489835	23.60900870111849
39	19.85577890996084	29.838754630689213	27.968477540214614	22.33698891913533
40	20.401606399275632	28.03137782363563	28.11073705378529	23.456278723303452
41	21.386181075201744	28.768611446650933	26.98725844436985	22.85794903377748
42	20.425162790543386	31.276748899525973	25.25461004906107	23.043478260869566
43	22.627860924890047	28.37262879499552	26.860935565139414	22.13857471497502
44	24.887290159781138	26.92117475124273	24.097470033662088	24.094065055314044
45	19.982012555613103	29.18792656487974	29.25350360795887	21.57655727154829
46	21.443181469315075	29.4667746292942	25.355624290056184	23.73441961133454
47	21.088120725958845	30.013493873550363	25.888287433280414	23.010097967210378
48	22.436235722462705	30.716344249008255	24.128688677761538	22.718731350767506
49	21.13732349437742	27.427767127098313	25.413798049908582	26.02111132861569
50	20.867673076389014	31.917868863974597	26.628255162600894	20.586202897035495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30501.0
1	31074.0
2	39878.5
3	47546.5
4	43547.5
5	28202.5
6	11688.5
7	8184.5
8	10607.5
9	11955.0
10	11310.5
11	9416.5
12	7349.5
13	5962.5
14	5615.0
15	5850.0
16	7148.5
17	10126.0
18	12041.5
19	12291.0
20	13128.0
21	14305.5
22	16652.5
23	23760.0
24	30047.5
25	33218.5
26	40487.5
27	48607.5
28	49222.0
29	53315.5
30	67369.5
31	87886.5
32	111948.0
33	126697.0
34	150660.5
35	177523.5
36	170987.0
37	157551.0
38	159290.5
39	166755.0
40	178253.0
41	185266.5
42	191408.5
43	204217.5
44	215309.5
45	217904.5
46	226849.5
47	234326.0
48	240796.0
49	260646.0
50	311225.5
51	350036.5
52	324117.5
53	280636.5
54	253989.5
55	242121.5
56	236999.0
57	228656.0
58	214212.5
59	193397.5
60	159321.5
61	121387.0
62	95437.5
63	75841.0
64	56270.0
65	40784.5
66	26881.5
67	17065.0
68	12275.5
69	9701.0
70	7929.5
71	5514.5
72	3617.5
73	2081.0
74	1242.5
75	847.5
76	541.0
77	421.5
78	230.5
79	64.0
80	10.5
81	9.5
82	8.5
83	6.0
84	4.0
85	5.0
86	7.0
87	7.5
88	5.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14259.0
25	13312.0
26	15803.0
27	22118.0
28	23957.0
29	22923.0
30	34484.0
31	63633.0
32	57913.0
33	15705.0
34	13006.0
35	12796.0
36	13764.0
37	14725.0
38	10879.0
39	10418.0
40	12712.0
41	12022.0
42	16978.0
43	31387.0
44	100913.0
45	259492.0
46	355189.0
47	425844.0
48	563020.0
49	649644.0
50	612143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.331692147307065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82158878039185	5.412371627629303
2	10.304500628646936	1.5109885268194292
3	4.074739917602018	0.8962421596860403
4	2.1438488342006794	0.628721586508901
5	1.2677103808904209	0.46472311223169727
6	0.8451493767816194	0.37178250294307547
7	0.6284976815677303	0.32255660615855775
8	0.48816989392327614	0.28632891022632045
9	0.35912139405264965	0.23696707542351994
>10	3.148897929880052	4.850450127909522
>50	0.6347486108429345	3.30630158905824
>100	1.550889511102346	29.78296882652603
>500	0.5696793369484027	29.031476331707793
>1k	0.15470112100187336	17.22464650455976
>5k	0.0051710681114832315	2.58867775394883
>10k+	0.0025855340557416158	3.0847967586629785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	28828	0.848122072150393	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	17734	0.5217357023558717	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	16716	0.4917860607071587	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	12675	0.37289951659866216	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11262	0.33132894326896517	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	10424	0.30667491605715613	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	8663	0.25486615481611125	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	8611	0.2533363106454501	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8535	0.2511003845498684	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	7589	0.2232689886759169	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	7543	0.22191566498648588	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	6737	0.19820308034123763	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	6185	0.18196319606806513	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6091	0.179197708528793	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	6026	0.17728540331546652	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	5621	0.16537027083243233	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	5207	0.15319035762755298	No Hit
TCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA	5128	0.15086617129135618	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT	4877	0.1434817311598955	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA	4807	0.1414223255455439	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	4752	0.13980422113426766	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4323	0.12718300672631294	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4311	0.12682996576385266	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	4274	0.12574142279626685	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG	4263	0.12541780191401158	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	4232	0.12450577942765588	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4216	0.12403505814437552	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT	4150	0.12209333285084403	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	3982	0.11715075937640022	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	3981	0.1171213392961952	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTT	3934	0.11573859552655912	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC	3872	0.11391455055384772	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	3724	0.10956037868350436	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	3586	0.10550040761521123	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3438	0.10114623574486788	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3433	0.10099913534384278	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	3427	0.10082261486261265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0492197941830029	0.0	0.0	0.0	0.0
2	0.07575670652793334	0.0	0.0	0.0	0.0
3	0.10523562689336603	0.0	0.0	0.0	0.0
4	0.16757677684780906	0.0	0.0	0.0	0.0
5	0.1968791767320116	0.0	0.0	0.0	0.0
6	0.280520464754891	0.0	0.0	0.0	0.0
7	0.31041126624319404	0.0	0.0	0.0	0.0
8	0.35560050943810884	0.0	0.0	0.0	0.0
9	0.4074975309197688	0.0	0.0	0.0	0.0
10	0.44068338139103436	0.0	0.0	0.0	0.0
11	0.481753813357246	0.0	0.0	0.0	0.0
12	0.518028772250039	0.0	0.0	0.0	0.0
13	0.5581577616496898	0.0	0.0	0.0	0.0
14	0.5955212635100686	0.0	0.0	0.0	0.0
15	0.6381803798073514	0.0	0.0	0.0	0.0
16	0.6840168647667767	0.0	0.0	0.0	0.0
17	0.7381203922638134	0.0	0.0	0.0	0.0
18	0.7688937961582671	0.0	0.0	0.0	0.0
19	0.8048157140885998	0.0	0.0	0.0	0.0
20	0.8462097669370666	0.0	0.0	0.0	0.0
21	0.8784541748417715	0.0	0.0	0.0	0.0
22	0.9069622325604384	0.0	0.0	0.0	0.0
23	0.9381180974975574	0.0	0.0	0.0	0.0
24	0.968714980910781	0.0	0.0	0.0	0.0
25	1.001341849858151	0.0	0.0	0.0	0.0
26	1.0341746593669563	0.0	0.0	0.0	0.0
27	1.0625356166845983	0.0	0.0	0.0	0.0
28	1.089543250312809	0.0	0.0	0.0	0.0
29	1.1246708260776062	0.0	0.0	0.0	0.0
30	1.1556795906136998	0.0	0.0	0.0	0.0
31	1.1851585109791327	0.0	0.0	0.0	0.0
32	1.2199624658616743	0.0	0.0	0.0	0.0
33	1.2488235645428016	0.0	0.0	0.0	0.0
34	1.2800971098007408	0.0	0.0	0.0	0.0
35	1.3082227064767424	0.0	0.0	0.0	0.0
36	1.332170651763631	0.0	0.0	0.0	0.0
37	1.3499992203678746	0.0	0.0	0.0	0.0
38	1.3664156251222772	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCAT	25	2.8885552E-9	222.27446	44
CCCGTAC	75	0.0	222.27444	44
TATCCAC	110	0.0	222.27444	44
ACCGGTA	110	0.0	212.17107	44
TACATTA	125	0.0	204.4925	44
GGTCGTT	100	0.0	200.04701	44
CATACAG	40	1.8189894E-12	194.49014	44
CCGAATC	80	0.0	194.49014	44
TTTAACG	105	0.0	190.52095	44
CGTGACA	25	7.7116965E-7	177.81956	44
CCGCACC	100	0.0	177.81956	44
GTAAAGT	70	0.0	174.6442	44
TTCACGG	20	7.0538925E-5	166.70583	44
ATAACTA	125	0.0	160.0376	44
CACGCTC	70	0.0	158.76744	44
CTACCCG	85	0.0	156.89961	44
ATTAGTT	120	0.0	148.18297	44
TATTACT	15	0.006644519	148.18297	44
AGTTCGG	70	0.0	142.8907	44
ACGAACA	475	0.0	140.38385	44
>>END_MODULE